Difference between revisions of "BioP PDSI"
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=Introduction= | =Introduction= | ||
| + | This chapter presents and vulgarizes the process of designing a plasmid | ||
All the primer design is made based on the assumption that Gibson ligation or any other method using overhands is used. <br> | All the primer design is made based on the assumption that Gibson ligation or any other method using overhands is used. <br> | ||
For information on [https://en.wikipedia.org/wiki/Primer_(molecular_biology) what's a primer] | For information on [https://en.wikipedia.org/wiki/Primer_(molecular_biology) what's a primer] | ||
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=Primer Design= | =Primer Design= | ||
Once you have chosen one or several DNA fragments to insert into a vector you have to design the primers that will allow you to ligate or in other words to connect your fragment to your vector in order to generate your plasmid. | Once you have chosen one or several DNA fragments to insert into a vector you have to design the primers that will allow you to ligate or in other words to connect your fragment to your vector in order to generate your plasmid. | ||
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| + | [[Category:Biology protocols]] | ||
Latest revision as of 14:43, 10 November 2026
Introduction
This chapter presents and vulgarizes the process of designing a plasmid
All the primer design is made based on the assumption that Gibson ligation or any other method using overhands is used.
For information on what's a primer
Primer Design
Once you have chosen one or several DNA fragments to insert into a vector you have to design the primers that will allow you to ligate or in other words to connect your fragment to your vector in order to generate your plasmid.