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- During a PCR process, often a plasmid is used and part of its sequence is amplified. After the PCR the plasmid is still present and has to be destroyed to avoid contamination further away in the process. This DNA is called methylated genomic DNA (Me-DNA).
- The dPNI enzyme is a restriction enzyme that will cut the Me-DNA
- The buffer is a specific buffer used to give your enzyme the best operating conditions.
- The enzyme has to operate at a specific temperature (often 37°C) and then be denatured (destroyed) by heating it (often 80°C) this is why we use a heat-block or a PCR