Difference between revisions of "Worm validations"
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For this experiment, initial worm cultures of two different GMO worm strains, both carrying a marker gene that makes the worms 'Roll' (actually a mutated collagen gene, termed ''rol-6'', on a plasmid with an antibiotic resistance marker) were grown to starvation for one week, and then washed off plates with water. | For this experiment, initial worm cultures of two different GMO worm strains, both carrying a marker gene that makes the worms 'Roll' (actually a mutated collagen gene, termed ''rol-6'', on a plasmid with an antibiotic resistance marker) were grown to starvation for one week, and then washed off plates with water. | ||
| − | + | Here is a [https://drive.google.com/file/d/1LalApnyA56MxREW_ELxDNaYdRltnH4zB/view?usp=sharing 3-second 'live' video of 'Roller' worms] from one of these starved plates, before being washed. | |
| − | + | The liquid containing the worms was distributed to three individual tubes and treated under three conditions: left in the water with | |
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[[File:Plate_imagep3.png|thumb|3 days after treatment|alt=|left]] | [[File:Plate_imagep3.png|thumb|3 days after treatment|alt=|left]] | ||
Revision as of 04:57, 27 November 2023
English version
Here is the link to the French version.
This page is a simplified summary, concerning the validation of the standard operating procedure (SOP) used to inactivate genetically modified organisms (GMOs) with bleach, for use on nematode worms.
Part one of a validation experiment
Part two of a validation experiment
For this experiment, initial worm cultures of two different GMO worm strains, both carrying a marker gene that makes the worms 'Roll' (actually a mutated collagen gene, termed rol-6, on a plasmid with an antibiotic resistance marker) were grown to starvation for one week, and then washed off plates with water.
Here is a 3-second 'live' video of 'Roller' worms from one of these starved plates, before being washed.
The liquid containing the worms was distributed to three individual tubes and treated under three conditions: left in the water with
In the image on the left are shown the secondary worm plates after treatments, 3 days after the treatments.
Without bleach, the numerous 'FXKI' strain nematodes have eaten all their food (top right), and although the bacterial layer is still clearly visible for the TR1CB strain, without bleach there are many worms.
After treatments with 10% or 100% bleach, only one plate, with the strain TR1CB, treated with 10% bleach, still had a few live nematodes.
An important message to take away from the validation: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used according to the SOP on culture plates kills all stages of C. elegans nematodes...
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To note: The titles of many our wiki pages are not always perfect, but the content can be improved by everyone, too. This is the idea of the open science approach - to 'Do-It-Together'. =)