Difference between revisions of "OpenInsulin"
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Revision as of 14:03, 7 February 2021
The Open Insulin project is part of an international collaboration.
At Hackuarium, the first results are merely: seq confirmation of plasmids from Alex Kelly
Winsulin and ProInsulin
to produce and test (+ controls)
The project
test
part1
Our goals
- Pave the way for more OpenInsulin tests with International Colleagues
- Demystify and explain the best practices toward any open science project
- Produce Winsulin and ProInsulin for open access positive controls
Why participatory research?
the means
Why Open Data?
By making all the data generated by the project open, we want to explore and promote an alternative to proprietary initiatives. Open data supports literacy and fosters innovation by both citizens and scholars. Open designs can still be produced for commercial ventures, and can make profits if there is a market for the product.
What are the specific challenges of the project and has anyone done this before?
Open Insulin history Hackuarium's Open Insulin Entanglements Alex Kelly to Geneva Plasmids finally to Hackuarium, around August 2020, for Winsulin and ProInsulin, both constructs based on periplasmic expression vectors.
Current status
Clones grown and finally sequence confirmation has come through.
Complications from low copy number vector and GC-rich stretches overcome via midipreps and 'power read upgrades' for the sequencing.
Looks great...
gel with HindIII diagnostic result.
and mini vs midipreps
seq traces are clean for (lengths)