Difference between revisions of "BioP PCR"
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| + | == Introduction == | ||
| + | ::<li>[http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] or Polymerase Chain Reaction is a process used to amplify in an exponential manner a DNA segment (here called template). | ||
| + | ::<li>It uses a polymerase, an enzyme that will read the DNA segment contained in between the two primers and copy it. | ||
| + | ::<li>The [http://en.wikipedia.org/wiki/Nucleotide dNTPs] or di-nucleotide tri-phosphate are the bases used to code the DNA sequence. | ||
| + | ::<li>mQ H2O is some ultra-pure water that should contain no salts | ||
| + | ::<li>The [http://en.wikipedia.org/wiki/Buffer_solution buffer] is a solution specifically made to give the best environment for your polymerase to operate salts (MgSO4) or DMSO could be added to optimize the copy. | ||
| + | ::<li>The PCR Temperature (T°) Setup is a program used to give instructions to your PCR machine and is different depending on the polymerase, the [http://en.wikipedia.org/wiki/Primer_(molecular_biology) primers] & the template you want to amplify. | ||
| + | <br> | ||
| + | == Protocols == | ||
| + | <br> | ||
| + | == Safety == | ||
| + | <br> | ||
| + | == Hackuarium Specifics == | ||
| + | <br> | ||
Latest revision as of 12:58, 17 January 2015
Introduction
- PCR or Polymerase Chain Reaction is a process used to amplify in an exponential manner a DNA segment (here called template).
- It uses a polymerase, an enzyme that will read the DNA segment contained in between the two primers and copy it.
- The dNTPs or di-nucleotide tri-phosphate are the bases used to code the DNA sequence.
- mQ H2O is some ultra-pure water that should contain no salts
- The buffer is a solution specifically made to give the best environment for your polymerase to operate salts (MgSO4) or DMSO could be added to optimize the copy.
- The PCR Temperature (T°) Setup is a program used to give instructions to your PCR machine and is different depending on the polymerase, the primers & the template you want to amplify.
Protocols
Safety
Hackuarium Specifics