Difference between revisions of "AGiR! for genomic integrity"
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At the Hackuarium, AGiR! hopes to really get some citizen science action going - developing DIYbio methods to readily assay *your very own* cells for DNA damage, using classic (circa 1980s) techniques. If these can still be sold in 'kits,' maybe it will also be simple to make them available to all for open source efforts? | At the Hackuarium, AGiR! hopes to really get some citizen science action going - developing DIYbio methods to readily assay *your very own* cells for DNA damage, using classic (circa 1980s) techniques. If these can still be sold in 'kits,' maybe it will also be simple to make them available to all for open source efforts? | ||
<br> | <br> | ||
| − | + | Here is the basic plan: using inner cheek cells for micronuclei and comet assays! | |
<br> | <br> | ||
Watch this space for more info and visit<br> | Watch this space for more info and visit<br> | ||
[http://www.genomicintegrity.org the AGiR! site] | [http://www.genomicintegrity.org the AGiR! site] | ||
| + | |||
| + | |||
| + | |||
| + | == Introduction == | ||
| + | === Context === | ||
| + | This is a part of a new Hackuarium project on genomic integrity | ||
| + | It is about experiments related to simple quantitation of DNA damage in human cells. | ||
| + | |||
| + | === People === | ||
| + | Contact and lead: Rachel Aronoff | ||
| + | * | ||
| + | Participants: maybe you?! | ||
| + | * | ||
| + | |||
| + | === Objectives === | ||
| + | |||
| + | |||
| + | ; Objective | ||
| + | ; Setup in short | ||
| + | |||
| + | == Background & Inspiration == | ||
| + | Here is the link to the Prezi Rachel presented (13apr16) at an Open hackuarium night! | ||
| + | |||
| + | |||
| + | === Some theory needed ? === | ||
| + | |||
| + | === Inspiration / References / Similar projects === | ||
| + | Inspiration: good pages | ||
| + | * [http://] | ||
| + | * [Link 2] | ||
| + | |||
| + | == Run / prototype #1== | ||
| + | <!-- Experiment code / name ? --> | ||
| + | |||
| + | === Setup description === | ||
| + | General principle: | ||
| + | |||
| + | [[File:sketch-of-cool-exp-E1.JPG|300px|thumb|right|set up for run 1]] | ||
| + | |||
| + | === Materials & budget === | ||
| + | * What, size | quantity | where to get it | price / unit | ||
| + | |||
| + | === (If it’s a) Construction === | ||
| + | |||
| + | Step 1 in pictures: <br> | ||
| + | <gallery> | ||
| + | File:name-of-file_1.jpg | ||
| + | File:name-of-file_2.jpg | ||
| + | </gallery> | ||
| + | |||
| + | ==== (If it’s an experiment) Parameters ==== | ||
| + | ;To vary | Values | ||
| + | : p1 | | ||
| + | : p2 | | ||
| + | |||
| + | ; Constants (Key elements of set-up) | ||
| + | : c1 | | ||
| + | : c2 | ||
| + | |||
| + | ; To measure/ observe | ||
| + | : m1 | ||
| + | : m2 | ||
| + | |||
| + | === Protocol (Plan) === | ||
| + | ==== Startup ==== | ||
| + | ==== Phase 1 ==== | ||
| + | do what | frequency | duration | ||
| + | |||
| + | === Lab Notebook & News (Real) === | ||
| + | We put our measures here: [link to other doc?] | ||
| + | |||
| + | == Run / prototype #2== | ||
| + | <!-- Experiment code / name ? --> | ||
| + | What's new compared to #1: | ||
| + | |||
| + | == Results / Feedback from use == | ||
| + | == Recommendation + next steps == | ||
| + | |||
| + | [[Category:Work In Progress]] | ||
| + | [[Category:Manual]] | ||
Revision as of 20:19, 14 April 2016
Genomic integrity is a new 'big picture' concept to aid public health efforts and basically includes all the molecular genetic details of cells!
So many things we commonly choose to do can impact genomic integrity, also potentially affecting future generations!
At the Hackuarium, AGiR! hopes to really get some citizen science action going - developing DIYbio methods to readily assay *your very own* cells for DNA damage, using classic (circa 1980s) techniques. If these can still be sold in 'kits,' maybe it will also be simple to make them available to all for open source efforts?
Here is the basic plan: using inner cheek cells for micronuclei and comet assays!
Watch this space for more info and visit
the AGiR! site
Introduction
Context
This is a part of a new Hackuarium project on genomic integrity It is about experiments related to simple quantitation of DNA damage in human cells.
People
Contact and lead: Rachel Aronoff
Participants: maybe you?!
Objectives
- Objective
- Setup in short
Background & Inspiration
Here is the link to the Prezi Rachel presented (13apr16) at an Open hackuarium night!
Some theory needed ?
Inspiration / References / Similar projects
Inspiration: good pages
- [http://]
- [Link 2]
Run / prototype #1
Setup description
General principle:
Materials & budget
- What, size | quantity | where to get it | price / unit
(If it’s a) Construction
Step 1 in pictures:
- Name-of-file 1.jpg
- Name-of-file 2.jpg
(If it’s an experiment) Parameters
- To vary | Values
- p1 |
- p2 |
- Constants (Key elements of set-up)
- c1 |
- c2
- To measure/ observe
- m1
- m2
Protocol (Plan)
Startup
Phase 1
do what | frequency | duration
Lab Notebook & News (Real)
We put our measures here: [link to other doc?]
Run / prototype #2
What's new compared to #1: