Biolab - Revision history

Rachel at 15:43, 29 May 2024

2024-05-29T15:43:47Z

Rachel: /* Further information */

2024-05-29T11:19:52Z

Further information

Rachel at 11:19, 29 May 2024

2024-05-29T11:19:30Z

Rachel at 11:18, 29 May 2024

2024-05-29T11:18:10Z

Rachel: /* Further information */

2023-11-05T16:46:38Z

Further information

Rachel: /* Further information */

2023-11-05T16:46:05Z

Further information

Rachel at 13:39, 3 November 2023

2023-11-03T13:39:12Z

Rachel at 13:38, 3 November 2023

2023-11-03T13:38:24Z

Rachel at 13:37, 3 November 2023

2023-11-03T13:37:12Z

Rachel: /* Biosafety Officers */

2023-11-03T13:33:49Z

Biosafety Officers

@@ Line 1: / Line 1: @@
 [[File:Hackuarium_logo15.png|100px|thumb]]
 '''Welcome to a page with information about the Hackuarium Biolab Space in Ecublens!'''
-''[[P1 Biosec2 (FR)|Pour FR et les infos plus recentes voir ici, svp]]''<br>
+[[LaboP1 EN|This wiki page gives more recent information in English.]]
 Hackuarium has two rooms in the context of the new cooperative, the Biolab with windows, and the so-called 'inner sanctum' without windows.
 The latter can be used for some biology work, especially higher resolution microscopy (with Raspberry Pi camera on the old Zeiss scope) and bioluminescence studies, but no growth of cultures is allowed there.

@@ Line 45: / Line 45: @@
 * All projects will have a box for their special equipment and stocks. Ask for a new one, if you need it!
 * Always tidy things away after manipulations are complete, and if anything needs to stay out on a bench, or in incubators, remember to always '''leave signed and dated notes'''!
-* Do you have questions? <br>Maybe you can't reach Rachel or Javier, or want to find out more before even trying to ask them?? <br> There is a service in the DIYBio community for this [http://ask.diybio.org/ Ask a Biosafety Professional]!  Use it! (actually in the midst of a double check to see if it still works -nov23)<br>
+* Do you have questions? <br>Maybe you can't reach Rachel or Aurélien, or want to find out more before even trying to ask them?? <br> There is a service in the DIYBio community for this [http://ask.diybio.org/ Ask a Biosafety Professional]!  Use it! (actually in the midst of a double check to see if it still works -nov23)<br>
 * '''Remember, being clueless is no excuse!!  there are many ways to find information.'''
 * The Standard Operating Procedures for appropriate use of the new space are being developed, and build upon what was [http://wiki.hackuarium.ch/w/P1_SOP previously decided during preparations to establish a P1 laboratory for Hackuarium members] already back in 2015/6. ([http://wiki.hackuarium.ch/w/Instru_Main Some old equipment] still has useful QR codes that lead to information in this wiki, too! Bravo, les poulpes!)

@@ Line 1: / Line 1: @@
 [[File:Hackuarium_logo15.png|100px|thumb]]
-'''Welcome to a page with information about the Hackuarium Biolab Space in Ecublens!''' <br>
+'''Welcome to a page with information about the Hackuarium Biolab Space in Ecublens!'''
 Hackuarium has two rooms in the context of the new cooperative, the Biolab with windows, and the so-called 'inner sanctum' without windows.
 The latter can be used for some biology work, especially higher resolution microscopy (with Raspberry Pi camera on the old Zeiss scope) and bioluminescence studies, but no growth of cultures is allowed there.

@@ Line 1: / Line 1: @@
 [[File:Hackuarium_logo15.png|100px|thumb]]
-'''Welcome to the page with information about the Hackuarium Biolab Space in Ecublens!''' <br>
+'''Welcome to a page with information about the Hackuarium Biolab Space in Ecublens!''' <br>
 Hackuarium has two rooms in the context of the new cooperative, the Biolab with windows, and the so-called 'inner sanctum' without windows.
 The latter can be used for some biology work, especially higher resolution microscopy (with Raspberry Pi camera on the old Zeiss scope) and bioluminescence studies, but no growth of cultures is allowed there.
@@ Line 15: / Line 15: @@
 =Biosafety Officers=
-Rachel Aronoff and Olivier Emery continued their roles as biosafety officers in the new space, until Olivier got too busy and left the board in June 2020, after which Javier Ortiz became the deputy.  Maybe '''you''' would like to become a second deputy biosafety officier? :)  <br>
+Rachel Aronoff and Olivier Emery continued their roles as biosafety officers in the new space, until Olivier got too busy and left the board in June 2020, after which Javier Ortiz became the deputy.  In 2024, Aurélien Jobard became deputy in his place.  Maybe '''you''' would like to become a second deputy biosafety officier? :)  <br>
 If you need to contact them in particular about any proposed activities or projects in the biolab, please use this email address: biolab@hackuarium.ch <br>
@@ Line 23: / Line 23: @@
 '''To learn, have fun, and avoid problems!''' <br>
-Here is the [https://drive.google.com/open?id=0B1jaPHOkH_42dVhjOXBBN0xsUms link to all the Biosafety info, list of users and list of strains to date] for future reference. <br>
+Here is the [https://drive.google.com/open?id=0B1jaPHOkH_42dVhjOXBBN0xsUms link to much initial Hackuarium Biosafety info, list of users and list of strains] . <br>
 After RA's first Biosummit in 2020, [https://docs.google.com/document/d/1Qkc2uCAcLX45b0GjSGZohweelJ-vDOhX5MDSf6F4MEI/edit?usp=sharing this great resource for community biolabs] came to her attention, for everyone's use. <br>
-We have to note that at least some lab strains did not survive the moving process, even though dry ice was used to move tubes from one -20 to another... :( <br>
+We have to note that at least some lab strains did not survive the moving process, even though dry ice was used to move tubes from one -20 to another...   :(   We need to test different glycerol concentrations next?<br>
 However, we had a nice surprise, already, in being able to revive some bioluminescent bacteria (which turned out to be ''Photobacterium phosphorum''!) after many months in an eppendorf tube in the frig!!  More [http://wiki.hackuarium.ch/w/Bioluminescence_investigations information about this is already] in the wiki.

@@ Line 44: / Line 44: @@
 * Always tidy things away after manipulations are complete, and if anything needs to stay out on a bench, or in incubators, remember to always '''leave signed and dated notes'''!
 * Do you have questions? <br>Maybe you can't reach Rachel or Javier, or want to find out more before even trying to ask them?? <br> There is a service in the DIYBio community for this [http://ask.diybio.org/ Ask a Biosafety Professional]!  Use it! (actually in the midst of a double check to see if it still works -nov23)<br>
-* '''Remember, being clueless is no excuse!!'''
+* '''Remember, being clueless is no excuse!!  there are many ways to find information.'''
 * The Standard Operating Procedures for appropriate use of the new space are being developed, and build upon what was [http://wiki.hackuarium.ch/w/P1_SOP previously decided during preparations to establish a P1 laboratory for Hackuarium members] already back in 2015/6. ([http://wiki.hackuarium.ch/w/Instru_Main Some old equipment] still has useful QR codes that lead to information in this wiki, too! Bravo, les poulpes!)
 Here are some [http://wiki.hackuarium.ch/w/New_SOPs new SOPs] for use of the small sterile hood and for use of the ultracentrifuge in the new space.

@@ Line 5: / Line 5: @@
 The latter can be used for some biology work, especially higher resolution microscopy (with Raspberry Pi camera on the old Zeiss scope) and bioluminescence studies, but no growth of cultures is allowed there.
 (there is a carpet!)
-The overall map of our coop space is shown on the right, and below that is an updated P1 lab map. [[File:Crochy20Ecublens - spaceallocsmaller15may19.png|400px|thumb|right| map of the space]]  [[File:P1lab map png.png|thumb|The P1 lab has controlled access for potential work on GMOs]]<br>
+The overall map of our coop space is shown on the right, and below that is an updated P1 lab map. [[File:Crochy20Ecublens - spaceallocsmaller15may19.png|400px|thumb|right| map of the space]]  [[File:P1lab map png.png|thumb|The P1 lab has controlled access for potential work on GMOs. Plan your experiments in advance!]]<br>
 The biolab is located in a south facing room of over 22m2,  with three windows and a linoleum floor. <br>
 ''It must be stressed that this is a provisional space, with the building already destined for destruction in the next 3-5 years (or so they say!). <br> Thus, we still need to find someplace more sustainable, and any help in this regard would be greatly appreciated!'' <br>

@@ Line 14: / Line 14: @@
 =Biosafety Officers=
-Rachel Aronoff and Olivier Emery continued their roles as biosafety officers in the new space, until Olivier got too busy and left the board in June 2020.  Maybe '''you''' would like to become our new deputy biosafety officier? :)  <br>
+Rachel Aronoff and Olivier Emery continued their roles as biosafety officers in the new space, until Olivier got too busy and left the board in June 2020, after which Javier Ortiz became the deputy.  Maybe '''you''' would like to become a second deputy biosafety officier? :)  <br>
 If you need to contact them in particular about any proposed activities or projects in the biolab, please use this email address: biolab@hackuarium.ch <br>
@@ Line 38: / Line 38: @@
 = Further information =
 * The lab layout is modular and flexible, with the tall bench useful in both orientations, depending on needs.
 * Much equipment can be kept on shelves, and taken out to use when needed.
 * All projects will have a box for their special equipment and stocks. Ask for a new one, if you need it!
 * Always tidy things away after manipulations are complete, and if anything needs to stay out on a bench, or in incubators, remember to always '''leave signed and dated notes'''!
-* Do you have questions? <br>Maybe you can't reach Rachel or Olivier, or want to find out more before even trying to ask them?? <br> There is a service in the DIYBio community for this [http://ask.diybio.org/ Ask a Biosafety Professional]!  Use it! (And double check!)<br>
+* Do you have questions? <br>Maybe you can't reach Rachel or Javier, or want to find out more before even trying to ask them?? <br> There is a service in the DIYBio community for this [http://ask.diybio.org/ Ask a Biosafety Professional]!  Use it! (And double check!)<br>
 * '''Remember, being clueless is no excuse!!'''
 * The Standard Operating Procedures for appropriate use of the new space are being developed, and build upon what was [http://wiki.hackuarium.ch/w/P1_SOP previously decided during preparations to establish a P1 laboratory for Hackuarium members] already back in 2015/6. ([http://wiki.hackuarium.ch/w/Instru_Main Some old equipment] still has useful QR codes that lead to information in this wiki, too! Bravo, les poulpes!)