From Hackuarium
Jump to navigation Jump to search



This chapter presents and vulgarizes the process of designing a plasmid All the primer design is made based on the assumption that Gibson ligation or any other method using overhands is used.
For information on what's a primer

Primer Design

Once you have chosen one or several DNA fragments to insert into a vector you have to design the primers that will allow you to ligate or in other words to connect your fragment to your vector in order to generate your plasmid.