P1 Lab Activities - Revision history

Rachel: /* Further information */

2025-02-16T18:37:26Z

Further information

Rachel: /* SOPs and Validation Experiments */

2023-12-21T10:31:35Z

SOPs and Validation Experiments

Rachel: /* History and Biosafety Officers */

2023-12-21T10:28:43Z

History and Biosafety Officers

Rachel: /* SOPs and Validation Experiments */

2023-12-21T10:16:28Z

SOPs and Validation Experiments

Rachel: /* Further information */

2023-12-15T22:27:35Z

Further information

Rachel: /* SOPs and Validation Experiments */

2023-12-15T22:24:34Z

SOPs and Validation Experiments

Rachel: /* SOPs and Validation Experiments */

2023-12-15T22:19:55Z

SOPs and Validation Experiments

Rachel at 14:54, 20 November 2023

2023-11-20T14:54:50Z

Rachel at 15:49, 13 November 2023

2023-11-13T15:49:30Z

Rachel at 15:46, 13 November 2023

2023-11-13T15:46:23Z

@@ Line 173: / Line 173: @@
 Come join us!<br>
-* Do you have questions? Maybe you can't reach Rachel or Javier, or want to find out more before even trying to ask them??  (While there is still an active link to an old service in the DIYBio community for this - [http://ask.diybio.org/ Ask a Biosafety Professional] - this may no longer be a functioning entity - our 2nov23 test has not received any response...)
+* Do you have questions? Maybe you can't reach Rachel or Javier, or want to find out more before even trying to ask them??  (maybe the old advice site can be found resurrected)
 * The [https://communitybio.pubpub.org/pub/m255th2q/release/1 community lab biosafety handbook] has very extensive details, thanks to colleagues around the world.<br>
 <br>
   '''Remember, being clueless is no excuse!!'''
 [[Category:Work In Progress]]

@@ Line 103: / Line 103: @@
 To summarise briefly, each set of experiments tested how many surviving GMOs would be found after inactivation tests with 100% and 10% bleach, or just water.  In both cases, bacteria or worms, the 10% solutions were sufficient to prevent further growth of the GMOs.  However, using the 100% solution is a bit more safe, as some contaminants, like spores from molds, might only be inactivated at higher concentrations.
-For details: please see [[Report ValidTestsGMOinact|the report]] and more [[Validated GMO inactivation|visual summary]], both also available in French versions ([[Rapport inactOGM|rapport]] & résumés sur [[OGMinact validation|bactéries]] et [[Validations nematodes|nématodes]]) and you can also [https://www.evernote.com/shard/s586/sh/9e3be51e-434c-6b7d-25ed-ecb34b5bfe2a/HNkV4W3AW3UMJNqd7--KWVclJ5uwYYFC3Xq7oAL2MPJkgITpTeUvG5wErw see Evernote with details for the bacterial] and  [https://www.evernote.com/shard/s586/sh/4ff409d3-1754-4d1f-8507-ea7780f1b1c4/cwK7hplOCDHfUdnjJhScKxOfGgGzVJDRz9nDTJY1vrBHYZkT2atVzeDnFQ for the transformed worms] tests.
+For details: please see [[Report ValidTestsGMOinact|the report]] and more [[Validated GMO inactivation|visual summary]], both also available in French versions ([[Rapport inactOGM|rapport]] & résumés sur les validations avec [[OGMinact validation|bactéries]] et [[Validations nematodes|nématodes]]) and you can also [https://www.evernote.com/shard/s586/sh/9e3be51e-434c-6b7d-25ed-ecb34b5bfe2a/HNkV4W3AW3UMJNqd7--KWVclJ5uwYYFC3Xq7oAL2MPJkgITpTeUvG5wErw see Evernote with details for the bacterial] and  [https://www.evernote.com/shard/s586/sh/4ff409d3-1754-4d1f-8507-ea7780f1b1c4/cwK7hplOCDHfUdnjJhScKxOfGgGzVJDRz9nDTJY1vrBHYZkT2atVzeDnFQ for the transformed worms] tests.
+*

@@ Line 34: / Line 34: @@
 Let us know if you want to do P1 experiments!<br>
 =History and Biosafety Officers=
 A P1 laboratory, allowing members to select for bacterial clones carrying plasmids they wanted to use or make, was officially functional at the old Hackuarium site in Renens between 1 November and 1 September 2018.
@@ Line 45: / Line 47: @@
 If you need to contact them in particular about any proposed or in progress P1 activities, this is the best email address: biolab@hackuarium.ch <br>
-More than a dozen people have already taken the "Introduction to biosafety" training necessary for full access to the P1 space. '''More training sessions can be organised upon request.'''
+Many people have already taken the "Introduction to biosafety" training necessary for full access to the P1 space. This has now been updated, and '''more training sessions can be organised upon request.'''
 Our P1 lab was inspected by representatives of the federal office for the environment in 2019 and in 2023, which probably have helped improve things greatly.
@@ Line 51: / Line 53: @@
 ''Notre laboratoire P1 était inspecté par les mandataires de l'office fédéral de l'environnement (OFEV) en 2019 et en 2023, qui ont probablement contribué à améliorer grandement les choses..''<br>
-This page (and others around biosafety and the P1 lab), probably still need more updates and many are still lacking most French translations. '''If you are interested in helping, please reach out!'''<br>
+<nowiki>*</nowiki>This page (and others around biosafety and the P1 lab), probably still need more updates and many are still lacking most French translations. '''If you are interested in helping, please reach out!'''<br>
 =P1 experiments at Hackuarium=

@@ Line 101: / Line 101: @@
 To summarise briefly, each set of experiments tested how many surviving GMOs would be found after inactivation tests with 100% and 10% bleach, or just water.  In both cases, bacteria or worms, the 10% solutions were sufficient to prevent further growth of the GMOs.  However, using the 100% solution is a bit more safe, as some contaminants, like spores from molds, might only be inactivated at higher concentrations.
-For details: please see [[Report ValidTestsGMOinact|the report]] and more [[Validated GMO inactivation|visual summary]], and you can also [https://www.evernote.com/shard/s586/sh/9e3be51e-434c-6b7d-25ed-ecb34b5bfe2a/HNkV4W3AW3UMJNqd7--KWVclJ5uwYYFC3Xq7oAL2MPJkgITpTeUvG5wErw see Evernote with details for the bacterial] and  [https://www.evernote.com/shard/s586/sh/4ff409d3-1754-4d1f-8507-ea7780f1b1c4/cwK7hplOCDHfUdnjJhScKxOfGgGzVJDRz9nDTJY1vrBHYZkT2atVzeDnFQ for the transformed worms] tests.
+For details: please see [[Report ValidTestsGMOinact|the report]] and more [[Validated GMO inactivation|visual summary]], both also available in French versions ([[Rapport inactOGM|rapport]] & résumés sur [[OGMinact validation|bactéries]] et [[Validations nematodes|nématodes]]) and you can also [https://www.evernote.com/shard/s586/sh/9e3be51e-434c-6b7d-25ed-ecb34b5bfe2a/HNkV4W3AW3UMJNqd7--KWVclJ5uwYYFC3Xq7oAL2MPJkgITpTeUvG5wErw see Evernote with details for the bacterial] and  [https://www.evernote.com/shard/s586/sh/4ff409d3-1754-4d1f-8507-ea7780f1b1c4/cwK7hplOCDHfUdnjJhScKxOfGgGzVJDRz9nDTJY1vrBHYZkT2atVzeDnFQ for the transformed worms] tests.
+*

@@ Line 165: / Line 165: @@
 * General Community Lab biosafety information is also [https://communitybio.pubpub.org/pub/m255th2q/release/1 available on line].
 * From our pandemic experiences, [https://docs.google.com/document/d/1ldQ1aRxHjuj-U7kNqwWlkwwwW54U6ls8xMI9l0LBFrQ/edit?usp=sharing here is another good starting point].
-*Here is the [https://drive.google.com/open?id=0B1jaPHOkH_42dVhjOXBBN0xsUms link to all the initial P1 safety info, including our list of users and list of strains] for future reference. <br>
+*Here is the [https://drive.google.com/open?id=0B1jaPHOkH_42dVhjOXBBN0xsUms link to all the initial P1 safety info, including our list of users and list of strains] for future reference.
 <br>
 See something that needs mentioning?  You can help contribute!

@@ Line 97: / Line 97: @@
 '''Standard Operation Procedure for inactivation of genetically modified organisms (GMOs) - bilingual FR/EN'''
-Formal validation of the above SOP for GMO inactivation for disposal has now been done using two kinds of commercial bleach on both fluorescent bacteria, and on nematodes transformed with the a plasmid, with good results.
+Formal validation of the above SOP for GMO inactivation for disposal has now been done using commercial bleach on both fluorescent bacteria, and on nematodes transformed with the a plasmid, with good results.
 To summarise briefly, each set of experiments tested how many surviving GMOs would be found after inactivation tests with 100% and 10% bleach, or just water.  In both cases, bacteria or worms, the 10% solutions were sufficient to prevent further growth of the GMOs.  However, using the 100% solution is a bit more safe, as some contaminants, like spores from molds, might only be inactivated at higher concentrations.

@@ Line 1: / Line 1: @@
 [[File:Hackuarium_logo15.png|200px|thumb|]]
-Our P1 lab in Ecublens is the place where Hackuarium members can do synthetic biology and more, depending on risk analyses and plans.  [https://drive.google.com/file/d/1tD2egvEsSemr7c4-DSdV0oi7O3rml2Ub/view?usp=sharing Here] are info for the current users, and your information...  More documentation of our activities can be found linked from this current page, and the [[P1 Biosec2|most recent around the P1]] work..
+Our P1 lab in Ecublens is the place where Hackuarium members can do synthetic biology and more, depending on risk analyses and plans.  [https://drive.google.com/file/d/1tD2egvEsSemr7c4-DSdV0oi7O3rml2Ub/view?usp=sharing Here] are info for the current users, and your information...  More documentation of our activities can be found linked from this current page, and the [[LaboP1 EN|most recent around the P1]] work..
 Of course, biosafety is a key interest, and all users abide by the [https://diybio.org/codes/code-of-ethics-north-america-congress-2011/ ethical code]* of our open science community lab.
-''Notre laboratoire P1 à Ecublens est l'endroit où les membres de Hackuarium peuvent faire de la biologie synthétique et plus, en fonction des analyses de risque et des plans. [https://drive.google.com/file/d/1tD2egvEsSemr7c4-DSdV0oi7O3rml2Ub/view?usp=sharing Voici des informations] pour les utilisateurs actuels, et vos informations... Plus de documentation sur nos activités peut être trouvée sur cette page, et la [[P1 Biosec2 (FR)|plus récente sur notre travail en laboratoire P1]].''
+''Notre laboratoire P1 à Ecublens est l'endroit où les membres de Hackuarium peuvent faire de la biologie synthétique et plus, en fonction des analyses de risque et des plans. [https://drive.google.com/file/d/1tD2egvEsSemr7c4-DSdV0oi7O3rml2Ub/view?usp=sharing Voici des informations] pour les utilisateurs actuels, et vos informations... Plus de documentation sur nos activités peut être trouvée sur cette page, et la [[LaboP1|plus récente sur notre travail en laboratoire P1]].''
 ''Bien sûr, la biosécurité est un intérêt majeur, et tous les utilisateurs respectent [https://diybio.org/codes/code-of-ethics-north-america-congress-2011/ le code éthique]* de notre laboratoire communautaire de science ouverte.''

← Older revision		Revision as of 15:46, 13 November 2023
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−	''Notre laboratoire P1 à Ecublens est l'endroit où les membres de Hackuarium peuvent faire de la biologie synthétique et plus, en fonction des analyses de risque et des plans. [https://~~docs~~.google.com/~~document~~/d/~~1cNfiyZB4YODJY~~-~~FnM8q2hJBs0yHHTcyanrDvVT4KvE8~~/~~edit~~?usp=sharing Voici des informations] pour les utilisateurs actuels, et vos informations... Plus de documentation sur nos activités peut être trouvée sur cette page.''	+	''Notre laboratoire P1 à Ecublens est l'endroit où les membres de Hackuarium peuvent faire de la biologie synthétique et plus, en fonction des analyses de risque et des plans. [https://drive.google.com/file/d/1tD2egvEsSemr7c4-DSdV0oi7O3rml2Ub/view?usp=sharing Voici des informations] pour les utilisateurs actuels, et vos informations... Plus de documentation sur nos activités peut être trouvée sur cette page.''

	''Bien sûr, la biosécurité est un intérêt majeur, et tous les utilisateurs respectent [https://diybio.org/codes/code-of-ethics-north-america-congress-2011/ le code éthique]* de notre laboratoire communautaire de science ouverte.''		''Bien sûr, la biosécurité est un intérêt majeur, et tous les utilisateurs respectent [https://diybio.org/codes/code-of-ethics-north-america-congress-2011/ le code éthique]* de notre laboratoire communautaire de science ouverte.''