Talk:Pushing the P1

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Keep in mind that for storing competent bacteria, skim milk is superior to glycerol. See the numbers of recoveries after hurricane Katrina here http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2551311/

We can start without -80 °C. In old times, the postdoc would grow bacteria overnight, make them competent in the morning, transform them in the evening.

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