Genomic Integrity 2018

Summer 2018 Genomic Integrity.

Issue

Assessing genomic integrity (showing the amount of damage one's genome has endured) would be of a great help for public health. Unfortunately, the tests allowing to check the genomic integrity can be complex or expensive.

Objectives

Therefore, the objectives of this internship would be to make:

• A ready to use device, the best being automatic, for people (even non-scientist) to use easily;
• Using "kitchen-sink-ingredients" to promote easy to do and safe citizen science;
• Finally, developing an algorithm able to count cells and control different parameters (their sizes, the length of their comet tails, ...).
  

Precisely, we will use two methods Micronuclei Assay 2018 and Comet Cell Assay 2018, find a way to make the procedures as simple as possible using a chip and make an algorithm to handle the results.

For both, we tried to replace all the toxic or hard to find components by "kitchen-sink-ingredients", in order to make this technique widely usable by anyone, even without the appropriate equipment.

The first target was SYBR Safe. <ref>ThermoFischer's documentations for SYBR Safe. Retrieved 27 August 2018.</ref>

Protocol

For the detailed protocol, see Step by step protocol for comet assay

Ready to use chip

Detailed article: Chip for genomic integrity 2018

References

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