DIY Fluorescence Microscope

Before the observation of fluorescent process was not possible in the hacker space. It was thus decided that transforming one of the microscope into a fluorescent microscope would be cool.

Results

Overlay

Fluorescence is visible through the Raspberry Pi Camera. An idea then is to implement a code to overlay the picture of the cell and the fluorescent picture. The goal of this overlay would be to confirm that the fluorescence comes from the nucleus.

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  Phase

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  Fluo

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  Overlay

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  Phase

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  Fluo

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  Overlay

Idea to improve the result

We observe that the resulting "phase" image in yellowish, this is due to the yellow lens that have been added. An idea would be to remove this lens to take the "phase" picture and add it only for the "fluo" one.

No more used

This microscope works well to observe fluorescent nucleus but its resolution is not enough to observe comets. Thus it could not be used to assess the Comet Cell Assay 2018.