Open Source Bioreactor
The Project
History
List of contributors
Project Coordinators
Pr. Luc Patiny, ISIC EPFL-UNIL, Lausanne, CH: https://people.epfl.ch/148588
Pr. Julien Wist, DARMN Research Group, Univalle, Cali, CO: http://quimica.univalle.edu.co/index.php/profesores/julien-wist
Pr. Janeth Sanabria, GAOX Research Group, Univalle, Cali, CO: http://pisa.univalle.edu.co/investigacion/
Quentin Cabrol, MSc Micro and Nanosystems EPFL: https://ch.linkedin.com/in/quentincabrol
Current Collaborators, on Oct 2016
Clément Chenevas, Life Science Student EPFL : operation of Bioreactor v3 @ Univalle
Alice Leydier, Life Science Student EPFL : operation of Bioreactor v3 @ Univalle
Daniel Kostro and Michael Zasso, ISIC EPFL-UNIL, Lausanne, CH : Bioreactor NodeJS GUI interface
Past Collaborators
| Emilien Mingard | Eric xxx | Marco Schukraft |
| David Lambalet | Samuel Benketaf | Raffael Tschui |
| Gabriel Laupré | Gael Grosch | Oliver Peric |
Add project contacts here with roles in the project.
Concept
This project is a collaboration between Univalle (Universidad Del Valle - Colombia) and EPFL (Polytechnical school of Lausanne - Switzerland). The goal was to build an Open Source Bioreactor that everyone could assemble and use, from their backyard to the universities around the world.
This Bioreactor can be used for many applications, such as growing bacterias in anaerobic conditions, with an automatised interface. The temperature, pH, weight, conductometry, feeding and flow of gas going in the reactor can be controlled. All the parameters are stored in a card for future use and can be changed or viewed remotely.
Once the Bioreactor is build, all you have to do is choose the conditions of your experiment (pH, temperature, time of sedimentation, flow of gas), and the Bioreactor will do the rest for you !
Central Unit
Requirements Specifications
Mechanical Design
Electrical Design
Embedded Software
Remote Control & Visualizer
User Manual
This Bioreactor is based on the use of parameters that can either be read or changed. The parameters that can be read are the ph, temperature, weight, gas flow and conductometry. Depending on the needs of the experiment, the user can change the target pH and temperature of the solution, as well as the desired gas flow, the time of sedimentation and the level of emptying and filling of the Bioreactor.
Extension Modules
pH regulation and conductometry
The Bioreactor contains an optional pH board, that can be used with a standard gel probe containing reference electrode and a BNC/RCA (Cinch) 2-Wires output. You can choose to regulate or not the pH of your solution, by changing the pH state. In order to regulate the pH, the target pH should be set and solutions of acid and base should be prepared.
Another board can be added for the measure of the conductometry.
Dynamic gas Mix
The Anemometer board addresses the need to control the bioreactor atmosphere. It dispose of 4 flow measurement channels to dynamically analyze the input and output flows of gas in the reactor. It allows to obtain a desired mix out gas bottles with pure content (CH4, N2, CO2,...) and potentially to operate the system in anaerobic conditions by ensuring a constant overpressure. Though it, of course, requires a bit more of material both electronic and fluidic.
External Resources
Media
Cali Summer Internships 2014: http://univalle.epfl.ch/page-111363.html
Collaboration EPFL - Univalle, CODEV: http://univalle.epfl.ch/
Presentation ALAM 2014: https://goo.gl/mMvKWo
ALAM 2014 Cartagena de las Indias: http://alam2014.acmicro.org/
Bioreactor Isobar : https://www.youtube.com/watch?v=AM8Rpxd9WJg
Bioreactor V3.0 2014 : https://www.youtube.com/watch?v=9sCRF4vdtTM
Presentation CODEV 2015: https://goo.gl/ixcJ2y
For Developers
Github Bioreactor V3 (soon to be abandoned): https://github.com/Bioreactor/Bioreactor_v3
Github Bioreactor V4 (in development 30.11.2016): https://github.com/Bioreactor/Bioreactor_v4
Extensive Developer Manual (in development 30.11.2016): https://goo.gl/il9WWs