Jun scoby
Here is the Jun (green tea/honey) Kombucha 'protocol' from Patrik of counter culture labs:
for 1L you would use 2 tea bags, 60ml of honey (or 50g of sugar for black tea kombucha), and at least 100ml of starter.
Jun purists will say you shouldn't heat your honey so the beneficial microbes in your honey contribute to your kombucha. For simplicity, repeatability, and food safety, I always pour my boiling water right on the honey though. Loose leaf tea is nice, but tea bags simplify things and reduce some contamination opportunities by not having to strain out the leaves.
I never used vinegar with the starter from Patrik...
It took at least 3 weeks to get the very thick initial scoby dried fairly well...
Here is a link with many recipes and
following is the protocol from Theresa (starting with black tea and sugar...)
SCOBY (symbiotic culture of bacteria and yeast) is the mother
Start with clean [sterile] jars. Brew at room temperature (Brew in these jars covered with clean cloth.
Cover with tightly woven cloth, with a rubber band to hold in place.
First Brew.
Set somewhere dark, still, at 21-24 C for 1 to 4 weeks, until a ½ cm thick SCOBY has formed.
Keep SCOBY in its original tea until you’re ready to brew your second batch. Discard the first batch of tea, it will take strongly of vinegar. The next batch will have a much smoother finish.
Your SCOBY will be useful many years of brewing if kept in service without contamination.
If any green, black or white mold shows up on your scoby throw
the whole thing out a start a new culture.
It takes 1 to 4 weeks for your SCOBY to develop.
If you are starting with liquid culture you may need to let it mature before your first brew is ready.
Fermentation occurs in 6 to 10 days with a mature scoby, such as one you been given from another brew.
Let the fermentation continue 6 to 10 more days to develop sparkle.
You may keep the Kombucha on the counter or in your fridge.
You may add fruit juice at this point to mix for flavor.
Pour off your finished brew, bottle your product and start again using the same live SCOBY with fresh sweet tea.