BioP Cultures

DIY POTATO DEXTROSE AGAR

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Media Preparation Penn State Spawn Lab Procedure for Making PDYA PDYA: POTATO DEXTROSE YEAST EXTRACT AGAR To Make 1 Liter:

250 grams potatoes; Do not peel. Scrub clean, cutting out eyes and bad spots 20 grams Agar. Cut into 1/2" pieces. 10 grams Dextrose, Anhydrous 1.5 grams Yeast Extract Rinse and drain cut potatoes twice with tap water and once with distilled water. Add 1 liter distilled water to the potatoes. Cook until potatoes are tender. Filter the potato broth through two layers of cheese cloth. Save the broth and discard potatoes. Add distilled water to broth bringing volume up to 1 liter. Heat the 1 liter of broth to a boil. While stirring slowly add agar, dextrose and yeast extract. Stir until dissolved, watch carefully or it will boil over.

Source: [1]

DIY LB MEDIUM AGAR

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52,5 gr beef powder 1 litres ml water 21 gr sugar 37,5 gr agar

Successfully grown: M.Luteus, M.Aurantiaca, M. Roseus {J. Lividum (Chromobacterium Violaceum ) is not growing for some reasons}

Source: [2]