Genomic Integrity 2018

Summer 2018 Genomic Integrity Project.

Issue

Assessing genomic integrity (showing the amount of damage one's genome has endured) would be of a great help for public health. Unfortunately, the tests allowing to check the genomic integrity can be complex or expensive.

Objectives

Therefore, the objectives of this internship would be:

• To make a ready to use device: Chip for genomic integrity 2018, the best being automatic, for people (even non-scientist) to use easily;
• To use "kitchen-sink-ingredients" to promote easy to do and safe citizen science;
• Finally, to develop an Automatic Cell Counter Algorithm able to count cells and control different parameters (their sizes, the length of their comet tails and recognition of micronuclei).
  

Asides Objectives

• To use a Foldscope<ref>Official website of the Foldcope.Retrieved 2 August 2018.</ref> <ref>Wiki of the hackuarium.Retrieved 28 August 2018.</ref> or a microscope to make photos with a RaspBerry Pi, using an automatic stage;
• DIY Fluorescence Microscope

Procedure

Precisely, we will use two methods Micronuclei Assay 2018 and Comet Cell Assay 2018, find a way to make the procedures as simple as possible using a chip and make an algorithm to handle the results.

For both, we tried to replace all the toxic or hard to find components by "kitchen-sink-ingredients", in order to make this technique widely usable by anyone, even without the appropriate equipment.

Protocol

For the detailed protocol, see Step by step protocol for comet assay

References

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