Worm validations - Revision history

Rachel at 15:44, 27 November 2023

2023-11-27T15:44:39Z

2023-11-27T15:29:20Z

2023-11-27T05:31:55Z

2023-11-27T05:21:49Z

2023-11-27T05:14:26Z

2023-11-27T04:58:50Z

2023-11-27T04:57:53Z

2023-11-27T02:31:49Z

2023-11-26T20:40:47Z

2023-11-26T20:40:05Z

← Older revision		Revision as of 15:44, 27 November 2023
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	The liquid containing the worms was distributed to three individual tubes and treated under three conditions: just in water, in 10% bleach or in 100% bleach.		The liquid containing the worms was distributed to three individual tubes and treated under three conditions: just in water, in 10% bleach or in 100% bleach.
		+
		+	[[File:Comparison.jpg\|thumb\|validation tests in the P1 lab]]
		+	The image to the right shows one part of the experiment.

	[[File:Plate_imagep3.png\|thumb\|3 days after treatment\|alt=\|left]]		[[File:Plate_imagep3.png\|thumb\|3 days after treatment\|alt=\|left]]

← Older revision		Revision as of 15:29, 27 November 2023
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	After treatments with 10% or 100% bleach, only one plate, with the strain TR1CB, treated with 10% bleach, still had a few live nematodes.		After treatments with 10% or 100% bleach, only one plate, with the strain TR1CB, treated with 10% bleach, still had a few live nematodes.

		+	All plates were inactivated according to the SOP after the third day of observation, as usual, with undiluted bleach (2.5% hypochlorite).

		+	****

−	An important message to take away from the validation: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing according to the SOP] on culture plates kills all stages of ''C. elegans'' nematodes...~~[[File:Postvalid nematodes.png\|thumb\|disinfection after the third day of one set of post-validation tests..]]~~	+	An important message to take away from the worm validation tests: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing according to the SOP] on culture plates kills all stages of ''C. elegans'' nematodes...

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 This validation is part of our [[LaboP1 EN|updates and revisions of the lab biosafety procedures]].
+Here is the similar [[Validated GMO inactivation|validation for the  case of GMO bacteria]].
 <nowiki>*</nowiki>
 To note:  The titles of many our wiki pages are not always perfect, but the content can be improved by everyone, too.  This is the idea of the open science approach - to 'Do-It-Together'.  =)

← Older revision	Revision as of 05:21, 27 November 2023
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−	+	This validation is part of our [[LaboP1 EN\|updates and revisions of the lab biosafety procedures]].

@@ Line 9: / Line 9: @@
 [https://wiki.hackuarium.ch/images/5/50/Nematodes2_smaller.pdf Part two of a validation experiment]
-For this experiment, initial worm cultures of two different GMO worm strains, both carrying a marker gene that makes the worms 'Roll' (actually a mutated collagen gene, termed ''rol-6'', on a plasmid with an antibiotic resistance marker) were grown to starvation for one week, and then washed off plates with water.
+For [https://www.evernote.com/shard/s586/sh/4ff409d3-1754-4d1f-8507-ea7780f1b1c4/cwK7hplOCDHfUdnjJhScKxOfGgGzVJDRz9nDTJY1vrBHYZkT2atVzeDnFQ this experiment], initial worm cultures of two different GMO worm strains, both carrying a marker gene that makes the worms 'Roll' (actually a mutated collagen gene, termed ''rol-6'', on a plasmid with an antibiotic resistance marker) were grown to starvation for one week, and then washed off plates with water.
 Here is a [https://drive.google.com/file/d/1LalApnyA56MxREW_ELxDNaYdRltnH4zB/view?usp=sharing 3-second 'live' video of 'Roller' worms] from one of these starved plates, before being washed.

@@ Line 13: / Line 13: @@
 Here is a [https://drive.google.com/file/d/1LalApnyA56MxREW_ELxDNaYdRltnH4zB/view?usp=sharing 3-second 'live' video of 'Roller' worms] from one of these starved plates, before being washed.
-The liquid containing the worms was distributed to three individual tubes and treated under three conditions: left in the water with
+The liquid containing the worms was distributed to three individual tubes and treated under three conditions: just in water, in 10% bleach or in 100% bleach.
 [[File:Plate_imagep3.png|thumb|3 days after treatment|alt=|left]]

@@ Line 3: / Line 3: @@
 Here is the [[Validations nematodes|link to the French version]].
-Concerning the validation of the SOP to inactivate GMOs with bleach, on nematode worms.
+This page is a simplified summary, concerning the validation of the [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing standard operating procedure (SOP) used to inactivate genetically modified organisms (GMOs) with bleach], for use on nematode worms.
 [https://wiki.hackuarium.ch/images/5/56/Nematodes1_smaller.pdf Part one of a validation experiment]
 [https://wiki.hackuarium.ch/images/5/50/Nematodes2_smaller.pdf Part two of a validation experiment]
 [[File:Plate_imagep3.png|thumb|3 days after treatment|alt=|left]]
-In the image on the left, we can see the treated Petri dishes, 3 days after the treatments.
+In the image on the left are shown the secondary worm plates after treatments, 3 days after the treatments.
 Without bleach, the numerous 'FXKI' strain nematodes have eaten all their food (top right), and although the bacterial layer is still clearly visible for the TR1CB strain, without bleach there are many worms.

← Older revision		Revision as of 20:40, 26 November 2023
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	An important message to take away from the validation: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing according to the SOP] on culture plates kills all stages of ''C. elegans'' nematodes...[[File:Postvalid nematodes.png\|thumb\|disinfection after the third day of one set of post-validation tests..]]		An important message to take away from the validation: resistant larvae (in the 'dauer' stage) can survive 10% bleach, but 100% bleach used [https://drive.google.com/file/d/1_db3H7Ybm_kd7yV-wnMKF55Snr805TAp/view?usp=sharing according to the SOP] on culture plates kills all stages of ''C. elegans'' nematodes...[[File:Postvalid nematodes.png\|thumb\|disinfection after the third day of one set of post-validation tests..]]
		+
		+	<nowiki>*</nowiki>
		+	The titles of many our wiki pages are not always perfect, but the content can be improved by everyone, too. This is the idea of the open science approach - to 'Do-It-Together'. =)