Difference between revisions of "HTGAA"

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(Week 3 next generation synthesis)
(Week 3 next generation synthesis)
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The Gel contained:
The Gel contained:
=== Week 3 next generation synthesis ===
=== wk3: next generation synthesis ===
Theory task: design a gene with a detailed workflow of how to build it.
Theory task: design a gene with a detailed workflow of how to build it.

Revision as of 12:29, 27 October 2017


From the official webpage: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany.

It's a 15 week experimental course covering the major Synthetic Biology applications today.

HTGAA course 2017

2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by Daniel Hernandez and Thomas Jordan.

Important Links

Hackuarium Specific Links

Hackuarium Official Course Page: http://bio.fabcloud.io/hackuarium_2017/

Hackuarium Working Assignments Folder: https://drive.google.com/open?id=0B26FGn9YdUxQVmZuaTBNNWlGR0k

General Course Links

Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.

Students: https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0

Curriculum: https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0

Recommended Hardware: https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0

Inventory: https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494

Weekly topics / Experiments

wk1: Principles and Practices

Link to weekly page: http://bio.fabcloud.io/2017/principles_practices_hardware.html

wk2: Bio Design / Plasmid digest

Theory class: George Church spoke about the capacity to modify biological material/organisms.

Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.

Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html

The protocol designed for this experiment is:


The experiment was carried out on wednesday, 11th of of October 2017 with the help of Rachel.

The gel prepared was 20 mL of 1% agarose in 1x TAE buffer. It was prepared by dissolving 0.2g agarose in 20 mL of a 1x TAE buffer solution in the microwave. Upon full dissolution of the agarose, the sample was allowed to cool a bit, SYBR safe dye was added, and the liquid cast into an old horizontal mini gel box tray. A special 'hack' with magnets kept the comb from going too deep into the liquid, as shown. Gel Hack HTGAA.jpg

The Gel contained:

wk3: next generation synthesis

Theory task: design a gene with a detailed workflow of how to build it.

Lab task: Recode a GFP plasmid to another variant of fluorescent protein using a golden gate assembly.

Ideally this task should be started ASAP as soon as the oligos arrive for the golden gate assembly

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