Campaign 2022
This is the wiki page for documenting the latest Montreux Clean Beach Project water quality analyses, MCBP 2022.
After the publication of our open access work, we will be doing another round of sampling and analyses in 2022 - in collaboration with the organisers of the Montreux Jazz. Anyone can come participate!
The first documentation of the Microbiology Experiments around the Montreux Clean Beach Project can be found in this old wiki page.
The first compilation of results can be found in this other page.
The first tests of the latest R-Cards, the more ecological method for such monitoring, were done in Montreux on 19May22, this season's validation day. Cards are incubated for 48h at 35 degrees Celsius for the easiest scoring.
view from the Vernex site
Kid's swimming at the Vernex Site
labelling tubes for first sampling, validation day 2022
first inoculations on site
after O/N incubation of lab set
controls : river water and 1/100 dilution (top); tap water (below)
control Levine plate : river water with metallic green bioindicator colonies
sampling sites - map from the paper
for the launch of the 2022 campaign,thx to Shannon, we got to use the Sauvetage space!
Shannon, Rachel and Laura prepare to get our first samples
For 'surface' water sampling we want to avoid the very surface, so close lids underwater.
Carol gets one of 6 replicates from the Vernex site, to hand off to Laura.
A little cooler helps avoid extra amplification of bacteria in the samples...
Everyone learned how to make sure to inoculate 1ml on the R-Cards.
Everyone got the chance to inoculate the special microbio cards. Do-It-Together is more fun!
After 48h at 35 degrees Celsius, the SVT site was not quite so clean as Vernex
Here is the overview of all the cards from the first sampling day.
The new season will aim for best results, sampling surface water (0.5-1m) again around the bay, at basically the same 3 sites as before, but with 6 replicate samples per site, so we can calculate medians for better statistics.
The campaign is planned to run for 8 weeks, as in other years, from Monday 13 June to Friday 5 August.
With 48h incubation, that means we can do counts during open nights too! =)
Here is the official invitation for the big launch of the MCBP 2022 (in French, bien sûr)!
Participez au lancement de notre campagne 2022 sur la qualité de l’eau du lac !
L'association Hackuarium organise une sêrie de mesures de la qualité de l'eau du Léman.
Comme lors des campagnes précédentes (2016, 2017 et 2020)*, nous effectuerons des tests microbiologiques mesurant un bio-indicateur important de la pollution des eaux usées non traitées, E. coli, qui fait partie de notre propre microbiome. La campagne de mesures s’étendra sur une période de 8 semaines, du lundi 13 juin au vendredi 5 août 2022, avec des échantillonnages supplémentaires pendant le festival de musique, le Montreux Jazz.
Vous êtes cordialement invité-e-s à participer au lancement de cette campagne de surveillance:
Lundi 13 juin 2022, à 16h30
à Montreux, à côté du Sauvetage (La Vigie) devant l’auditorium Stravinsky,
La séance est publique.
Les volontaires d’ Hackuarium vous montreront les techniques de prélèvement des échantillons et la méthode d'analyse de la teneur en bactéries.
Si vous le souhaitez, vous pouvez également apporter votre propre échantillon d'eau dans une bouteille propre, pour effectuer un test.
(Tests limitées à 12 participants et résultats connus après 48 h d'incubation)
Rejoignez-nous dans notre campagne de la recherche participative !
Si vous avez des questions ou si vous voulez participer à la campagne complète, écrivez-nous svp [[1]].
NOTES:
15.06.22 What a splendid day for the Big Launch sampling! 9 people participated (including 2 Hackuarium members). The sampling at the MRD site was slightly shifted towards a bit discharge pipe and included actual surface water, however.
So far some images have been posted in facebook (at Hackuarium), and more have been added come into the gallery above.
18.06.22 The 48h results were pretty good, with counts made last Weds after 48h of incubation.
With the high lake water temperature (already 22 degrees Celsius!), we are not too surprised about somewhat higher baselines than other years on the lake. ( For instance, at SVT the median result of the 6 replicates came out to 150 bioindicator colonies per 100ml water, while VNX was cleaner (17 bioindicator colonies per 100ml on average). The MRD median was a bit scary, but extra controls the next Monday will be done to demonstrate if the shifted site (by discharge pipe) is the reason for this.
Will try to add a chart showing results, in this page, to follow as we get more each week!
- translation help always in demand =)