BeeMoSeq - Revision history

Rachel at 13:59, 6 February 2023

2023-02-06T13:59:03Z

Javi at 11:22, 30 December 2021

2021-12-30T11:22:04Z

Rachel at 12:42, 20 October 2021

2021-10-20T12:42:34Z

Rachel at 12:37, 20 October 2021

2021-10-20T12:37:22Z

Javi at 14:50, 17 October 2021

2021-10-17T14:50:30Z

Javi at 14:49, 17 October 2021

2021-10-17T14:49:58Z

Javi: Created page with "The current floral characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each p..."

2021-10-17T14:49:02Z

Created page with "The current floral characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each p..."

New page

The current floral characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each pollen structure and the further counting to determine if a honey is monofloral or polyfloral. Amazingly each flower produces a specific pollen with a defined structure, but as the honey is made from many flowers its health properties will depend from the flowers proportions. However, this technique takes many hours, it is carried out by specialists and it lacks from robustness sometimes. Therefore in Hackuarium we have thought to use a molecular approach to know exactly the many floral species present in a honey sample.
The goal of BeeMoSeq is to link the pollen with the flower by using a molecular barcode. Luckily for us, to achieve this goal Hackuarium has a good background in molecular biology, carrying out in the past two workshops for the community [https://wiki.hackuarium.ch/w/Open-Food-DNA Open Food Repo].
BeemoSeq represents a project to complement [https://wiki.hackuarium.ch/w/BeeMoS BeeMoS] only that specializing in the molecular barcoding from one important part from the hive, the honey.

← Older revision		Revision as of 13:59, 6 February 2023
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	The goal of BeeMoSeq is to create a link between the pollen and the flower origin by using a molecular barcode. Luckily for us, to achieve this goal Hackuarium has a good background in molecular biology, carrying out in the past two workshops for the community as the two versions of [https://wiki.hackuarium.ch/w/Open-Food-DNA Open Food Repo].		The goal of BeeMoSeq is to create a link between the pollen and the flower origin by using a molecular barcode. Luckily for us, to achieve this goal Hackuarium has a good background in molecular biology, carrying out in the past two workshops for the community as the two versions of [https://wiki.hackuarium.ch/w/Open-Food-DNA Open Food Repo].

−	We have already done a first PCR to show we can amplify the Rubisco's gene used for much plant species classifications, but we need to show we can get pollen from honey for the same molecular amplification. Luckily, we have four honey bottles that ~~have~~ been classically characterized, so we can compare the molecular IDs to those of the pollen expert!	+	We have already done a first PCR to show we can amplify the Rubisco's gene used for much plant species classifications, but we need to show we can get pollen from honey for the same molecular amplification. Luckily, we have four honey bottles with pollen that has been classically characterized, so we can compare the molecular IDs to those of the pollen expert!

@@ Line 1: / Line 1: @@
 The current characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each pollen structure and the further counting to determine if a honey is monofloral or polyfloral. Amazingly each flower produces a specific pollen with a defined structure, but as the honey is made from many flowers its health properties will depend upon each flowers proportions. However, this technique takes many hours, it is carried out by specialists and it can lack robustness. Therefore in Hackuarium we have thought to use a molecular approach to know exactly which of the many pollen species are present in a honey sample.
-BeemoSeq represents a project to complement [https://wiki.hackuarium.ch/w/BeeMoS BeeMoS] - in this case specializing in the molecular barcoding from the important product from hives, the honey.
+BeemoSeq represents a project to complement [https://wiki.hackuarium.ch/w/BeeMoS BeeMoS] - in this case specializing in the molecular barcoding from one of the many product coming from a bee hive... the honey.
-The goal of BeeMoSeq is to create a link between the pollen and the flower origin by using a molecular barcode. Luckily for us, to achieve this goal Hackuarium has a good background in molecular biology, carrying out in the past two workshops for the community [https://wiki.hackuarium.ch/w/Open-Food-DNA Open Food Repo].
+The goal of BeeMoSeq is to create a link between the pollen and the flower origin by using a molecular barcode. Luckily for us, to achieve this goal Hackuarium has a good background in molecular biology, carrying out in the past two workshops for the community as the two versions of [https://wiki.hackuarium.ch/w/Open-Food-DNA Open Food Repo].
-We have already done a first pcr to show we can amplify the rubisco gene used for much plant species classifications, but we need to show we can get pollen from honey for the same molecular amplification.  Luckily, we have 4 honey bottles that have been classically characterized, so we can compare the molecular ids to those of the pollen expert!
+We have already done a first PCR to show we can amplify the Rubisco's gene used for much plant species classifications, but we need to show we can get pollen from honey for the same molecular amplification. Luckily, we have four honey bottles that have been classically characterized, so we can compare the molecular IDs to those of the pollen expert!

@@ Line 1: / Line 1: @@
-The current characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each pollen structure and the further counting to determine if a honey is monofloral or polyfloral. Amazingly each flower produces a specific pollen with a defined structure, but as the honey is made from many flowers its health properties will depend from the flowers proportions. However, this technique takes many hours, it is carried out by specialists and it lacks from robustness sometimes. Therefore in Hackuarium we have thought to use a molecular approach to know exactly the many floral species present in a honey sample.
+The current characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each pollen structure and the further counting to determine if a honey is monofloral or polyfloral. Amazingly each flower produces a specific pollen with a defined structure, but as the honey is made from many flowers its health properties will depend upon each flowers proportions. However, this technique takes many hours, it is carried out by specialists and it can lack robustness. Therefore in Hackuarium we have thought to use a molecular approach to know exactly which of the many pollen species are present in a honey sample.
 The goal of BeeMoSeq is to create a link between the pollen and the flower origin by using a molecular barcode. Luckily for us, to achieve this goal Hackuarium has a good background in molecular biology, carrying out in the past two workshops for the community [https://wiki.hackuarium.ch/w/Open-Food-DNA Open Food Repo].
-BeemoSeq represents a project to complement [https://wiki.hackuarium.ch/w/BeeMoS BeeMoS] only that specializing in the molecular barcoding from one important part from the hive, the honey.
+We have already done a first pcr to show we can amplify the rubisco gene used for much plant species classifications, but we need to show we can get pollen from honey for the same molecular amplification.  Luckily, we have 4 honey bottles that have been classically characterized, so we can compare the molecular ids to those of the pollen expert!

@@ Line 1: / Line 1: @@
-The current floral characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each pollen structure and the further counting to determine if a honey is monofloral or polyfloral. Amazingly each flower produces a specific pollen with a defined structure, but as the honey is made from many flowers its health properties will depend from the flowers proportions. However, this technique takes many hours, it is carried out by specialists and it lacks from robustness sometimes. Therefore in Hackuarium we have thought to use a molecular approach to know exactly the many floral species present in a honey sample.
+The current characterization of pollen contained in honey is carried out by melissopalynology, which is a meticulous technique based in the identification of the each pollen structure and the further counting to determine if a honey is monofloral or polyfloral. Amazingly each flower produces a specific pollen with a defined structure, but as the honey is made from many flowers its health properties will depend from the flowers proportions. However, this technique takes many hours, it is carried out by specialists and it lacks from robustness sometimes. Therefore in Hackuarium we have thought to use a molecular approach to know exactly the many floral species present in a honey sample.
 The goal of BeeMoSeq is to create a link between the pollen and the flower origin by using a molecular barcode. Luckily for us, to achieve this goal Hackuarium has a good background in molecular biology, carrying out in the past two workshops for the community [https://wiki.hackuarium.ch/w/Open-Food-DNA Open Food Repo].
 BeemoSeq represents a project to complement [https://wiki.hackuarium.ch/w/BeeMoS BeeMoS] only that specializing in the molecular barcoding from one important part from the hive, the honey.