Foldscope

The Genomic Integrity team has been looking into the best ways to get the cheek cell assays to be really DIYbio (for DIT research!) and has been testing open-source microscopy and imaging methods at Hackuarium:

(If someone would like to help for translations to French or other languages, please do feel free!)

Inspiration

Many DIY microscopes exist already. This page from Hackteria describes a few options out there, even using old webcam lenses, upside down...
We had great fun playing with variations on the theme, even simple lenses embedded in simple materials (like cork) during the BioFab Convergence... (to add pic) The first Foldscope was developed at Stanford, by the group of Manu Prakash, and was published in PLOSOne.

First Foldscope Tests at Hackuarium

The first attempts to make Foldscopes at Hackuarium relied on vector graphic files found on the internet in Oliver Bosewell's blog, and included in the folder linked below (under Further information).
We used the laser-cutter in the maker space (Made@UC Thanks, Marc!) to cut the pattern, and simple glass beads for lenses (thanks, Sachiko!) and made our first tests on 5May17.
An image of one of our first folded scopes and an image of an old histology slide can also be found in the folder linked below (under Further information). Even though the beads were 'up-cycled' from microbiology experiments (even scratched), the results seemed promising enough that low magnification (140X) borosilicate glass lenses were ordered.
The second build and an image with cheek cells stained with methylene blue is also included in the folder linked below.

Steiner School Student visit

We look forward to welcoming 18 students from the Steiner School (15-18 year old) on 22 May 2017, with their teacher, Veronica Monticone.
There are 20 packets with purple, Hackuarium-embossed pieces for the Foldscope (3 main ones), including a proper 140x lens, ready for the arrival of 18 students at 9am... :)
The handout students will receive includes spaces for developing hypotheses and controls for their chosen hands-on activities, and a new moss life spotting sheet.
They will see the simplest video on Foldscope making from the PLOSOne paper, and be recommended to explore further on YouTube.
They will also see something short on spotting moss life and the particulars of 'menagerie' viewing (most likely setting up a new one for the occasion), and will have opportunities to make, exchange and view slides of many things, even made with paper and scotch tape!

Watch this space for more soon!

Gallery

• MeBlueStainedCheekCellsx140.jpg Methylene Blue stained cheek cells imaged from a Foldscope
• 

  Water Bear from Neuchâtel moss sample.

• 

  Ciliate from Neuchâtel moss sample

and here is a photo album from the morning visit! (hoping to add students' best foldscope images soon!)

One more point to make after this visit, we need to try other materials, perhaps the water-proof polypropylene sheets others have already tried, instead of just thick paper for the Foldscopes. The purple paper used with the students was far from perfect, with some difficulties with 'crumpled' inserts only solved with good forceps!

Hopes to use fluorescence modules for the cheek cell assays.

With a blue LED, a diffuser, and a filter, fluorescence may be possible, which will be especially important for the cheek cell comet assays, if no colored alternative to SYBR-Safe is found. None of these components come for free, of course. Even the 140X borosilicate lenses were 1 USD each (including shipping, at least), for the simplest bright field version of the Foldscope.

Further information

• Foldscope templates used to date (thank you OB, wherever you may be!), and further info, including our first images can be downloaded here.
• The project to assess your own cells for DNA damage at Hackuarium
• website - Action for Genomic Integrity through Research!
• Twitter account for AGiR! @AGIRgenomes