Difference between revisions of "Genomic Integrity 2018"
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* Finally, developing an algorithm able to count cells and control different parameters (their sizes, the length of their comet tails, ...). <br> | * Finally, developing an algorithm able to count cells and control different parameters (their sizes, the length of their comet tails, ...). <br> | ||
| − | Precisely, we will use two methods [[Micronuclei Assay 2018]] and [[Comet Cell Assay 2018]], find a way to make the procedures as simple as possible using a chip and make an algorithm to handle the results. | + | Precisely, we will use two methods [[Micronuclei Assay 2018]] and [[Comet Cell Assay 2018]], find a way to make the procedures as simple as possible using a chip and make an algorithm to handle the results. <br> |
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| + | For both, we tried to replace all the toxic or hard to find components by "[[kitchen-sink-ingredients]]", in order to make this technique widely usable by anyone, even without the appropriate equipment. <br> | ||
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| + | The first target was SYBR Safe. <ref>[https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html ThermoFischer's documentations for SYBR Safe]. Retrieved 27 August 2018.</ref> | ||
==Protocol== | ==Protocol== | ||
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==Ready to use chip== | ==Ready to use chip== | ||
''Detailed article: [[Chip for genomic integrity 2018]] '' | ''Detailed article: [[Chip for genomic integrity 2018]] '' | ||
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| + | ==References== | ||
| + | <references /> | ||
Revision as of 12:52, 27 August 2018
Summer 2018 Genomic Integrity.
Issue
Assessing genomic integrity (showing the amount of damage one's genome has endured) would be of a great help for public health. Unfortunately, the tests allowing to check the genomic integrity can be complex or expensive.
Objectives
Therefore, the objectives of this internship would be to make:
- A ready to use device, the best being automatic, for people (even non-scientist) to use easily;
- Using "kitchen-sink-ingredients" to promote easy to do and safe citizen science;
- Finally, developing an algorithm able to count cells and control different parameters (their sizes, the length of their comet tails, ...).
Precisely, we will use two methods Micronuclei Assay 2018 and Comet Cell Assay 2018, find a way to make the procedures as simple as possible using a chip and make an algorithm to handle the results.
For both, we tried to replace all the toxic or hard to find components by "kitchen-sink-ingredients", in order to make this technique widely usable by anyone, even without the appropriate equipment.
The first target was SYBR Safe. <ref>ThermoFischer's documentations for SYBR Safe. Retrieved 27 August 2018.</ref>
Protocol
For the detailed protocol, see Step by step protocol for comet assay
Ready to use chip
Detailed article: Chip for genomic integrity 2018
References
<references />