Automatic stage

From Hackuarium
Revision as of 07:49, 29 August 2018 by Vithoo (talk | contribs)
Jump to navigation Jump to search


For the Genomic Integrity 2018 project, and more specifically to analyze the results of the comet cell assay chip for genomic integrity 2018, we needed an automatic moving stage.

One microscope at Hackuarium is already equipped with a camera linked to a camera with a Raspberry Pi. Thus, the idea is to be able to use this camera in order to automatically take pictures of a whole slide for example. This would allow us to count cells on a slide for instance. For the beginning, we would like to have the x and y directions automated, but our setup can be improved to also automate the z direction (focus).

General concept

We will implement the automatic stage on our Zeiss Axiolab E re. This microscope is an upright microscope.

The Zeiss Axiolab E re with the Raspberry Pi camera on top

The microscope possesses two wheels to move the stage in the x and y direction, and two others to focus the sample (z direction).

The first goal of our automatic stage is to be able to move automatically the sample in the xy directions. To do so, two wheels were 3D printed to be put around the original ones.

The new 3D printed microscope wheels