Difference between revisions of "Worm validations"
| Line 8: | Line 8: | ||
[https://wiki.hackuarium.ch/images/5/50/Nematodes2_smaller.pdf Part two of a validation experiment] | [https://wiki.hackuarium.ch/images/5/50/Nematodes2_smaller.pdf Part two of a validation experiment] | ||
| + | |||
| + | [[File:Plate_imagep3.png|thumb|3 jours post traitements|alt=|left]] | ||
| + | |||
| + | In the image on the left, we can see the treated Petri dishes, 3 days after the treatments. | ||
| + | |||
| + | Without bleach, the numerous 'FXKI' strain nematodes have eaten all their food (top right), and although the bacterial layer is still clearly visible for the TR1CB strain, without bleach there are many worms. | ||
| + | |||
| + | After treatments with 10% or 100% bleach, only the 10% TR1CB still had live nematodes. | ||
| + | |||
| + | |||
An important message to take away from the validation: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing according to the SOP] on culture plates kills all stages of ''C. elegans'' nematodes...[[File:Postvalid nematodes.png|thumb|disinfection after the third day of one set of post-validation tests..]] | An important message to take away from the validation: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing according to the SOP] on culture plates kills all stages of ''C. elegans'' nematodes...[[File:Postvalid nematodes.png|thumb|disinfection after the third day of one set of post-validation tests..]] | ||
Revision as of 20:37, 26 November 2023
English version
Here is the link to the French version.
Concerning the validation of the SOP to inactivate GMOs with bleach, on nematode worms.
Part one of a validation experiment
Part two of a validation experiment
In the image on the left, we can see the treated Petri dishes, 3 days after the treatments.
Without bleach, the numerous 'FXKI' strain nematodes have eaten all their food (top right), and although the bacterial layer is still clearly visible for the TR1CB strain, without bleach there are many worms.
After treatments with 10% or 100% bleach, only the 10% TR1CB still had live nematodes.
An important message to take away from the validation: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used according to the SOP on culture plates kills all stages of C. elegans nematodes...
