Difference between revisions of "P1 Lab Activities"
(Created page with "200px|thumb| The P1 lab is official at Hackuarium! (as of 1nov17) <br> We can official select for bacterial clones carrying plasmids we construct.<br>...") |
|||
| Line 26: | Line 26: | ||
To learn, have fun, and avoid problems! <br> | To learn, have fun, and avoid problems! <br> | ||
| − | Here is the [https:// link to the P1 safety info, list of users and list of strains] for future reference. <br> | + | Here is the [https://drive.google.com/open?id=0B1jaPHOkH_42dVhjOXBBN0xsUms link to all the P1 safety info, list of users and list of strains] for future reference. <br> |
Watch this space for more soon! <br> | Watch this space for more soon! <br> | ||
| Line 33: | Line 33: | ||
=== Gallery === | === Gallery === | ||
The first images are of plates carrying our first plasmid strains, streaked at UNIL and grown in our own incubator! <br> | The first images are of plates carrying our first plasmid strains, streaked at UNIL and grown in our own incubator! <br> | ||
| + | To note: The bioluminescent strain is supposed to be moderately expressed, but on the plate almost no light is visible by eye... <br> | ||
| + | Vlad helpfully found this in the DMF collection, but more work is planned to make it bright! | ||
| + | The second strain actually carries two plasmids, a constitutive mCherry one, and an arsenic inducible GFP one... By plating on the two different antibiotics, new sub strains will be selected with each plasmid alone, is possible... | ||
| + | |||
<gallery mode=packed widths=300 heights=300> | <gallery mode=packed widths=300 heights=300> | ||
Revision as of 15:29, 2 November 2017
The P1 lab is official at Hackuarium! (as of 1nov17)
We can official select for bacterial clones carrying plasmids we construct.
(If someone would like to help for translations to French or other languages, please do feel free!)
Inspiration
Many Hackuarium members have been working towards this goal since the foundation of this association!
In particular Yann P, Sam S and others did great leg-work, and even tried a 'green house' enclosure in the old space...
(Prior to renovation.)
All the work leading up to us finally getting the lab up and running is documented (sorry some bits are still redundant and in need of edits) in [Pushing_the_P1 this older page].
The How To Grow Almost Anything course, which Dan Hernandez and Thomas Jordon really wanted to do, was further inspiration for finally getting the lab running.
Now (nov 2017 - present), Rachel Aronoff is acting as the primary biosafety officer for the space, and Olivier Emery has agreed to take on the role of deputy biosafety officer.
Six people have already taken the 'intro to biosafety' training, and the next training session is planned for 10nov17.
First P1 experiments at Hackuarium
Key Aims
To learn, have fun, and avoid problems!
Here is the link to all the P1 safety info, list of users and list of strains for future reference.
Watch this space for more soon!
Gallery
The first images are of plates carrying our first plasmid strains, streaked at UNIL and grown in our own incubator!
To note: The bioluminescent strain is supposed to be moderately expressed, but on the plate almost no light is visible by eye...
Vlad helpfully found this in the DMF collection, but more work is planned to make it bright!
The second strain actually carries two plasmids, a constitutive mCherry one, and an arsenic inducible GFP one... By plating on the two different antibiotics, new sub strains will be selected with each plasmid alone, is possible...
Come join us!
Timeline for Project Initiation
1) Talk with everyone about your ideas and what you would like to do.
2) Take the safety course and do risk assessment on your idea.
3) Start documenting your idea in the wiki. You can use this template developed by YannP as a guide.
4) Fill in the form, also developed by YannP!
5) Get going! (ask for a minigrant, if necessary for obtaining reagents or other materials.)
Further information
- The P1 standard operating procedure
- Do you have questions? Maybe you can't reach Rachel or Olivier, or want to find out more before even trying to ask them?? There is a service in the DIYBio community for this Ask a Biosafety Professional! Use it!