Fun with fungi - Revision history

Rachel at 12:36, 27 May 2025

2025-05-27T12:36:10Z

Rachel: /* Gallery */

2025-05-13T16:04:09Z

Gallery

Rachel at 17:51, 2 April 2025

2025-04-02T17:51:55Z

Rachel: /* Fun With Fungi */

2025-03-12T18:06:46Z

Fun With Fungi

Rachel: /* Fun With Fungi */

2024-01-16T08:32:20Z

Fun With Fungi

Rachel at 08:46, 2 November 2023

2023-11-02T08:46:48Z

Rachel: /* Fun With Fungi */

2023-10-26T16:02:38Z

Fun With Fungi

Rachel at 16:02, 26 October 2023

2023-10-26T16:02:03Z

Rachel at 15:11, 26 October 2023

2023-10-26T15:11:07Z

Rachel at 15:04, 26 October 2023

2023-10-26T15:04:22Z

@@ Line 29: / Line 29: @@
 We hope to do more metagenomic analyses for participatory research on mycelial biodiversity, including the intriguing possibility that mycelia might help save the bees!
-Our latest [https://experiment.com/projects/participatory-research-to-explore-fungal-biodiversity-and-its-importance-to-bees crowdfunding campaign] launched on 8Feb23, and we hope it helps us do this seriously, to allow not only extraction of complex samples and MinION kits etc needed for the barcoding, as for our [[Open-Food-DNA|OpenFoodRepo DNA]] work... but also growth of mycelia on beehive inserts, to provide for bees, to test how they might help bees prosper, as [https://www.nature.com/articles/s41598-018-32194-8 previously published work by other mushroom experts] suggests.)
+A [https://experiment.com/projects/participatory-research-to-explore-fungal-biodiversity-and-its-importance-to-bees crowdfunding campaign] launched on 8Feb23, and it has helped us do this seriously, to allow not only extraction of complex samples and MinION kits etc needed for the barcoding, as for our [[Open-Food-DNA|OpenFoodRepo DNA]] work... but also growth of mycelia on beehive inserts, to provide for bees, to test how they might help bees prosper, as [https://www.nature.com/articles/s41598-018-32194-8 previously published work by other mushroom experts] suggests.)
 Happily, the campaign reached its budget goal, and our Kickoff Workshop was held on Earth Day, 22 April 23 from 14-17h!
@@ Line 60: / Line 60: @@
 Around this project we have also helped launch a new community biolab, Thyrea Biofablab, with a [[fungal-themed biofest]].  Additionally, we have some [[GrowthPatterns|results on their growth patterns]] in petri dishes...  Light indeed, as suspected, makes a difference!
-We are also going to see if the growth on cigarette butts, to detoxify them, can be replicated in our lab. Here is [https://www.sciencedirect.com/science/article/pii/S2405844021002498 an academic paper around this idea]. Our first culture has already been made (butts collected outside and sterilised in our pressure cooker), comparing oyster mushroom growth on the cigarette butts to growth on sterilised moss. (added one picture to the gallery, so far, late october 2023) but it wasn't soo impressive in the end. the cigarette butts did not disappear.
+We are also going to see if the growth on cigarette butts, to detoxify them, can be replicated in our lab. Here is [https://www.sciencedirect.com/science/article/pii/S2405844021002498 an academic paper around this idea]. Our first culture has already been made (butts collected outside and sterilised in our pressure cooker), comparing oyster mushroom growth on the cigarette butts to growth on sterilised moss. (added one picture to the gallery, so far, late october 2023) but it wasn't soo impressive in the end, as the cigarette butts did not disappear.  However, we recently found another similar [https://www.purifungi.com/ belgian project, making ashtrays specifically], which says it's process is patented but also failed to get rid of the old butts...  Yngvar suggests using a brown rot mushroom instead of the pleurotes, and let's see.

@@ Line 75: / Line 75: @@
 File:PleurotesOnMegots.jpeg|pleurotes growing on cigarette butts (16days post inoculation)
 File:Workshopbowls.JPG|'fruit bowls' from another workshop getting a bit more growth
-File:Myceliacontam.jpg| mycelia d'un contaminant des nématodes.  belle! =)
+File:Myceliacontam.jpg|mycelia d'un contaminant des nématodes.  belle! =)
 </gallery>
 [[Category:In Progress]]

@@ Line 75: / Line 75: @@
 File:PleurotesOnMegots.jpeg|pleurotes growing on cigarette butts (16days post inoculation)
 File:Workshopbowls.JPG|'fruit bowls' from another workshop getting a bit more growth
 </gallery>
 [[Category:In Progress]]

@@ Line 60: / Line 60: @@
 Around this project we have also helped launch a new community biolab, Thyrea Biofablab, with a [[fungal-themed biofest]].  Additionally, we have some [[GrowthPatterns|results on their growth patterns]] in petri dishes...  Light indeed, as suspected, makes a difference!
-We are also going to see if the growth on cigarette butts, to detoxify them, can be replicated in our lab. Here is [https://www.sciencedirect.com/science/article/pii/S2405844021002498 an academic paper around this idea]. Our first culture has already been made (butts collected outside and sterilised in our pressure cooker), comparing oyster mushroom growth on the cigarette butts to growth on sterilised moss. (added one picture to the gallery, so far, late october 2023)
+We are also going to see if the growth on cigarette butts, to detoxify them, can be replicated in our lab. Here is [https://www.sciencedirect.com/science/article/pii/S2405844021002498 an academic paper around this idea]. Our first culture has already been made (butts collected outside and sterilised in our pressure cooker), comparing oyster mushroom growth on the cigarette butts to growth on sterilised moss. (added one picture to the gallery, so far, late october 2023) but it wasn't soo impressive in the end. the cigarette butts did not disappear.
 You can get involved, too!

@@ Line 73: / Line 73: @@
 File:Labels.jpg|P is for pupe, B is for black, W is for white...
 File:Ascosphaera apis 2e-174.png|sequence trace is quickly weak, but still highly significant... (2e-174!)
 File:PleurotesOnMegots.jpeg|pleurotes growing on cigarette butts (16days post inoculation)
 </gallery>
 [[Category:In Progress]]

@@ Line 56: / Line 56: @@
 '''Further Fun!'''
-Mycelia are also great [https://biosummit.live/bio-creation-station-3/hackuarium inspiration for bioart] works and [https://sustainablebrands.com/read/product-service-design-innovation/from-carbon-sinks-to-carbon-banks-could-mycelium-help-resolve-our-climate-crisis biostructural] and food [https://www.ecovative.com/ ideas] to help reduce carbon footprints...
+Mycelia are also great [https://biosummit.live/bio-creation-station-3/hackuarium inspiration for bioart] works and [https://sustainablebrands.com/read/product-service-design-innovation/from-carbon-sinks-to-carbon-banks-could-mycelium-help-resolve-our-climate-crisis biostructural] and food [https://www.ecovative.com/ ideas] to help reduce carbon footprints...  [[Biostructure2023|Here is a new page]] to collect recent biostructure work and information.
 Around this project we have also helped launch a new community biolab, Thyrea Biofablab, with a [[fungal-themed biofest]].  Additionally, we have some [[GrowthPatterns|results on their growth patterns]] in petri dishes...  Light indeed, as suspected, makes a difference!

@@ Line 31: / Line 31: @@
 Our latest [https://experiment.com/projects/participatory-research-to-explore-fungal-biodiversity-and-its-importance-to-bees crowdfunding campaign] launched on 8Feb23, and we hope it helps us do this seriously, to allow not only extraction of complex samples and MinION kits etc needed for the barcoding, as for our [[Open-Food-DNA|OpenFoodRepo DNA]] work... but also growth of mycelia on beehive inserts, to provide for bees, to test how they might help bees prosper, as [https://www.nature.com/articles/s41598-018-32194-8 previously published work by other mushroom experts] suggests.)
-Happily, the campaign reached its budget goal, and our Kickoff Workshop will be held on Earth Day, 22 April 23 from 14-17h!
+Happily, the campaign reached its budget goal, and our Kickoff Workshop was held on Earth Day, 22 April 23 from 14-17h!
 [[File:KimH translation.png|thumb|French text translated from the #mycelia4bees campaign.]]
 Here is a French Translation of the main text from the campaign, which a friend shared... =)
@@ Line 48: / Line 48: @@
 '''Special news:'''
-* For the Swiss Mycologists 'Wissenschaftliche Kommission' - we did mushroom DNA preparation and mITS1/4 pcr on 2023.10.03 for seqs.
+* For the Swiss Mycologists 'Wissenschaftliche Kommission' - we did mushroom DNA preparation and mITS1/4 pcr on 2023.10.03 for seqs. From 10ul reactions, we had enough DNA for results from 8 of 8 of the samples run, confirming identifications of the group.
-A Swiss beekeeper with problems around another microbe, ''Ascospaera,'' that mummifies larval worker bees, causing the serious problem 'couvain calcifié' (in french), will be sending us some samples, and RA did some molbio and got the expected species sequence of it as, ''Ascosphaera apis'' (one seq trace is shown in the Gallery below...), while Olivier was thinking about some competition experiments of it and ''Fomes fomentarius...''
+Another bit of news: A Swiss beekeeper with problems around a problematic microbe, ''Ascospaera,'' that mummifies larval worker bees, causing the serious problem 'couvain calcifié' (in french), had sent us some samples, and RA did some molbio and got the expected species sequence of it as, ''Ascosphaera apis'' (one seq trace is shown in the Gallery below...), while Olivier was thinking about some competition experiments of it and ''Fomes fomentarius...''
 See Gallery below.
 '''Further Fun!'''
@@ Line 60: / Line 58: @@
 Mycelia are also great [https://biosummit.live/bio-creation-station-3/hackuarium inspiration for bioart] works and [https://sustainablebrands.com/read/product-service-design-innovation/from-carbon-sinks-to-carbon-banks-could-mycelium-help-resolve-our-climate-crisis biostructural] and food [https://www.ecovative.com/ ideas] to help reduce carbon footprints...
-Around this project we have also helped launch a new community biolab, Thyrea Biofablab, with a [[fungal-themed biofest]].  Additionally, We have some [[GrowthPatterns|new results on their growth patterns]] in petri dishes...  Light indeed, as suspected, makes a difference!
+Around this project we have also helped launch a new community biolab, Thyrea Biofablab, with a [[fungal-themed biofest]].  Additionally, we have some [[GrowthPatterns|results on their growth patterns]] in petri dishes...  Light indeed, as suspected, makes a difference!
 We are also going to see if the growth on cigarette butts, to detoxify them, can be replicated in our lab. Here is [https://www.sciencedirect.com/science/article/pii/S2405844021002498 an academic paper around this idea]. Our first culture has already been made (butts collected outside and sterilised in our pressure cooker), comparing oyster mushroom growth on the cigarette butts to growth on sterilised moss. (to add pictures, late october 2023)

@@ Line 69: / Line 69: @@
 === Gallery ===
 <gallery mode="packed" widths="200" heights="200">
-File:Aesc_mycelia_transfers.jpg|thumb|new plates made from first
+File:Aesc mycelia transfers.jpg|new plates made from first
-File:Mycelia_tubes_for_extraction.jpg|thumb|mycelia collected
+File:Mycelia tubes for extraction.jpg|mycelia collected
-File:PupePCR.jpg|thumb|Three mITS1/4 bands cut out for purification and sequencing
+File:PupePCR.jpg|Three mITS1/4 bands cut out for purification and sequencing
-File:Fromtop.jpg|thumb|Growth on new plates
+File:Fromtop.jpg|Growth on new plates
-File:Labels.jpg|thumb|P is for pupe, B is for black, W is for white...
+File:Labels.jpg|P is for pupe, B is for black, W is for white...
+File:Ascosphaera apis 2e-174.png|sequence trace is quickly weak, but still highly significant... (2e-174!)
 </gallery>
 [[Category:In Progress]]

@@ Line 37: / Line 37: @@
 This is how we put it in [https://www.facebook.com/events/216763237659445 the fb event]:
-''(EN below) Atelier de biomatériaux avec mycéliums.''
+''(EN below)''
-''Nous avons prévu de passer en revue les différentes façons de cultiver les champignons, et les participants vont repartir avec un objet spécial qu'ils ont fabriqué. <br>
+''Atelier de biomatériaux avec mycéliums.''
-Biomaterials workshop with mycelia. **''
 ''We plan to review different ways to grow mushrooms, and participants will leave with a special object they have created!''
@@ Line 46: / Line 48: @@
 '''Special news:'''
-* We will be expecting Swiss Mycologists 'Wissenschaftliche Kommission' for DNA preparation and mITS1/4 pcr on 2023.10.03 and 4.
+* For the Swiss Mycologists 'Wissenschaftliche Kommission' - we did mushroom DNA preparation and mITS1/4 pcr on 2023.10.03 for seqs.
-A Swiss beekeeper with problems around another microbe, ''Ascospaera,'' that mummifies larval worker bees, causing the serious problem 'couvain calcifié' (in french), will be sending us some samples, and RA did some molbio and got the expected species sequence (can add more details), while Olivier was thinking about some competition experiments of it and ''Fomes fomentarius...''
+A Swiss beekeeper with problems around another microbe, ''Ascospaera,'' that mummifies larval worker bees, causing the serious problem 'couvain calcifié' (in french), will be sending us some samples, and RA did some molbio and got the expected species sequence of it as, ''Ascosphaera apis'' (one seq trace is shown in the Gallery below...), while Olivier was thinking about some competition experiments of it and ''Fomes fomentarius...''
 See Gallery below.