Difference between revisions of "BioP PDSI"
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=Primer Design= | =Primer Design= | ||
Once you have chosen one or several DNA fragments to insert into a vector you have to design the primers that will allow you to ligate or in other words to connect your fragment to your vector in order to generate your plasmid. | Once you have chosen one or several DNA fragments to insert into a vector you have to design the primers that will allow you to ligate or in other words to connect your fragment to your vector in order to generate your plasmid. | ||
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Latest revision as of 14:43, 10 November 2026
Introduction
This chapter presents and vulgarizes the process of designing a plasmid
All the primer design is made based on the assumption that Gibson ligation or any other method using overhands is used.
For information on what's a primer
Primer Design
Once you have chosen one or several DNA fragments to insert into a vector you have to design the primers that will allow you to ligate or in other words to connect your fragment to your vector in order to generate your plasmid.