Openhackuarium vol. 92
Darty Monkeys: Biofilia Night #1
Introduction to: Silvan Lanz Visual Research (10min) and "Design beyond Design"(10min) Workshop: "Grow my bacteria" (30min)
Grow My Bacteria Workshop
CAUTION This science activity involves the use of boiling water ;) Although your grown bacteria colonies and fungi is likely to be harmless, just as a precaution, after incubated, do not open your sealed Petri dishes. I wouldn't eat much of it! Dispose of the entire sealed plate in the bin.
- ½ teaspoon of beef stock powder
- ¼ cup of water
- 1 teaspoon of sugar
- 1 teaspoon of gelatin/agar agar
- Saucepan for boiling mixture
- 2 x Petri dishes, Spoon, Sticky tape, Felt-tip pen to label petri dishes, Cotton swab (optional)
- 25 to 37oC incubator (optional) – oven, warm spot behind fridge, near a heater, box with a desk lamp inside or on top.
What to do
- Pour the water into the saucepan and bring to the boil.
- Add beef stock powder, sugar and gelatin to the boiling water and stir for a minute until all the ingredients have dissolved.
- Cool your new agar mixture slightly for 10 minutes. The mixture needs to be still hot to avoid the gelatin setting in the saucepan and to prevent contamination from bacteria in the air. The conditions are far from sterile, but you want to avoid as much contamination as possible.
- Take the lid off the Petri dishes and have an adult half-fill the petri dish with the hot mixture. Only take the lid off the petri dish when you are ready to pour your agar, or they will become contaminated with the bacteria in the air.
- Immediately put the lid back on the Petri dish and put it in the fridge for about 4 hours until the agar has set. Do not touch the agar or you will contaminate it with bacteria on your fingers.
- Now it’s time to collect and grow your bacteria (or fungi) on the agar Petri dishes. Note: the Petri dishes can be stored in the fridge for 1-2 days before use.
- Bacteria is not hard to collect because it is everywhere. Try exposing one plate to the air in your house or classroom, and the other to the air in your backyard or playground. Touch one with your thumb and the other with a piece of hair. Add the scrapings from your finger nail, or touch with a piece of grass or dirty tissue. If you have some cotton swabs - using a clean one each time - run it along things like the inside of your mouth, your hands, the door handle, mobile phone, computer keyboard, and then rub it lightly across the agar in a zig-zag pattern.
- Put the lids back on the Petri dishes, label them, tape them closed and place them upside down in your make-do incubator (if you have one) for 1 to 2 days. If you don’t have an incubator, leave the plates at room temperature for 3-5 days.
- Although your grown bacteria colonies and fungi is likely to be harmless, just as a precaution, do not open your sealed Petri dishes. Dispose of the entire sealed plate in the bin.
- Silvan Lanz (ECAL)
- Design Days & Living Instruments team members (HACKUARIUM, WeSPOKE)
- Wednesday 6th April 2016, 7.30pm
- Practical information: see Open Hackuarium.
- Vanessa Lorenzo
So, here they are the photos! Thanks Sylvain Lanz for the photos. Thank you all for coming, letting me experiment with your bacteria and for your colorful bioart. Sorry for the first failed attempt, we made the wrong soup. Luckily we had a ready made soup/medium to continue... Pictures will be uploaded when they're grown in 2 - 3 days. And we made an extra experiment plating and mapping the nano/micro/macro polluted LANDSCAPES of Montreux (We will publish the exact GPS point soon) thanks to Roger Erismann! Looking forward to see what's going on Congrats to Sachiko Kana and her finished, beautiful, self-built and welded white boards! (beautifully painted in purple and Klinik colors). Next week Rachel Aronoff and her genomic integrity http://wiki.hackuarium.ch/w/Openhackuarium_vol._93 And very soon we go to La Triennale di Milano at Istituto Svizzero Milano and We print Tshirts http://wiki.hackuarium.ch/w/Openhackuarium_vol._95 Organized by Anne-Laure Pittet at Hackuarium