Urban gardens with rhizobial bacteria - Revision history

Rachel at 15:49, 11 March 2026

2026-03-11T15:49:26Z

Rachel at 15:07, 3 June 2025

2025-06-03T15:07:31Z

Rachel at 11:17, 17 April 2024

2024-04-17T11:17:51Z

Rachel at 17:55, 28 March 2023

2023-03-28T17:55:35Z

Rachel at 18:04, 24 April 2022

2022-04-24T18:04:08Z

Rachel at 18:03, 24 April 2022

2022-04-24T18:03:35Z

Rachel at 15:11, 26 March 2022

2022-03-26T15:11:09Z

Rachel at 15:09, 26 March 2022

2022-03-26T15:09:11Z

Rachel at 15:08, 26 March 2022

2022-03-26T15:08:38Z

Rachel at 15:06, 26 March 2022

2022-03-26T15:06:34Z

@@ Line 13: / Line 13: @@
 However, the fourth run in 2021 was disappointing, with weather contributing to less results than expected and no chance for stat significance... <br>
 <br>
-UPDATE: In 2024, hopes to try again have been high, with a gardening group ('Les P'tites Pousses' of Echandens) also keen... until we found out that the rules for shipping the bacteria had changed!  A -80 freezer is needed for our lab!  Nothing really has happened post-pandemic... Even the fifth trial for 2022 was mainly controls - with a field in Echandens to follow - from treated seed already sown before Christmas 2021.
+Because we had no bacteria growing in 2024 we decided to do a special control with the P'tites pousses... (and in Michel's garden) as Rachel went for a sabbatical to Seattle for 6 months.
-For the experiment to continue again, more involved members are necessary. Maybe this is for you?
+'''Then, this page goes back in time again.  We will need to make a new page for [[2026 Urban Gardens]].'''
-<br>
+We actually only had two sign-ups confirmed as of the date of the General Assembly meeting in February 2022, to determine whether another experiment would be initiated.  New media to grow the symbiotic culture was already tested, after a failure to get growth, even from the -80 gly stock at the UNIL DMF.  In fact 3 attempts were made, and it was not a success. The peat moss was just not a good substitute for the African violet soil called for in the media!
-However, as there is a big pea field in Echandens, which is already being followed closely in 2022, we decided to make it a special control year. The field planted already before the end of 2021, with [https://www.farmkidblog.com/2016/04/20/why-are-your-seeds-pink/ bright pink seeds, which could mean] that they were treated with rhizobial cultures (or a fungicide)!  We will try to find out more!
+However, as there was a big pea field in Echandens, which was already being followed closely in 2022, we decided to make it a special control year. The field planted already before the end of 2021, with [https://www.farmkidblog.com/2016/04/20/why-are-your-seeds-pink/ bright pink seeds, which could mean] that they were treated with rhizobial cultures (or a fungicide.  We were not successful to find out more!)
 Such good growth as shown to right with no special care, is worth noting.
-In lab we are germinating seeds, and people will be able to get A and B sets again, and we will see how the numbers come out!  Could be interesting!
+In lab we again germinated seeds, and people got A and B sets again.  But
 We hope to start distributing plants maybe even next Weds (but probably the week after)...
@@ Line 69: / Line 74: @@
 Watch this space! <br>
-More will come soon, and new pages... '''TRANSLATORS REQUESTED!''' ( all the below is being left for historical purposes)<br>
+More will come soon, and new pages... <br>
 <br>

← Older revision		Revision as of 15:07, 3 June 2025
Line 1:		Line 1:

−	(à la [http://wiki.hackuarium.ch/w/Jardinage_urbain version française] - merci, Maurice!	+	(à la [http://wiki.hackuarium.ch/w/Jardinage_urbain version française] - merci, Maurice! also, still need to add info for 2023 & 2024 esp as the extra magnesium in the latter year was worse for the harvests... for 2025, only home gardening is being done, and slugs ate a lot to start, but by June there are still plants recuperating...)

	Up to March 2022, four annual trials for a project focussed on '''urban gardens with rhizobial bacteria''' have tested whether specific rhizobial bacteria will improve the growth of peas when used by ordinary people. <br>		Up to March 2022, four annual trials for a project focussed on '''urban gardens with rhizobial bacteria''' have tested whether specific rhizobial bacteria will improve the growth of peas when used by ordinary people. <br>

@@ Line 2: / Line 2: @@
 (à la [http://wiki.hackuarium.ch/w/Jardinage_urbain version française] - merci, Maurice!
-To date (25.3.22) four annual trials for a project focussed on '''urban gardens with rhizobial bacteria''' have been testing whether specific rhizobial bacteria will improve the growth of peas when used by ordinary people. <br>
+Up to March 2022, four annual trials for a project focussed on '''urban gardens with rhizobial bacteria''' have tested whether specific rhizobial bacteria will improve the growth of peas when used by ordinary people. <br>
 To note: such cultures are normally used on the agro-business scale! <br>
-Here is [http://www.cilr.uq.edu.au/legume-information some basic info about such symbiosis for nitrogen utilisation] by plants.<br>
+Here is [http://www.cilr.uq.edu.au/legume-information some basic info about such symbiosis for nitrogen utilisation] by plants.
 [[File:IMG 9589.jpg|200px|thumb|left|a pea field in Echandens]] [[File:Pea1.jpg|thumb|Echandens pea field 2022]] [[File:Pea1 closeup26mar22.jpg|thumb|no watering for peas in field.]]
@@ Line 10: / Line 12: @@
 The rhizobial strain needed for peas, ''Rhizobium leguminosarum Jordan'', was ordered from ATCC the first year and from Carolina.com the following year, after the frozen stocks did not seem happy after our move to Ecublens.  It was grown with a special Rhizobium media, and used for controlled tests on symbiotic effects on pea harvests, comparing growth of inoculated and 'mock' inoculated pea plant sprouts. Our third trial was begun in the time of covid-19 and may have provided the best results to date. :)
-However, the fourth run in 2021 was disappointing, with weather contributing to less results than expected and no chance for stat sig... <br>
+However, the fourth run in 2021 was disappointing, with weather contributing to less results than expected and no chance for stat significance... <br>
 <br>
-UPDATE: '''The fifth trial for 2022 was mainly controls - with a field in Echandens to follow - from treated seed already sown before Christmas 2021. For the experiment to continue in 2023, more involved members are necessary. Maybe this is for you?'''
+UPDATE: In 2024, hopes to try again have been high, with a gardening group ('Les P'tites Pousses' of Echandens) also keen... until we found out that the rules for shipping the bacteria had changed!  A -80 freezer is needed for our lab!  Nothing really has happened post-pandemic... Even the fifth trial for 2022 was mainly controls - with a field in Echandens to follow - from treated seed already sown before Christmas 2021.
 <br>
-We only had two sign-ups confirmed as of the date of our General Assembly meeting last year (16feb22), to determine whether another experiment will be initiated.  New media to grow the symbiotic culture was already tested, after a failure to get growth, even from the -80 gly stock at the UNIL DMF.  In fact 3 attempts were made, and it was not a success. I need to get African violet soil again for the media! (the peat moss I tried this year did not make a happy culture...)
+We only had two sign-ups confirmed as of the date of the General Assembly meeting in February 2022), to determine whether another experiment will be initiated.  New media to grow the symbiotic culture was already tested, after a failure to get growth, even from the -80 gly stock at the UNIL DMF.  In fact 3 attempts were made, and it was not a success. I need to get African violet soil again for the media! (the peat moss I tried this year did not make a happy culture...)
-However, as there is a big pea field in Echandens, which is already being followed closely this year, we have decided to make this a special control year. The field planted already before the end of 2021, with [https://www.farmkidblog.com/2016/04/20/why-are-your-seeds-pink/ bright pink seeds, which could mean] that they were treated with rhizobial cultures (or a fungicide)!  We will try to find out more!
+However, as there is a big pea field in Echandens, which is already being followed closely in 2022, we decided to make it a special control year. The field planted already before the end of 2021, with [https://www.farmkidblog.com/2016/04/20/why-are-your-seeds-pink/ bright pink seeds, which could mean] that they were treated with rhizobial cultures (or a fungicide)!  We will try to find out more!
 Such good growth as shown to right with no special care, is worth noting.

@@ Line 12: / Line 12: @@
 However, the fourth run in 2021 was disappointing, with weather contributing to less results than expected and no chance for stat sig... <br>
 <br>
-UPDATE: '''The fifth trial for 2022 is going to be a special control year.'''<br>
+UPDATE: '''The fifth trial for 2022 was mainly controls - with a field in Echandens to follow - from treated seed already sown before Christmas 2021. For the experiment to continue in 2023, more involved members are necessary. Maybe this is for you?'''
 We only had two sign-ups confirmed as of the date of our General Assembly meeting (16feb22), to determine whether another experiment will be initiated.  New media to grow the symbiotic culture was already tested, after a failure to get growth, even from the -80 gly stock at the UNIL DMF.  In fact 3 attempts were made, and it was not a success. I need to get African violet soil again for the media! (the peat moss I tried this year did not make a happy culture...)
 However, as there is a big pea field in Echandens, which is already being followed closely this year, we have decided to make this a special control year. The field planted already before the end of 2021, with [https://www.farmkidblog.com/2016/04/20/why-are-your-seeds-pink/ bright pink seeds, which could mean] that they were treated with rhizobial cultures (or a fungicide)!  We will try to find out more!

@@ Line 27: / Line 27: @@
 the fourth trial was unusual, with late snow and some striking but overall non-statistically significant results.''' <br>There were records broken, nonetheless, especially with 10 peas per pod! AND still peas to harvest (and some new flowers) in July 2021.  An overall summary was again presented during the Fête de la Science et Biodiversité 2021 in Ferney-Voltaire FR. <br>=)<br>'''
 Briefly, for the 2021 results, in addition, one hypothesis from the previous year about hydroponic conditions was refuted.  We got better yields with 'fed' plants in seramis clay bits... The bacterial symbiosis really seemed to improve yields in 2021, in pots, especially, the conditions with rhizobial bacteria gave better harvests. However, too many plants died from the late frost for any real statistical confidence overall. Furthermore, in some gardens the plants that survived seemed to do even better without the rhizobial bacteria!
 If you are interested please contact us (hello -at- hackuarium -dot- ch)!<br>Your interest will encourage us to do more!

@@ Line 21: / Line 21: @@
 <br>It must also be acknowledged:
-the fourth trial was unusual, with late snow and some striking but overall non-statistically significant results.''' <br>There were still peas to harvest (and some new flowers) in July 2021. nonetheless! and an overall summary was again presented during the Fête de la Science et Biodiversité 2021 in Ferney-Voltaire FR. <br>=)<br>'''
+the fourth trial was unusual, with late snow and some striking but overall non-statistically significant results.''' <br>There were records broken, nonetheless, especially with 10 peas per pod! AND still peas to harvest (and some new flowers) in July 2021.  An overall summary was again presented during the Fête de la Science et Biodiversité 2021 in Ferney-Voltaire FR. <br>=)<br>'''
-Briefly, one hypothesis from the previous year about hydroponic conditions was refuted - we got better yields with 'fed' plants in seramis clay bits... The bacterial symbiosis really seemed to improve yields in 2021, in pots, especially, the conditions with rhizobial bacteria gave better harvests. However, too many plants died from the late frost for any real statistical confidence overall. Furthermore, in some gardens the plants that survived seemed to do even better without the rhizobial bacteria!
+Briefly, for the 2021 results, in addition, one hypothesis from the previous year about hydroponic conditions was refuted.  We got better yields with 'fed' plants in seramis clay bits... The bacterial symbiosis really seemed to improve yields in 2021, in pots, especially, the conditions with rhizobial bacteria gave better harvests. However, too many plants died from the late frost for any real statistical confidence overall. Furthermore, in some gardens the plants that survived seemed to do even better without the rhizobial bacteria!
 Thus, after 4 trials, we still have not decided whether to do the 5th!
-If you are interested please contact us (hello -at- hackuarium -dot- ch)!<br>
+If you are interested please contact us (hello -at- hackuarium -dot- ch)!<br>Your interest will encourage us to do more!

@@ Line 15: / Line 15: @@
 We only had two sign-ups confirmed as of the date of our General Assembly meeting (16feb22), to determine whether another experiment will be initiated.  New media to grow the symbiotic culture is currently being tested, after a failure to get growth, even from the -80 gly stock at the UNIL DMF.
-However, whatever happens, there is a big pea field in Echandens that is being followed closely this year - planted before the end of 2021, with [https://www.farmkidblog.com/2016/04/20/why-are-your-seeds-pink/ bright pink seeds, which could mean] that they were treated with rhizobial cultures (or a fungicide)!  We will try to find out more!
+However, whatever happens, there is a big pea field in Echandens that is being followed closely this year - planted already before the end of 2021, with [https://www.farmkidblog.com/2016/04/20/why-are-your-seeds-pink/ bright pink seeds, which could mean] that they were treated with rhizobial cultures (or a fungicide)!  We will try to find out more!
 Such good growth as shown to right with no special care, is worth noting.