AGiR! for genomic integrity

Genomic integrity is a new 'big picture' concept to aid public health efforts and basically includes all the molecular genetic details of cells! So many things we commonly choose to do can impact genomic integrity, also potentially affecting future generations!

At the Hackuarium, AGiR! hopes to really get some citizen science action going - developing DIYbio methods to readily assay *your very own* cells for DNA damage, using classic (circa 1980s) techniques. If these can still be sold in 'kits,' maybe it will also be simple to make them available to all for open source efforts?
Here is the basic plan: using inner cheek cells for micronuclei and comet assays!
Watch this space for more info and visit
the AGiR! site

Introduction

Context

This is a part of a new Hackuarium project on genomic integrity.
This wiki page is under construction, and will help keep track of development of simple protocols for two methods for simple quantitation of DNA damage in human cells.

People

Contact and lead:

• Rachel Aronoff

Participants:

• maybe you?!

Main Goal

Genomic integrity is a topic that can be explored in several ways. Comet assay on cheek cells reveals itself to be a nice and easy "workshopable" way of showing people how to assess DNA damage. This experiment can be used as a gate to demonstrate and raise awareness to everyone on the damages of several substances to your own genetic information. In the scope of Hackuarium activities, mammalian cell cultures is yet still a no go. In order to be relevant and test compounds that might cause DNA damages, a test on bacteria or on bulk DNA has to be established. Such an assay would allow us to test substances that could seem suspicious and to publish those effects.

Objectives

• Setup lab equipement for the cheek cell Comet and micronuclei assay

• Microscope
• Mercury Lamp Setup
• Agar plate and cell migration
• Design a transilluminator for cell illumination (UV/Fluo

• Study the feasability of an assay on either bacteria and bulk DNA

Background & Inspiration

Here is the link to the Prezi Rachel presented (13apr16) at an Open hackuarium night! Prezi on DIYbio for Genomic integrity!

Some theory needed ?

more on comet cells and micronuclei to come soon!

Inspiration / References / Similar projects

Inspiration: good pages to come

• [http://]
• [Link 2]

Setup description

General principle:

Materials & budget

• What, size | quantity | where to get it | price / unit

(If it’s an experiment) Parameters

To get baselines first

then To vary | Values

p1 |

p2 |

Constants (Key elements of set-up)

c1 |

c2

To measure/ observe

m1

m2

Protocol (Plan)

Phase 1

do what | frequency | duration

Lab Notebook & News (Real)

We put our measures here: [link to other doc?]

Run / prototype #2

What's new compared to #1: