https://wiki.hackuarium.ch/api.php?action=feedcontributions&user=Randogp&feedformat=atomHackuarium - User contributions [en]2024-03-29T01:59:09ZUser contributionsMediaWiki 1.35.3https://wiki.hackuarium.ch/index.php?title=20180601_GMO_Detective_Workshop_01&diff=1106420180601 GMO Detective Workshop 012018-06-02T09:53:22Z<p>Randogp: </p>
<hr />
<div>Everything we eat, everyone we love, sickness and health, the entire living world is written in DNA. Few today understand, but many fear, this new power that is quickly maturing.<br />
<br />
Organised by [[User:Randogp | Gianpaolo Rando]]<br><br />
<br />
* Main language this week: '''English'''<br><br />
* On parle français, parliamo italiano, falamos portugues<br />
* Theme: '''GMO Detective Workshop'''<br />
* Date: '''Friday 01.06.2018, afternoon''', presentation and discussion start at 19:30<br />
* Location: Room "P1", Hackuarium, 2nd Floor<br />
<br />
Guy Aidelberg from CRI Paris will join our P1 lab and show the participants how to DNA test if there are GMOs (Genetically Modified Organisms) in their food.<br />
<br />
Bring your food for the test, we bring the pipets and the safety rules.<br />
<br />
While pipetting, we will discuss together about food, DNA, foodtech, GMOs, and participatory science.<br />
<br />
GMO Detective is a low cost and open Citizen Science project for detection of transgenic elements in food and plants.<br />
https://gmodetective.com/<br />
<br />
<br />
RSVP: https://www.meetup.com/hackuarium/events/250778421/<br />
<br />
[[File:GMOdetective1.png|500px|thumb|left|Discussing first principles with a beer on the terrasse]]<br />
[[File:GMOdetective2.png|500px|thumb|left|Doing the real forensic analysis with DNA]]<br />
<br />
[[Category:Events]]<br />
[[Category:Workshop]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20180601_GMO_Detective_Workshop_01&diff=1106320180601 GMO Detective Workshop 012018-06-01T18:53:01Z<p>Randogp: </p>
<hr />
<div>Everything we eat, everyone we love, sickness and health, the entire living world is written in DNA. Few today understand, but many fear, this new power that is quickly maturing.<br />
<br />
Organised by [[User:Randogp | Gianpaolo Rando]]<br><br />
<br />
* Main language this week: '''English'''<br><br />
* On parle français, parliamo italiano, falamos portugues<br />
* Theme: '''GMO Detective Workshop'''<br />
* Date: '''Friday 01.06.2018, afternoon''', presentation and discussion start at 19:30<br />
* Location: Room "P1", Hackuarium, 2nd Floor<br />
<br />
Naiane from CRI Paris will join our P1 lab and show the participants how to DNA test if there are GMOs (Genetically Modified Organisms) in their food.<br />
<br />
Bring your food for the test, we bring the pipets and the safety rules.<br />
<br />
While pipetting, we will discuss together about food, DNA, foodtech, GMOs, and participatory science.<br />
<br />
GMO Detective is a low cost and open Citizen Science project for detection of transgenic elements in food and plants.<br />
https://gmodetective.com/<br />
<br />
<br />
RSVP: https://www.meetup.com/hackuarium/events/250778421/<br />
<br />
[[File:GMOdetective1.png|500px|thumb|left|Discussing first principles with a beer on the terrasse]]<br />
[[File:GMOdetective2.png|500px|thumb|left|Doing the real forensic analysis with DNA]]<br />
<br />
[[Category:Events]]<br />
[[Category:Workshop]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=File:GMOdetective2.png&diff=11062File:GMOdetective2.png2018-06-01T18:49:42Z<p>Randogp: </p>
<hr />
<div></div>Randogphttps://wiki.hackuarium.ch/index.php?title=File:GMOdetective1.png&diff=11061File:GMOdetective1.png2018-06-01T18:49:09Z<p>Randogp: </p>
<hr />
<div></div>Randogphttps://wiki.hackuarium.ch/index.php?title=Main_Page&diff=11060Main Page2018-06-01T18:47:17Z<p>Randogp: /* Upcoming Hackuarium Events */</p>
<hr />
<div>Welcome to the wiki of the [http://www.hackuarium.ch/ Hackuarium] association! <br><br />
<br />
<br />
At Hackuarium, we want to bring biology (and biologists) to the world, and the real world back to biology. Our laboratory is an excuse to meet and discuss, build and develop ideas in a neutral, open, noncompetitive and not-for-profit environment.<br><br />
<br />
This page was launched during our [[1st Ever Hackuarium Wiki Sprint]] that took place on 7 December 2014. As you might notice quickly, almost everything here is a work-in-progress.<br />
<br />
Scroll down and find out what this is all about!<br />
<br />
<div><br />
<big>''[[Hackuarium | Cliquez ici pour accéder à la version française de ce wiki.]]''</big> <br />
</div><br />
<br />
[[File:PosterHackuarium.png |535px|thumb|right|Hackuarium Poster (Not-for-printing version!!! see Section 7.9 [[Main_Page#Visual_Identity | Visual Identity]] for full size printing version)]] <br />
<br />
__TOC__<br />
<br />
<br><br />
<br />
=What is DIYbio?=<br />
DIYbio (Do-It-Yourself biology) is a movement that wants to free the practice of biological research and innovation from the institutional and industrial laboratories. Pursued both by amateurs and professional biologists, DIYbio is developing low-cost and low-tech solutions to problems identified by the community.<br />
<br />
The movement is characterised by an extremely diverse set of practices and participants. Some call themselves 'biohackers', in reference to the hacker culture. The quick solution (even if unconventional) is what is meant by hacks...<br />
<br />
[http://DIYbio.org DIYbio.org] and [http://DIYbio.eu DIYbio.eu] are the portals of the international and european communities, respectively.<br />
<br />
For more information, visit the [http://www.wikipedia.org Wikipedia] page dedicated to [https://en.wikipedia.org/wiki/DIYbio DIYbio].<br><br />
<br />
We actually believe more and more in doing DIT (Do-It-Together) Science, not just 'DIYbio,' as it reflects better the scope of the trans-disciplinary projects we do (often not only biology).<br><br />
<br />
<br><br />
<br />
=What is Hackuarium?=<br />
<br />
Hackuarium is a not-for-profit association aiming at democratizing science through public engagement.<br><br />
<br />
Our laboratory in Renens (Switzerland) is open to anyone sharing the values of the association and who is dedicated to follow the [http://diybio.org/codes/draft-diybio-code-of-ethics-from-european-congress/ DIYbio Europe ethical guidelines].<br><br />
<br />
Our projects are initiated and carried out by scientists and non-scientists from a variety of backgrounds. They are passionate about tinkering with biology in particular, and technology in general. Some are engineers, architects, designers, IT and computer scientists or retired professionals, but others have no scientific education. They are mostly citizens interested in open and participatory research and innovation, outside the constraints of traditional institutions.<br><br />
<br />
Hackuarium members want to investigate new ways of carrying out interdisciplinary research and innovation, by making their results accessible (low-cost), simple and easily reproducible (low-tech) and by promoting an open source philosophy.<br><br />
<br />
<br><br />
<br />
=Where are we?=<br />
<br />
Hackuarium is proudly using infrastructures provided by [http://www.univercite.ch UniverCité], an unconventional innovation hub that opened in May 2014 in the IRL building in Renens. Our equipment is mostly upcycled material from institutions and industries from western Switzerland. We have documented our working environment as it has evolved.<br><br />
<br />
<br />
Between December 2015 and January 2017, UniverCité regrouped our laboratory, a workshop and coworking space.<br><br />
<br />
The original space was under construction work from January until June 2017, and we temporarily relocated on the first floor of the same building.<br><br />
<br />
Since July 2017, we are back on the second floor of what is now called the "Ateliers de Renens" Chemin du Closed 5, 1020 Renens. We have a biohackerspace where (almost) everything goes, and a P1 facility for synthetic bio enthusiasts and more. <br> Check it out during one of our open nights, every Wednesday... <br><br />
<br />
Renens is a hopping hub between Lausanne and Geneva. You can get to us by train from the airport in Geneva within an hour.<br><br />
<br />
<br><br />
<br />
=Who are we?=<br />
<br />
Our members reflect the diversity that is in and around Lausanne/Renens.<br><br />
<br />
[[People | Check us out]], and find out what makes Hackuarium a good mix.<br><br />
<br />
==Committee==<br />
<br />
The current [[Board | committee]] is composed of the following people:<br><br />
<br />
* President: [[User: Rachel | Rachel Aronoff]] (also Citizen Science coordinator and Biosafety Officer)<br />
* Lab Manager: [[User: Gustavo Santamaria| Gustavo Santamaria]]<br />
* Secretary and Treasurer: [[User: luchenry | Luc Henry]]<br />
* Shadow Treasurer: Masoud Alipour<br />
* Deputy Biosafety Officer and Communications Coordinator, and Shadow Secretary: Olivier Emery<br />
* Art and Science coordinator: [[User: Vlorenzo | Vanessa Lorenzo]] <br />
* System Administrator: [[User:Sam | Sam Sulaimanov]]<br />
* Talent scout and evangelist for Academy: [[User:Alpittet | Anne-Laure Pittet]]<br />
* Talent scout and evangelist for Entrepreneurs: [[User:Randogp | Gianpaolo Rando]]<br />
<br />
<br />
The Agenda of the last board meeting can be found [[20180523_Board_Meeting | here]].<br><br />
The Agenda of the next board meeting can be found [[20180627_Board_Meeting | here]].<br><br />
<br />
==Members and Membership==<br />
<br />
'''Do you have a project in mind? Do you just want to support us?'''<br><br />
<br />
Hackuarium operates on a membership basis, even though all events are open to anyone, including non-members.<br><br />
<br />
The monthly membership fee is 20 CHF. It gives 24/7 access to the lab.<br><br />
<br />
We would love to have you as a member! Just follow the instructions below.<br><br />
<br />
===Want to join?===<br />
<br />
* We have a [https://docs.google.com/forms/d/e/1FAIpQLSdoyS8HmsuZACYp-q79pLzxR9BPA60HbyL6ZR8x7h7x3eqbhQ/viewform new form on the website], to fast-track joining the community, if you are keen, perhaps after a few #OpenHackuarium experiences (every Wednesday evening is open to the public).<br />
<br />
* Read some helpful info [[I_m_new | here]].<br />
<br />
* Write an email to hello@hackuarium.ch and give us a short description of:<br />
** Yourself, your interests, your background<br />
** What you would like to learn or do at Hackuarium<br />
<br />
==Friends of Hackuarium==<br />
<br />
Spaces:<br />
::<li>[[UniverCité]]<br />
::<li>[https://onlfait.ch/fablab/ FabLab Onl'fait, Genève]<br />
::<li>[http://lapaillasse.org La Paillasse, Paris]<br />
::<li>[https://lapaillassaone.wordpress.com Paillasse Saône, Lyon]<br />
::<li>[http://foundry.bio The BioFoundry, Sydney]<br />
::<li>[http://www.bioquisitive.org.au Bioquisitive, Melbourne]<br />
<br />
Projects:<br />
::<li>[http://octanis.org Octanis]<br />
::<li>[http://hybridoa.org Hybridoa]<br />
::<li>[http://apéro-sciences.ch/ Apéro Sciences]<br />
::<li>[http://www.genomicintegrity.org AGiR!]<br />
::<li>[[Exodes_Urbains | Exodes Urbains]]<br />
::<li>[http://echopen.org/index.php?title=Main_Page EchOpen]<br />
<br />
<br><br />
<br />
=Events=<br />
<br />
Our lab and space are open to the public. Please come and have a chat! And if you have any questions [mailto:hello@hackuarium.ch get in touch!]<br />
<br />
<big>'''[[Open_Hackuarium | OpenHackuarium]]: Every Wednesday, 19:00-22:00'''</big><br />
<br />
You want to organise an event at Hackuarium, or with the Hackuarium community? We have guidelines below.<br><br />
<br />
==Upcoming Hackuarium Events==<br />
<br><br />
<br />
'''2018'''<br><br />
:<li>'''2018.07.25''' Wednesday 18:30-19:30: [[20180725_Board_Meeting | Board Meeting]]<br />
:<li>'''2018.06.27''' Wednesday 18:30-19:30: [[20180627_Board_Meeting | Board Meeting]]<br />
:<li>'''2018.06.01''' Friday afternoon: [[20180601_GMO_Detective_Workshop_01 | GMO Detective Workshop]] - with Guy Aidelberg from CRI Paris. Bring your food and discover if it contains GMOs.<br />
:<li>'''2018.05.31''' Thursday 18:30-12:30: Hackuarium will be paying a visit to FabLab Onl'fait and ImpactHub Geneva<br />
<br />
<br><br />
<br />
or follow our [https://calendar.google.com/calendar/embed?src=mgvq9goephpmna0kb7rj9ipei8%40group.calendar.google.com&ctz=Europe/Zurich google calendar]<br><br />
<br />
==Other Upcoming Events at [http://www.univercite.ch UniverCité]==<br />
<br><br />
<br />
==Other Upcoming Events==<br />
<br />
==Past Hackuarium Events==<br />
<br><br />
You can find the complete list of our '''past events''' [[past events | here]].<br />
<br />
==Event Organisation== <br />
<br />
You want to organise an event? Go for it!<br><br />
<br />
Help us spread the love for tech, science, art and design.<br><br />
<br />
Here is a "Wiki express" so that everyone has the basics: <br><br />
<br />
*Send an email to board@hackuarium.ch — to make sure there is no conflict with another event<br />
*Someone on the board will check that the space is free<br />
*Build a wiki page for the event (see template below)<br />
*Our 'communications coordinator' will get in touch to ask for content and will make sure the announcements are made on all the social media outlets for Hackuarium!<br />
*In case you need a microgrant to cover the costs (up to 300.-), send an email to board@hackuarium.ch with a description (what, when, who) and a preliminary budget.<br />
*When all this is arrange, talk to the community, to spread the word —> us the slack '''#_uc-events''' channel<br />
*Is it a WORKSHOP? All the previous & make sure you get your logistics right! (and use the tag at the bottom of the page)<br />
*Is there a SIMILAR workshop ALREADY planned? If yes, negotiate dates and: Do you target the SAME people? If yes, negotiate. If not, no problem. It maybe interesting to see what others do.<br />
<br />
===Template for an Hackuarium event===<br />
<br />
First, create a line for your event on the main page under the [Main_Page#Events | Event section] using the following line:<br />
<br />
<pre><br />
:<li>'''201X.XX.XX''' Wednesday 19:00-22:00: [[201XXXXX_TITLE | TITLE]] - SHORT DESCRIPTION<br />
</pre><br />
<br />
Make sure the date, time and title are provided. Save the main page.<br><br />
The event will appear with a link. By clicking on this link you will create the event page.<br><br />
The following must be added to the event page (replace XXX by appropriate values):<br><br />
<br />
'''Header'''<br><br />
<br />
<pre><br />
Open Hackuarium #XX - TITLE<br><br />
Organisé par XXX<br><br />
<br />
'''ENGLISH BELOW''' <br><br />
<br />
* Langue principale cette semaine: '''Anglais'''<br><br />
* Thème : '''TITLE'''<br />
* Date : '''Mercredi XX.XX.2018, 19h''', présentation et discussion à 19:30 et jusqu'à 21h<br />
* Lieu : Salle "XXX", UniverCité, 3ème étage<br />
<br />
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -<br><br />
<br />
* Main language this week: '''English'''<br><br />
* Theme: '''TITLE'''<br />
* Date: '''Wednesday XX.XX.2018, 19:00''', presentation and discussion start at 19:30<br />
* Location: Room "XXX", UniverCité, 3rd Floor<br />
</pre><br />
<br />
'''Main Text'''<br><br />
<br />
Add a text description providing more details about the event.<br><br />
<br />
You include pictures in the same way as in any other page using the following code:<br />
<br />
<pre><br />
[[File:XXX.png|500px]]<br />
</pre><br />
<br />
Add the following tags at the bottom of the page:<br />
<br />
<pre><br />
[[Category:Events]]<br />
[[Category:OpenHackuarium]]<br />
</pre><br />
<br />
And eventually:<br />
<br />
<pre><br />
[[Category:Workshop]]<br />
</pre><br />
<br />
<br><br />
<br />
=Projects=<br />
<br />
Here, you will find the projects going on at Hackuarium.<br />
<br />
== Active Projects ==<br />
<br />
* [[BeerDeCoded]]<br />
* [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Living Instruments]]<br />
* [[Micro to Macro Water Pollution]] - with [http://www.hammerdirt.ch/ Hammerdirt] and [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Spectro-pointer]]<br />
* [[Darty_Monkeys]]<br />
* [[Grow%26Mix_Bioink]]<br />
* [[AGiR! for genomic integrity]]<br />
* [[Moss Menageries with AGiR!]]<br />
* [[Magnesium tests with AGiR!]]<br />
* [[Urban gardens with rhizobial bacteria]]<br />
* [[Open_Source_Bioreactor | Open Source Bioreactor]]<br />
* [[Bioreactor | Steady state bioreactor]]<br />
* [[Workshop_Bioprinter| Workshop Bioprinter]]<br />
* [[Terraforming Alkalin Experiments | Terraforming Alkaline Experiments]]<br />
* [http://wiki.hackuarium.ch/w/Diy-transilluminator DIY Transilluminator]<br />
* [[Stress Analyzer]]<br />
<br />
== Archived Projects ==<br />
<br />
* [[Octanis]] - with more information about their Rover / Balloon [http://octanis.org/rover here]<br />
* [http://wiki.hackuarium.ch/w/Crowdfunding2017 Crowdfunding]<br />
* [[HTGAA | HTGAA]]<br />
* [[Hacktion | Hacktion challenges]]<br />
* [[Open-Food-DNA |Open-Food-DNA]]<br />
* [[Bience | Bience (Beer and Science!)]]<br />
* [[Mycelium Structures]]<br />
* [[Live Type]]<br />
* [[Projects:LivingLab Design|LivingLab Design]]<br />
* [[Projects:bioluminescence|Bioluminescence]]<br />
* [[Projects:Quantitative Anthropology|Quantitative Anthropology]]<br />
* [[Projects:Impression 3D|Impression 3D]]<br />
* [[Projects:Dépollution des stands de tirs Suisses]]<br />
* [[Incubateur bon marché]]<br />
* [[Space Seed]]<br />
* [[Reception d'images satellites]]<br />
* [[Edible wall]]<br />
* [[ZéBU CH]]<br />
* [[Compost Bacteria]]<br />
* [[Protein purification systems]]<br />
* [[unmapping]]<br />
* [[Colab Biomaterials | Colab Biomaterials]]<br />
<br />
<br><br />
<br />
= Practical Information =<br />
<br />
== Rules at Hackuarium ==<br />
<br />
We have very few rules at Hackuarium. The goal of the association is to provide freedom to its members, so they can explore beyond what is allowed elsewhere. However, we want members and anyone participating in our activities to feel comfortable and respected. <br><br />
<br />
[[Life_Main | This page]] describes the code of conduct for Hackuarium members.<br><br />
<br />
== Use of P1 laboratory ==<br />
As of November 2017, Hackuarium is officially hosting its own P1 laboratory. While we still lack some essential infrastructure (-80 freezer, ventilated fume hood), we are working on it... <br />
More can be found out about use of this facility by following the next link...<br />
[[P1_Lab_Activities| P1 Lab Activities]]<br />
<br />
== [[Protocols | Protocols]] ==<br />
All the protocols that we use at Hackuarium will be found [[ BioP_Main | here ]] (work in progress).<br />
They are detailed and structured so that the main focus is on applications. The idea is to give crucial information on how to apply the most common techniques of the Biology toolbox. The protocols are structured as follow:<br />
::<li> A scheme describing each <br />
::<li> A brief description of the protocol and its use<br />
::<li> A description of the different ways of carrying out the protocol. Ideally there should be a description of a commercial kit and a DIY kit.<br />
::<li> A description of the safety issues and best practice<br />
::<li> Information specific to Hackuarium and where to find the components, tools and reagents.<br />
<br />
== Instruments available ==<br />
[[Instru_Main | This page ]] describes all the instruments available at Hackuarium (work in progress..), their location and how to use them.<br />
<br />
=== Up-to-date list of instruments we still need ===<br />
This [https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing on-line document] contains all the instruments, tools and consumables we are looking for. We have a standard donation contract ([[Contrat_de_donation|contrat de donation]], in French) and if your organisation happen to discard a piece of equipment we are (or may be) looking for, [mailto:hello@hackuarium.ch contact us]!<br />
<br />
== Where we shop ==<br />
<br />
As a general rule, we do not buy anything. We try to recycle, upcycle, bicycle, epicycle anything we can, or ask around if anyone can provide supplies for free.<br><br />
<br />
In some cases it does not work out, and so these are the place where we can find what we need.<br><br />
<br />
In any case, '''Please contact [[User:Luchenry|Luc Henry]] if you need to order anything in the name of Hackuarium.'''<br><br />
<br />
===Labware and Reagents===<br />
<br />
Consumables and some chemicals can be purchased from [http://www.huberlab.ch Huber Lab].<br><br />
More chemicals can be obtained from [https://www.carlroth.com/ch/en Carl Roth].<br><br />
If necessary, other chemicals and biochemicals can be obtained from [https://www.sigmaaldrich.com Sigma Aldrich].<br><br />
Molecular biology tools can be purchased from [https://www.qiagen.com/ch/ Qiagen].<br><br />
<br />
Swiss-based [http://www.smiples.ch Smiples] has a very extensive listing of second hand laboratory and technical items. These can be bought from their online shop or from [https://www.ricardo.ch/online-shop/smiples/?SellerNickName=Smiples Ricardo].<br><br />
But most laboratory hardware can be obtained from our network of friends and partners. Get in touch with [[User:Luchenry|Luc Henry]] if you need anything specific and we'll do our best to find it.<br><br />
<br />
===Organic===<br />
Algae, protozoa, acuatic life and educational kits: [https://www.sciento.co.uk/ | Sciento]<br><br />
Bacteria, global biosource center (Hackuarium has a customer account, shop through our Biosecurity manager): [https://www.lgcstandards-atcc.org/?geo_country=ch | ATCC]<br />
<br />
===Hardware===<br />
Options for electronic parts:<br />
<br />
* [http://www.ebay.com Ebay] (international)<br />
* [http://www.aliexpress.com AliExpress] (China)<br />
* [https://www.adafruit.com Adafruit] (UK)<br />
* [http://www.seeedstudio.com/depot/ Seeedstudio] (China)<br />
* [http://www.tmart.com/ Tmart] (Hong Kong)<br />
* [https://www.pi-shop.ch/ Rasberry pi shop] (Switzerland)<br />
* [http://www.distrelec.ch/ Distrelec] (Switzerland)<br />
<br />
==How to built a new project page on our Wiki==<br />
<br />
If you want to start or edit a project page on Hackuarium's wiki, please follow these [[Editing the wiki | instructions]].<br><br />
<br />
== ReadingList ==<br />
<br />
=== The DIY biology / Biohacking movement ===<br />
<br />
<li>[http://delfanti.org/biohackers/ Biohackers. The politics of open science] by Alessandro Delfanti (2013) London: Pluto Press.<br />
::[http://delfanti.org/wp-content/uploads/2013/05/Science-2014-Golinelli-521.pdf A review] by [[User:luchenry | Luc Henry]]<br />
<li>[http://link.springer.com/article/10.1186/s40504-016-0039-1 DIY-Bio – economic, epistemological and ethical implications and ambivalences] by Jozef Keulartz and Henk van den Belt (2016) Life Sciences, Society and Policy<br />
<li>[http://www.biobasedworldnews.com/biohacking-everything-you-need-to-know-about-diy-biology Biohacking] everything you need to know about diy biology, (Accessed 05.2016)<br><br />
<li>[http://www.prnewswire.com/news-releases/genspace-nyc-receives-350000-in-support-from-the-simons-foundation-300237457.html Genspace gets funding], PRNewswire, (Accessed 05.2016)<br><br />
<li>[http://www.nature.com/news/governance-learn-from-diy-biologists-1.19507 Learn from DIY biologists], Todd Kuiken, Nature, (Accessed 05.2016)<br />
<br />
<br><br />
<br><br />
<br />
And a much more complete list by [https://www.kuleuven.be/wieiswie/en/person/00105850 Massimiliano Simons], KU Leuven:<br />
<br />
<br><br />
<br><br />
<br />
<li>[http://www.the-scientist.com/?articles.view/articleNo/34457/title/The-Rebirth-of-DIYbio/ The Rebirth of DIYbio] by Jef Akst, The Scientist, March 2013<br />
<li>[https://www.newscientist.com/article/mg21829236-300-citizen-scientist-out-of-the-lab-and-onto-the-streets/ Out of the lab and onto the streets] by Kat Austen, New Scientist, June 2013<br />
<li>[https://academic.oup.com/bioscience/article/65/1/112/379448/DIYbio-Alternative-Career-Path-for-Biologists DIYbio - Alternative Career Path for Biologists?] by Beth Baker, BioScience (2015) 65 (1): 112<br />
<li>[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1109.html From synthetic biology to biohacking: are we prepared?] by Gaymon Bennett, Nils Gilman, Anthony Stavrianakis & Paul Rabinow, Nature Biotechnology 27, 1109 - 1111 (2009)<br />
<li>[http://repository.jmls.edu/ripl/vol10/iss3/1/Patent Office as Biosecurity Gatekeeper: Fosering Responsible Science and Building Public Trust in DIY Science] by Brian J. Gorman, J. Marshall Rev. Intell. Prop. L. 423 (2011)<br />
<li>[http://wi.mobilities.ca/roberta-buiani-biolab-on-wheels-finding-a-space-for-a-diy-bio-lab-in-toronto/ Biolab-on-Wheels: finding a space for a DIY bio lab in Toronto] by Roberta Buiani, Journal of Mobile Media, February 2015<br />
<li>Charisius, Hanno & Friebe, Richard & Karberg, Sascha - Biohacking: Gentechnik aus der Garage<br />
<li>Curry, Helen Anne - From garden biotech to garage biotech: amateur experimental biology in historical perspective<br />
<li>Davies, Sarah R. & Karin Tybjerg & Louise Whiteley & Thomas Söderqvist - Co-curation as hacking: biohackers in Copenhagen's Medical Museion<br />
<li>Delfanti, Alessandro - Biohackers: The Politics of Open Science<br />
<li>Delfanti, Alessandro - Tweaking genes in your garage: biohacking between activism and entrepreneurship<br />
<li>Delfanti, Alessandro - Is Do-it-Yourself Biology Being Co-opted by Institutions?<br />
<li>Delfanti, Alessandro - Distributed biotechnology<br />
<li>Delfanti, Alessandro - Hacking genomes. The ethics of open and rebel biology<br />
<li>Delgado, Ana - DIYbio: Making things and making futures<br />
<li>Editorial - Empowering citizen scientists<br />
<li>Eggleson, Kathleen - Transatlantic Divergences in Citizen Science Ethics - Comparative Analysis of the DIYbio Code of Ethics Drafts of 2011<br />
<li>Gewin, Virginia - Independent streak<br />
<li>Golinelli, Stefano & Guido Ruivenkamp - Do-it-yourself biology: Action research within the life sciences<br />
<li>Grushkin, D., Kuiken, T., Millet, P - Seven Myths & Realities about Do-It-Yourself Biology<br />
<li>Grushkin, Daniel - Am I a biohazard?<br />
<li>Holloway, Dustin - Regulating Amateurs<br />
<li>Jefferson, Chaterine - Governing Amateur Biology: Extending Respnonsible Research and Innovation in Synthetic Biology to New Actors<br />
<li>Kean, Sam - A Lab of Their Own<br />
<li>Kelty, Christopher - Outlaw, hackers, victorian amateurs: diagnosing publich participation in the life sciences today<br />
<li>Kera, Denisa - Hackerspaces and DIYbio in Asia: connecting science and community with open data, kits and protocols<br />
<li>Kera, Denisa - Innovation regimes based on collaborative and global tinkering: Synthetic biology and nanotechnology in the hackerspaces<br />
<li>Kuiken, Todd - DIYbio: Low Risk, High Potential<br />
<li>Kuiken, Todd - Learn from Do-It-Yourself Biologists<br />
<li>Kuznetsov, Stacey - Expanding Our Visions of Citizen Science<br />
<li>Kuznetsov, Stacey & Alex Taylor & Tim Regan & Nicolas Villar & Eric Paulos - At the seams: DIYbio and opportunities for HCI<br />
<li>Kuznetsov, Stacey & Carrie Doonan & Nathan Wilson & Swarna Mohan & Scott E. Hudson & Eric Paulson - DIYbio Things: Open Source Biology Tools as Platforms fo rHybrid Knowledge Production and Scientific Participation<br />
<li>Landrain, Thomas & Meyer, Morgan & Perez, Ariel Martin & Sussan, Remi - Do-it-yourself biology: challenges and promises for an open science and technology movement<br />
<li>Lisa Z. Scheifele & Thomas Burkett - The First Three Years of a Community Lab: Lessons Learned and Ways Forward<br />
<li>McKenna, Phil - Rise of the garage genome hackers<br />
<li>Meyer, Morgan - Build your own lab<br />
<li>Meyer, Morgan - Domesticating and democratizing science: a geography of do-it-yourself biology<br />
<li>Meyer, Morgan - Hacking Life? The Politics and Poetics of DIY Biology<br />
<li>Meyer, Morgan - Bricoler, domestiquer et contourner la science<br />
<li>Nascimento, Susana & Angela Guimaraes Pereira & Alessia Ghezzi - From Citizen Science to Do It Yourself Science<br />
<li>NSABB - Strategies to Educate Amateur Biologists and Scientists in Non-life Science Disciplines About Dual use Research in the Life Science<br />
<li>Schmidt, Markus - Diffusion of synthetic biology<br />
<li>Scudellari, Megan - Biology Hacklabs<br />
<li>Seyfried, Günter & Pei, Lei & Schmidt, Markus - European do-it-yourself (DIY) biology: Beyond the hope, hype and horror<br />
<li>Sholette, Gregory - Disciplining the avant-garde: The United States versus the Critical Art Ensemble<br />
<li>Sipra Bihani & Michael Hartman & Florian Sobiegalla & Amanda rosenberg - Comparing network strutures of commercial and non-commercial biohacking online-communities<br />
<li>Söderberg, Johan & Delfanti, Alessandro - Hacking Hacked! The Life Cycles of Digital Innovation<br />
<li>Söderberg, Johan & Delfanti, Alessandro - Repurposing the hacker. Three temporalities of recuperation<br />
<li>Tocchetti, Sara - DIYbiologists as 'Makers' of Personal Biologies<br />
<li>Tocchetti, Sara - What kind of work we are doing now and what kind of work we want to do<br />
<li>Tocchetti, Sara & Sara Angeli Aguiton - Is an FBI Agent an DIY Biologist Like Any Oter? A Cultural Analysis of a Biosecurity Risk<br />
<li>Trojok, Rüdiger - Biohacking: Gentechnologie für Alle<br />
<li>van Boheemen, Pieter & Huib de Vriend - Do-it-yourself biology: Een verkenning van ontwikkelingen in Nederland<br />
<li>Wohlsen, Marcus - Biopunk: Solving Biotech's Biggest Problems in Kitchens and Garages<br />
<br />
=== Open Hardware ===<br />
<li>[http://www.nature.com/news/open-hardware-pioneers-push-for-low-cost-lab-kit-1.19518 Open-hardware, pioneers push for low-cost lab kit] by Elizabeth Gibney, Nature, (Accessed 05.2016)<br><br />
<li>[http://www.appropedia.org/Open-source_Lab#Examples Appropedia] a source of DIY instruments builds, Dr. Joshua Pearce, (Accessed 05.2016)<br><br />
<li>[http://www.ohwr.org/projects/fr_pcb_mm The PCB milling machine], Open Hardware Repository, (Accessed 05.2016)<br><br />
:: [https://pt.wikiversity.org/wiki/Pesquisa:Ferramentas_livres:Work_group_for_development_of_the_hyperobject_workbench More]<br />
<li>http://www.creativeapplications.net/processing/sensortape-3d-aware-dense-sensor-network-on-a-tape/, Sensory network on a roll of tape, Filip Visnjic, (Accessed 05.2016)<br><br />
<li>[http://oceanoptics.com/product/redeye-oxygen-sensing-patches/ RedEye Oxygen Sensing Patches], Ocean Optics, (Accessed 05.2016) <br><br />
<br><br />
<br />
=== Art ===<br />
<li> [http://www.booooooom.com/2016/04/04/mattia-menchetti-gives-wasps-coloured-paper-to-create-rainbow-nests/#more-78906 Rainbow wasp nests], Mattia Menchetti, (Accessed 05.2016)<br><br />
<li> [http://thecreatorsproject.vice.com/blog/dirty-beats-hear-music-generated-from-bacteria Dirty beats] making music with bacteria, Interspecifics(Accessed 05.2016)<br><br />
::[http://www.interspecifics.cc/-/ More1], [http://www.ncbi.nlm.nih.gov/pubmed/21239171, More2], [http://www.ncbi.nlm.nih.gov/pubmed/12501293, More3]<br><br />
<br><br />
<br />
=== Ideas/Concepts/Science ===<br />
<li>[http://ideas.ted.com/a-newly-drawn-tree-of-life-reminds-us-to-question-what-we-know/ The new tree of life] TED (Accessed 05.2016)<br><br />
<li>[https://medium.com/now-of-work/the-workplace-of-the-future-work-of-today-16c58df1fa29#.zhbu0c57f Rethinking work], Yann Heurtaux, (Accessed 05.2016)<br><br />
<li>[https://www.kickstarter.com/projects/910418035/plastic-bottle-cutter That plastic bottle cutter], Pavel & Ian, (Accessed 05.2016)<br><br />
<br><br />
<br />
=== Open Source ===<br />
<li>[https://blogs.openaire.eu/?p=882 Results held hostage], Francesco Mondada, (Accessed 05.2016)<br><br />
<br><br />
<br />
==Visual Identity==<br />
* [[Logo]]<br />
* [http://wiki.hackuarium.ch/images/9/9d/HackuariumPoster.pdf Our poster in pdf ready to print]<br />
* Working on a footage for videos (comming soon)<br />
<br />
== Media and Press ==<br />
Please check our [[Press kit]] out. It includes a prototype Press review of most of our media appearances over the years.<br />
<br />
== EU Paperwork ==<br />
We now have an identification number aka [https://ec.europa.eu/research/participants/portal/desktop/en/support/faqs/faq-1055.html Participant Identification Code (PIC)] from the European Participant Portal for any Horizon 2020 application. The Hackuarium PIC number is 907732306. It can be verified on [http://ec.europa.eu/research/participants/portal/desktop/en/organisations/register.html# the official search engine].<br />
<br />
== Wanted ==<br />
Do you want to contribute to making Hackuarium a diverse and lively community? Check out our wanted lists:<br />
::<li>Skills<br />
::<li>Ideas<br />
::<li>[https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing Equipment]<br />
::<li>Consumables<br />
<br />
<br><br></div>Randogphttps://wiki.hackuarium.ch/index.php?title=20180601_GMO_Detective_Workshop_01&diff=1094420180601 GMO Detective Workshop 012018-05-14T17:02:34Z<p>Randogp: </p>
<hr />
<div>Everything we eat, everyone we love, sickness and health, the entire living world is written in DNA. Few today understand, but many fear, this new power that is quickly maturing.<br />
<br />
Organised by [[User:Randogp | Gianpaolo Rando]]<br><br />
<br />
* Main language this week: '''English'''<br><br />
* On parle français, parliamo italiano, falamos portugues<br />
* Theme: '''GMO Detective Workshop'''<br />
* Date: '''Friday 01.06.2018, afternoon''', presentation and discussion start at 19:30<br />
* Location: Room "P1", Hackuarium, 2nd Floor<br />
<br />
Naiane from CRI Paris will join our P1 lab and show the participants how to DNA test if there are GMOs (Genetically Modified Organisms) in their food.<br />
<br />
Bring your food for the test, we bring the pipets and the safety rules.<br />
<br />
While pipetting, we will discuss together about food, DNA, foodtech, GMOs, and participatory science.<br />
<br />
GMO Detective is a low cost and open Citizen Science project for detection of transgenic elements in food and plants.<br />
https://gmodetective.com/<br />
<br />
<br />
RSVP: https://www.meetup.com/hackuarium/events/250778421/<br />
<br />
[[Category:Events]]<br />
[[Category:Workshop]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20180601_GMO_Detective_Workshop_01&diff=1094320180601 GMO Detective Workshop 012018-05-14T17:01:19Z<p>Randogp: Created page with "Everything we eat, everyone we love, sickness and health, the entire living world is written in DNA. Few today understand, but many fear, this new power that is quickly maturi..."</p>
<hr />
<div>Everything we eat, everyone we love, sickness and health, the entire living world is written in DNA. Few today understand, but many fear, this new power that is quickly maturing.<br />
<br />
Organised by [[User:Randogp | Gianpaolo Rando]]<br><br />
<br />
* Main language this week: '''English'''<br><br />
* On parle français, parliamo italiano, falamos portugues<br />
* Theme: '''GMO Detective Workshop'''<br />
* Date: '''Friday 01.06.2018, afternoon''', presentation and discussion start at 19:30<br />
* Location: Room "P1", Hackuarium, 2nd Floor<br />
<br />
Naiane from CRI Paris will join our P1 lab and show the participants how to DNA test if there are GMOs (Genetically Modified Organisms) in their food.<br />
<br />
Bring your food for the test, we bring the pipets and the safety rules.<br />
<br />
While pipetting, we will discuss together about food, DNA, foodtech, GMOs, and participatory science.<br />
<br />
GMO Detective is a low cost and open Citizen Science project for detection of transgenic elements in food and plants.<br />
https://gmodetective.com/<br />
<br />
<br />
RSVP: https://www.meetup.com/hackuarium/events/250778421/</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Main_Page&diff=10942Main Page2018-05-14T16:30:28Z<p>Randogp: /* Upcoming Hackuarium Events */</p>
<hr />
<div>Welcome to the wiki of the [http://www.hackuarium.ch/ Hackuarium] association! <br><br />
<br />
<br />
At Hackuarium, we want to bring biology (and biologists) to the world, and the real world back to biology. Our laboratory is an excuse to meet and discuss, build and develop ideas in a neutral, open, noncompetitive and not-for-profit environment.<br><br />
<br />
This page was launched during our [[1st Ever Hackuarium Wiki Sprint]] that took place on 7 December 2014. As you might notice quickly, almost everything here is a work-in-progress.<br />
<br />
Scroll down and find out what this is all about!<br />
<br />
<div><br />
<big>''[[Hackuarium | Cliquez ici pour accéder à la version française de ce wiki.]]''</big> <br />
</div><br />
<br />
[[File:PosterHackuarium.png |535px|thumb|right|Hackuarium Poster (Not-for-printing version!!! see Section 7.9 [[Main_Page#Visual_Identity | Visual Identity]] for full size printing version)]] <br />
<br />
__TOC__<br />
<br />
<br><br />
<br />
=What is DIYbio?=<br />
DIYbio (Do-It-Yourself biology) is a movement that wants to free the practice of biological research and innovation from the institutional and industrial laboratories. Pursued both by amateurs and professional biologists, DIYbio is developing low-cost and low-tech solutions to problems identified by the community.<br />
<br />
The movement is characterised by an extremely diverse set of practices and participants. Some call themselves 'biohackers', in reference to the hacker culture. The quick solution (even if unconventional) is what is meant by hacks...<br />
<br />
[http://DIYbio.org DIYbio.org] and [http://DIYbio.eu DIYbio.eu] are the portals of the international and european communities, respectively.<br />
<br />
For more information, visit the [http://www.wikipedia.org Wikipedia] page dedicated to [https://en.wikipedia.org/wiki/DIYbio DIYbio].<br><br />
<br />
We actually believe more and more in doing DIT (Do-It-Together) Science, not just 'DIYbio,' as it reflects better the scope of the trans-disciplinary projects we do (often not only biology).<br><br />
<br />
<br><br />
<br />
=What is Hackuarium?=<br />
<br />
Hackuarium is a not-for-profit association aiming at democratizing science through public engagement.<br><br />
<br />
Our laboratory in Renens (Switzerland) is open to anyone sharing the values of the association and who is dedicated to follow the [http://diybio.org/codes/draft-diybio-code-of-ethics-from-european-congress/ DIYbio Europe ethical guidelines].<br><br />
<br />
Our projects are initiated and carried out by scientists and non-scientists from a variety of backgrounds. They are passionate about tinkering with biology in particular, and technology in general. Some are engineers, architects, designers, IT and computer scientists or retired professionals, but others have no scientific education. They are mostly citizens interested in open and participatory research and innovation, outside the constraints of traditional institutions.<br><br />
<br />
Hackuarium members want to investigate new ways of carrying out interdisciplinary research and innovation, by making their results accessible (low-cost), simple and easily reproducible (low-tech) and by promoting an open source philosophy.<br><br />
<br />
<br><br />
<br />
=Where are we?=<br />
<br />
Hackuarium is proudly using infrastructures provided by [http://www.univercite.ch UniverCité], an unconventional innovation hub that opened in May 2014 in the IRL building in Renens. Our equipment is mostly upcycled material from institutions and industries from western Switzerland. We have documented our working environment as it has evolved.<br><br />
<br />
<br />
Between December 2015 and January 2017, UniverCité regrouped our laboratory, a workshop and coworking space.<br><br />
<br />
The original space was under construction work from January until June 2017, and we temporarily relocated on the first floor of the same building.<br><br />
<br />
Since July 2017, we are back on the second floor of what is now called the "Ateliers de Renens" Chemin du Closed 5, 1020 Renens. We have a biohackerspace where (almost) everything goes, and a P1 facility for synthetic bio enthusiasts and more. <br> Check it out during one of our open nights, every Wednesday... <br><br />
<br />
Renens is a hopping hub between Lausanne and Geneva. You can get to us by train from the airport in Geneva within an hour.<br><br />
<br />
<br><br />
<br />
=Who are we?=<br />
<br />
Our members reflect the diversity that is in and around Lausanne/Renens.<br><br />
<br />
[[People | Check us out]], and find out what makes Hackuarium a good mix.<br><br />
<br />
==Committee==<br />
<br />
The current [[Board | committee]] is composed of the following people:<br><br />
<br />
* President: [[User: Rachel | Rachel Aronoff]] (also Citizen Science coordinator and Biosafety Officer)<br />
* Lab Manager: [[User: Gustavo Santamaria| Gustavo Santamaria]]<br />
* Secretary and Treasurer: [[User: luchenry | Luc Henry]]<br />
* Shadow Treasurer: Masoud Alipour<br />
* Deputy Biosafety Officer and Communications Coordinator, and Shadow Secretary: Olivier Emery<br />
* Art and Science coordinator: [[User: Vlorenzo | Vanessa Lorenzo]] <br />
* System Administrator: [[User:Sam | Sam Sulaimanov]]<br />
* Talent scout and evangelist for Academy: [[User:Alpittet | Anne-Laure Pittet]]<br />
* Talent scout and evangelist for Entrepreneurs: [[User:Randogp | Gianpaolo Rando]]<br />
<br />
<br />
The Agenda of the last board meeting can be found [[20180329_Board_Meeting | here]].<br><br />
The Agenda of the next board meeting can be found [[20180523_Board_Meeting | here]].<br><br />
<br />
==Members and Membership==<br />
<br />
'''Do you have a project in mind? Do you just want to support us?'''<br><br />
<br />
Hackuarium operates on a membership basis, even though all events are open to anyone, including non-members.<br><br />
<br />
The monthly membership fee is 20 CHF. It gives 24/7 access to the lab.<br><br />
<br />
We would love to have you as a member! Just follow the instructions below.<br><br />
<br />
===Want to join?===<br />
<br />
* We have a [https://docs.google.com/forms/d/e/1FAIpQLSdoyS8HmsuZACYp-q79pLzxR9BPA60HbyL6ZR8x7h7x3eqbhQ/viewform new form on the website], to fast-track joining the community, if you are keen, perhaps after a few #OpenHackuarium experiences (every Wednesday evening is open to the public).<br />
<br />
* Read some helpful info [[I_m_new | here]].<br />
<br />
* Write an email to hello@hackuarium.ch and give us a short description of:<br />
** Yourself, your interests, your background<br />
** What you would like to learn or do at Hackuarium<br />
<br />
==Friends of Hackuarium==<br />
<br />
Spaces:<br />
::<li>[[UniverCité]]<br />
::<li>[https://onlfait.ch/fablab/ FabLab Onl'fait, Genève]<br />
::<li>[http://lapaillasse.org La Paillasse, Paris]<br />
::<li>[https://lapaillassaone.wordpress.com Paillasse Saône, Lyon]<br />
::<li>[http://foundry.bio The BioFoundry, Sydney]<br />
::<li>[http://www.bioquisitive.org.au Bioquisitive, Melbourne]<br />
<br />
Projects:<br />
::<li>[http://octanis.org Octanis]<br />
::<li>[http://hybridoa.org Hybridoa]<br />
::<li>[http://apéro-sciences.ch/ Apéro Sciences]<br />
::<li>[http://www.genomicintegrity.org AGiR!]<br />
::<li>[[Exodes_Urbains | Exodes Urbains]]<br />
::<li>[http://echopen.org/index.php?title=Main_Page EchOpen]<br />
<br />
<br><br />
<br />
=Events=<br />
<br />
Our lab and space are open to the public. Please come and have a chat! And if you have any questions [mailto:hello@hackuarium.ch get in touch!]<br />
<br />
<big>'''[[Open_Hackuarium | OpenHackuarium]]: Every Wednesday, 19:00-22:00'''</big><br />
<br />
You want to organise an event at Hackuarium, or with the Hackuarium community? We have guidelines below.<br><br />
<br />
==Upcoming Hackuarium Events==<br />
<br><br />
<br />
'''2018'''<br><br />
:<li>'''2018.07.25''' Wednesday 18:30-19:30: [[20180725_Board_Meeting | Board Meeting]]<br />
:<li>'''2018.06.27''' Wednesday 18:30-19:30: [[20180627_Board_Meeting | Board Meeting]]<br />
:<li>'''2018.06.01''' Friday afternoon: [[20180601_GMO_Detective_Workshop_01 | GMO Detective Workshop]] - with Naiane Ribeiro Rios from CRI Paris. Bring your food and discover if it contains GMOs.<br />
:<li>'''2018.05.31''' Thursday 18:30-12:30: Hackuarium will be paying a visit to FabLab Onl'fait and ImpactHub Geneva<br />
:<li>'''2018.05.23''' Wednesday 19:30-22:00: [[20180523_OH203 | Low-cost electronics, DIY, education and development]] - with Govinda Upadhyay, Founder & CEO of LEDsafari<br />
:<li>'''2018.05.23''' Wednesday 18:30-19:30: [[20180523_Board_Meeting | Board Meeting]]<br />
:<li>'''2018.05.19''' Saturday 14:30-18:30: [[20180519_Hack_ton_doudou | Hack ton doudou workshop]] - Our friends from FabLab Onl'fait will teach us doudou hacking!<br />
:<li>'''2018.05.16''' Wednesday 19:30-22:00: [[20180516_OH202 | Swiss Engineers visit Hackuarium]] - Looking forward to an evening of biohacks? Join us!<br />
<br />
<br />
<br><br />
<br />
or follow our [https://calendar.google.com/calendar/embed?src=mgvq9goephpmna0kb7rj9ipei8%40group.calendar.google.com&ctz=Europe/Zurich google calendar]<br><br />
<br />
==Other Upcoming Events at [http://www.univercite.ch UniverCité]==<br />
<br><br />
<br />
==Other Upcoming Events==<br />
:<li>'''2018.03.08''' Thursday 10:30-16:30: [[20180308 Swilabs Meeting Zurich | 20180308 Swilabs Meeting Zurich ]] <br><br />
<br />
==Past Hackuarium Events==<br />
<br><br />
You can find the complete list of our '''past events''' [[past events | here]].<br />
<br />
==Event Organisation== <br />
<br />
You want to organise an event? Go for it!<br><br />
<br />
Help us spread the love for tech, science, art and design.<br><br />
<br />
Here is a "Wiki express" so that everyone has the basics: <br><br />
<br />
*Send an email to board@hackuarium.ch — to make sure there is no conflict with another event<br />
*Someone on the board will check that the space is free<br />
*Build a wiki page for the event (see template below)<br />
*Our 'communications coordinator' will get in touch to ask for content and will make sure the announcements are made on all the social media outlets for Hackuarium!<br />
*In case you need a microgrant to cover the costs (up to 300.-), send an email to board@hackuarium.ch with a description (what, when, who) and a preliminary budget.<br />
*When all this is arrange, talk to the community, to spread the word —> us the slack '''#_uc-events''' channel<br />
*Is it a WORKSHOP? All the previous & make sure you get your logistics right! (and use the tag at the bottom of the page)<br />
*Is there a SIMILAR workshop ALREADY planned? If yes, negotiate dates and: Do you target the SAME people? If yes, negotiate. If not, no problem. It maybe interesting to see what others do.<br />
<br />
===Template for an Hackuarium event===<br />
<br />
First, create a line for your event on the main page under the [Main_Page#Events | Event section] using the following line:<br />
<br />
<pre><br />
:<li>'''201X.XX.XX''' Wednesday 19:00-22:00: [[201XXXXX_TITLE | TITLE]] - SHORT DESCRIPTION<br />
</pre><br />
<br />
Make sure the date, time and title are provided. Save the main page.<br><br />
The event will appear with a link. By clicking on this link you will create the event page.<br><br />
The following must be added to the event page (replace XXX by appropriate values):<br><br />
<br />
'''Header'''<br><br />
<br />
<pre><br />
Open Hackuarium #XX - TITLE<br><br />
Organisé par XXX<br><br />
<br />
'''ENGLISH BELOW''' <br><br />
<br />
* Langue principale cette semaine: '''Anglais'''<br><br />
* Thème : '''TITLE'''<br />
* Date : '''Mercredi XX.XX.2018, 19h''', présentation et discussion à 19:30 et jusqu'à 21h<br />
* Lieu : Salle "XXX", UniverCité, 3ème étage<br />
<br />
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -<br><br />
<br />
* Main language this week: '''English'''<br><br />
* Theme: '''TITLE'''<br />
* Date: '''Wednesday XX.XX.2018, 19:00''', presentation and discussion start at 19:30<br />
* Location: Room "XXX", UniverCité, 3rd Floor<br />
</pre><br />
<br />
'''Main Text'''<br><br />
<br />
Add a text description providing more details about the event.<br><br />
<br />
You include pictures in the same way as in any other page using the following code:<br />
<br />
<pre><br />
[[File:XXX.png|500px]]<br />
</pre><br />
<br />
Add the following tags at the bottom of the page:<br />
<br />
<pre><br />
[[Category:Events]]<br />
[[Category:OpenHackuarium]]<br />
</pre><br />
<br />
And eventually:<br />
<br />
<pre><br />
[[Category:Workshop]]<br />
</pre><br />
<br />
<br><br />
<br />
=Projects=<br />
<br />
Here, you will find the projects going on at Hackuarium.<br />
<br />
== Active Projects ==<br />
<br />
* [[BeerDeCoded]]<br />
* [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Living Instruments]]<br />
* [[Micro to Macro Water Pollution]] - with [http://www.hammerdirt.ch/ Hammerdirt] and [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Spectro-pointer]]<br />
* [[Darty_Monkeys]]<br />
* [[Octanis]] - with more information about their Rover / Balloon [http://octanis.org/rover here]<br />
* [[Grow%26Mix_Bioink]]<br />
* [[AGiR! for genomic integrity]]<br />
* [[Moss Menageries with AGiR!]]<br />
* [[Magnesium tests with AGiR!]]<br />
* [[Urban gardens with rhizobial bacteria]]<br />
* [[Open_Source_Bioreactor | Open Source Bioreactor]]<br />
* [[Bioreactor | Steady state bioreactor]]<br />
* [[Workshop_Bioprinter| Workshop Bioprinter]]<br />
* [[Terraforming Alkalin Experiments | Terraforming Alkaline Experiments]]<br />
* [http://wiki.hackuarium.ch/w/Diy-transilluminator DIY Transilluminator]<br />
<br />
== Archived Projects ==<br />
* [http://wiki.hackuarium.ch/w/Crowdfunding2017 Crowdfunding]<br />
* [[HTGAA | HTGAA]]<br />
* [[Hacktion | Hacktion challenges]]<br />
* [[Open-Food-DNA |Open-Food-DNA]]<br />
* [[Bience | Bience (Beer and Science!)]]<br />
* [[Mycelium Structures]]<br />
* [[Live Type]]<br />
* [[Projects:LivingLab Design|LivingLab Design]]<br />
* [[Projects:bioluminescence|Bioluminescence]]<br />
* [[Projects:Quantitative Anthropology|Quantitative Anthropology]]<br />
* [[Projects:Impression 3D|Impression 3D]]<br />
* [[Projects:Dépollution des stands de tirs Suisses]]<br />
* [[Incubateur bon marché]]<br />
* [[Space Seed]]<br />
* [[Reception d'images satellites]]<br />
* [[Edible wall]]<br />
* [[ZéBU CH]]<br />
* [[Compost Bacteria]]<br />
* [[Protein purification systems]]<br />
* [[unmapping]]<br />
* [[Colab Biomaterials | Colab Biomaterials]]<br />
<br />
<br><br />
<br />
= Practical Information =<br />
<br />
== Rules at Hackuarium ==<br />
<br />
We have very few rules at Hackuarium. The goal of the association is to provide freedom to its members, so they can explore beyond what is allowed elsewhere. However, we want members and anyone participating in our activities to feel comfortable and respected. <br><br />
<br />
[[Life_Main | This page]] describes the code of conduct for Hackuarium members.<br><br />
<br />
== Use of P1 laboratory ==<br />
As of November 2017, Hackuarium is officially hosting its own P1 laboratory. While we still lack some essential infrastructure (-80 freezer, ventilated fume hood), we are working on it... <br />
More can be found out about use of this facility by following the next link...<br />
[[P1_Lab_Activities| P1 Lab Activities]]<br />
<br />
== [[Protocols | Protocols]] ==<br />
All the protocols that we use at Hackuarium will be found [[ BioP_Main | here ]] (work in progress).<br />
They are detailed and structured so that the main focus is on applications. The idea is to give crucial information on how to apply the most common techniques of the Biology toolbox. The protocols are structured as follow:<br />
::<li> A scheme describing each <br />
::<li> A brief description of the protocol and its use<br />
::<li> A description of the different ways of carrying out the protocol. Ideally there should be a description of a commercial kit and a DIY kit.<br />
::<li> A description of the safety issues and best practice<br />
::<li> Information specific to Hackuarium and where to find the components, tools and reagents.<br />
<br />
== Instruments available ==<br />
[[Instru_Main | This page ]] describes all the instruments available at Hackuarium (work in progress..), their location and how to use them.<br />
<br />
=== Up-to-date list of instruments we still need ===<br />
This [https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing on-line document] contains all the instruments, tools and consumables we are looking for. We have a standard donation contract ([[Contrat_de_donation|contrat de donation]], in French) and if your organisation happen to discard a piece of equipment we are (or may be) looking for, [mailto:hello@hackuarium.ch contact us]!<br />
<br />
== Where we shop ==<br />
<br />
As a general rule, we do not buy anything. We try to recycle, upcycle, bicycle, epicycle anything we can, or ask around if anyone can provide supplies for free.<br><br />
<br />
In some cases it does not work out, and so these are the place where we can find what we need.<br><br />
<br />
In any case, '''Please contact [[User:Luchenry|Luc Henry]] if you need to order anything in the name of Hackuarium.'''<br><br />
<br />
===Labware and Reagents===<br />
<br />
Consumables and some chemicals can be purchased from [http://www.huberlab.ch Huber Lab].<br><br />
More chemicals can be obtained from [https://www.carlroth.com/ch/en Carl Roth].<br><br />
If necessary, other chemicals and biochemicals can be obtained from [https://www.sigmaaldrich.com Sigma Aldrich].<br><br />
Molecular biology tools can be purchased from [https://www.qiagen.com/ch/ Qiagen].<br><br />
<br />
Swiss-based [http://www.smiples.ch Smiples] has a very extensive listing of second hand laboratory and technical items. These can be bought from their online shop or from [https://www.ricardo.ch/online-shop/smiples/?SellerNickName=Smiples Ricardo].<br><br />
But most laboratory hardware can be obtained from our network of friends and partners. Get in touch with [[User:Luchenry|Luc Henry]] if you need anything specific and we'll do our best to find it.<br><br />
<br />
===Organic===<br />
Algae, protozoa, acuatic life and educational kits: [https://www.sciento.co.uk/ | Sciento]<br><br />
Bacteria, global biosource center (Hackuarium has a customer account, shop through our Biosecurity manager): [https://www.lgcstandards-atcc.org/?geo_country=ch | ATCC]<br />
<br />
===Hardware===<br />
Options for electronic parts:<br />
<br />
* [http://www.ebay.com Ebay] (international)<br />
* [http://www.aliexpress.com AliExpress] (China)<br />
* [https://www.adafruit.com Adafruit] (UK)<br />
* [http://www.seeedstudio.com/depot/ Seeedstudio] (China)<br />
* [http://www.tmart.com/ Tmart] (Hong Kong)<br />
* [https://www.pi-shop.ch/ Rasberry pi shop] (Switzerland)<br />
* [http://www.distrelec.ch/ Distrelec] (Switzerland)<br />
<br />
==How to built a new project page on our Wiki==<br />
<br />
If you want to start or edit a project page on Hackuarium's wiki, please follow these [[Editing the wiki | instructions]].<br><br />
<br />
== ReadingList ==<br />
<br />
=== The DIY biology / Biohacking movement ===<br />
<br />
<li>[http://delfanti.org/biohackers/ Biohackers. The politics of open science] by Alessandro Delfanti (2013) London: Pluto Press.<br />
::[http://delfanti.org/wp-content/uploads/2013/05/Science-2014-Golinelli-521.pdf A review] by [[User:luchenry | Luc Henry]]<br />
<li>[http://link.springer.com/article/10.1186/s40504-016-0039-1 DIY-Bio – economic, epistemological and ethical implications and ambivalences] by Jozef Keulartz and Henk van den Belt (2016) Life Sciences, Society and Policy<br />
<li>[http://www.biobasedworldnews.com/biohacking-everything-you-need-to-know-about-diy-biology Biohacking] everything you need to know about diy biology, (Accessed 05.2016)<br><br />
<li>[http://www.prnewswire.com/news-releases/genspace-nyc-receives-350000-in-support-from-the-simons-foundation-300237457.html Genspace gets funding], PRNewswire, (Accessed 05.2016)<br><br />
<li>[http://www.nature.com/news/governance-learn-from-diy-biologists-1.19507 Learn from DIY biologists], Todd Kuiken, Nature, (Accessed 05.2016)<br />
<br />
<br><br />
<br><br />
<br />
And a much more complete list by [https://www.kuleuven.be/wieiswie/en/person/00105850 Massimiliano Simons], KU Leuven:<br />
<br />
<br><br />
<br><br />
<br />
<li>[http://www.the-scientist.com/?articles.view/articleNo/34457/title/The-Rebirth-of-DIYbio/ The Rebirth of DIYbio] by Jef Akst, The Scientist, March 2013<br />
<li>[https://www.newscientist.com/article/mg21829236-300-citizen-scientist-out-of-the-lab-and-onto-the-streets/ Out of the lab and onto the streets] by Kat Austen, New Scientist, June 2013<br />
<li>[https://academic.oup.com/bioscience/article/65/1/112/379448/DIYbio-Alternative-Career-Path-for-Biologists DIYbio - Alternative Career Path for Biologists?] by Beth Baker, BioScience (2015) 65 (1): 112<br />
<li>[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1109.html From synthetic biology to biohacking: are we prepared?] by Gaymon Bennett, Nils Gilman, Anthony Stavrianakis & Paul Rabinow, Nature Biotechnology 27, 1109 - 1111 (2009)<br />
<li>[http://repository.jmls.edu/ripl/vol10/iss3/1/Patent Office as Biosecurity Gatekeeper: Fosering Responsible Science and Building Public Trust in DIY Science] by Brian J. Gorman, J. Marshall Rev. Intell. Prop. L. 423 (2011)<br />
<li>[http://wi.mobilities.ca/roberta-buiani-biolab-on-wheels-finding-a-space-for-a-diy-bio-lab-in-toronto/ Biolab-on-Wheels: finding a space for a DIY bio lab in Toronto] by Roberta Buiani, Journal of Mobile Media, February 2015<br />
<li>Charisius, Hanno & Friebe, Richard & Karberg, Sascha - Biohacking: Gentechnik aus der Garage<br />
<li>Curry, Helen Anne - From garden biotech to garage biotech: amateur experimental biology in historical perspective<br />
<li>Davies, Sarah R. & Karin Tybjerg & Louise Whiteley & Thomas Söderqvist - Co-curation as hacking: biohackers in Copenhagen's Medical Museion<br />
<li>Delfanti, Alessandro - Biohackers: The Politics of Open Science<br />
<li>Delfanti, Alessandro - Tweaking genes in your garage: biohacking between activism and entrepreneurship<br />
<li>Delfanti, Alessandro - Is Do-it-Yourself Biology Being Co-opted by Institutions?<br />
<li>Delfanti, Alessandro - Distributed biotechnology<br />
<li>Delfanti, Alessandro - Hacking genomes. The ethics of open and rebel biology<br />
<li>Delgado, Ana - DIYbio: Making things and making futures<br />
<li>Editorial - Empowering citizen scientists<br />
<li>Eggleson, Kathleen - Transatlantic Divergences in Citizen Science Ethics - Comparative Analysis of the DIYbio Code of Ethics Drafts of 2011<br />
<li>Gewin, Virginia - Independent streak<br />
<li>Golinelli, Stefano & Guido Ruivenkamp - Do-it-yourself biology: Action research within the life sciences<br />
<li>Grushkin, D., Kuiken, T., Millet, P - Seven Myths & Realities about Do-It-Yourself Biology<br />
<li>Grushkin, Daniel - Am I a biohazard?<br />
<li>Holloway, Dustin - Regulating Amateurs<br />
<li>Jefferson, Chaterine - Governing Amateur Biology: Extending Respnonsible Research and Innovation in Synthetic Biology to New Actors<br />
<li>Kean, Sam - A Lab of Their Own<br />
<li>Kelty, Christopher - Outlaw, hackers, victorian amateurs: diagnosing publich participation in the life sciences today<br />
<li>Kera, Denisa - Hackerspaces and DIYbio in Asia: connecting science and community with open data, kits and protocols<br />
<li>Kera, Denisa - Innovation regimes based on collaborative and global tinkering: Synthetic biology and nanotechnology in the hackerspaces<br />
<li>Kuiken, Todd - DIYbio: Low Risk, High Potential<br />
<li>Kuiken, Todd - Learn from Do-It-Yourself Biologists<br />
<li>Kuznetsov, Stacey - Expanding Our Visions of Citizen Science<br />
<li>Kuznetsov, Stacey & Alex Taylor & Tim Regan & Nicolas Villar & Eric Paulos - At the seams: DIYbio and opportunities for HCI<br />
<li>Kuznetsov, Stacey & Carrie Doonan & Nathan Wilson & Swarna Mohan & Scott E. Hudson & Eric Paulson - DIYbio Things: Open Source Biology Tools as Platforms fo rHybrid Knowledge Production and Scientific Participation<br />
<li>Landrain, Thomas & Meyer, Morgan & Perez, Ariel Martin & Sussan, Remi - Do-it-yourself biology: challenges and promises for an open science and technology movement<br />
<li>Lisa Z. Scheifele & Thomas Burkett - The First Three Years of a Community Lab: Lessons Learned and Ways Forward<br />
<li>McKenna, Phil - Rise of the garage genome hackers<br />
<li>Meyer, Morgan - Build your own lab<br />
<li>Meyer, Morgan - Domesticating and democratizing science: a geography of do-it-yourself biology<br />
<li>Meyer, Morgan - Hacking Life? The Politics and Poetics of DIY Biology<br />
<li>Meyer, Morgan - Bricoler, domestiquer et contourner la science<br />
<li>Nascimento, Susana & Angela Guimaraes Pereira & Alessia Ghezzi - From Citizen Science to Do It Yourself Science<br />
<li>NSABB - Strategies to Educate Amateur Biologists and Scientists in Non-life Science Disciplines About Dual use Research in the Life Science<br />
<li>Schmidt, Markus - Diffusion of synthetic biology<br />
<li>Scudellari, Megan - Biology Hacklabs<br />
<li>Seyfried, Günter & Pei, Lei & Schmidt, Markus - European do-it-yourself (DIY) biology: Beyond the hope, hype and horror<br />
<li>Sholette, Gregory - Disciplining the avant-garde: The United States versus the Critical Art Ensemble<br />
<li>Sipra Bihani & Michael Hartman & Florian Sobiegalla & Amanda rosenberg - Comparing network strutures of commercial and non-commercial biohacking online-communities<br />
<li>Söderberg, Johan & Delfanti, Alessandro - Hacking Hacked! The Life Cycles of Digital Innovation<br />
<li>Söderberg, Johan & Delfanti, Alessandro - Repurposing the hacker. Three temporalities of recuperation<br />
<li>Tocchetti, Sara - DIYbiologists as 'Makers' of Personal Biologies<br />
<li>Tocchetti, Sara - What kind of work we are doing now and what kind of work we want to do<br />
<li>Tocchetti, Sara & Sara Angeli Aguiton - Is an FBI Agent an DIY Biologist Like Any Oter? A Cultural Analysis of a Biosecurity Risk<br />
<li>Trojok, Rüdiger - Biohacking: Gentechnologie für Alle<br />
<li>van Boheemen, Pieter & Huib de Vriend - Do-it-yourself biology: Een verkenning van ontwikkelingen in Nederland<br />
<li>Wohlsen, Marcus - Biopunk: Solving Biotech's Biggest Problems in Kitchens and Garages<br />
<br />
=== Open Hardware ===<br />
<li>[http://www.nature.com/news/open-hardware-pioneers-push-for-low-cost-lab-kit-1.19518 Open-hardware, pioneers push for low-cost lab kit] by Elizabeth Gibney, Nature, (Accessed 05.2016)<br><br />
<li>[http://www.appropedia.org/Open-source_Lab#Examples Appropedia] a source of DIY instruments builds, Dr. Joshua Pearce, (Accessed 05.2016)<br><br />
<li>[http://www.ohwr.org/projects/fr_pcb_mm The PCB milling machine], Open Hardware Repository, (Accessed 05.2016)<br><br />
:: [https://pt.wikiversity.org/wiki/Pesquisa:Ferramentas_livres:Work_group_for_development_of_the_hyperobject_workbench More]<br />
<li>http://www.creativeapplications.net/processing/sensortape-3d-aware-dense-sensor-network-on-a-tape/, Sensory network on a roll of tape, Filip Visnjic, (Accessed 05.2016)<br><br />
<li>[http://oceanoptics.com/product/redeye-oxygen-sensing-patches/ RedEye Oxygen Sensing Patches], Ocean Optics, (Accessed 05.2016) <br><br />
<br><br />
<br />
=== Art ===<br />
<li> [http://www.booooooom.com/2016/04/04/mattia-menchetti-gives-wasps-coloured-paper-to-create-rainbow-nests/#more-78906 Rainbow wasp nests], Mattia Menchetti, (Accessed 05.2016)<br><br />
<li> [http://thecreatorsproject.vice.com/blog/dirty-beats-hear-music-generated-from-bacteria Dirty beats] making music with bacteria, Interspecifics(Accessed 05.2016)<br><br />
::[http://www.interspecifics.cc/-/ More1], [http://www.ncbi.nlm.nih.gov/pubmed/21239171, More2], [http://www.ncbi.nlm.nih.gov/pubmed/12501293, More3]<br><br />
<br><br />
<br />
=== Ideas/Concepts/Science ===<br />
<li>[http://ideas.ted.com/a-newly-drawn-tree-of-life-reminds-us-to-question-what-we-know/ The new tree of life] TED (Accessed 05.2016)<br><br />
<li>[https://medium.com/now-of-work/the-workplace-of-the-future-work-of-today-16c58df1fa29#.zhbu0c57f Rethinking work], Yann Heurtaux, (Accessed 05.2016)<br><br />
<li>[https://www.kickstarter.com/projects/910418035/plastic-bottle-cutter That plastic bottle cutter], Pavel & Ian, (Accessed 05.2016)<br><br />
<br><br />
<br />
=== Open Source ===<br />
<li>[https://blogs.openaire.eu/?p=882 Results held hostage], Francesco Mondada, (Accessed 05.2016)<br><br />
<br><br />
<br />
==Visual Identity==<br />
* [[Logo]]<br />
* [http://wiki.hackuarium.ch/images/9/9d/HackuariumPoster.pdf Our poster in pdf ready to print]<br />
* Working on a footage for videos (comming soon)<br />
<br />
== Media and Press ==<br />
Please check our [[Press kit]] out. It includes a prototype Press review of most of our media appearances over the years.<br />
<br />
== EU Paperwork ==<br />
We now have an identification number aka [https://ec.europa.eu/research/participants/portal/desktop/en/support/faqs/faq-1055.html Participant Identification Code (PIC)] from the European Participant Portal for any Horizon 2020 application. The Hackuarium PIC number is 907732306. It can be verified on [http://ec.europa.eu/research/participants/portal/desktop/en/organisations/register.html# the official search engine].<br />
<br />
== Wanted ==<br />
Do you want to contribute to making Hackuarium a diverse and lively community? Check out our wanted lists:<br />
::<li>Skills<br />
::<li>Ideas<br />
::<li>[https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing Equipment]<br />
::<li>Consumables<br />
<br />
<br><br></div>Randogphttps://wiki.hackuarium.ch/index.php?title=Past_events&diff=10941Past events2018-05-14T16:30:15Z<p>Randogp: /* Past Events at Hackuarium */</p>
<hr />
<div>__TOC__<br />
== Past Events at Hackuarium ==<br />
<br><br />
<br />
'''2018'''<br><br />
:<li>'''2018.05.09''' Wednesday 19:30-22:00: [[20180516_OH201 | BioSensors - when FabLab meets WetLab]] - Tamara Milosevic tells us about her Parisian project-based course!<br />
:<li>'''2018.05.05''' Saturday 09:30-19:30: [[20180505_Workshop_spectrophotometer | Workshop]] - Build your own spectrophotometer!<br />
:<li>'''2018.05.02''' Wednesday 19:30-22:00: [[20180502_OH200 | 200 weeks of Hackuarium]] - We celebrate with a film night!<br />
:<li>'''2018.04.27''' Friday 18:00-22:00: Visit of Hackuarium by students from CRI Paris<br />
:<li>'''2018.04.25''' Wednesday 19:30-22:00: [[20180425_OH199 | OH199 Open Hackuarium]] - Octanis Update and more<br />
:<li>'''2018.04.21''' Saturday 14:00-17:30: [[20180331_Workshop_DNAdamage | DNA Damage Workshop]] - Open-sourcing DNA damage detection for Citizen Science<br />
:<li>'''2018.04.18''' Wednesday 19:30-22:00: [[20180418_OH198 | Bernard Dugas - Suivant les pas de Pierre Delbet: Magnesium et Santé]] - <br />
:<li>'''2018.04.04''' Wednesday 19:30-22:00: [[20180404_Open Hackuarium_Workshop_microfluidics | OH196 Open Hackuarium]] - Workshop: DIY Microfluidics<br />
:<li>'''2018.03.29''' Thursday 19:00-21:00: [[20180329_Board_Meeting | Board Meeting]]<br />
:<li>'''2018.03.28''' Wednesday 19:00-21:00: [[20180328_OH195 | OH195 Open Hackuarium]] - A special night about Phage Therapy<br />
:<li>'''2018.03.21''' Wednesday 19:00-22:00: [[20180321_OH194_Hackahealth | 20180321 OH194 Hackahealth]] - Students from Campus Biotech are visiting Hackuarium and organising the Hackahealth<br />
:<li>'''2018.03.10''' Saturday 09:30-18:00: [[20180310_Workshop_spectrophotometer | Workshop]] - Build your own spectrophotometer!<br />
:<li>'''2018.03.07''' Wednesday 19:30-22:00: [[20180307_OH192 | General Assembly]] - Annual meeting of the members (all visitors welcome!)<br />
:<li>'''2018.02.28''' Wednesday 19:00-22:00: [[20180228_OH191 | EPFL iGEM is visiting Hackuarium!]]<br />
:<li>'''2018.02.15''' Thursday 19:00-22:00: [[20180215_Visit_LucyPatterson | Everything you always wanted to know about DIY science advocacy]] by Lucy Patterson.<br />
:<li>'''2018.02.14''' Wednesday 19:00-22:00: [[20180214_OH189 | OH189 Business as usual]] We'll be open to visitors! Come meet us.<br />
:<li>'''2018.02.08''' Thursday 19:00-21:00: [[20180208_Board_Meeting | Board Meeting]]<br />
:<li>'''2018.01.18''' Thursday 19:00-21:00: [[20180118_Board_Meeting | Board Meeting]]<br />
<br />
'''2017'''<br><br />
:<li>'''2017.12.02''' Saturday 18:30-22:30: [[Fondue_Night_2017 | Fondue Night 2017]]. The now traditional Fondue Night will be a fantastic opportunity to eat, meet and discuss the future of Hackuarium, in preparation of the [[20180307_OH192 | General Assembly]] that will happen on March 7th 2018.<br />
:<li>'''2017.12.06''' Wednesday 18:00-20:00: [[20171206_Board_Meeting | Board Meeting]]<br />
:<li>'''2017.11.29''' Wednesday 19:00-22:00: [[20171129_AlienatedLandscapes | Open Hackuarium #178 - Alienated Landscapes]]<br />
:<li>'''2017.11.01''' Wednesday 18:30-20:30: [[20171101_Board_Meeting | Board Meeting]]<br />
:<li>'''2017.10.18''' Wednesday 19:00-22:00: [[20171018_CrowdfundingMeeting | Crowdfunding meeting]]<br />
:<li>'''2017.10.04''' Wednesday 18:30-20:30: [[20171004_Board_Meeting | Board Meeting]]<br />
:<li>'''2017.09.27''' Wednesday 19:30-22:30: [[20170927_FilmMaking | Making a Film for Crowdfunding]]<br />
:<li>'''2017.09.13''' Wednesday 19:30-22:30: [[20170913_CrowdfundingMeeting | Crowdfunding meeting]]<br />
:<li>'''2017.09.11''' Wednesday 19:30-22:30: [[20170911_Board_Meeting | Board Meeting]]<br />
:<li>'''2017.08.30''' Wednesday 19:30-22:30: [[20170830_PaPiPoPiPouPou |3rd yr burzday ]]<br />
:<li>'''2017.07.19''' Wednesday 19:30-22:00: [[20170719_WDDNAGotCash | Hey WDDNA waddup?]]<br />
:<li>'''2017.07.12''' Wednesday 19:30-22:00: [[20170712_1cellAtTime |Biology, one cell at the time]]<br />
:<li>'''2017.07.12''' Wednesday 18:30-19:30: [[20170712_Board Meeting | Board meeting]]<br />
:<li>'''2017.07.06''' Thursday 19:00-22:00: [[20170706_BrnstrmCF |Brainstorm the crowdfunding campaign]]<br />
:<li>'''2017.06.27''' Tuesday 09:00-15:00: Inartis Official opening of the 2nd floor REDUX! Sign up [http://univercite.inartis.ch here]!<br />
:<li>'''2017.06.24''' Saturday 10:00-18:00: [[Déménagement, Moving that lab where it belongs! | Déménagement, Moving that lab where it belongs!]] -The lab is moving back to the 2nd floor!<br />
:<li>'''2017.06.14''' Wednesday 19:30-21:00: [[20170614_OH154 | Dessine moi un Hackuarium vol.2]] - We need to plan our next move to the 2nd floor. Come design the new lab!<br />
:<li>'''2017.06.07''' Wednesday 19:30-21:00: [[20170607_Board Meeting | Board meeting]] (in place of [[20170608_Board Meeting | this one]])<br />
:<li>'''2017.06.08''' Thursday 15:00-16:00: [[080617 Meeting Ferme Des Tilleuls | @hello LFdT ]]<br />
:<li>'''2017.05.31''' Wednesday 19:30-21:00: [[201705317_OH152 | Update on micro-droplet based high throughput cell selection]]<br />
:<li>'''2017.05.17''' Wednesday 19:30-21:00: [[20170517_OH150 | Extraordinary General Assembly]]<br />
:<li>'''2017.05.10-14''' Wednesday-Sunday: The [http://citizensciences.net/biofabbing/ Biofabbing] conference will take place in Geneva. Hackuarium will contribute to the Uncoference part: [[Hackuarium visit | Hackuarium visit]], [[Hackuarium Satellite | Hackuarium Satellite]].<br />
:<li>'''2017.05.10''' Wednesday 19:30-21:00: [[20170517_OH149 | On Food Waste]]<br />
:<li>'''2017.05.05''' Wednesday 9:30-11:00: [[20170505_Crowdfunding_talk | Discussing the crowdfunding campaign]]<br />
:<li>'''2017.05.03''' Wednesday 19:00-22:00: [[20170503_OH148 | Open Hackuarium vol. 148 : WWF 'Swiss Litter Report’ Project presentation & Update Montreux Clean Beach Project]]<br />
:<li>'''2017.04.26''' Wednesday 19:00-22:00: [[20170426_OH147 | '''CANCELLED''' Open Hackuarium vol. 147 : Enclosed bacterial culture]]<br />
:<li>'''2017.04.19''' Wednesday 19:00-22:00: [[20170419_OH146 | Open Hackuarium vol. 146 : WDDNA Update]]<br />
:<li>'''2017.04.12''' Wednesday 20:00-22:00: [[20170412_Board_meeting | Board meeting]]<br />
:<li>'''2017.03.29''' Wednesday 19:00-22:30: [[20170329_OH143 | Open Hackuarium vol. 143]] - Bee Hive Monitoring<br />
:<li>'''2017.03.22''' Wednesday 19:00-22:00: [[20170329_OH142 | Open Hackuarium vol. 142]] - Visit of MassChallenge space, 3rd floor<br />
:<li>'''2017.03.15''' Wednesday 19:00-22:00: [[20170329_OH141 | Open Hackuarium vol. 141]] - Git Workshop for those interested! <br />
:<li>'''2017.03.11''' Saturday 09:00-16:00: [[20170311_WikiSpr_Git | GitHub Hands-On & Wiki Sprint]]<br />
:<li>'''2017.03.09''' Thursday 19:00-20:00: [[20170309_Board_meeting | Board meeting]]<br />
:<li>'''2017.03.01''' Wednesday 19:30-22:30: [[20170301_OH139 | Open Hackuarium vol. 139]] - What's Hackuarium again?<br />
:<li>'''2017.02.25''' Saturday 09:00-16:00: [[20170225_Arduino_WS | Octanis Arduino Workshop]]<br />
:<li>'''2017.02.22''' Wednesday 19:30-22:30: [[20170222_WhatsUpOctanis | Whats Up Octanis]]<br />
:<li>'''2017.02.15''' Wednesday 18:00-19:30: [[20170215_Board_meeting | Board meeting]]<br />
:<li>'''2017.02.15''' Wednesday 10:00-18:00: [[20170215_Chromatographies | Chromatography Swiss/Romanian connection]]<br />
:<li>'''2017.02.08''' Wednesday 19:30-21:30: [[Openhackuarium_vol._136 | General Assembly]]<br />
:<li>'''2017.02.01''' Wednesday 19:30-20:30: [[20170201_Board_meeting | Board meeting]]<br />
:<li>'''2017.01.07''' Saturday 9:30-18:30: We're moving! Come join us and help us move the lab to the floor below.<br />
<br />
'''2016'''<br><br />
:<li>'''2016.12.14''' Wednesday 19:30-20:30: [[Openhackuarium_vol._128 | Fondue Night]]. In preparation of the [[Openhackuarium_vol._136 | General Assembly]] that will happen on Feb 8th 2017, we will have a discussion followed by a fondue! All members welcome to talk about the future!<br />
:<li>'''2016.12.09-11''' Friday-Sunday 9:30-18:30: [http://openscienceschool.org/about/co-lab-epfl/ Co-Lab Biomaterial] at EPFL & Hackuarium.<br />
:<li>'''2016.12.07''' Wednesday 19:30-21:30: [[Openhackuarium_vol._127 | Presentation of Co-Lab]]. It is not too late to fin out what will happen at the [http://openscienceschool.org/about/co-lab-epfl/ Co-Lab Biomaterial] workshop on 09-11 December 2016.<br />
:<li>'''2016.11.28''' Thursday 19:30-20:30: [[20161128_Board_meeting | Board meeting]]<br />
:<li>'''2016.11.25-27''' Friday-Sunday 9:30-18:30: [[Science_Hackathon_#SHUC2016 | Science Hackathon 2016]] The first ever Science Hackathon at UniverCité: let's hack research!<br />
:<li>'''2016.11.19-20''' Saturday-Sunday [http://lauzhack.com Lauzhack] at EPFL is organised by CLIC, the Computer & Communication Sciences students' association.<br />
:<li>'''2016.11.19''' Saturday 9:30-18:30: [http://www.urbanentrepreneurs.io/lausanne Lift Urban Entrepreneur] Lift is organising one of their #urbanentrepreneurs events at UniverCité.<br />
:<li>'''2016.11.17''' Thursday 19:30-22:00: [[Openhackuarium_vol._124 | Openhackuarium vol. 124]] "OpenUC, soirée spéciale Makerspace"<br />
:<li>'''2016.11.02''' Wednesday 19:30-22:00: [[Openhackuarium_vol._122 | Openhackuarium vol. 122]] Epidemium and Echopen: when Open Source meets Pharma and Medtech<br />
:<li>'''2016.10.24''' Monday 19:30-20:30: [[20161024_Board_meeting | Board meeting]]<br />
:<li>'''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]] "Dessine moi un UC"<br />
:<li>'''2016.10.02''' Sunday 10:00-18:00: [[OP_Greenhouse | OP Greenhouse]] building the first P1 iteration<br />
:<li>'''2016.10.01''' Saturday 11:30-18:30: [[Balloon_BBQ | Balloon Launch and BBQ]] CANCELLED due to bad weather<br />
:<li>'''2016.09.21''' Wednesday 19:30-22:00: [[Openhackuarium_vol._116 | Openhackuarium vol. 116]] Hackuarium for dummies (and life science students) + Apéromix from 8.30pm!<br />
:<li>'''2016.09.19''' Monday 19:30-20:30: [[20160919_Board_meeting | Board meeting]]<br />
:<li>'''2016.09.14''' Wednesday 19:30-22:00: [[Openhackuarium_vol._115 | Openhackuarium vol. 115]] IntelliGene: EPFL iGEM team wants to build tools for easy synthetic genetic circuit generation<br />
:<li>'''2016.09.10''' Saturday 9:30-18:30: [[Raspberry_Pi_workshop | Raspberry Pi Workshop]]: come test and build with the latest open source computer technology<br />
:<li>'''2016.09.07''' Wednesday 19:30-22:00: [[Openhackuarium_vol._114 | Openhackuarium vol. 114]] Thymio: an open source educational robot<br />
:<li>'''2016.08.31''' Wednesday 19:30-22:00: [[Openhackuarium_vol._113 | Openhackuarium vol. 113]] Fuels from plastics: a DIY approach to recycling<br />
:<li>'''2016.08.24''' Wednesday 19:30-22:00: [[Openhackuarium_vol._112 | Openhackuarium vol. 112]] SimplicityBio, Open mic to community projects<br />
:<li>'''2016.08.18''' Thursday 19:00-22:00: [[20160818_Board_meeting | Board meeting]]<br />
:<li>'''2016.08.17''' Wednesday 19:30-22:00: [[Openhackuarium_vol._111 | Openhackuarium vol. 111]] LoRa & The Things Network: Opening the Internet Of Things to everyone<br />
:<li>'''2016.08.13-15''' Friday-Sunday: [[Living Instruments]] was featured at the [http://www.internationales-musikinstitut.de/en/ International Summer Course for New Music] in Darmstadt, Germany<br />
:<li>'''2016.08.03''' Wednesday 19:30-22:00: [[Openhackuarium_vol._109 | Openhackuarium vol. 109]] Presentation of Atelier6 (Renens) and PatchWork (Lausanne) creative communities.<br />
:<li>'''2016.07.20''' Wednesday 19:30-22:00: [[Openhackuarium_vol._107 | Openhackuarium vol. 107]] Pole2Pole: A Mike Horn adventure feat. BangBang agency (and Hackuarium?).<br />
:<li>'''2016.07.13''' Wednesday 20:00-20:30: [[20160713_Board_meeting | Board meeting]]<br />
:<li>'''2016.07.06''' Wednesday 19:30-22:00: [[Openhackuarium_vol._105 | Openhackuarium vol. 105]] Presentation of Pangoss Labs and the future Makeshop Geneva<br />
:<li>'''2016.06.29''' Wednesday 19:30-22:00: [[Openhackuarium_vol._104 | Openhackuarium vol. 104]] DIYbio: an academic perspective, followed by Camera Obscura<br />
:<li>'''2016.06.23''' Thursday 13:45-16:00, Bern: [[20160623_Citizen_Science_Netzwerktreffen | Citizen Science Netzwerktreffen / rencontre du réseau sciences citoyennes]]<br />
:<li>'''2016.06.18''' Saturday 09:00-18:00 [[ Gorilla_Warfare_II | Gorilla Warefare II]] (followed by a barbecue).<br />
:<li>'''2016.06.09''' Thursday 20:00-21:30: [[20160609_Board_meeting | Board meeting]]<br />
:<li>'''2016.06.01''' Wednesday 19:00-22:00: [[Openhackuarium_vol._100 | Openhackuarium vol. 100]] Hackuarium is celebrating two years of (bio)hacking at UniverCité!<br />
:<li>'''2016.05.11''' Wednesday 19:30-21:00: Openhackuarium vol. 97 Pierre Rogé will present TECH-SAVE, a non-profit foundation which aims to revalue technical intangible Heritage.<br />
:<li>'''2016.04.27''' Wednesday 19:30-21:00: [[Openhackuarium_vol._95 | Openhackuarium vol. 95]] T-shirt printing session. POSTPONED!<br />
:<li>'''2016.04.13''' Wednesday 19:30-21:00: [[Openhackuarium_vol._93 | Openhackuarium vol. 93]] 'Genomic integrity' and DIY 'comet assay' on cheek cells.<br />
:<li>'''2016.04.06''' Wednesday 19:30-21:00: [http://wiki.hackuarium.ch/w/Openhackuarium_vol._92 Openhackuarium vol. 92] Darty Monkeys: 'Biofilia Night'.<br />
:<li>'''2016.03.29''' Tuesday 19:30-21:00: [[20160329_Board_meeting | Board meeting]]<br />
:<li>'''2016.03.09''' Wednesday 19:30-21:30 Openhackuarium vol. 88 Presentation by and brainstorming with [http://www.hammerdirt.ch/current-project.html Hammerdirt]<br />
:<li>'''2016.03.08''' Tuesday 16:50 [[Events:Entre_Bière_Hackers_et_ADN_Café_Scientifique_au_Collège_Rousseau |Entre bière, hackers et ADN]] The [[BeerDeCoded| BeerDeCoded project]] on stage at the Café Scientifique of Collège Rousseau (Geneva).<br />
:<li>'''2016.03.04''' Friday 19:30-22:30 Visite of Hackuarium by participants of the [[GOSH_meeting | Gathering for Open Science Hardware]] (aka [http://openhardware.science GOSH!]).<br />
:<li>'''2016.02.12''' Friday 9:00-11:00: The [[Living_Instruments| Living Instruments]] project will be featured as part of the official [http://liftconference.com/lift16/workshops/living-organisms-musical-instruments-unprecedented-performance Lift Conference] program.<br />
:<li>'''2016.02.10''' Wednesday 19:30-21:00: School trip to the music venue [http://www.le-bourg.ch le Bourg] for an [http://www.wespoke.ch/les-concerts/le-bourg-2-hackuarium/ Openhackuarium vol. 84 special music] featuring the [[Living_Instruments| Living Instruments]] project.<br />
:<li>'''2016.02.04''' Thursday 19:30-20:30 [[20160204_Board_meeting | Board meeting]]<br />
:<li>'''2016.02.03''' Wednesday 19:30-21:00 Participants to the Biodesign.cc [http://wiki.biodesign.cc/wiki/Winter_School Winterschool] visit [[Openhackuarium_vol._83 | Openhackuarium vol. 83]].<br />
:<li>'''2016.01.23-24''' Saturday and Sunday 09:00-19:00 Another week-end dedicated to biomusical innovation with the [[Living_Instruments| Living Instruments]] project<br />
:<li>'''2016.01.20''' Wednesday 19:30-20:30: [[20160120_General_Assembly | General Assembly]] of the Hackuarium association. Please come, as a member, or as a friend!<br />
<br />
'''2015'''<br><br />
<br />
:<li>'''2015.12.09''' Wednesday 19:30-21:00: [[Openhackuarium_vol._75 | Openhackuarium vol. 75]] with a presentation of the project beMap.<br />
:<li>'''2015.12.02''' Wednesday 19:30-21:00: [[Openhackuarium_vol._74 | Openhackuarium vol. 74]] with presentations about open source projects in protein science and more!<br />
:<li>'''2015.11.28''' Saturday 9:30-15:30: [[DNA_Party_workchoppe_vol.2 | The real Beer DNA workshop]] Sign-up! Limited space available.<br />
:<li>'''2015.11.26''' Jeudi 19:30-21:00: [[20151128_Board_Meeting | Board Meeting]]<br />
:<li>'''2015.11.05''' Thursday 19:00-22:00: Lausanne Open Data vol. 3 [[Data_sharing_-_Lausanne_Open_Data_Meetup|Special meetup on Biomedical Data & Ethics]] ([http://www.meetup.com/Lausanne-Open-Data-Meetup/events/223791979/| more here])<br />
:<li>'''2015.10.28''' Wednesday 19:30-23:00: [[Openhackuarium_vol._69 |#OpenHackuarium vol. 60]] [http://hackteria.org Hackteria]: DIY bio in Indonesia and the Biology of cheese and wine. Part of a week-long experiment with [http://www.hesge.ch/head/en HEAD]: [http://hackteria.org/wiki/The_Art_of_BioHacking_-_HEAD The Art of BioHacking]<br />
:<li>'''2015.09.19-21''' A long week-end dedicated to biomusical innovation with the [[Living_Instruments| Living Instruments]] project<br />
:<li>'''2015.08.27''' Thursday 19:00-22:00: Lausanne Open Data vol. 1 ([http://www.meetup.com/Lausanne-Open-Data-Meetup/events/223762682/| more here])<br />
:<li>'''2015.08.26''' Wednesday 19:30-20:30: [[Openhackuarium_vol._60_%22Lac_Léman_Science_Hackathon%22_260815|#OpenHackuarium vol. 60: Do we need Lac Léman Science Hackathon?]]<br />
:<li>'''2015.08.20''' Thursday 19:00-22:00: Drone Racing 101<br />
:<li>'''2015.08.05''' Wednesday 19:30-20:30: [[Openhackuarium_vol._57_%22Bi%C3%A8re%22 | #OpenHackuarium vol. 57]] special Fermentation.<br />
:<li>'''2015.07.22''' Wednesday 19:30-20:30: [[Openhackuarium_vol._55_-_22.07.15 | #OpenHackuarium vol. 55]] special Urban/Vertical farming.<br />
:<li>'''2015.07.18''' Saturday 9:30-15:30: [[DNA_Party_workchoppe_vol.1 | A first DNA workshop/workchoppe]].<br />
:<li>'''2015.04.22''' Wednesday 19:30-20:30: General Assembly of Hackuarium association.<br />
:<li>'''2015.04.16''' Thursday 19:00-21:00: Discussion with Le Pansement, a medical students association.<br />
:<li>'''2015.04.15''' Wednesday 18:30-21:00: #OpenHackuarium vol. 41 Practical Session "Garage Electrophoresis" with Le Pansement, a medical students association.<br />
:<li>'''2015.03.29''' Sunday 13:30-18:30: [[2nd_Hackuarium_Wiki_Sprint | Hackuarium Wiki Sprint #2]].<br />
:<li>'''2015.03.28''' Saturday 10:00-18:30: [[Open_DNA_Seq_Workshop | Open DNA Sequencing Workshop]].<br />
:<li>'''2015.02.18''' Wednesday 19:30-21:30: #OpenHackuarium vol. 33 [[Visite_du_FlyLab_Paris_chez_nous| Drone night]] with Flylab Paris (part of La Paillasse) (FR).<br />
<br />
'''2014'''<br><br />
<br />
:<li>'''2014.12.07''' [[1st_Ever_Hackuarium_Wiki_Sprint | Hackuarium Wiki Sprint #1]].<br />
:<li>'''2014.11.27''' [[Events:BSNL_Roundtable | Roundtable at EPFL organised by BNSL]].<br />
:<li>'''2014.11.05''' [[Events:Bioart_NexTrends | NexTrends/ECAL Bioart conference]] at UniverCité.<br />
:<li>'''2014.10.22''' Wednesday 19:30-21:30: #OpenHackuarium vol. 16 [[Events:Open_Access | Open Access conference]] at UniverCité.<br />
:<li>'''2014.10.09''' Thursday 19:30-21:30: Conference by French artist [http://liagiraud.com Lia Giraud].<br />
:<li>'''2014.09.25-27''' [[Events:Design_Days_2014 | Design Days 2014]].<br />
:<li>'''2014.09.24''' Wednesday 19:30-21:30: #OpenHackuarium vol. 12 Conference by [http://www.nicolasnova.net Nicolas Nova], co-fondateur du [http://nearfuturelaboratory.com Near Future Laboratory] et enseignant à la [http://www.hesge.ch/head/ HEAD], Genève.<br />
:<li>'''2014.08.27''' Wednesday 19:30-21:30: #OpenHackuarium vol. 8 Introduction to [http://bricobio.org BricoBio] Montreal by Sarah Choukah.<br />
:<li>'''2014.08.20''' Wednesday 19:30-21:30: #OpenHackuarium vol. 7 Introduction to [http://lapaillasse.org La Paillasse] Paris by Clément Epié and Nicolas Loubet.<br />
:<li>'''2014.08.13''' Wednesday 19:30-21:30: [[Openhackuarium_vol._6 | General Assembly]]<br />
:<li>'''2014.06.06-07''' [http://hackteria.org/wiki/Tech4Dev Water Hackathon] organised by Hackteria.<br />
<br />
== Participation to External Events ==<br />
<br />
'''2017'''<br />
:<li>'''2017.11.01''' Thursday 10:30-16:30: [[SWILABS_3rd_MEETING| SWILABS 3rd MEETING in Lugano]]<br />
:<li>'''2017.02.11 - 2017.03.11''' Exhibition [http://utopiana.ch/la-semiosphere-du-commun/ La sémiosphère du Commun], Espaces d’exposition «Le Commun», Bâtiment d’Art Contemporain (BAC), Genève. Works by Vanessa Lorenzo, Guillaume de Morsier and others.<br />
<br />
'''2016'''<br />
<br />
'''2015'''<br />
<br />
'''2014'''<br />
<br />
::<li>'''2014.11 - 2014.12''' [http://www.gasthauszumbaeren.ch//wire-lab/re-thinking-food- Re-Thinking Food], [http://www.gasthauszumbaeren.ch/de/home Gasthaus zum Bären], [http://www.thewire.ch/en/ W.I.R.E.], Zurich, Switzerland<br />
::<li>'''2014.09.25 - 2015.02.22''' [http://www.museedelamain.ch LAB/LIFE Exhibition], Musée de la Main, Lausanne, Switzerland</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20180118_Board_Meeting&diff=1019620180118 Board Meeting2018-01-09T15:10:29Z<p>Randogp: </p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Thursday, January 18th, 19.00-21.00pm<br />
<br><br />
Place: UniverCité, 3rd floor "Salle Tournesol" meeting room<br />
<br />
Everyone is again invited to this Board Meeting, as these are such exciting times!<br />
<br />
__TOC__<br />
<br><br />
<br />
=Chairs=<br />
* Vanessa et Rachel<br />
<br />
==Present==<br />
* Luc<br />
<br />
==Excused==<br />
* <br />
<br />
==Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br><br />
<br />
QUESTION: Are there any objections?<br><br />
*Yes/No<br />
<br />
Time keeping<br><br />
QUESTION: WHO?<br><br />
*NAME<br />
<br />
=Items=<br />
<br />
==Point 1: Accounting 2017==<br />
(Luc, 5min)<br />
<br />
* Reminder that the treasurer needs ALL receipts for transactions. Alternatively, an invoice can be produced (but no need for both).<br />
* Summary of 2017 finances and how they will be presented at the [[20180307_OH192 | General Assembly]].<br />
<br />
QUESTION: does the board validate the reporting as it is, and let Luc present, and the "vérificateurs de comptes" report?<br />
*Yes/No<br />
<br />
==Point 2: [[20180307_OH192 | General Assembly]] 2018==<br />
(Luc, 5min)<br />
<br />
*Reminder of the role of the General Assembly<br />
<br />
QUESTION: what specifically do we want to present/discuss this year?<br />
*ANSWER<br />
<br />
==Point 3: ==<br />
(Luc, 5min)<br />
<br />
<br />
QUESTION: does the board validate the reporting as it is, and let Luc present, and the "vérificateurs de comptes" report?<br />
*Yes/No<br />
<br />
==Point 2: Crowdfunding==<br />
(Rachel, 0min)<br />
<br />
===Perk wrapup===<br />
<br />
Luc: PLEASE DESCRIBE HERE IN 2 SENTENCES.. NO NEED TO DISCUSS if there is nothing missing, in which case just assign tasks.<br><br />
<br />
===Workshop Schedule===<br />
<br />
Luc: PLEASE MAKE A PROPOSITION HERE, with clear question<br><br />
QUESTION:<br />
*ANSWER<br />
<br />
==Point 3: UC coordination/space/agreement==<br />
(WHO, 2min)<br><br />
<br />
To discuss with Benoît and Juliette, clarify needs/hopes and space... <br><br />
<br />
QUESTION:<br />
*ANSWER<br />
<br />
Luc: WHAT IS THE PURPOSE of this discussion? Do we have needs/hopes and what are they?<br><br />
<br />
==Point 4: Future Event Proposals/Scheduling==<br />
(Rachel, 10min)<br><br />
<br />
Already planned:<br><br />
<br />
* OH191 February 28 - [[20180228_OH191 | EPFL iGEM team presentation]] - Luc is in charge.<br />
<br />
QUESTION: Luc requests 100.- minigrant for apéro<br />
*Yes/No<br />
<br />
The following events need to be planned.<br><br />
<br />
QUESTION: Who is taking responsibility?<br><br />
*NAME FOR EACH<br />
<br />
:<li>'''2018.05.02''' Wednesday 19:30-22:00: [[20190502_OH200 | 200 weeks of Hackuarium]] - Should we celebrate? Let us know how!<br />
QUESTION: Who is in charge? Program, advertisement, apéro, etc.<br><br />
*NAME<br />
<br />
:<li>'''2018.03.07''' Wednesday 19:30-22:00: [[20180307_OH192 | General Assembly]] - Annual meeting of the members (all visitors welcome!)<br />
QUESTION: Who is in charge? Program, advertisement, apéro, etc.<br><br />
*NAME<br />
<br />
Luc: I can draft agenda, send an email to all members tomorrow (January 19th), and collect content with deadline for agenda points on 26th January. We then validate all at the next [[20180208_Board_Meeting | board meeting]] so I can send an invitation to all members on that day.<br />
<br />
* Phagetherapy and Lyme disease, public presentations (a Weds in March?)<br />
QUESTION: Who is in charge? Program, advertisement, apéro, etc.<br><br />
*NAME<br />
<br />
Luc: Just pick a date and build a program -> share by email (date, list of speakers, budget) asap<br />
<br />
* Hackuarium Wiki Hackathon (a Saturday soon?)<br />
QUESTION: Who is in charge? Program, advertisement, apéro, etc.<br><br />
*NAME<br />
<br />
Luc: Just pick a date and build a program<br />
<br />
* Hackuarium Wikipedia Hackathon (two days, Tuesday eve at EPFL with Octanis, Weds eve at Hackuarium. if yes, when?)<br />
QUESTION: Who is in charge? Program, advertisement, apéro, etc.<br><br />
*NAME<br />
<br />
Luc: What's the difference with the above?<br />
<br />
* Hackuarium Lab Cleanup (a Sunday soon?)<br />
QUESTION: Who is in charge? Program, advertisement, apéro, etc.<br><br />
*NAME<br />
<br />
Luc: Just pick a date and advertise!<br />
<br />
* Leman Make, 29-30sept<br />
QUESTION: Who wants to take the lead on organising Hackuarium participation?<br><br />
*NAME<br />
<br />
<br />
==Point 5: Next Meetings==<br />
The next board meetings are scheduled for 8feb, and 29mar. <br><br />
<br />
QUESTION: Ok if we do a Doodle for one Tuesday/Thursday each month (in April-May-June-July)?<br><br />
<br />
Luc: can make the propostion<br><br />
RA: agrees on the Doodle<br><br />
<br />
<br />
==Point 6: BeerDeCoded 2018==<br />
(Gianpaolo, 1min)<br />
<br />
* This year we will work on a protocol to generate data in 24h instead of months.<br />
* The major requirement is the access to a 1000$ USB DNA sequencer (Oxford Nanopore).<br />
* Two backers contributed 500 CHF each for BeerDeCoded.<br />
<br />
QUESTION: Is the board contrary to acquire the sequencer? <br />
*Yes/No<br />
<br />
==Point 7: ==<br />
<br />
Anything else?<br><br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=9255Pushing the P12017-06-16T08:48:28Z<p>Randogp: /* Microbes */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
Here is the basic timeline we will try to keep in order to make the project move on. It is obviously a "best case scenario" approximation.<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
The schedule could not be held due to renovations in the building<br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html<br />
to find the official Swiss classification.<br />
<br />
The second step is to borrow the microbe from someone that already used it before. All BSL1 manipulations are announced to the government that makes the database freely accessible here: http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx go and find a researcher. Talking with someone that has experience on that specific microbe will be useful also to get some tips and tricks on growing conditions, etc..<br />
<br />
If nobody worked with the microbe before. It is possible to purchase a culture from the Culture Collection of Switzerland (CH): https://www.ccos.ch/<br />
<br />
Additional repositories:<br />
*Algae and protozoa (UK): https://www.ccap.ac.uk/<br />
*Eukariotic cells and microbes (US):ATCC https://www.lgcstandards-atcc.org/?geo_country=ch Note: the ATCC BSL classification is American. It may differ from the Swiss one. Check the Swiss one before!<br />
*Czech Collection of Microorganisms (CCM) http://www.sci.muni.cz/ccm/index.html<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Backup of the first exploration document : http://wiki.hackuarium.ch/w/Talk:Pushing_the_P1#Old_Hackpad_Page (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
*6. Questions? There is a service in the DIYBio community for [http://ask.diybio.org/ Ask a Biosafety Professional]<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20170607_Board_Meeting&diff=916220170607 Board Meeting2017-06-07T19:15:08Z<p>Randogp: /* Lab Visitor Tally List */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, June 7th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité, 3rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chairpeople==<br />
*Vanessa & Rachel<br />
<br />
==Present==<br />
<br />
*Vanessa<br />
*Anne-Laure<br />
*Rachel<br />
*Gianpaolo<br />
*Gustavo<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Yann Heurtaux<br />
*Yann Pierson<br />
*Daniel<br />
*Sam<br />
*Anna<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Vanessa, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
Will be asked at next board meeting<br />
<br />
==Point 2: Moving into the New space==<br />
(Vanessa, 10 minutes)<br />
<br />
===Practical Questions===<br />
<br />
Luc: is the date confirmed? 24-25 June?<br><br />
Rachel: asked Christophe for confirmation<br><br />
Luc: when do we get this confirmation?<br><br />
<br />
Yes, the moving will be done on June 24th<br><br />
<br />
===Move: Action plan===<br />
<br />
*Wednesday 14 June: 19h-21h evening Design of the Space: planification, attribution of tasks<br><br />
<br />
*Week 19-23 June: all day packing and moving with Gustavo<br><br />
<br />
*Saturday 24 June: 10h-19h with tea time cake party (bring your own lunch)<br><br />
<br />
'''Moving team'''<br />
<br />
Gustavo takes the lead.<br />
<br />
How did it work for the move down to the 1st floor? <br><br />
<br />
People packed their own stuff, then the furniture and the instruments were moved, then the <br />
<br />
Who will be 'lead' member, to contact everyone and make sure we have enough people? <br><br />
<br><br />
From EGA sign up list, these people are available for moving on Saturday 24th<br />
<br />
Gianpaolo, Anne-Laure, Océane, Olivier Emery, Rachel and Vanessa, said they could help during the moving weekend (24-25th June? - RA asked Christophe for confirmation)<br><br />
<br />
*New stuff: Racks? Sofa? <br />
<br />
Yes for storage<br><br />
But we do not get any new furniture before we move (and test the space for at least 2 months)<br><br />
<br />
===Inauguration===<br />
<br />
*Day-long UC event 27 June. Who is signed up?<br> <br />
*Tidy up, participative hacks and show off. (visitors!) <br><br />
<br />
*Hackuarium special event seems like a good idea??<br />
<br />
Vanessa: Je suggere parler avec l'organisation, dans le programme [https://inartis.wufoo.eu/forms/snbf06g0mt45j2/] il n'y a pas des reference à Biohacking / DIYBio / maker / opensource.<br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Vanessa, 15 min)<br><br />
<br />
===Sum up votation from EGA===<br />
<br />
===UC Interactions===<br />
<br />
'''Upcoming Meeting between Benoit and Luc'''<br />
<br />
*items for discussion<br />
<br />
- What value do we bring?<br />
- <br />
- example of people interested in renting space, running<br />
- visibility (press, visitors, etc..)<br />
<br />
*outcome(s) desired<br />
<br />
===Working groups===<br />
Decide on precise timeline and responsibilities NOW!<br><br />
<br />
15 September - 15 September: Campaign<br />
<br />
Then give tasks to working groups.<br />
<br />
* from EGA sign up list:<br />
<br />
Campaign working group: Rachel, Ana, Adrian, Olivier<br><br />
<br />
Communication: Joel, Gianpaolo, Vanessa, Luc, Adrian <br><br />
<br />
Perks: need to contact people and ask for something concrete (workshop, object with timing and budget).<br />
<br />
List of tasks:<br />
<br />
*Storytellers (STELL)<br />
*Communication (COMMS)<br />
*Reward designers (RWD)<br />
*Cash-Flow Team (CASH) <br />
*Logistics (LOG)<br />
*More...?<br />
<br />
===Notes on Workshopology===<br />
<br />
*Campaign perks: each "Workshop Leader" is responsible of the team planning, budget (costs and pricing), materials, participants.<br />
*Needs to communicate with coordination team before mid-July.<br />
*So far, response from the future "workshopology board":<br />
**Oceane Patiny, Luc Patiny, Olivier Emery, Gustavo Santamaria, Rachel Aronoff, Roger Erismann, Vanessa Lorenzo, Sam Sulaimanov.<br />
**Needs to be broken down by topic.<br />
**Best would be to have 2 workshops a month for the 6 months of the winter (November-April) -> 12 dates (each workshop can happen twice -> 6 workshops.<br />
<br />
==Point 4: Other Business==<br />
<br />
===Biofabbing Convergence: Update ===<br />
(Vanessa, 2 min)<br />
*Big thank you and... future "business"???: <br><br />
**Khardana School, Nepal. Contact and funding.<br><br />
**MIT event, Boston. Contact and funding.<br><br />
**[http://forum.hackteria.org/t/diy-bio-fair-play/131/14 | DIY Fair-Play important notes here], EU, funding and more <br><br />
**Hackuarium lab microfuge<br />
<br />
<br><br />
<br />
===Steiner School visit===<br />
(Rachel, 1 min)<br />
*Big thank yous to Vanessa, Gustavo, Dan, and Marc W for the successful visit 22 May!<br />
*Wiki with info: http://wiki.hackuarium.ch/w/Foldscope#Steiner_School_Student_visit<br />
<br />
<br><br />
<br />
===Mozilla Mini-Science Grant===<br />
(Rachel 1 min)<br />
*Application in for project: Personally Tracking Levels of DNA Damage for Citizen Science<br><br />
*Budget especially for making and testing the fluorescence Foldscopes and getting ready for Cheek Cell citizen science data.<br><br />
*Workshops for November 2017, and February 2018, with big week-long workshop for March 2018, in plan.<br><br />
*Announcement expected end of the month (unlikely to be awarded, but positive feedback already about project)...<br />
<br />
<br><br />
<br />
===Lab Visitor Tally List===<br />
(Rachel 1 min)<br />
* Spreadsheet for keeping track of lab visitors, esp large touring groups who want 'open lab' spiel...<br />
This idea can also help document our 'added value' for UC. <br><br />
Please 'bookmark' this spreadsheet in your devices and fill it in when people come to lab! https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing<br><br />
<br><br />
<br />
===Montreux Clean Beach Project 3 ===<br />
(Rachel 1 min)<br />
*water sampling for microbial analyses beginning!<br />
Monday 'data collection,' sampling, and plating, Tuesday/Weds scoring 12 June-3 August <br> <br />
Help is welcome!<br><br />
<br><br />
<br />
===Radio Griff===<br />
* reportage pour GRRIF<br />
Guillaume Truffer will come at 14:00 on Friday 9 june, if someone wants to talk about their project for the radio!<br />
(podcast, too...)<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Projects:LivingLab_Design&diff=9046Projects:LivingLab Design2017-06-02T12:17:24Z<p>Randogp: /* REPORT of first Livinglab workshop */</p>
<hr />
<div>LivingLab design is a series of workshop designed and facilitated by [[user:Katalin|Katalin Hausel]]. <br />
<br />
<br />
== Context ==<br />
<br />
<br />
The UniverCite co-working space is launched, given 16 months to build a live community and network of the business/scientific/artistic community within the Lausanne-Renens area. It is hoped to prove itself to be useful either for the commune, and/or businesses, and/or individual members to become economically stable after this initial period. Before addressing specific design issues, let's look at the big picture: let's try to design the near-future of the lab by asking questions, sharing the background of the different stakeholders to support this project. How can we build a common vision for the space? How can we set shared achievable goals that integrate everyone's own? How can the lab itself benefit from the diverse community it hopes to attract? <br />
<br />
<br />
== Goals ==<br />
<br />
<br />
To understand the different interests and motivations behind the project; to<br />
have a shared idea of the future and how to start making it happen; to<br />
forge teams/collaborations and to make <br />
plans to tackle foreseeable challenges.<br />
<br />
== Method ==<br />
<br />
- listen always to what is emerging, don't see the world just through an Excel spreadsheet<br />
<br />
- build raw and fast prototypes, collect feedback, fine-tune and iterate till needed<br />
<br />
- trust the collective and individual attitudes and skill<br />
<br />
== Workshop Specifics ==<br />
<br />
<br />
'''To ask questions:'''<br />
<br />
VISION: what is the vision of the space in a year's time for each workshop participant <br />
<br />
EXPECTATIONS: we need to understand the expectations of stakeholders, including communities which will inhabit the space<br />
<br />
CHALLENGES: what are the foreseeable challenges for the project <br />
<br />
VALUES: what are the core values the space needs to reflect <br />
MOTIVATION: what are the personal motivations for each person to work on the project <br />
<br />
GOALS: what are the goals for each stakeholder <br />
<br />
SUCCESS CRITERIA: for each person - 3 months, 6 months, 9 months, 12 months <br />
<br />
<br />
'''To design the near future:''' <br />
<br />
VISION: let's create a shared vision <br />
<br />
VALUES: what are our shared values <br />
<br />
TIMEFRAME: create a shared timeline <br />
<br />
GOALS: identify shared goals and place them within the timeframe <br />
<br />
CHALLENGES: how to address specific challenges <br />
<br />
SUCCESS CRITERIA: within the timeframe <br />
<br />
COLLABORATION: teams; how to utilise the expertise of the emerging community of the space <br />
<br />
TAKING CARE OF EACH OTHER: follow-up; a platform to trace progress and voice concerns; set a regular weekly meeting time for optional face-to-face exchange <br />
<br />
<br />
'''References'''<br />
<br />
http://www.artisopensource.net/projects/near-future-design.html <br />
<br />
http://www.shareable.net/ <br />
<br />
http://www.methodkit.com/ <br />
<br />
https://docs.google.com/document/d/1wyIje9lqc1oFF7viViN6Tpls3p0J50GmTbuD47_m-XY/edit<br />
<br />
== Workshop OUTLINE ==<br />
<br />
1. '''Check-in (circle)'''<br />
<br />
''Who are you?<br />
What brings you here?''<br />
<br />
2. '''COLLECTING personal inquiries''' - tools: post-it notes, pens, board to stick them on<br />
<br />
''ask to write and read them aloud one by one: vision; values, timeframe, etc<br />
identify overlaps and contradictions'' <br />
<br />
''break''<br />
<br />
3. '''FINDING common future(s)''' - tools: another board (white), post-it notes from previous session, markers<br />
<br />
* '''Shared aspirations:''' VISION, VALUES, GOALS<br />
<br />
''each person tell a scenario for the future of the lab that incorporates as many visions, goals, values on the board as possible (based on previous "Wow!" moments)<br />
work on a shared narrative; try to tweak the central narrative so that everyone feels that their most important personal goals etc are included''<br />
<br />
* '''Shared responsibilities:''' TIMELINE, CHALLENGES, SUCCESS CRITERIA<br />
<br />
''in the shared narrative for the space, how deliverables will be delivered and problems solved (expand the narrative)''<br />
<br />
4. '''WORKING together to make it happen''':<br />
<br />
''-personal expertise, how can you contribute, form teams<br />
-how to bring people on: the emerging community in the lab, finding specialists<br />
-follow-up - how to communicate successes and problems, how to keep the group working together, how to meet face-to-face<br />
''<br />
<br />
5. '''Check-out (circle)'''<br />
''<br />
What do you take away from the workshop?''<br />
<br />
<br />
<br />
== REPORT of first Livinglab workshop ==<br />
<br />
'''Workshop held on Sunday 28 October'''<br />
<br />
Participants: Luc, Yann, Carmelo, Marc, Gianpaolo, Sachiko, Urs, Katalin Hausel<br><br />
<br />
The idea was proposed by Katalin, to collect individual responses and then try to imagine the (near) future of the lab, in which most responses are accommodated, in the form of a narrative. Everyone, first person by person for each concept, later more intuitively associating and moving freely, articulated their response to the following questions:<br><br />
<br />
*VISION: what is the vision of the space in a year's time for each workshop participant?<br />
*EXPECTATIONS: (this was thrown in together with goals and values)<br />
*CHALLENGES: what are the foreseeable challenges for the project?<br />
*VALUES: what are the core values the space needs to reflect?<br />
*MOTIVATION: what are the personal motivations for each person to work on the project?<br />
*GOALS: what are the goals for each stakeholder?<br />
*SUCCESS CRITERIA: for each person - 3 months, 6 months, 9 months, 12 months<br />
<br />
There were a few rules:<br><br />
<br />
*Responses should be based on personal positive experiences, which can serve as models for the Lab.<br />
*Recall an event when you felt "wow, this is why I want to collaborate/to work in a co-working place/ to meet new people; etc", rather than saying “I hate {x}, and so my goal is to create non{x}”. <br />
*No computers. The point is to pay attention to each other, and feel being listened to.<br />
<br />
The idea was to move from first individual to shared aspirations (visions, goals, motivations), from there to measurable goals with timeframe, and from there to small tasks with deadlines that people can form teams around. Although we did not get to the “shared” part, the session already proved the approach useful. We walked away with a fairly detailed understanding of each other’s motivations and goals, and small deliverable tasks that already move things towards reaching them.<br><br />
<br />
''CONTRIBUTIONS:''<br><br />
<br />
Vision<br><br />
<br />
*nice & humble people<br />
*a place to MAKE<br />
*explore ways of different knowledge distribution<br />
*create new business model for biotech<br />
*sharing, responsible community (culture!)<br />
*a hub for innovation<br />
*a place for diverse competences<br />
*a perfect place for freelance work<br />
*nurture independence and freedom in work<br />
*mesh meeting<br />
*connective vision<br />
*“out” (beyond?) institution // Switzerland<br />
*local community<br />
<br />
Values<br><br />
<br />
*self-governance<br />
*openness<br />
*spontaneity<br />
*democracy<br />
*independence<br />
*sharing<br />
*growth<br />
*synergy<br />
*open source, open everything<br />
*alive<br />
*sustainability<br />
*responsibility (be excellent to each other)<br />
*transparency<br />
*trust<br />
*safety<br />
*ethic code diy bio/ no harmful diy/ no guns<br />
<br />
Goals/Expectations:<br><br />
<br />
*complementary with existing structures (of working)<br />
*ideas/values pollination<br />
*learn from other similar projects<br />
*educating/training<br />
*public events<br />
*create a hub/node<br />
*answer actual needs for freelancers (wifi; coffee; events; networking)<br />
*mapping of competence needs<br />
*recruit<br />
*attract everybody, not nerds only<br />
*gender balance<br />
*new business model// ecosystem <br />
*gain independence (weaning) <br />
*library /digital+analog/<br />
*kitchen<br />
*gallery<br />
*produce rich documentation<br />
*quality tools<br />
<br />
Challenges:<br><br />
<br />
*burn-out<br />
*existing science model, which is exclusive, protective <br />
*to communicate culture low cost / low-tech<br />
*to articulate values<br />
*to build awareness<br />
*to nurture distributed projects<br />
*to become visible<br />
*to set clear guidelines<br />
*make a manual<br />
*identify tools<br />
<br />
Success criteria (subjective and/or objective):<br><br />
<br />
*self-sustainable<br />
*carefully picked dependencies<br />
*in 15 months, we are doing better<br />
*set higher standards/ better coworking culture in the area<br />
*30 stories in 3 months<br />
*gender balance<br />
*very independent members<br />
*plan “B” risk management<br />
*a community strong enough to be able to survive moving<br />
*50 users in 3 months<br />
*30 projects in 6 months<br />
*co-designed space in 6 months<br />
<br />
NEXT MEETING: Oct. 10<br><br />
<br />
tasks/people:<br><br />
<br />
*draft preliminary report / Luc<br />
*preliminary inventory / Yann P. +Sachiko<br />
*draft business model / Carmelo<br />
*dirty lab wish list / Marc<br />
*list of available (digital) tools / Yann H.<br />
*description of ideal entrepreneur environment (link on discussion http://wiki.hackuarium.ch/w/Talk:Projects:LivingLab_Design#Gianpaolo.2C_Oct_10_2014.2C_Description_of_the_Ideal_Entrepreneur_Environment) / Gianpaolo<br />
*report of this session / Katalin<br />
*interviews and a survey on website / Katalin<br />
<br />
== REPORT of second LivingLab workshop ==<br />
<br />
'''Near Future Design Part 2 - workshop held on 10.10.2014'''<br />
<br />
This session with small reports on the following:<br><br />
<br />
*draft preliminary report / Luc<br />
*preliminary inventory / Sachiko<br />
*draft business model / Carmelo<br />
*dirty lab wish list / Marc<br />
*list of available (digital) tools / Yann H.<br />
*description of ideal entrepreneur environment / Gianpaolo<br />
*report of the first session / Katalin<br />
*interviews and a survey on website / Katalin<br />
<br />
The conversation that span out of the presentations threw out the following ideas:<br><br />
<br />
*create a skills database//marketplace (through website)<br />
*wiki sprint to create website<br />
*the dirty lab can become a revenue source (needs a budget)<br />
*safety rules for lab is necessary before it opens<br />
*separation of the space to seal noisy/smelly/toxic tools<br />
*we need a co-napping space<br />
*we need a shower<br />
*we need a kitchen<br />
*we need centralised image depository<br />
*social media presence for Univercity<br />
*RECRUITMENT (forge partnerships)<br />
*network with outside (hackers and non-hackers)<br />
*events and shared meals<br />
<br />
In the second part of the session we placed the various goals, expectations and problems to solve into a timeframe:<br><br />
<br />
within 1 week:<br><br />
*produce rich documentation / YH, CB<br />
*answer actual needs for freelancers: wifi, coffee, networking / YH, CB<br />
<br />
within 3 months (by January):<br><br />
*co-governance / YH, CB, LH<br />
*a perfect workplace for freelance entrepreneur work / YH, GR<br />
*quality tools / MY<br />
*a place to MAKE / MY<br />
*transparency / CB, YH, LH<br />
*kitchen / CB, KH<br />
*recruite / LH, PR<br />
*more nice people / LH<br />
*PARTY / everyone<br />
*plan "b"; risk management / CB, YH<br />
*nurture independence, freedom in work / unassigned<br />
*safety / Dirty Lab: MY, KH; Chem/Bio: LH<br />
*write description of complementarity with existing structures (of bio research) / LH<br />
*education and training / LH<br />
*ideas/values pollination / LH<br />
*library digital+analog / unassigned<br />
*map competence needs / SH<br />
*trust / YH, KH<br />
*users 50 people by January / PR<br />
*MANUAL / YH<br />
*clear guidelines + organisation /YH, CB<br />
*articulate values / KH<br />
*communicate culture (low-cost/low-tech) / unassigned<br />
*become visible / PR, KH, LH, CB<br />
*build awareness / PR, KH, LH, CB<br />
*openness / YH, KH<br />
*responsibility 'be excellent to each other" / KH, YH<br />
<br />
6 months (by April):<br><br />
<br />
*attract everybody, not only nerds<br />
*carefully picked dependencies<br />
*democracy<br />
*gallery<br />
*30 projects<br />
*co-design<br />
*humble people<br />
*nurture distributed projects <br />
*synergy<br />
*gender balance<br />
<br />
9 months (by July):<br />
<br />
*hub/node events<br />
*creating new business model for biotech<br />
*new business model/ecosystem<br />
<br />
12 months (by October):<br />
<br />
*sustainable<br />
*a place for diverse competences<br />
*gain independence (weaning)<br />
*growth<br />
*"doing better"</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Talk:Projects:LivingLab_Design&diff=9045Talk:Projects:LivingLab Design2017-06-02T12:16:38Z<p>Randogp: /* Gianpaolo, Oct 10 2014, Description of the Ideal Entrepreneur Environment */ new section</p>
<hr />
<div>== Gianpaolo, Oct 10 2014, Description of the Ideal Entrepreneur Environment ==<br />
<br />
(Originally written on Hackpad in 2014, pasted here on June 2nd 2017 after Hackpad shut down)<br />
<br />
Cowocondo is the coworking condo: the ideal entrepreneur environment having the mission to:<br />
*generate startup models (conception),<br />
*make them ready to live alone (weaning)<br />
*generate new cowocondos (life cycle).<br />
<br />
<br />
Cowocondo accomplish this mission executing 5 key steps, one step at time.<br />
<br />
1. Merges two groups of smart people: Innovators and Facilitators.<br />
2. Makes co-work and community extremely valuable.<br />
3. Introduces Innovators to potential partners & investors.<br />
4. Supports key startup steps with seed money injection.<br />
5. Becomes home of Innovators and Facilitators.<br />
<br />
*Step 1 - To merge two groups of smart people: Innovators and Facilitators.<br />
Innovators: people apt to conceive new product/technology. They want to make the future a better world by developing new tools. They will share future % with the co-working community, facilitating future cycles of innovation.<br />
Facilitators: people apt to support innovation by mastering current tools. They help the Innovators to craft a prototype. In addition, they work for externals and share % revenue with the co-working community, making the current innovation process more sustainable.<br />
Goal: attracting 12 Innovators in 6 months.<br />
Not impossible: we are in 6, we could recruit 2 each.<br />
Goal: attracting 18 Facilitators.<br />
This requires more thinking. We need masters in culture, conversation, chemistry, biology, agronomy, design, prototyping, App dev, storytelling, accounting, marketing, user testing, sales.<br />
Note: Innovator/Facilitator is not a label, and most importantly, it is not a rank. It is a function that might be attributed also to the same person.<br />
<br />
<br />
*Step 2 - To make co-work and community extremely valuable.<br />
Goal: To favor internal collaboration, disfavor internal competition.<br />
Goal: To make one's success the success of all the co-workers.<br />
Strategy: To protect and favor uninterrupted creative work.<br />
Manage all external money flow (bills, invoices).<br />
Manage in/out parcels/trash. (some reagents are shipped frozen)<br />
Make easy to satisfy body requests (hungry, sleep)<br />
Strategy: To favor internal money transfer (bitcoin?)<br />
Implement leverage between Facilitators & Innovator (see example of trading platforms)<br />
<br />
<br />
*Step 3 - To introduce Innovators to potential partners & investors.<br />
Example/Inspiration: YC weekly dinners. https://www.ycombinator.com/atyc/#dinners<br />
Goal: 6 hr / month of external mindshare.<br />
Goal: Demo day at the term.<br />
<br />
* Step 4 - To support key startup steps with seed money injection (not default).<br />
Goal: to find people investing in Cowocondo first!<br />
Strategy: investing chunks of 15,000 CHF for 1% ownership<br />
<br />
* Step 5 - To become home of Innovators and Facilitators.<br />
Inspiration: Unmonastery. http://unmonastery.org/<br />
Inspiration: Paul Graham "I'd advise most startups to avoid corporate space at first and just rent an apartment" http://www.paulgraham.com/start.html "You want to live at the office in a startup, so why not have a place designed to be lived in as your office? [...] The key to productivity is for people to come back to work after dinner. Those hours after the phone stops ringing are by far the best for getting work done. Great things happen when a group of employees go out to dinner together, talk over ideas, and then come back to their offices to implement them."<br />
Inspiration: Yesteday's Nobel prize. In 2005, two unemployed guys craft a microscope in their apartment. Today (9 years later) they win the Nobel prize. Their miscroscope (PALM) break the Abbey limit paving the way to super-resolution optical microscopy. If they could make it at home, what could we do in a coworking condo?<br />
Long-term Goal: Cowocondo = co-working + co-housing = co-living space<br />
Provide a 7/7 24/24 source of inspiration.<br />
Provide a 7/7 24/24 peace of mind.<br />
Provide a 7//7 24/24 working opportunities.</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=9043Pushing the P12017-06-02T06:43:11Z<p>Randogp: /* Microbes */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
Here is the basic timeline we will try to keep in order to make the project move on. It is obviously a "best case scenario" approximation.<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
The schedule could not be held due to renovations in the building<br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html<br />
to find the official Swiss classification.<br />
<br />
The second step is to borrow the microbe from someone that already used it before. All BSL1 manipulations are announced to the government that makes the database freely accessible here: http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx go and find a researcher. Talking with someone that has experience on that specific microbe will be useful also to get some tips and tricks on growing conditions, etc..<br />
<br />
If nobody worked with the microbe before. It is possible to purchase a culture from the Culture Collection of Switzerland (CH): https://www.ccos.ch/<br />
<br />
Additional repositories:<br />
*Algae and protozoa (UK): https://www.ccap.ac.uk/<br />
*Eukariotic cells and microbes (US):ATCC https://www.lgcstandards-atcc.org/?geo_country=ch Note: the ATCC BSL classification is American. It may differ from the Swiss one. Check the Swiss one before!<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Backup of the first exploration document : http://wiki.hackuarium.ch/w/Talk:Pushing_the_P1#Old_Hackpad_Page (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
*6. Questions? There is a service in the DIYBio community for [http://ask.diybio.org/ Ask a Biosafety Professional]<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=9036Pushing the P12017-06-01T07:49:57Z<p>Randogp: /* Legal */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
Here is the basic timeline we will try to keep in order to make the project move on. It is obviously a "best case scenario" approximation.<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
The schedule could not be held due to renovations in the building<br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html<br />
to find the official Swiss classification.<br />
<br />
The second step is to borrow the microbe from someone that already used it before. All BSL1 manipulations are announced to the government that makes the database freely accessible here: http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx go and find a researcher. Talking with someone that has experience on that specific microbe will be useful also to get some tips and tricks on growing conditions, etc..<br />
<br />
If nobody worked with the microbe before. It is possible to purchase a culture.<br />
One well recognised repository is ATCC: https://www.lgcstandards-atcc.org/?geo_country=ch Note: the ATCC BSL classification is American. It may differ from the Swiss one. Check the Swiss one before!<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Backup of the first exploration document : http://wiki.hackuarium.ch/w/Talk:Pushing_the_P1#Old_Hackpad_Page (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
*6. Questions? There is a service in the DIYBio community for [http://ask.diybio.org/ Ask a Biosafety Professional]<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Talk:Pushing_the_P1&diff=9035Talk:Pushing the P12017-06-01T07:48:20Z<p>Randogp: /* Old Hackpad Page */ new section</p>
<hr />
<div>Keep in mind that for storing competent bacteria, skim milk is superior to glycerol. See the numbers of recoveries after hurricane Katrina here http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2551311/<br />
<br />
We can start without -80 °C. In old times, the postdoc would grow bacteria overnight, make them competent in the morning, transform them in the evening.<br />
<br />
== Old Hackpad Page ==<br />
<br />
Wanna go P1 ?<br />
Introduction:<br />
Ultimately one of our fixed goal is to be able to work in a P1 environment. This would open the doors to a vast range of experimental possibilities and a important value added to a space like ours. But like Stanley Martin Lieber said: "With great power comes great responsibilities". This quote in my opinion reflects with exactitude what working with a P1 is. On one hand greater experimentation freedom could come out of a space like this, most probably wider impact results, the scientific thrill of getting access to advanced biotechnological tools & a bigger ponderation among the scientific community be it corporative & academic. On the other hand, the risks of environmental bacteriological releases, a stricter working condition, the need of righteous, self disciplined collaborators and neatly defined boundaries and follow up procedures are a counterpart that should not be overlooked. I strongly believe achieving such performances is in our reach and working towards it is, per se, a goal worth investing energy and capitals in.<br />
<br />
A word on micro-organisms safety denomination:<br />
You can find a decent summary on wiki: http://en.wikipedia.org/wiki/Biosafety_level<br />
<br />
Requirements: Legal & Ethics:<br />
Defining boundaries is a first step towards such achievement. What better guidelines is there than the Federal Act on Non-Human Gene Technology (LGG), The Ordinance on Handling Organisms in Contained Systems (OUC) and the WHO's manuel de sécurité en laboratoire (MSBL). Note that any legal act is studied in french since it has no legal power in English. Here I try to answer several questions using those two base texts and my day-to-day life in a P1 lab as a chemical biology PhD student but first a small summary on the main ideas and intentions contained in both texts:<br />
<br />
LGG: The LGG has as main goal to protect and to serve welfare of all living beings against abuses in genetical engineering (GE). The aim of LGG is to protect the health and safety, conserve the biological diversity & to ensure respect to all living beings. It also precludes hazards and harms caused by GE.<br />
MSBL: The MSBL is focused on good laboratory practices, security means to avoid hazards and harms & takes on the topic of international regimentation on GE and GMO transport. The MSBL gives extensive guidelines on the the good microbiology techniques (GMT). This document is the milestone in the ethics and GMO management and most of its items go far beyond what is the scope of our goal.<br />
The take home message: Safety, standardized procedures & professionalism! <br />
The following document is a good summary of what is asked to get a P1 and the general legislative implications<br />
http://www.cusstr.ch/repository/133.pdf<br />
<br />
What requirements / equipment is needed for a lab to be a P1 lab ? <br />
What formation is needed for the coworkers to work in a P1 lab ?<br />
What is the range of activities allowed in a P1 lab ?<br />
How should the waste be treated ?<br />
How should the incoming and outgoing accesses to the lab be limited & controlled ?<br />
What personal protective equipment is required in a P1 lab ?<br />
Working zone behavior: what is the good behavior and how should the work bench be treated ? <br />
To whom should we announce our will to work with GMOs and to whom should we notify our activities ?<br />
other obligations<br />
<br />
a: What requirements / equipment is needed for a lab to be a P1 lab ? <br />
from MSBL: <br />
People should have a standardized, basic microbiology formation and strictly follow good laboratory practice. Material wise the safety is limited and work can be done in the open. Any apparatus can be operated in the same room no separated room for certain operations is needed. <br />
ref: page 1 to 4<br />
<br />
b: What formation is needed for the coworkers to work in a P1 lab ?<br />
from MSBL:<br />
GMT are a must! They increase the safety of all occupants and the operator. Every lab has to be equipped with manuals describing the operation of each tool, microbiology operation and safety measures. All security means necessary have to be taken into account and organized such that every member working in the P1 area knows how to react and operate the safety means and procedures.<br />
ref: page 9 - 10<br />
Continuous "on the fly " formation and safety sensitization of the collaborators has to be a day-to-day task. <br />
ref: page 18<br />
<br />
c: What is the range of activities allowed in a P1 lab ?<br />
from MSBL:<br />
If a lab is doing biological analysis, Biological Safety 2 (BS2) is to be observed since the samples are not always known. (Item needs to be studied in greater detail / it is not in the scope of our goal) <br />
ref: page 9<br />
from experience:<br />
Organisms modification can be made, iGEM style, through building blocks or standardized procedures.<br />
<br />
d: How should the waste be treated ?<br />
from MSBL:<br />
Contaminated fluids and solids have to be decontaminated by chemical or physical means. <br />
Different compartments have to exist in order to store separately non contaminated wastes, cutting or sharp objects (syringes), contaminated solids & contaminated liquids.<br />
ref: page 19-21<br />
from experience:<br />
Liquids are neutralized with 20% bleach. Solids are autoclaved in a double layer of plastic bags before being thrown away. (Question: do we need a special waste service ? ) . Usually needles are not used in microbiology and molecular biology. A document describing the process has to be available.<br />
from cusstr:<br />
All wastes contaminated by GMO have to be inactivated (autoclave) before being eliminated by a normal waste treatment.<br />
<br />
e: How should the incoming and outgoing accesses to the lab be limited & controlled ?<br />
from MSBL:<br />
No person foreign to the working area is allowed to penetrate the working zone. Lab doors have to be closed and have a log and locking system. No kids and pets are allowed in the working zone. <br />
ref page 10-11<br />
<br />
f: What personal protective equipment is required in a P1 lab / What are the security requirements?<br />
from MSBL: (non exhaustive list - removed common sense articles...)<br />
The standard "chemistry blouse" is to be worn. <br />
Appropriate gloves have to be worn when manipulating biological material and hands must be disinfected afterwards. <br />
No food is allowed in the lab.<br />
No open shoes are allowed in the lab.<br />
ref: page 10 to 15<br />
from OUC:<br />
Art 12. safety measures:<br />
The general safety measures listed in Annex 4 and the special safety measures required according to the type and class of activity must be taken, and an operational safety concept must be devised. The safety measures taken must take account of the risk determined in the individual case and the state of the art of safety technology.<br />
<br />
g: Working zone behavior: what is the good behavior and how should the work bench be treated ? <br />
from MSBL: <br />
The bench has to be clean of work unrelated objects and disinfected at the end of the day. Every piece of material has to be decontaminated before being thrown away or cleaned if reused. <br />
from experience:<br />
Cluttering is quite common. Anyhow you still have lots of space to work and nothing unrelated is on the benches.<br />
<br />
h: To whom should we announce our will to work with GMOs and to whom should we notify our activities ?<br />
from OUC:<br />
Art 8: Any person who wishes to carry out Class 1 activities with genetically modified organisms must notify this globally, at the latest when beginning the activities.<br />
<br />
Art. 11: Submission to the authorities<br />
Notifications and authorisation applications must be submitted to the Federal Coordination Centre for Biotechnology. (BAFU)<br />
Notifications and authorisation applications must include the information listed in Annex 3. In the information, procedures and methods related in their nature, extent and purpose may be summarised.<br />
The information may be entered directly into the electronic database provided by the Federal Coordination Centre for Biotechnology.<br />
i: other obligations:<br />
from OUC:<br />
<br />
Documents<br />
World Health Organisation (MSBL):<br />
WHO's manuel de sécurité en laboratoire<br />
Loi sur le Genie Genetique (LGG):<br />
Federal Act on Non-Human Gene Technology <br />
Loi fédérale sur la protection de l'environnement (LPE):<br />
http://www.admin.ch/opc/fr/classified-compilation/19830267/index.html<br />
Loi fédérale sur la lutte contre les maladies transmissibles de l'homme (Loi sur les épidémies)<br />
http://www.admin.ch/opc/fr/classified-compilation/19700277/index.html<br />
Loi du 1er juillet 1966 sur les épizooties (LFE)<br />
http://www.admin.ch/opc/fr/classified-compilation/19660145/index.html<br />
Ordonnance sur l’utilisation d’organismes dans l’environnement (ODE)<br />
http://www.admin.ch/opc/fr/classified-compilation/20062651/index.html<br />
Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes (OPTM) <br />
http://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
Ordonnance sur l’utilisation des organismes en milieu confiné (OUC)<br />
http://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
<br />
Autorities <br />
Bureau de biotechnologie de la Confédération (BAFU)<br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr<br />
http://www.efbs.admin.ch/fr/themes/index.html<br />
<br />
Waste treatment<br />
According to the following document and from the EPFL and UNIL directives, elimination of wastes in a P1 environment is pretty straightforward. Both liquid wastes and solid wastes can be autoclaved. Autoclaved liquid wastes can be the poored down the drain, solid wastes have to be transferred to a white and red thick plastic bag and be eliminated according to the procedure 18 01 03 ds on the OMoD website. Usually the town seems to be able to proceed to such wastes evacuation.<br />
<br />
refs:<br />
general : http://www.cusstr.ch/repository/133.pdf<br />
EPFL : http://www.cusstr.ch/repository/175.pdf<br />
UNIL : http://www.unil.ch/files/live//sites/fbm/files/shared/securite/Pro_032_traitement_dechet_01.pdf<br />
OMoD : https://www.veva-online.admin.ch/<br />
<br />
Therefore, we can expect to generate little amount of solid wastes and the only real problem now is to set fixed rules on the usage of the lab as a P1 Lab.<br />
<br />
<br />
What's next then ?<br />
Honestly I think we are not prepared for that yet. Material and organisation wise much needs to be done. <br />
The lab should be more clearly defined in terms of space enclosures, meaning the access to Hackuarium should not directly communicate with the green space and the outside but people should enter FROM the green space and the though the conference room and afterwards if part of the team or coming for a visit (and therefore escorted) be allowed inside.<br />
In terms of instruments, a clear operation booklet is to be put close to it, people should have a formation by a member. Instrument usage should be monitored to make sure they are functional and not getting used in a incorrect way.<br />
In terms of manipulation, none should be allowed without a proper formation. And the documentation should be much more thorough! <br />
The project follow up have to be thorough, nobody should be allowed to do a project without the accordance of the board and without a clear project file explaining what will be done, trough which modifications, using which bio-tool or transformation.<br />
There should be common stocks, like electricians have a rack with components, we biologist should have a freezer with common competent cells, common base plasmids, common stock solutions.<br />
The need of a -80°C freezer.</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20170412_Board_meeting&diff=876220170412 Board meeting2017-04-10T09:35:28Z<p>Randogp: /* Point 3: Varia */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, April 12th, 20.00-22.00pm<br />
<br><br />
Place: UniverCité, 3rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Meeting=<br />
<br />
==Chairpeople==<br />
*Vanessa & Rachel<br />
<br />
==Present==<br />
<br />
*<br />
<br />
==Excused==<br />
<br />
*<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Vanessa, 1 min)<br />
<br />
==Point 0: public minutes==<br />
(Vanessa, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: UC Crowdfunding campaign==<br />
<br />
===Update from the President===<br />
(Vanessa, 5 min)<br />
<br />
Renens to provide a great part of funds (0.8M of 1.2M needed), and Vanessa and Rachel (and Gustavo) had a nice lunch with the syndic, Jean-François Clément.<br />
<br />
Edouard Treccani, who works for Inartis/MC, is the main contact for the Crowdfunding, which has a 'soft launch' planned for the night of the political debate about Innovation in Vaud (11apr), and the actual 'digital' launch sometime in the next month.<br />
<br />
Who else wants to work on this? Who is ready to devote significant time over the next 6 weeks? We need people sharing news, making 'perks' for crowdfunding and probably much more!<br />
<br />
<br />
==Point 2: Community Happiness==<br />
(Shalf, 10 min)<br />
<br />
===Microgrants===<br />
*[[Microgrant Process Review]] shared, i.e. work has started. Shalf will keep improving this page until next board meeting for a structured conversation to happen.<br />
<br />
===Ateliers de Renens===<br />
Next steps proposed and approved:<br />
*Follow-up w/ Christophe re: stolen wood<br />
*Check next UC Community Meeting date & governance. Yann will update the board & community to make sure we have time to properly gather feedback from everyone, and will go with someone else from the Board (depending on meeting's agenda and availabilities).<br />
<br />
===Community Strength===<br />
<br />
How many people are really paying members?<br />
How often do more than the core 5-10 actually come to the lab??<br />
...<br />
<br />
==Point 3: Varia==<br />
===Implement automatic reply===<br />
(Luc, 2 min)<br />
<br />
For the hello@hackuarium email address.<br><br />
We would need it both in French and English. Based on the email from London Biohackerspace:<br><br />
<br />
Thanks for your email and for being interested in what we do!<br />
We will come back to you as soon as possible but in the meantime please have a look at<br />
our website: http://www.hackuarium.ch<br />
our wiki: http://wiki.hackuarium.ch<br />
Hackuarium is a volunteer-led space and we need to use our time wisely.<br />
If you want to talk to us, we meet at 19:30 every Wednesday evenings at<br />
UniverCité, Chemin du Closel 5, 1020 Renens<br />
Cheers!<br />
The Hackuarium team<br />
<br />
Luc would be happy to polish the text and send it to Vanessa for implementation.<br />
<br />
===Update Website===<br />
(Luc, 2 min)<br />
<br />
Sam -> any idea of the number of visitors?<br><br />
Add profile of all members.<br><br />
Update projects description.<br><br />
<br />
<br />
===Update BeerDeCoded===<br />
(Gianpaolo, 1 min)<br />
<br />
We have the DNA raw data of 39 beers. <br><br />
Communication plan<br><br />
<br />
==Point 4: Events==<br />
(YY, XX minutes)<br />
<br />
===Biofabbing_Unconference===<br />
(Vanessa, 15 min)<br />
<br />
[http://citizensciences.net/biofabbing/ Biofabbing conference] will happen on May 10-13.<br><br />
<br />
<br />
==Point 5: Future activities==<br />
(Vanessa, 2 min)<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=8722Pushing the P12017-03-24T12:41:39Z<p>Randogp: /* Providers */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
Here is the basic timeline we will try to keep in order to make the project move on. It is obviously a "best case scenario" approximation.<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
The schedule could not be held due to renovations in the building<br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html<br />
to find the official Swiss classification.<br />
<br />
The second step is to borrow the microbe from someone that already used it before. All BSL1 manipulations are announced to the government that makes the database freely accessible here: http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx go and find a researcher. Talking with someone that has experience on that specific microbe will be useful also to get some tips and tricks on growing conditions, etc..<br />
<br />
If nobody worked with the microbe before. It is possible to purchase a culture.<br />
One well recognised repository is ATCC: https://www.lgcstandards-atcc.org/?geo_country=ch Note: the ATCC BSL classification is American. It may differ from the Swiss one. Check the Swiss one before!<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Hackpad first exploration document : https://hackpad.com/Wanna-go-P1--I8F1GOCYTR9 (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=8721Pushing the P12017-03-24T12:21:24Z<p>Randogp: /* Providers */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
Here is the basic timeline we will try to keep in order to make the project move on. It is obviously a "best case scenario" approximation.<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
The schedule could not be held due to renovations in the building<br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
To check if the microbe you want to work is P1, go to http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Hackpad first exploration document : https://hackpad.com/Wanna-go-P1--I8F1GOCYTR9 (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Logo&diff=8700Logo2017-03-13T08:45:30Z<p>Randogp: </p>
<hr />
<div>Our '''logo''' has been designed by our dear friend and fellow member [http://xdiverres.ch/ Xavier Diverres], in the early days of [[Hackuarium]].<br><br />
Not fancying himself as a graphic designer (he prefers interactions), Xavier made a pretty good job nonetheless! Xavier is also a coworker at [[UniverCité]].<br><br />
(.eps and .zip versions are coming)<br />
[[File:Hackuarium logo15.png]]<br />
==Concept==<br />
We wanted a water-based animal to play with the words Hackuarium / Aquarium, the octopus was chosen because he can grabs many things, the octopus is also an animal with a distributed brain: each tentacle has an independent brain, still the octopus acts as a single organism. This represents well the spirit of our community.<br />
<br />
==Logo History==<br />
We first started investigating the idea of a logo and proposed it on networks at the beginning of the space:<br />
<br />
To look at the history it happend like this:<br />
Shalf said:<br />
http://www.facebook.com/hackuarium/posts/602903189823337<br />
"To make it serious we'll need a logo"<br />
<br />
Yann Pierson thought:<br />
What did the others do? If we want to be "hackers" we'll need to reach out, reach out far and a lot! What animal could be the best: An octopus:<br />
and came up with this research:<br />
https://drive.google.com/file/d/0B7j153lmyn_eLVZfMHMzOW5DTkNMWnpkcEUtWUVSVmtrWXVz/edit<br />
that showed the orientation for the "water based animals" maybe a medusa ?<br />
https://hackpad.com/Identitybrandingcontent-IZxV3yauzbE<br />
Then he designed the big black octopus picture but as the others correctly said "It's way to complicated" =)<br />
<br />
Showed our progress and research on the collaborative work made in the space<br />
http://www.facebook.com/hackuarium/posts/618314418282214<br />
<br />
So Xavier did his magic and came with the final result!<br />
And first presented it to the world =)<br />
http://www.facebook.com/hackuarium/posts/F645853502194972<br />
<br />
Voilaaaaa</div>Randogphttps://wiki.hackuarium.ch/index.php?title=BeerDeCoded&diff=8683BeerDeCoded2017-03-11T18:26:24Z<p>Randogp: /* How did we NOT finance the project? */</p>
<hr />
<div>'''BeerDeCoded''' is a quest to decode the molecular composition from beers collected all over the world.<br />
<br />
The project was founded in 2014 by [[User:Randogp|Gianpaolo Rando]] as an iteration of the [[Open-Food-DNA|Open Food DNA]] project. At the beginning, DNA was the only focus. Now we are expanding to other molecular analyses.<br />
<br />
<br><br />
<br />
__TOC__<br />
<br><br />
<br />
=Our goals=<br />
<br />
BeerDeCoded is a research project to draw a genetic map of beers. The public contributes to every step of this project: from securing funding to doing the experiment, from testing (and tasting) the samples to publishing the results. BeerDeCoded is doing-research-together, on the streets, in the bars and in an open laboratory.<br />
<br><br />
<br />
The main goals are the following:<br />
<br />
*To expand public understanding of molecular technologies (i.e. DNA sequencing) and their connection to culture.<br />
*To help non-scientist to study and analyse their beer samples at molecular level.<br />
*To motivate citizen scientists to generate an open access molecular dataset.<br />
*To understand the brewing process better.<br />
<br><br />
<br />
=Beer DNA?=<br />
Beer is a living beverage, and therefore it contains DNA. The DNA comes from its ingredients (hops, grains, yeast) and also from the microbes that were present on the ingredients and in the brewing environment. There are 1,000+ yeast varieties used for brewing and 200+ hops species, each one bearing a different DNA. Artisanal beer (craft beer) is trendy: microbreweries produce a large variety of beers, each one with its unique recipe and taste. It seems impossible to taste them all, so we are making a "tree of beers" based on their biochemical composition.<br />
<br><br />
<br />
=What now?=<br />
We are working on beer DNA extractions, sequencing and bioinformatics.<br />
* All the tasks are in this [[Trello]]: https://trello.com/b/NyG1Z7A7/beerdecoded-execution<br />
* Our last update (February 14th, 2017) for our Kickstarter backers: https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes/posts/1807332<br />
Want to help us? Drop us a note or join an #OpenHackuarium.<br />
<br><br />
<br />
=Events=<br />
We run BeerDeCoded workshops at Hackuarium or at public events. We can load a miniaturized laboratory on a cargo-bike and bring molecular analyses everywhere.<br />
<br />
If you want BeerDeCoded at your event, please contact gianpaolo.rando@swissdecode.ch<br />
<br />
Past events<br />
* Feb 11, 2017. Open Food Hackdays at EPFL https://hack.opendata.ch/project/71<br />
* Mar 24, 2016. Decoding Slovenian beers at Ljubljana c/o Biotehna http://www.meetup.com/BioTehna/events/228877465/<br />
* Mar 16, 2016. EPFL STIL16: Innovation and Technology exhibition. http://www.salon-stil.ch/index.php<br />
* Mar 08, 2016 [[Events:Entre_Bière_Hackers_et_ADN_Café_Scientifique_au_Collège_Rousseau |Entre bière, hackers et ADN]] le projet [[BeerDeCoded| BeerDeCoded]] participe au café scientifique du Collège Rousseau (Genève).<br />
* Feb 19, 2016. Swissnex San Francisco. A hands on night on beer microbiology, outreach and entrepreneurship open to Swiss alumni. www.swissnexsanfrancisco.org/event/beerdecoded/<br />
* Nov 28, 2015 [[DNA_Party_workchoppe_vol.2|A private party for our Kickstarter backers]] at Hackuarium<br />
* July 18, 2015 [[DNA_Party_workchoppe_vol.1|First test of the DNA Workchoppe]] at Hackuarium<br />
* Oct 30, 2015 LIFT Basel, "the future of food". Breakthrough and Workshop [[LIFTBasel15_BeerDeCoded_workshop|"Give them beers people talk about genomics]]<br />
* Oct 22, 2015 EXPO15 Milan, Swiss Pavillon<br />
* Sep 24, 2015 ScienceComm15, Solothurn<br />
<br />
*NCCR Retreat in Chemical Biology<br />
* GEW Geneva<br />
<br />
<br />
=How do we finance the project?=<br />
<br />
At LIFT15 conference in Geneva, we discussed the project during a science crowdfunding workshop.<br />
<br />
We then joined the first Science x Kickstarter hackathon in New York City (Feb 28, 2015) to draft a crowdfunding campaign for a pilot experiment.<br />
<br />
We have been successfully funded in 20 days in June 2015.<br />
<br />
After that, we attracted some sponsorship from local partners including Amstein SA, Swissnex San Francisco, Commune de Genève, ABO Valais.<br />
<br />
=How did we NOT finance the project?=<br />
<br />
We have our collection of refusals. We are proud of some applications and we list them here because they could be useful to other projects.<br />
<br />
* Science Outreach, Application to SNF Agora Fund, August 2015. https://docs.google.com/document/d/1xyid0vtE-mN6yEsQSlc9gNBAHjxdyplAlvuwkwPATT8/edit?usp=sharing<br />
* Business Model, Application to Illumina Accelerator, December 2015. Our kickstarter backers contributed to the application. https://docs.google.com/document/d/1y1QaitvAvTdMeRqt8gJDCp34fi-emPgQ-HG42aFEyAo/edit?usp=sharing<br />
<br />
<br />
=Impact=<br />
We only measured our outreach capacity during the Kickstarter campaign.<br />
<br />
During the Kickstarter campaign that run for the whole month of June 2015, there were 9,979 views (1,760 from Switzerland) on the kickstarter page. The related 2-min video containing simple technical aspects on beer DNA metagenomics was played 4,424 times (38% of plays completed). More than 2000 people interacted with us on social media, 723 people engaged in a discussion about the genetics of beer components, and 199 people devoted an average time of 5:21 min to answer 5 questions related to beer, DNA and decision making. The questions were “Tell me some beers you like”, “Why do you like these beers?”, “Where do you think taste comes from?”, “On the beer menu there are 20 beers you do not know, how do you choose?”, and “Imagine how the DNA that beers contain could help you make this choice”. During this pilot campaign, 123 people (including 6 breweries, 1 Swiss beer importer and 1 beer tasting association) financially contributed to the project, more than 20 people subscribed to a laboratory event to run a beer DNA analysis together and 10 joined the lab for a first full-day workshop in July 2015. BeerDeCoded was mentioned by 50+ magazines and blogs, including 3 Swiss daily newspapers (Le Temps, La Tribune de Genève, 24 Heures) and 2 TV programs on the Swiss national channel RTS (A bon entendeur, Le journal).<br />
<br />
On the 723 in-person conversations, we took notes on the first 107 conversations. When it comes to beer and DNA: 29 correctly understood it was a beer classification problem, 25 replied off-target or changed topic, 21 imagined an application to suggest beers (sommelier), 13 thought this was going to generate new beer recipes, 8 posed a strong objection, implying that this was not possible, 4 thought I was talking about GMO, 4 wondered about legal issues, 3 - including one journalist - thought I was talking about human DNA. In some conversations, people showed an intuition for important research topics, like genotype-environment interaction, feature selection, false discovery rate etc.., but this is more difficult to measure and probably less relevant. These conversations show that it is possible to exchange views and perspectives on genetic testing using beer DNA as an example.<br />
<br />
=Budget=<br />
Today BeerDeCoded runs on a voluntary basis. We pay reagents and services.<br />
We have funding for sequencing 96 beers. Rough project costs:<br />
<br />
* 500 CHF - Beer collection<br />
*1000 CHF - DNA purification (we extract DNA out of the beer)<br />
*5500 CHF - DNA amplification and multiplexing (we amplify the DNA, we label it with a ID code and we pool the labeled samples inside a sequencer)<br />
*3000 CHF - DNA sequencing.<br />
<br />
Other steps (manpower, bioinformatic analyses, data visualisation and communication) are done on a voluntary basis.<br />
<br><br />
<br />
=Team=<br />
<br />
Gianpaolo Rando, Ph.D.<br />
Director<br />
<br />
Jonathan Sobel, M.Sc.<br />
Bioinformatics<br />
<br />
Nicolas Rotman, Ph.D.<br />
Molecular biology<br />
<br />
Marco Maggiotti<br />
Software Engineer<br />
<br />
Vanessa Lorenzo<br />
Interaction design<br />
<br />
Alex Hantson, B.Sc.<br />
Digital marketing<br />
<br />
Luc Henry, Ph.D.<br />
Science outreach<br />
<br />
Gabrielle Salanon<br />
Brewing, molecular enology<br />
<br />
Brij Sahi, MBA<br />
Business development<br />
<br />
=Any question?=<br />
Contact us via [mailto:gianpaolo.rando@swissdecode.ch Gianpaolo Rando], [mailto:jsobel83@gmail.com Jonathan Sobel] or [mailto:luc.henry@hackuarium.ch Luc Henry].<br />
<br><br />
<br />
=BeerDeCoded on the Web=<br />
<br />
* [http://www.genome.beer Website]<br />
* [https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes Kickstarter Campaign]<br />
* [http://beerdecoded-presskit.strikingly.com/ Press Kit]<br />
<br />
=More on Social Media=<br />
* [https://twitter.com/beerdecoded on Twitter]<br />
* [https://www.facebook.com/BeerDeCoded on Facebook]<br />
<br />
=What Others Do=<br />
<br />
*[http://sdbn.org/2015/11/02/sdbn-november-30th-2015-event-dna-on-tap-the-genomics-of-beer/ WhiteLabs initiative]<br />
* Ava Winery Synthetic Wines, San Francisco. All wines are a collection of molecules including water, alcohol, sugars, acids, etc. Ava identifies those molecules and recombines them from scratch to recreate the wine at the molecular level. https://www.avawinery.com/<br />
<br />
[[Category:Projects]]<br />
[[Category:Open Food DNA]]<br />
[[Category:Work In Progress]]<br />
[[Category:BeerDeCoded]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=BeerDeCoded&diff=8682BeerDeCoded2017-03-11T18:25:19Z<p>Randogp: </p>
<hr />
<div>'''BeerDeCoded''' is a quest to decode the molecular composition from beers collected all over the world.<br />
<br />
The project was founded in 2014 by [[User:Randogp|Gianpaolo Rando]] as an iteration of the [[Open-Food-DNA|Open Food DNA]] project. At the beginning, DNA was the only focus. Now we are expanding to other molecular analyses.<br />
<br />
<br><br />
<br />
__TOC__<br />
<br><br />
<br />
=Our goals=<br />
<br />
BeerDeCoded is a research project to draw a genetic map of beers. The public contributes to every step of this project: from securing funding to doing the experiment, from testing (and tasting) the samples to publishing the results. BeerDeCoded is doing-research-together, on the streets, in the bars and in an open laboratory.<br />
<br><br />
<br />
The main goals are the following:<br />
<br />
*To expand public understanding of molecular technologies (i.e. DNA sequencing) and their connection to culture.<br />
*To help non-scientist to study and analyse their beer samples at molecular level.<br />
*To motivate citizen scientists to generate an open access molecular dataset.<br />
*To understand the brewing process better.<br />
<br><br />
<br />
=Beer DNA?=<br />
Beer is a living beverage, and therefore it contains DNA. The DNA comes from its ingredients (hops, grains, yeast) and also from the microbes that were present on the ingredients and in the brewing environment. There are 1,000+ yeast varieties used for brewing and 200+ hops species, each one bearing a different DNA. Artisanal beer (craft beer) is trendy: microbreweries produce a large variety of beers, each one with its unique recipe and taste. It seems impossible to taste them all, so we are making a "tree of beers" based on their biochemical composition.<br />
<br><br />
<br />
=What now?=<br />
We are working on beer DNA extractions, sequencing and bioinformatics.<br />
* All the tasks are in this [[Trello]]: https://trello.com/b/NyG1Z7A7/beerdecoded-execution<br />
* Our last update (February 14th, 2017) for our Kickstarter backers: https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes/posts/1807332<br />
Want to help us? Drop us a note or join an #OpenHackuarium.<br />
<br><br />
<br />
=Events=<br />
We run BeerDeCoded workshops at Hackuarium or at public events. We can load a miniaturized laboratory on a cargo-bike and bring molecular analyses everywhere.<br />
<br />
If you want BeerDeCoded at your event, please contact gianpaolo.rando@swissdecode.ch<br />
<br />
Past events<br />
* Feb 11, 2017. Open Food Hackdays at EPFL https://hack.opendata.ch/project/71<br />
* Mar 24, 2016. Decoding Slovenian beers at Ljubljana c/o Biotehna http://www.meetup.com/BioTehna/events/228877465/<br />
* Mar 16, 2016. EPFL STIL16: Innovation and Technology exhibition. http://www.salon-stil.ch/index.php<br />
* Mar 08, 2016 [[Events:Entre_Bière_Hackers_et_ADN_Café_Scientifique_au_Collège_Rousseau |Entre bière, hackers et ADN]] le projet [[BeerDeCoded| BeerDeCoded]] participe au café scientifique du Collège Rousseau (Genève).<br />
* Feb 19, 2016. Swissnex San Francisco. A hands on night on beer microbiology, outreach and entrepreneurship open to Swiss alumni. www.swissnexsanfrancisco.org/event/beerdecoded/<br />
* Nov 28, 2015 [[DNA_Party_workchoppe_vol.2|A private party for our Kickstarter backers]] at Hackuarium<br />
* July 18, 2015 [[DNA_Party_workchoppe_vol.1|First test of the DNA Workchoppe]] at Hackuarium<br />
* Oct 30, 2015 LIFT Basel, "the future of food". Breakthrough and Workshop [[LIFTBasel15_BeerDeCoded_workshop|"Give them beers people talk about genomics]]<br />
* Oct 22, 2015 EXPO15 Milan, Swiss Pavillon<br />
* Sep 24, 2015 ScienceComm15, Solothurn<br />
<br />
*NCCR Retreat in Chemical Biology<br />
* GEW Geneva<br />
<br />
<br />
=How do we finance the project?=<br />
<br />
At LIFT15 conference in Geneva, we discussed the project during a science crowdfunding workshop.<br />
<br />
We then joined the first Science x Kickstarter hackathon in New York City (Feb 28, 2015) to draft a crowdfunding campaign for a pilot experiment.<br />
<br />
We have been successfully funded in 20 days in June 2015.<br />
<br />
After that, we attracted some sponsorship from local partners including Amstein SA, Swissnex San Francisco, Commune de Genève, ABO Valais.<br />
<br />
=How did we NOT finance the project?=<br />
<br />
We have our collection of refusals. We are proud of some applications and we list them here because they could be useful to other projects.<br />
<br />
* Science Outreach, Application to SNF Agora Fund, August 2015. https://docs.google.com/document/d/1xyid0vtE-mN6yEsQSlc9gNBAHjxdyplAlvuwkwPATT8/edit?usp=sharing<br />
* Business Model, Application to Illumina Accelerator, December 2015. Our kickstarter backers contributed to the application. https://docs.google.com/document/d/1y1QaitvAvTdMeRqt8gJDCp34fi-emPgQ-HG42aFEyAo/edit?usp=sharing<br />
<br />
<br />
==Impact==<br />
We only measured our outreach capacity during the Kickstarter campaign.<br />
<br />
During the Kickstarter campaign that run for the whole month of June 2015, there were 9,979 views (1,760 from Switzerland) on the kickstarter page. The related 2-min video containing simple technical aspects on beer DNA metagenomics was played 4,424 times (38% of plays completed). More than 2000 people interacted with us on social media, 723 people engaged in a discussion about the genetics of beer components, and 199 people devoted an average time of 5:21 min to answer 5 questions related to beer, DNA and decision making. The questions were “Tell me some beers you like”, “Why do you like these beers?”, “Where do you think taste comes from?”, “On the beer menu there are 20 beers you do not know, how do you choose?”, and “Imagine how the DNA that beers contain could help you make this choice”. During this pilot campaign, 123 people (including 6 breweries, 1 Swiss beer importer and 1 beer tasting association) financially contributed to the project, more than 20 people subscribed to a laboratory event to run a beer DNA analysis together and 10 joined the lab for a first full-day workshop in July 2015. BeerDeCoded was mentioned by 50+ magazines and blogs, including 3 Swiss daily newspapers (Le Temps, La Tribune de Genève, 24 Heures) and 2 TV programs on the Swiss national channel RTS (A bon entendeur, Le journal).<br />
<br />
On the 723 in-person conversations, we took notes on the first 107 conversations. When it comes to beer and DNA: 29 correctly understood it was a beer classification problem, 25 replied off-target or changed topic, 21 imagined an application to suggest beers (sommelier), 13 thought this was going to generate new beer recipes, 8 posed a strong objection, implying that this was not possible, 4 thought I was talking about GMO, 4 wondered about legal issues, 3 - including one journalist - thought I was talking about human DNA. In some conversations, people showed an intuition for important research topics, like genotype-environment interaction, feature selection, false discovery rate etc.., but this is more difficult to measure and probably less relevant. These conversations show that it is possible to exchange views and perspectives on genetic testing using beer DNA as an example.<br />
<br />
=Budget=<br />
Today BeerDeCoded runs on a voluntary basis. We pay reagents and services.<br />
We have funding for sequencing 96 beers. Rough project costs:<br />
<br />
* 500 CHF - Beer collection<br />
*1000 CHF - DNA purification (we extract DNA out of the beer)<br />
*5500 CHF - DNA amplification and multiplexing (we amplify the DNA, we label it with a ID code and we pool the labeled samples inside a sequencer)<br />
*3000 CHF - DNA sequencing.<br />
<br />
Other steps (manpower, bioinformatic analyses, data visualisation and communication) are done on a voluntary basis.<br />
<br><br />
<br />
=Team=<br />
<br />
Gianpaolo Rando, Ph.D.<br />
Director<br />
<br />
Jonathan Sobel, M.Sc.<br />
Bioinformatics<br />
<br />
Nicolas Rotman, Ph.D.<br />
Molecular biology<br />
<br />
Marco Maggiotti<br />
Software Engineer<br />
<br />
Vanessa Lorenzo<br />
Interaction design<br />
<br />
Alex Hantson, B.Sc.<br />
Digital marketing<br />
<br />
Luc Henry, Ph.D.<br />
Science outreach<br />
<br />
Gabrielle Salanon<br />
Brewing, molecular enology<br />
<br />
Brij Sahi, MBA<br />
Business development<br />
<br />
=Any question?=<br />
Contact us via [mailto:gianpaolo.rando@swissdecode.ch Gianpaolo Rando], [mailto:jsobel83@gmail.com Jonathan Sobel] or [mailto:luc.henry@hackuarium.ch Luc Henry].<br />
<br><br />
<br />
=BeerDeCoded on the Web=<br />
<br />
* [http://www.genome.beer Website]<br />
* [https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes Kickstarter Campaign]<br />
* [http://beerdecoded-presskit.strikingly.com/ Press Kit]<br />
<br />
=More on Social Media=<br />
* [https://twitter.com/beerdecoded on Twitter]<br />
* [https://www.facebook.com/BeerDeCoded on Facebook]<br />
<br />
=What Others Do=<br />
<br />
*[http://sdbn.org/2015/11/02/sdbn-november-30th-2015-event-dna-on-tap-the-genomics-of-beer/ WhiteLabs initiative]<br />
* Ava Winery Synthetic Wines, San Francisco. All wines are a collection of molecules including water, alcohol, sugars, acids, etc. Ava identifies those molecules and recombines them from scratch to recreate the wine at the molecular level. https://www.avawinery.com/<br />
<br />
[[Category:Projects]]<br />
[[Category:Open Food DNA]]<br />
[[Category:Work In Progress]]<br />
[[Category:BeerDeCoded]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=BeerDeCoded&diff=8681BeerDeCoded2017-03-11T18:02:14Z<p>Randogp: /* What now? */</p>
<hr />
<div>'''BeerDeCoded''' is a quest to sequence the DNA found in 1,000 beers from all over the world.<br />
<br />
The project is led by [[Hackuarium]] member [[User:Randogp|Gianpaolo Rando]] and is an iteration of the [[Open-Food-DNA|Open Food DNA]] project.<br />
<br />
<br><br />
<br />
__TOC__<br />
<br><br />
<br />
=Our goals=<br />
<br />
BeerDeCoded is a research project to draw a genetic map of beers. The public contributes to every step of this project: from securing funding to doing the experiment, from testing (and tasting) the samples to publishing the results. BeerDeCoded is doing-research-together, on the streets, in the bars and in an open laboratory.<br />
<br><br />
<br />
The main goals are the following:<br />
<br />
*Democratise DNA analyses<br />
*Demystify and explain genetic data<br />
*Produce an open access knowledge database<br />
*Understand the brewing process better<br />
<br><br />
<br />
=Beer DNA?=<br />
Beer is alive, and therefore it contains DNA. The DNA comes from its ingredients (hops, grains, yeast) and from the microbes that were present on the ingredients and in the brewing environment. There are 1,000+ yeast varieties used for brewing and 200+ hops species, each one bearing a different DNA. Artisanal beer (craft beer) is trendy: microbreweries produce a large variety of beers, each one with its unique recipe and taste. It seems impossible to taste them all, so we are making a "tree of beers" based on their biochemical composition.<br />
<br><br />
<br />
=What now?=<br />
We are working on beer DNA extractions, sequencing and bioinformatics.<br />
* All the tasks are in this [[Trello]]: https://trello.com/b/NyG1Z7A7/beerdecoded-execution<br />
* Our last update (February 14th, 2017) for our Kickstarter backers: https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes/posts/1807332<br />
Want to help us? Drop us a note or join an #OpenHackuarium.<br />
<br><br />
<br />
=Events=<br />
We run BeerDeCoded workshops at Hackuarium or at public events. We can load a miniaturized laboratory on a cargo-bike and bring molecular analyses everywhere.<br />
<br />
If you want BeerDeCoded at your event, please contact gianpaolo.rando@swissdecode.ch<br />
<br />
Past events<br />
* Feb 11, 2017. Open Food Hackdays at EPFL https://hack.opendata.ch/project/71<br />
* Mar 24, 2016. Decoding Slovenian beers at Ljubljana c/o Biotehna http://www.meetup.com/BioTehna/events/228877465/<br />
* Mar 16, 2016. EPFL STIL16: Innovation and Technology exhibition. http://www.salon-stil.ch/index.php<br />
* Mar 08, 2016 [[Events:Entre_Bière_Hackers_et_ADN_Café_Scientifique_au_Collège_Rousseau |Entre bière, hackers et ADN]] le projet [[BeerDeCoded| BeerDeCoded]] participe au café scientifique du Collège Rousseau (Genève).<br />
* Feb 19, 2016. Swissnex San Francisco. A hands on night on beer microbiology, outreach and entrepreneurship open to Swiss alumni. www.swissnexsanfrancisco.org/event/beerdecoded/<br />
* Nov 28, 2015 [[DNA_Party_workchoppe_vol.2|A private party for our Kickstarter backers]] at Hackuarium<br />
* July 18, 2015 [[DNA_Party_workchoppe_vol.1|First test of the DNA Workchoppe]] at Hackuarium<br />
* Oct 30, 2015 LIFT Basel, "the future of food". Breakthrough and Workshop [[LIFTBasel15_BeerDeCoded_workshop|"Give them beers people talk about genomics]]<br />
* Oct 22, 2015 EXPO15 Milan, Swiss Pavillon<br />
* Sep 24, 2015 ScienceComm15, Solothurn<br />
<br />
*NCCR Retreat in Chemical Biology<br />
* GEW Geneva<br />
<br />
<br />
=How do we finance the project?=<br />
<br />
At LIFT15 conference in Geneva, we discussed the project during a science crowdfunding workshop.<br />
<br />
We then joined the first Science x Kickstarter hackathon in New York City (Feb 28, 2015) to craft a crowdfunding campaign for a pilot experiment.<br />
<br />
We have been successfully funded in 20 days in June 2015.<br />
<br />
After that, we attracted some sponsorship from local partners.<br />
<br />
==Impact==<br />
We only measured our outreach capacity during the Kickstarter campaign.<br />
<br />
During the Kickstarter campaign that run for the whole month of June 2015, there were 9,979 views (1,760 from Switzerland) on the kickstarter page. The related 2-min video containing simple technical aspects on beer DNA metagenomics was played 4,424 times (38% of plays completed). More than 2000 people interacted with us on social media, 723 people engaged in a discussion about the genetics of beer components, and 199 people devoted an average time of 5:21 min to answer 5 questions related to beer, DNA and decision making. The questions were “Tell me some beers you like”, “Why do you like these beers?”, “Where do you think taste comes from?”, “On the beer menu there are 20 beers you do not know, how do you choose?”, and “Imagine how the DNA that beers contain could help you make this choice”. During this pilot campaign, 123 people (including 6 breweries, 1 Swiss beer importer and 1 beer tasting association) financially contributed to the project, more than 20 people subscribed to a laboratory event to run a beer DNA analysis together and 10 joined the lab for a first full-day workshop in July 2015. BeerDeCoded was mentioned by 50+ magazines and blogs, including 3 Swiss daily newspapers (Le Temps, La Tribune de Genève, 24 Heures) and 2 TV programs on the Swiss national channel RTS (A bon entendeur, Le journal).<br />
<br />
On the 723 in-person conversations, we took notes on the first 107 conversations. When it comes to beer and DNA: 29 correctly understood it was a beer classification problem, 25 replied off-target or changed topic, 21 imagined an application to suggest beers (sommelier), 13 thought this was going to generate new beer recipes, 8 posed a strong objection, implying that this was not possible, 4 thought I was talking about GMO, 4 wondered about legal issues, 3 - including one journalist - thought I was talking about human DNA. In some conversations, people showed an intuition for important research topics, like genotype-environment interaction, feature selection, false discovery rate etc.., but this is more difficult to measure and probably less relevant. These conversations show that it is possible to exchange views and perspectives on genetic testing using beer DNA as an example.<br />
<br />
=Budget=<br />
Today BeerDeCoded runs on a voluntary basis. We pay reagents and services.<br />
We have funding for sequencing 96 beers. Rough project costs:<br />
<br />
* 500 CHF - Beer collection<br />
*1000 CHF - DNA purification (we extract DNA out of the beer)<br />
*5500 CHF - DNA amplification and multiplexing (we amplify the DNA, we label it with a ID code and we pool the labeled samples inside a sequencer)<br />
*3000 CHF - DNA sequencing.<br />
<br />
Other steps (manpower, bioinformatic analyses, data visualisation and communication) are done on a voluntary basis.<br />
<br><br />
<br />
=Team=<br />
<br />
Gianpaolo Rando leads the Open Food DNA project. He holds a Ph.D in biotechnology and has 10 years of experience at the interface between genomics and nutrition. He is a proud member of Hackurium, has a passion for food and drinks and wants to expand the project to beer and other beverages (fb.com/BeerDeCoded).<br />
<br />
Jonathan Sobel<br />
<br />
Nicolas<br />
<br />
Luc Henry holds a DPhil in chemical biology and has a genuine interest in the impact of new technologies on society, in particular in the fields of biotechnology and health. He is the co-founder of Hackuarium and wants to promote an open approach to science and innovation.<br />
<br />
=Any question?=<br />
Contact us via [mailto:gianpaolo.rando@swissdecode.ch Gianpaolo Rando], [mailto:jsobel83@gmail.com Jonathan Sobel] or [mailto:luc.henry@hackuarium.ch Luc Henry].<br />
<br><br />
<br />
=BeerDeCoded on the Web=<br />
<br />
* [http://www.genome.beer Website]<br />
* [https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes Kickstarter Campaign]<br />
* [http://beerdecoded-presskit.strikingly.com/ Press Kit]<br />
<br />
=More on Social Media=<br />
* [https://twitter.com/beerdecoded on Twitter]<br />
* [https://www.facebook.com/BeerDeCoded on Facebook]<br />
<br />
=What Others Do=<br />
<br />
*[http://sdbn.org/2015/11/02/sdbn-november-30th-2015-event-dna-on-tap-the-genomics-of-beer/ WhiteLabs initiative]<br />
<br />
[[Category:Projects]]<br />
[[Category:Open Food DNA]]<br />
[[Category:Work In Progress]]<br />
[[Category:BeerDeCoded]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=BeerDeCoded&diff=8456BeerDeCoded2017-02-13T21:43:41Z<p>Randogp: </p>
<hr />
<div>'''BeerDeCoded''' is a quest to sequence the DNA found in 1,000 beers from all over the world.<br />
<br />
The project is led by [[Hackuarium]] member [[User:Randogp|Gianpaolo Rando]] and is an iteration of the [[Open-Food-DNA|Open Food DNA]] project.<br />
<br />
<br><br />
<br />
__TOC__<br />
<br><br />
<br />
=Our goals=<br />
<br />
BeerDeCoded is a research project to draw a genetic map of beers. The public contributes to every step of this project: from securing funding to doing the experiment, from testing (and tasting) the samples to publishing the results. BeerDeCoded is doing-research-together, on the streets, in the bars and in an open laboratory.<br />
<br><br />
<br />
The main goals are the following:<br />
<br />
*Democratise DNA analyses<br />
*Demystify and explain genetic data<br />
*Produce an open access knowledge database<br />
*Understand the brewing process better<br />
<br><br />
<br />
=Beer DNA?=<br />
Beer is alive, and therefore it contains DNA. The DNA comes from its ingredients (hops, grains, yeast) and from the microbes that were present on the ingredients and in the brewing environment. There are 1,000+ yeast varieties used for brewing and 200+ hops species, each one bearing a different DNA. Artisanal beer (craft beer) is trendy: microbreweries produce a large variety of beers, each one with its unique recipe and taste. It seems impossible to taste them all, so we are making a "tree of beers" based on their biochemical composition.<br />
<br><br />
<br />
=What now?=<br />
We received the Kickstarter payment and we are actively working on beer DNA extractions, sequencing and bioinformatics.<br />
* All the tasks are in this [[Trello]]: https://trello.com/b/NyG1Z7A7/beerdecoded-execution<br />
* Our last update (October 27, 2015) for our Kickstarter backers: https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes/posts/1394351<br />
Want to help us? Drop us a note or join an #OpenHackuarium.<br />
<br><br />
<br />
=Events=<br />
We run BeerDeCoded workshops at Hackuarium or at public events. We can load a miniaturized laboratory on a cargo-bike and bring molecular analyses everywhere.<br />
<br />
If you want BeerDeCoded at your event, please contact gianpaolo.rando@swissdecode.ch<br />
<br />
Past events<br />
* Feb 11, 2017. Open Food Hackdays at EPFL https://hack.opendata.ch/project/71<br />
* Mar 24, 2016. Decoding Slovenian beers at Ljubljana c/o Biotehna http://www.meetup.com/BioTehna/events/228877465/<br />
* Mar 16, 2016. EPFL STIL16: Innovation and Technology exhibition. http://www.salon-stil.ch/index.php<br />
* Mar 08, 2016 [[Events:Entre_Bière_Hackers_et_ADN_Café_Scientifique_au_Collège_Rousseau |Entre bière, hackers et ADN]] le projet [[BeerDeCoded| BeerDeCoded]] participe au café scientifique du Collège Rousseau (Genève).<br />
* Feb 19, 2016. Swissnex San Francisco. A hands on night on beer microbiology, outreach and entrepreneurship open to Swiss alumni. www.swissnexsanfrancisco.org/event/beerdecoded/<br />
* Nov 28, 2015 [[DNA_Party_workchoppe_vol.2|A private party for our Kickstarter backers]] at Hackuarium<br />
* July 18, 2015 [[DNA_Party_workchoppe_vol.1|First test of the DNA Workchoppe]] at Hackuarium<br />
* Oct 30, 2015 LIFT Basel, "the future of food". Breakthrough and Workshop [[LIFTBasel15_BeerDeCoded_workshop|"Give them beers people talk about genomics]]<br />
* Oct 22, 2015 EXPO15 Milan, Swiss Pavillon<br />
* Sep 24, 2015 ScienceComm15, Solothurn<br />
<br />
*NCCR Retreat in Chemical Biology<br />
* GEW Geneva<br />
<br />
<br />
=How do we finance the project?=<br />
<br />
At LIFT15 conference in Geneva, we discussed the project during a science crowdfunding workshop.<br />
<br />
We then joined the first Science x Kickstarter hackathon in New York City (Feb 28, 2015) to craft a crowdfunding campaign for a pilot experiment.<br />
<br />
We have been successfully funded in 20 days in June 2015.<br />
<br />
After that, we attracted some sponsorship from local partners.<br />
<br />
==Impact==<br />
We only measured our outreach capacity during the Kickstarter campaign.<br />
<br />
During the Kickstarter campaign that run for the whole month of June 2015, there were 9,979 views (1,760 from Switzerland) on the kickstarter page. The related 2-min video containing simple technical aspects on beer DNA metagenomics was played 4,424 times (38% of plays completed). More than 2000 people interacted with us on social media, 723 people engaged in a discussion about the genetics of beer components, and 199 people devoted an average time of 5:21 min to answer 5 questions related to beer, DNA and decision making. The questions were “Tell me some beers you like”, “Why do you like these beers?”, “Where do you think taste comes from?”, “On the beer menu there are 20 beers you do not know, how do you choose?”, and “Imagine how the DNA that beers contain could help you make this choice”. During this pilot campaign, 123 people (including 6 breweries, 1 Swiss beer importer and 1 beer tasting association) financially contributed to the project, more than 20 people subscribed to a laboratory event to run a beer DNA analysis together and 10 joined the lab for a first full-day workshop in July 2015. BeerDeCoded was mentioned by 50+ magazines and blogs, including 3 Swiss daily newspapers (Le Temps, La Tribune de Genève, 24 Heures) and 2 TV programs on the Swiss national channel RTS (A bon entendeur, Le journal).<br />
<br />
On the 723 in-person conversations, we took notes on the first 107 conversations. When it comes to beer and DNA: 29 correctly understood it was a beer classification problem, 25 replied off-target or changed topic, 21 imagined an application to suggest beers (sommelier), 13 thought this was going to generate new beer recipes, 8 posed a strong objection, implying that this was not possible, 4 thought I was talking about GMO, 4 wondered about legal issues, 3 - including one journalist - thought I was talking about human DNA. In some conversations, people showed an intuition for important research topics, like genotype-environment interaction, feature selection, false discovery rate etc.., but this is more difficult to measure and probably less relevant. These conversations show that it is possible to exchange views and perspectives on genetic testing using beer DNA as an example.<br />
<br />
=Budget=<br />
Today BeerDeCoded runs on a voluntary basis. We pay reagents and services.<br />
We have funding for sequencing 96 beers. Rough project costs:<br />
<br />
* 500 CHF - Beer collection<br />
*1000 CHF - DNA purification (we extract DNA out of the beer)<br />
*5500 CHF - DNA amplification and multiplexing (we amplify the DNA, we label it with a ID code and we pool the labeled samples inside a sequencer)<br />
*3000 CHF - DNA sequencing.<br />
<br />
Other steps (manpower, bioinformatic analyses, data visualisation and communication) are done on a voluntary basis.<br />
<br><br />
<br />
=Team=<br />
<br />
Gianpaolo Rando leads the Open Food DNA project. He holds a Ph.D in biotechnology and has 10 years of experience at the interface between genomics and nutrition. He is a proud member of Hackurium, has a passion for food and drinks and wants to expand the project to beer and other beverages (fb.com/BeerDeCoded).<br />
<br />
Jonathan Sobel<br />
<br />
Nicolas<br />
<br />
Luc Henry holds a DPhil in chemical biology and has a genuine interest in the impact of new technologies on society, in particular in the fields of biotechnology and health. He is the co-founder of Hackuarium and wants to promote an open approach to science and innovation.<br />
<br />
=Any question?=<br />
Contact us via [mailto:gianpaolo.rando@swissdecode.ch Gianpaolo Rando], [mailto:jsobel83@gmail.com Jonathan Sobel] or [mailto:luc.henry@hackuarium.ch Luc Henry].<br />
<br><br />
<br />
=BeerDeCoded on the Web=<br />
<br />
* [http://www.genome.beer Website]<br />
* [https://www.kickstarter.com/projects/489252126/beerdecoded-the-1000-beer-genomes Kickstarter Campaign]<br />
* [http://beerdecoded-presskit.strikingly.com/ Press Kit]<br />
<br />
=More on Social Media=<br />
* [https://twitter.com/beerdecoded on Twitter]<br />
* [https://www.facebook.com/BeerDeCoded on Facebook]<br />
<br />
=What Others Do=<br />
<br />
*[http://sdbn.org/2015/11/02/sdbn-november-30th-2015-event-dna-on-tap-the-genomics-of-beer/ WhiteLabs initiative]<br />
<br />
[[Category:Projects]]<br />
[[Category:Open Food DNA]]<br />
[[Category:Work In Progress]]<br />
[[Category:BeerDeCoded]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20161024_Board_meeting&diff=803620161024 Board meeting2016-10-24T16:16:59Z<p>Randogp: /* BeerDeCoded */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Monday, October 24th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections? NO<br />
<br />
==Point 1: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee -> Luc (up to end July, then for 3 months) -> DONE<br />
<br><br />
-> Send a summary with membership fees and conditions -> Send with invitation to General Assembly on Friday 28th October.<br />
<br><br />
-> Ask each members to find an idea for a 3h workshop in 2017 (Yann: Who's idea? Plan to implement it?) -> Send with invitation to General Assembly <br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue) <br><br />
<br />
To board members: <br><br />
-> Next board meeting is on 28th November <br><br />
-> Identify and break down tasks for the board (2 hours a week + list of tasks) -> DONE [[Board | here]]<br><br />
-> Each of the board members think of tasks they can give to members and supervise -> NOT DONE <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Write profile for 2 interns, including tasks and skills -> WHO? <br><br />
-> Update list of projects, identify those that need help -> WHO? <br><br />
-> Every board member sends an email/call to 2-3 active projects -> WHO?<br><br />
*check where they are<br />
*what is stopping them<br />
*what would they need to come more often<br />
*report at the next board meeting<br />
-> Sam gets RFID stickers to attach to the main card. <br><br />
-> Sam makes primary twitter channel and see how many unfollow (1month) <br><br />
-> Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) until the next General Assembly <br><br />
<br />
==Point 2: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
==== Workshops ====<br />
Need a model to standardize and organize workshops execution. <br><br />
Can we get a zone on the wiki or the website accessible only to members where the can get workshop lists with protocols? <br><br />
How is the income shared ? <br><br />
<br />
==== Experiment Shelf ====<br />
We talked about this several times but it would be time to set it up. We could organize a shelf were boxes contain experiments you can do on your own for a small price (2 to 10 bucks).<br />
<br />
==== Project Proposition ====<br />
A thing I realize when looking at how octanis worked is that they proposed to people small tasks to carry part of a bigger project. <br><br />
I have ideas, I have to focus my time on other things. Can we make a project proposal page to our members ? <br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Yann (5 min)<br><br />
<br />
:: Take home from the co-creation for UC<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
*Zone built<br />
*Being furnished<br />
*Documentation to be pushed forward (1 short blog post)<br />
*Plan first experiments<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
<br />
Good samples (39) Process launched at UniL Genomic Facility. Next steps for them 1) purif 2) QC test 3) 2nd PCR 4) QC test 5) actual sequencing <br><br />
<br />
Bad samples: Isabelle confirmed on nanodrop UniL that the DNA is dirty (contaminated). No more progress as good weather for her first project. <br><br />
<br />
Next steps at Hackuarium:<br />
* train new people to do the tests (pipetting workshop already planned)<br />
* news on the DIY nanodrop?<br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
===Hybridoa===<br />
<br />
Update from Vanessa (5min) <br><br />
<br />
Yann: What is Hybridoa? <br><br />
<br />
Workshops UC Calendar<br><br />
<br />
===Darty Monkeys===<br />
Update from Vanessa (5min)<br><br />
Yann: What is this again ?<br><br />
Luc: How is it different from Hybridoa?<br><br />
<br />
==Point 3: Finance and Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application was submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] on October 15th.<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br><br />
Q (Yann): Should we also aim at companies ? Nestlé, Thermo, Merks, Roche ... Some might not be "delicate" since people might become unhappy if they see Bayer or DARPA as cash income<br />
A (Luc): What do we need cash for? We need specific projects to apply for anything. I am ok with any source that is not cigarettes or military. Sponsors and funding: it takes a lot of time to built trust. Anyone welcome to go ahead and start building relationships.<br />
<br />
==Point 4: Future of the association==<br />
<br />
Luc (10 min)<br />
<br />
After a discussion on Wednesday 5th October, there is a clear need to realign visions and plan the future of the association.<br><br />
<br />
What I suggest is to discuss tonight WHAT we need to work on, and who will take responsibility. Then we can present something at the General Assembly in December, to be voted in early 2017 in a extraordinary meeting of the members.<br />
<br />
Anyone else wants to add something?<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
:<li>'''2016.11.02''' Wednesday 19:30-22:00: [[Openhackuarium_vol._122 | Openhackuarium vol. 122]] Epidemium and Echopen: when Open Source meets Pharma and Medtech<br />
<br />
===Future events===<br />
<br />
'''Molecular Biology day/evening'''<br />
<br />
Luc (3 min)<br />
<br />
Move to 2017 <br />
*WHEN? '''2017''' Wednesday 19:30-22:00<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
==Point 6: Other Events==<br />
<br />
* Gianpaolo's pipette & beyond workshop (WHEN?)<br />
<br />
* Ethics discussion (2017, in collaboration with Bruno Strasser UNIGE)<br />
<br />
* DIYbio EU meeting, 10-13 May 2017, organised with Bruno Strasser (University of Geneva), a research symposium about DIYbio before the DIYbio EU meeting.<br />
<br />
==Point 7: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
Luc: can I get my email address back? ;)<br />
<br />
==Point 8: Hacktion==<br />
<br />
<br />
==Point 9: Replication==<br />
<br />
* Open nanodrop<br />
* Food computer<br />
*?<br />
<br />
==Point 10: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20161024_Board_meeting&diff=801820161024 Board meeting2016-10-24T09:29:32Z<p>Randogp: /* Point 1: Actionables */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Monday, October 24th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections? NO<br />
<br />
==Point 1: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee using [http://www.smallinvoice.ch/CH/en/home/Create-and-manage-invoices-and-offers-for-free.html Smallinvoice.ch] -> Luc (up to end July, then for 3 months) -> NOT DONE<br />
<br><br />
-> Send a summary with membership fees and conditions -> NOT DONE<br />
<br><br />
-> make a slide to insist at OH that people should talk about the place and advertise for it and become members (and how) -> DONE but needs to be DONE AGAIN<br />
-> Ask each members to find an idea for a 3h workshop in 2017 (Yann: Who's idea? Plan to implement it ?)<br><br />
<br />
To board members: <br><br />
-> Identify and break down tasks for the board <br><br />
-> Each of the board members think of tasks they can give to members and supervise <br><br />
-> Email board member and ask for dedication (2 hours a week + list of tasks) -> Luc to present at this board meeting <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Anne-Laure makes a list of people to invite in 2017<br><br />
-> Implement [https://en.wikipedia.org/wiki/Performance_indicator key performance indicators] until the next General Assembly on Wednesday 14th December 2016 (Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) <br><br />
-> Profile for 2 interns, including tasks and skills <br><br />
-> List of projects that need help <br><br />
-> Next board meetings 19th of Sept. 24th October, 28th November -> Luc sent a reminder on 31st August to board members<br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue). <br><br />
<br />
In general: <br><br />
-> Every board member sends an email/call to 2-3 active projects and check where they are and report at the next board meeting, what is stopping you, what would you need to come more often <br><br />
-> Gianpaolo will check the price for distilled water DONE 5 liters / 3 CHF (Migros). Bought one.<br />
::(Yann: We can also go and fill tanks times to times at EPFL for the time being) <br><br />
-> Luc asks Cyanoguard what he expects from the lab. NOT DONE, too late ? Feedback maybe ?<br><br />
-> Make sure we have a list of tasks to get people on board -> post-its with projects and tasks so that people can sign-up. <br><br />
-> Sam gets RFID stickers to attach to the main card. <br><br />
-> Sam makes primary twitter channel and see how many unfollow (1month) <br><br />
-> Luc sends Pi workshop tweets. DONE <br><br />
-> Yann sends Facebook message with Hackuarium. DONE <br><br />
<br />
==Point 2: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
==== Workshops ====<br />
Need a model to standardize and organize workshops execution. <br><br />
Can we get a zone on the wiki or the website accessible only to members where the can get workshop lists with protocols? <br><br />
How is the income shared ? <br><br />
<br />
==== Experiment Shelf ====<br />
We talked about this several times but it would be time to set it up. We could organize a shelf were boxes contain experiments you can do on your own for a small price (2 to 10 bucks).<br />
<br />
==== Project Proposition ====<br />
A thing I realize when looking at how octanis worked is that they proposed to people small tasks to carry part of a bigger project. <br><br />
I have ideas, I have to focus my time on other things. Can we make a project proposal page to our members ? <br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Luc (5 min)<br><br />
<br />
:: Take home from the co-creation for UC<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
*Zone built<br />
*Being furnished<br />
*Documentation to be pushed forward.<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
===Hybridoa===<br />
Yann: What is Hybridoa? <br><br />
Update from Vanessa (5min) <br><br />
Workshops UC Calendar<br><br />
<br />
===Darty Monkeys===<br />
Update from Vanessa (5min)<br />
Yann: What is this again ?<br><br />
<br />
==Point 3: Finance and Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application was submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] on October 15th.<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br />
(Yann: Checked the list, that's a large pool, good. Should we also aim at companies ? Nestlé, Thermo, Merks, Roche ... Some might not be "delicate" since people might become unhappy if they see Bayer or DAPRA as cash income)<br />
<br />
==Point 4: Future partnerships==<br />
<br />
===Mike Horn pole2pole===<br />
<br />
===Joint Outreach with SIB===<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
===Molecular Biology day/evening===<br />
<br />
Luc (3 min)<br />
<br />
Was moved to mid-october so we can advertise for it at the [[Openhackuarium_vol._116 | Openhackuarium vol. 116]].<br />
<br />
*WHEN? '''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]]<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
==Point 6: Other Events==<br />
<br />
* Gianpaolo's pipette & beyond workshop (to be announced on October 19th.<br />
<br />
* Ethics discussion (Luc)<br />
<br />
==Point 7: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
==Point 8: Hacktion==<br />
<br />
<br />
==Point 9: Replication==<br />
<br />
<br />
==Point 10: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Main_Page&diff=7976Main Page2016-09-30T08:08:35Z<p>Randogp: /* Up-to-date list of instruments we still need */</p>
<hr />
<div>Welcome to the wiki of [http://www.hackuarium.ch/ Hackuarium] community.<br />
<br />
At Hackuarium, we want to bring biology (and biologists) to the world, and the real world back to biology. Our laboratory is an excuse to meet and discuss, build and develop ideas in a neutral, open, noncompetitive and not-for-profit environment.<br />
<br />
<div><br />
''[[Hackuarium | Cliquez ici pour accéder à la version française de ce wiki.]]'' <br />
</div><br />
<br />
This page was launched during our [[1st Ever Hackuarium Wiki Sprint]] that took place on 7 December 2014. As you might notice quickly, it is a work-in-progress.<br />
<br />
Scroll down and find out what this is all about!<br />
<br />
__TOC__<br />
<br />
=DIY Bio=<br />
<br />
==What is DIYbio?==<br />
<br />
DIYbio (Do-It-Yourself biology) is a movement that wants to free the practice of biological research and innovation from the institutional and industrial laboratories. Pursued both by amateurs and professional biologists, DIYbio is developing low-cost and low-tech solutions to problems identified by the community.<br />
<br />
The movement is characterised by an extremely diverse set of practices and participants. Some call themselves 'biohackers', in reference to the hacker culture.<br />
<br />
[http://DIYbio.org DIYbio.org] and [http://DIYbio.eu DIYbio.eu] are the portals of the international and european communities, respectively.<br />
<br />
For more information, visit the [http://www.wikipedia.org Wikipedia] page dedicated to [https://en.wikipedia.org/wiki/DIYbio DIYbio].<br />
<br><br />
<br />
=Hackuarium=<br />
<br />
Hackuarium is a not-for-profit association aiming at democratizing science through public engagement.<br />
<br><br />
<br />
Our laboratory in Renens (Switzerland) is open to anyone sharing the values of the association and who is dedicated to follow the [http://diybio.org/codes/draft-diybio-code-of-ethics-from-european-congress/ DIYbio Europe ethical guidelines].<br />
<br />
Our projects are initiated and carried out by scientists as well as non-scientists from a variety of background. They are passionate about tinkering with biology in particular, and technology in general. Some are engineers, architects, designers, IT and computer scientists or retired professionals, but others have no scientific education. They are mostly citizens interested in open and participatory research and innovation, outside the contrains of traditional institutions.<br />
<br />
Hackuarium members want to investigate new ways of carrying out interdisciplinary research and innovation, by making their results accessible (low-cost), simple and easily reproducible (low-tech) and by promoting an open source philosophy.<br />
<br />
Hackuarium is proudly using infrastructures provided by [http://www.univercite.ch UniverCité], an unconventional innovation hub opened in May 2014. Our equipment is mostly upcycled material from institutions and industries from western Switzerland.<br />
<br />
=Who we are=<br />
<br />
Renens is a hopping hub - and our members reflect the diversity that is in and around Lausanne/Renens.<br />
<br><br />
[[People | Check us out]], and find out what makes Hackuarium a good mix.<br />
<br><br />
<br />
==Committee==<br />
<br />
The current [[Board|committee]] is composed of the following people:<br />
<br />
* President & Treasurer: [[User:luchenry | Luc Henry]]<br />
* Secretary: [[User:mmastern | Michal Masternak]]<br />
* Lab Manager: [[User:hekkcess | Yann Pierson]]<br />
* Information technology & Infrastructure: [[User:Sam | Sam Sulaimanov]]<br />
* Art and Design coordinator: [[User:Vlorenzo | Vanessa Lorenzo]]<br />
* Talent scout and evangelist: [[User:Randogp | Gianpaolo Rando]]<br />
* Events and coordinator: [[User:Alpittet | Anne-Laure Pittet]]<br />
* Member: Ana Roldan<br />
<br />
The Agenda of the last board meeting can be found [[20160818_Board_meeting | here]].<br><br><br />
<br />
==Members and Membership==<br />
<br />
Hackuarium operates on a membership basis at this current time.<br />
<br />
The monthly membership fee is 20 CHF. It gives 24/7 access to the lab.<br />
<br />
Please ask a member of the board if you want to join as a member.<br />
<br />
All events are open to anyone, including non-members.<br />
<br />
==Friends of Hackuarium==<br />
<br />
Spaces:<br />
::<li>[http://www.univercite.ch UniverCité]<br />
::<li>[http://lapaillasse.org La Paillasse, Paris]<br />
::<li>[https://lapaillassaone.wordpress.com Paillasse Saône, Lyon]<br />
<br />
Projects:<br />
::<li>[[Exodes_Urbains | Exodes Urbains]]<br />
::<li>[http://echopen.org/index.php?title=Main_Page EchOpen]<br />
<br />
==Where we are==<br />
Hackuarium is one of the association active at UniverCité, an innovation space under construction since May 2014 in the IRL building in Renens. We have documented our working environment as it has evolved.<br><br />
As of December 2015, UniverCité regroups a laboratory, a workshop and co-working space.<br><br />
<br><br />
<br />
=Events=<br />
<br />
<br><br />
<big>'''[[Open_Hackuarium | OpenHackuarium]]: Every Wednesday, 19:00-22:00'''</big><br />
<br />
Our lab and space are open to the public. Please come and have a chat! And if you have any questions [mailto:hello@hackuarium.ch get in touch!]<br />
<br />
==Upcoming Hackuarium Events==<br />
<br><br />
:<li>'''2016.12.14''' Wednesday 19:30-20:30: [[Openhackuarium_vol._128 | General Assembly]] of Hackuarium association. Followed by a fondue! All members welcome to talk about 2017!<br />
:<li>'''2016.11.28''' Thursday 19:30-20:30: [[20161128_Board_meeting | Board meeting]]<br />
:<li>'''2016.11.02''' Wednesday 19:30-22:00: [[Openhackuarium_vol._122 | Openhackuarium vol. 122]] Epidemium and Echopen: when Open Source meets Pharma and Medtech<br />
:<li>'''2016.10.24''' Monday 19:30-20:30: [[20161024_Board_meeting | Board meeting]]<br />
:<li>'''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]] "Dessine moi un UC"<br />
:<li>'''2016.10.02''' Sunday 10:00-18:00: [[OP_Greenhouse | OP Greenhouse]] building the first P1 iteration<br />
:<li>'''2016.10.01''' Saturday 11:30-18:30: [[Balloon_BBQ | Balloon Launch and BBQ]] Octanis will launch their second stratospheric balloon, so why not celebrate?<br />
<br />
==Other Upcoming Events at [http://www.univercite.ch UniverCité]==<br />
<br><br />
<br />
:<li>'''2016.11.25-27''' Friday-Sunday 9:30-18:30: [[Science_Hackathon_#SHUC2016 | Science Hackathon 2016]] The first ever Science Hackathon at UniverCité: let's hack research!<br />
<br />
==Other Upcoming Events==<br />
<br><br />
<br />
:<li>'''2016.09.25-26''' Sunday-Monday [https://2016.aalhackathon.eu Hack for Ageing Well] is part of the [http://www.aalforum.eu AAL Forum] taking place in St. Gallen<br />
:<li>'''2016.11.19-29''' Saturday-Sunday [http://lauzhack.com Lauzhack] at EPFL is organised by CLIC, the Computer & Communication Sciences students' association<br />
<br />
==Past Hackuarium Events==<br />
<br><br />
You can find the complete list of our '''past events''' [[past events | here]].<br />
<br />
<br><br />
<br />
=Projects=<br />
<br />
Here, you will find the projects going on in the Hackuarium.<br />
<br />
== Active Projects ==<br />
<br />
* [[Hacktion | Hacktion challenges]]<br />
* [[Pushing_the_P1 | The P1 project]]<br />
* [[BeerDeCoded]]<br />
* [[Living Instruments]]<br />
* [[Protein purification systems]]<br />
* [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Micro to Macro Water Pollution]] - with [http://www.hammerdirt.ch/ Hammerdirt] and [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Spectro-pointer]]<br />
* [[Darty_Monkeys]]<br />
* [[Octanis]] - with more information about their Rover / Balloon [http://octanis.org/rover here]<br />
* [[Grow%26Mix_Bioink]]<br />
* [[unmapping]]<br />
* [[AGiR! for genomic integrity]]<br />
* [[Open_Source_Bioreactor | Open Source Bioreactor]]<br />
* [[Bioreactor | Steady state bioreactor]]<br />
<br />
== Archived Projects ==<br />
<br />
* [[Open-Food-DNA |Open-Food-DNA]]<br />
* [[Bience | Bience (Beer and Science!)]]<br />
* [[Mycelium Structures]]<br />
* [[Live Type]]<br />
* [[Projects:LivingLab Design|LivingLab Design]]<br />
* [[Projects:bioluminescence|Bioluminescence]]<br />
* [[Projects:Quantitative Anthropology|Quantitative Anthropology]]<br />
* [[Projects:Impression 3D|Impression 3D]]<br />
* [[Projects:Dépollution des stands de tirs Suisses]]<br />
* [[Incubateur bon marché]]<br />
* [[Space Seed]]<br />
* [[Reception d'images satellites]]<br />
* [[Edible wall]]<br />
* [[ZéBU CH]]<br />
* [[Compost Bacteria]]<br />
<br />
<br><br><br />
<br />
= Practical Informations =<br />
<br />
Following this [[ Media:Univercite map2-01.png | link]] you will find a general plan of Hackuarium.<br><br />
Following this [[ Media:Map 2-01.png | link]], you will find the plan of Hackuarium with the position of equipment.<br />
<br />
== [[Life_Main | Rules at Hackuarium]] ==<br />
[[Life_Main | This page ]] describe the code of conduct for Hackuarium members.<br />
<br />
== [[Protocols | Protocols]] ==<br />
All the protocols that we use at Hackuarium can be found [[ BioP_Main | here ]]<br />
They are detailed and structured so that the main focus is on applications. The idea is to give crucial information on how to apply the most common techniques of the Biology toolbox. The protocols are structured as follow:<br />
::<li> A scheme describing each <br />
::<li> A brief description of the protocol and its use<br />
::<li> A description of the different ways of carrying out the protocol. Ideally there should be a description of a commercial kit and a DIY kit.<br />
::<li> A description of the safety issues and best practice<br />
::<li> Information specific to Hackuarium and where to find the components, tools and reagents.<br />
<br />
== [[Instru_Main | Instruments available]] ==<br />
[[Instru_Main | This page ]] describes all the instruments available at Hackuarium (work in progress..), their location and how to use them.<br />
<br />
== Up-to-date list of instruments we still need ==<br />
This [https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing on-line document] contains all the instruments, tools and consumables we are looking for. Our standard donation contract (French) [[Contrat_de_donation]]. If your organisation happen to discard a piece of equipment we are (or may be) looking for, [mailto:hello@hackuarium.ch contact us]!<br />
<br />
== [[Where we shop | Where we shop]] ==<br />
===Wetware===<br />
Consumables can be purchased from [http://www.huberlab.ch Huber Lab].<br />
<br />
Swiss-based [http://www.smiples.ch Smiples] has a very extensive listing of second hand laboratory and technical items. These can be bought from their online shop or from [https://www.ricardo.ch/online-shop/smiples/?SellerNickName=Smiples Ricardo].<br />
<br />
===Hardware===<br />
Options for electronic parts<br />
* [http://www.ebay.com Ebay] (international)<br />
* [http://www.aliexpress.com AliExpress] (China)<br />
* [https://www.adafruit.com Adafruit] (UK)<br />
* [http://www.seeedstudio.com/depot/ Seeedstudio] (China)<br />
* [http://www.tmart.com/ Tmart] (Hong Kong)<br />
* [https://www.pi-shop.ch/ Rasberry pi shop] (Switzerland)<br />
* [http://www.distrelec.ch/ Distrilec] (Switzerland)<br />
<br />
==How to built a new project page on our Wiki==<br />
<br />
If you want to start or edit a project page on Hackuarium's wiki, please follow these [[Editing the wiki | instructions]].<br />
<br><br />
<br />
==Library and ReadingList ==<br />
<br />
=== The DIY biology / Biohacking movement ===<br />
<li>[http://delfanti.org/biohackers/ Biohackers. The politics of open science] by Alessandro Delfanti (2013) London: Pluto Press.<br />
::[http://delfanti.org/wp-content/uploads/2013/05/Science-2014-Golinelli-521.pdf A review] by [[User:luchenry | Luc Henry]]<br />
<li>[http://link.springer.com/article/10.1186/s40504-016-0039-1 DIY-Bio – economic, epistemological and ethical implications and ambivalences] by Jozef Keulartz and Henk van den Belt (2016) Life Sciences, Society and Policy<br />
<li>[http://www.biobasedworldnews.com/biohacking-everything-you-need-to-know-about-diy-biology Biohacking] everything you need to know about diy biology, (Accessed 05.2016)<br><br />
<li>[http://www.prnewswire.com/news-releases/genspace-nyc-receives-350000-in-support-from-the-simons-foundation-300237457.html Genspace gets funding], PRNewswire, (Accessed 05.2016)<br><br />
<li>[http://www.nature.com/news/governance-learn-from-diy-biologists-1.19507 Learn from DIY biologists], Todd Kuiken, Nature, (Accessed 05.2016)<br><br />
<br />
=== Open Hardware ===<br />
<li>[http://www.nature.com/news/open-hardware-pioneers-push-for-low-cost-lab-kit-1.19518 Open-hardware, pioneers push for low-cost lab kit]Elizabeth Gibney, Nature, (Accessed 05.2016)<br><br />
<li>[http://www.appropedia.org/Open-source_Lab#Examples Appropedia] a source of DIY instruments builds, Dr. Joshua Pearce, (Accessed 05.2016)<br><br />
<li>[http://www.ohwr.org/projects/fr_pcb_mm The PCB milling machine], Open Hardware Repository, (Accessed 05.2016)<br><br />
:: [https://pt.wikiversity.org/wiki/Pesquisa:Ferramentas_livres:Work_group_for_development_of_the_hyperobject_workbench More]<br />
<li>http://www.creativeapplications.net/processing/sensortape-3d-aware-dense-sensor-network-on-a-tape/, Sensory network on a roll of tape, Filip Visnjic, (Accessed 05.2016)<br><br />
<li>[http://oceanoptics.com/product/redeye-oxygen-sensing-patches/ RedEye Oxygen Sensing Patches], Ocean Optics, (Accessed 05.2016) <br><br />
<br><br />
<br />
=== Art ===<br />
<li> [http://www.booooooom.com/2016/04/04/mattia-menchetti-gives-wasps-coloured-paper-to-create-rainbow-nests/#more-78906 Rainbow wasp nests], Mattia Menchetti, (Accessed 05.2016)<br><br />
<li> [http://thecreatorsproject.vice.com/blog/dirty-beats-hear-music-generated-from-bacteria Dirty beats] making music with bacteria, Interspecifics(Accessed 05.2016)<br><br />
::[http://www.interspecifics.cc/-/ More1], [http://www.ncbi.nlm.nih.gov/pubmed/21239171, More2], [http://www.ncbi.nlm.nih.gov/pubmed/12501293, More3]<br><br />
<br><br />
<br />
=== Ideas/Concepts/Science ===<br />
<li>[http://ideas.ted.com/a-newly-drawn-tree-of-life-reminds-us-to-question-what-we-know/ The new tree of life] TED (Accessed 05.2016)<br><br />
<li>[https://medium.com/now-of-work/the-workplace-of-the-future-work-of-today-16c58df1fa29#.zhbu0c57f Rethinking work], Yann Heurtaux, (Accessed 05.2016)<br><br />
<li>[https://www.kickstarter.com/projects/910418035/plastic-bottle-cutter That plastic bottle cutter], Pavel & Ian, (Accessed 05.2016)<br><br />
<br><br />
<br />
=== Open Source ===<br />
<li>[https://blogs.openaire.eu/?p=882 Results held hostage], Francesco Mondada, (Accessed 05.2016)<br><br />
<br><br />
<br />
== Media and Press ==<br />
<br />
* 2016.09.27 - Hackuarium is mentioned in a paper by Celia Luterbacher on citizen science: [http://www.swissinfo.ch/eng/democratisation_citizen-science--not-a-scientist--not-a-problem/42437940 Citizen science: Not a scientist? Not a problem]<br />
<br />
*2016.09.18 - [[User:Luchenry | Luc Henry]], co-founder of Hackuarium, is interviewed in this podcast on citizen science: [http://www.swissinfo.ch/eng/podcast_science-for-non-scientists/42438132 Science for non-scientists]<br />
<br />
*<br />
<br />
== Wanted ==<br />
Do you want to contribute to making Hackuarium a diverse and lively community? Check out our wanted lists:<br />
::<li>Skills<br />
::<li>Ideas<br />
::<li>[https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing Equipment]<br />
::<li>Consumables<br />
<br />
<br><br></div>Randogphttps://wiki.hackuarium.ch/index.php?title=20160919_Board_meeting&diff=791520160919 Board meeting2016-09-19T09:47:44Z<p>Randogp: /* Hackuarium in general */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Thursday, September 19th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* Sam<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0.1: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 0.2: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee using [http://www.smallinvoice.ch/CH/en/home/Create-and-manage-invoices-and-offers-for-free.html Smallinvoice.ch] -> Luc (up to end July, then for 3 months) -> Luc<br />
<br><br />
-> make a slide to insist at OH that people should talk about the place and advertise for it and become members (and how) -> Luc<br />
<br><br />
<br />
To board members: <br><br />
-> Email board member and ask for dedication (2 hours a week + list of tasks) -> Luc to present at this board meeting <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Implement [https://en.wikipedia.org/wiki/Performance_indicator key performance indicators] until the next General Assembly on Wednesday 14th December 2016 (Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) <br><br />
-> Send a summary with membership fees and conditions <br><br />
-> Profile for 2 interns, including tasks and skills <br><br />
-> List of projects that need help, list of tasks <br><br />
-> Plan GA OH #128 Wed. 14th December <br><br />
-> Next board meetings 19th of Sept. 24th October, 28th November -> Luc sent a reminder on 31st August to board members<br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue). <br><br />
<br />
Luc asks Marc about the projector -> email sent on 31.08.2016<br><br />
Luc clarifies the role of Juliette and how we can help her help us <br><br />
Anne-Laure makes a list of people to invite in 2017 <br><br />
Every board member sends an email/call to 2-3 active projects and check where they are and report at the next board meeting, what is stopping you, what would you need to come more often <br><br />
* Gianpaolo --> Rachel: I need distilled water, the brewery is not using it and the pipe is not working.<br />
Luc asks Cyanoguard what he expects from the lab. <br><br />
Luc asks Luc about bioreactor documentation -> done, Luc still needs to insist to make sure it is now completed properly<br><br />
Sam asks Marion about Air Sampler on OH. <br><br />
Make sure we have a list of tasks to get people on board -> post-its with projects and tasks so that people can sign-up. <br><br />
Sam gets RFID stickers to attach to the main card. <br><br />
Sam makes primary twitter channel and see how many unfollow (1month). <br><br />
Luc sends Pi workshop tweets. Done <br><br />
Yann sends Facebook message with Hackuarium. <br><br />
<br />
==Point 1: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
'''Yann (5min)'''<br />
*Board: Redefine roles: Board members have a task and commit to it, they invest time and energy in a tangible way.<br />
*Association: Maybe I'm just the one losing track but I have the feeling things are not moving or are just going randomly in any direction<br />
::* Do we try to set ourselves a goal as an association and try to push most of the members in this direction ? If we want to make an impact we have to pool our ressources no ? We have some main projects going well since people are independent and work as a team but we also have a lot of free electrons we could catalyse in something bigger ? WDYT ?<br />
'''Gianpaolo (5min)'''<br />
What is Hackuarium today, what is the 1 year and 5 year impact that we want to accomplish?<br />
* The OpenHackuarium<br />
* Hackuarium as DIY-laboratory, coworking-laboratory<br />
* Hackuarium as flagship model of openscience projects<br />
* The board and president of Hackuarium [https://docs.google.com/spreadsheets/d/1jMdJHiBy0dP332PSjo13YyiXNmXy5g5LwgOy5s1k-W8/edit?usp=sharing KPI 2016 presences]<br />
* Hackuarium or UC?<br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Luc (5 min)<br><br />
Update from Yann (1min to 10min)<br><br />
:: Where are we going ?<br />
:: How do we avoid UC becoming just another coworking?<br />
::: Do we want to put energy into this when I (Yann) feel no return whatsoever and mostly a loss of time and energy ?<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
- BSO title obtained<br />
- Wall and renovation problems.<br />
:: When are those renovations due ?<br />
:: What do we do with the material ?<br />
:: Sam was talking about amovible walls<br />
:: Yann: PhD and followings<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
* Web app architecture (Marco). It automagically recreates the graphs as new beers are introduced. http://decodedbeer-twoba.rhcloud.com/polls/beerchart_json/<br />
** The database works well back-end.<br />
** Help needed on Front-end (design). (Vanessa?)<br />
** Help needed on algorithms to mangle data. (Jonathan keen to do some work now that the PhD has been defended)<br />
<br />
*Bench work<br />
** Work done:<br />
** 96 beer pellets (Gianpaolo)<br />
** 96 DNA extractions (Gabrielle)<br />
** 192 PCRs: 96 x 16s bacteria, 96 x ITS fungi (Nicolas), --> sent to genomic facility for sequencing.<br />
** Only 39 PCR (ITS) are OK for sequencing.<br />
** New team member Isabelle, keen to work on the bench from October 1st.<br />
<br />
*QC at Hackuarium<br />
** Probably need for a more clean area to work with DNA (Gustavo, see P1 discussion)<br />
** After a DNA extraction, do we have DNA? Is it pure or contaminated? (Open nanodrop: Sam, Jochem)<br />
** After a PCR, did the PCR worked? Has it the right band size?<br />
<br />
*Bento prototype returned to fabric. Final device expected in November.<br />
<br />
===Living Instruments===<br />
Update from Luc (5min)<br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
==Point 2: Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application will be submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] is October 15th.<br />
<br />
<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br />
<br />
==Point 3: Future partnerships==<br />
<br />
===EPFL - Engineering and Architect internships===<br />
<br />
<br />
===Mike Horn pole2pole===<br />
<br />
<br />
===Joint Outreach with SIB===<br />
<br />
Waiting for Marie-Claude Blatter to come back to us. Reminder email sent on 31.08.2016, answered on 01.09.2016 <br><br />
<br />
She discussed with Nicolas Guex et Marco Pagni (Vital It SIB). A brief summary is available [https://docs.google.com/document/d/1Lwz7kxW3JrjqMhE_uqvNy3y7QEowYo4WVx6jQJ8fzjc/edit?usp=sharing here].<br><br />
<br />
Ideas include RNA seq on samples to see if there are metabolically active species in clouds. A first sampling session would give an idea (on Oct. 1st with baloon launch?). SIB can only bring expertise in data analysis. The sequencing costs and handling would have to be done with another partner (UNIL, Keith Harshman / Fasteris).<br />
<br />
==Point 4: Finance==<br />
<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
Very proud to say we have several events already planned this autumn:<br />
<br />
<br />
Still need to plan the following events:<br />
<br />
===Molecular Biology day/evening===<br />
<br />
Luc (3 min)<br />
<br />
Was moved to mid-october so we can advertise for it at the [[Openhackuarium_vol._116 | Openhackuarium vol. 116]].<br />
<br />
*WHEN? '''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]]<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
===Machine learning for image recognition===<br />
<br />
*WHEN?<br />
*WHO? Yann P. + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? A friend of Yann P. wants to present and bring a concrete case where we can show how with a RasPi we can differentiate things. A useful tech if for ex we want to look at wastes sorting.<br />
<br />
==Point 5bis: Other Events==<br />
<br />
<br />
==Point 6: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
===presence tracker===<br />
Are we happy with that?<br />
<br />
===meetup===<br />
Are we happy with that?<br />
<br />
==Point 7: Hacktion==<br />
<br />
<br />
==Point 8: Replication==<br />
(Luc, 5 min)<br />
3 projects in 2017<br />
<br />
How far did we go? <br />
<br />
Open Nanodrop:<br />
* Octanis hacked the Ocean Optics Spectrometer (200-1100nm) already<br />
* UV light source (Yann?) and SMA905 UV fibre optic cables ([http://www.ebay.com/itm/SMA905-fiber-optic-connector-UV-ultraviolet-visible-laser-fiber-core-200um-/232004914961?hash=item3604920f11:g:xGMAAOSwkl5XfaK8 50usd ebay]) missing<br />
::* Yann: We can use the leds for sure. I still got several of them! I don't think we need optical fibers because we can use UV detectors. <br />
* Good for DNA QC in future P1 (-> 230nm, 260nm, 280nm)<br />
::* Yep of course it is necessary for quantification! We could have gone low-cost and rely on the gel intensity.<br />
<br />
[[File:Screen Shot 2016-08-18 at 15.55.26.png]]<br />
<br />
==Point 9: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20160919_Board_meeting&diff=791420160919 Board meeting2016-09-19T09:45:49Z<p>Randogp: /* Hackuarium in general */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Thursday, September 19th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* Sam<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0.1: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 0.2: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee using [http://www.smallinvoice.ch/CH/en/home/Create-and-manage-invoices-and-offers-for-free.html Smallinvoice.ch] -> Luc (up to end July, then for 3 months) -> Luc<br />
<br><br />
-> make a slide to insist at OH that people should talk about the place and advertise for it and become members (and how) -> Luc<br />
<br><br />
<br />
To board members: <br><br />
-> Email board member and ask for dedication (2 hours a week + list of tasks) -> Luc to present at this board meeting <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Implement [https://en.wikipedia.org/wiki/Performance_indicator key performance indicators] until the next General Assembly on Wednesday 14th December 2016 (Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) <br><br />
-> Send a summary with membership fees and conditions <br><br />
-> Profile for 2 interns, including tasks and skills <br><br />
-> List of projects that need help, list of tasks <br><br />
-> Plan GA OH #128 Wed. 14th December <br><br />
-> Next board meetings 19th of Sept. 24th October, 28th November -> Luc sent a reminder on 31st August to board members<br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue). <br><br />
<br />
Luc asks Marc about the projector -> email sent on 31.08.2016<br><br />
Luc clarifies the role of Juliette and how we can help her help us <br><br />
Anne-Laure makes a list of people to invite in 2017 <br><br />
Every board member sends an email/call to 2-3 active projects and check where they are and report at the next board meeting, what is stopping you, what would you need to come more often <br><br />
* Gianpaolo --> Rachel: I need distilled water, the brewery is not using it and the pipe is not working.<br />
Luc asks Cyanoguard what he expects from the lab. <br><br />
Luc asks Luc about bioreactor documentation -> done, Luc still needs to insist to make sure it is now completed properly<br><br />
Sam asks Marion about Air Sampler on OH. <br><br />
Make sure we have a list of tasks to get people on board -> post-its with projects and tasks so that people can sign-up. <br><br />
Sam gets RFID stickers to attach to the main card. <br><br />
Sam makes primary twitter channel and see how many unfollow (1month). <br><br />
Luc sends Pi workshop tweets. Done <br><br />
Yann sends Facebook message with Hackuarium. <br><br />
<br />
==Point 1: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
'''Yann (5min)'''<br />
*Board: Redefine roles: Board members have a task and commit to it, they invest time and energy in a tangible way.<br />
*Association: Maybe I'm just the one losing track but I have the feeling things are not moving or are just going randomly in any direction<br />
::* Do we try to set ourselves a goal as an association and try to push most of the members in this direction ? If we want to make an impact we have to pool our ressources no ? We have some main projects going well since people are independent and work as a team but we also have a lot of free electrons we could catalyse in something bigger ? WDYT ?<br />
'''Gianpaolo (5min)'''<br />
What is Hackuarium today, what is the 1 year and 5 year impact that we want to accomplish?<br />
* The OpenHackuarium<br />
* Hackuarium as laboratory<br />
* Hackuarium as community<br />
* The board and president of Hackuarium [https://docs.google.com/spreadsheets/d/1jMdJHiBy0dP332PSjo13YyiXNmXy5g5LwgOy5s1k-W8/edit?usp=sharing KPI 2016 presences]<br />
* Hackuarium or UC?<br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Luc (5 min)<br><br />
Update from Yann (1min to 10min)<br><br />
:: Where are we going ?<br />
:: How do we avoid UC becoming just another coworking?<br />
::: Do we want to put energy into this when I (Yann) feel no return whatsoever and mostly a loss of time and energy ?<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
- BSO title obtained<br />
- Wall and renovation problems.<br />
:: When are those renovations due ?<br />
:: What do we do with the material ?<br />
:: Sam was talking about amovible walls<br />
:: Yann: PhD and followings<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
* Web app architecture (Marco). It automagically recreates the graphs as new beers are introduced. http://decodedbeer-twoba.rhcloud.com/polls/beerchart_json/<br />
** The database works well back-end.<br />
** Help needed on Front-end (design). (Vanessa?)<br />
** Help needed on algorithms to mangle data. (Jonathan keen to do some work now that the PhD has been defended)<br />
<br />
*Bench work<br />
** Work done:<br />
** 96 beer pellets (Gianpaolo)<br />
** 96 DNA extractions (Gabrielle)<br />
** 192 PCRs: 96 x 16s bacteria, 96 x ITS fungi (Nicolas), --> sent to genomic facility for sequencing.<br />
** Only 39 PCR (ITS) are OK for sequencing.<br />
** New team member Isabelle, keen to work on the bench from October 1st.<br />
<br />
*QC at Hackuarium<br />
** Probably need for a more clean area to work with DNA (Gustavo, see P1 discussion)<br />
** After a DNA extraction, do we have DNA? Is it pure or contaminated? (Open nanodrop: Sam, Jochem)<br />
** After a PCR, did the PCR worked? Has it the right band size?<br />
<br />
*Bento prototype returned to fabric. Final device expected in November.<br />
<br />
===Living Instruments===<br />
Update from Luc (5min)<br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
==Point 2: Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application will be submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] is October 15th.<br />
<br />
<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br />
<br />
==Point 3: Future partnerships==<br />
<br />
===EPFL - Engineering and Architect internships===<br />
<br />
<br />
===Mike Horn pole2pole===<br />
<br />
<br />
===Joint Outreach with SIB===<br />
<br />
Waiting for Marie-Claude Blatter to come back to us. Reminder email sent on 31.08.2016, answered on 01.09.2016 <br><br />
<br />
She discussed with Nicolas Guex et Marco Pagni (Vital It SIB). A brief summary is available [https://docs.google.com/document/d/1Lwz7kxW3JrjqMhE_uqvNy3y7QEowYo4WVx6jQJ8fzjc/edit?usp=sharing here].<br><br />
<br />
Ideas include RNA seq on samples to see if there are metabolically active species in clouds. A first sampling session would give an idea (on Oct. 1st with baloon launch?). SIB can only bring expertise in data analysis. The sequencing costs and handling would have to be done with another partner (UNIL, Keith Harshman / Fasteris).<br />
<br />
==Point 4: Finance==<br />
<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
Very proud to say we have several events already planned this autumn:<br />
<br />
<br />
Still need to plan the following events:<br />
<br />
===Molecular Biology day/evening===<br />
<br />
Luc (3 min)<br />
<br />
Was moved to mid-october so we can advertise for it at the [[Openhackuarium_vol._116 | Openhackuarium vol. 116]].<br />
<br />
*WHEN? '''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]]<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
===Machine learning for image recognition===<br />
<br />
*WHEN?<br />
*WHO? Yann P. + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? A friend of Yann P. wants to present and bring a concrete case where we can show how with a RasPi we can differentiate things. A useful tech if for ex we want to look at wastes sorting.<br />
<br />
==Point 5bis: Other Events==<br />
<br />
<br />
==Point 6: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
===presence tracker===<br />
Are we happy with that?<br />
<br />
===meetup===<br />
Are we happy with that?<br />
<br />
==Point 7: Hacktion==<br />
<br />
<br />
==Point 8: Replication==<br />
(Luc, 5 min)<br />
3 projects in 2017<br />
<br />
How far did we go? <br />
<br />
Open Nanodrop:<br />
* Octanis hacked the Ocean Optics Spectrometer (200-1100nm) already<br />
* UV light source (Yann?) and SMA905 UV fibre optic cables ([http://www.ebay.com/itm/SMA905-fiber-optic-connector-UV-ultraviolet-visible-laser-fiber-core-200um-/232004914961?hash=item3604920f11:g:xGMAAOSwkl5XfaK8 50usd ebay]) missing<br />
::* Yann: We can use the leds for sure. I still got several of them! I don't think we need optical fibers because we can use UV detectors. <br />
* Good for DNA QC in future P1 (-> 230nm, 260nm, 280nm)<br />
::* Yep of course it is necessary for quantification! We could have gone low-cost and rely on the gel intensity.<br />
<br />
[[File:Screen Shot 2016-08-18 at 15.55.26.png]]<br />
<br />
==Point 9: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=KPI&diff=7913KPI2016-09-19T09:18:58Z<p>Randogp: </p>
<hr />
<div>[https://en.wikipedia.org/wiki/Performance_indicator Key performance indicators] help "evaluate the success of an organization or of a particular activity in which it engages."<br />
<br />
To measure how the Hackuarium community evolves, Gianpaolo & Sam are monitoring the following indicators:<br />
<br />
* # members<br />
* # slack messages<br />
* # active projects (min. to be defined)<br />
* # internal events<br />
* # external events<br />
* # media mentions<br />
* FB likes<br />
* Twitter followers<br />
* Checkins<br />
* # OH visitors (RSVPs: show/noshow ratio)<br />
* Board members attending the board meetings https://docs.google.com/spreadsheets/d/1jMdJHiBy0dP332PSjo13YyiXNmXy5g5LwgOy5s1k-W8/edit?usp=sharing.<br />
<br />
#OpenHackuarium 113: 40 people on 31.08.2016<br />
#OpenHackuarium 114: 31 people on 07.09.2016<br />
#OpenHackuarium 115: 32 people on 14.09.2016</div>Randogphttps://wiki.hackuarium.ch/index.php?title=KPI&diff=7912KPI2016-09-19T09:00:49Z<p>Randogp: </p>
<hr />
<div>[https://en.wikipedia.org/wiki/Performance_indicator Key performance indicators] help "evaluate the success of an organization or of a particular activity in which it engages."<br />
<br />
To measure how the Hackuarium community evolves, Gianpaolo & Sam are monitoring the following indicators:<br />
<br />
* # members<br />
* # slack messages<br />
* # active projects (min. to be defined)<br />
* # internal events<br />
* # external events<br />
* # media mentions<br />
* FB likes<br />
* Twitter followers<br />
* Checkins<br />
* # OH visitors (RSVPs: show/noshow ratio)<br />
* Board meeting participants.<br />
<br />
#OpenHackuarium 113: 40 people on 31.08.2016<br />
#OpenHackuarium 114: 31 people on 07.09.2016<br />
#OpenHackuarium 115: 32 people on 14.09.2016</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20160919_Board_meeting&diff=791120160919 Board meeting2016-09-19T08:59:45Z<p>Randogp: /* Point 0.2: Actionables */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Thursday, September 19th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* Sam<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0.1: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 0.2: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee using [http://www.smallinvoice.ch/CH/en/home/Create-and-manage-invoices-and-offers-for-free.html Smallinvoice.ch] -> Luc (up to end July, then for 3 months) -> Luc<br />
<br><br />
-> make a slide to insist at OH that people should talk about the place and advertise for it and become members (and how) -> Luc<br />
<br><br />
<br />
To board members: <br><br />
-> Email board member and ask for dedication (2 hours a week + list of tasks) -> Luc to present at this board meeting <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Implement [https://en.wikipedia.org/wiki/Performance_indicator key performance indicators] until the next General Assembly on Wednesday 14th December 2016 (Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) <br><br />
-> Send a summary with membership fees and conditions <br><br />
-> Profile for 2 interns, including tasks and skills <br><br />
-> List of projects that need help, list of tasks <br><br />
-> Plan GA OH #128 Wed. 14th December <br><br />
-> Next board meetings 19th of Sept. 24th October, 28th November -> Luc sent a reminder on 31st August to board members<br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue). <br><br />
<br />
Luc asks Marc about the projector -> email sent on 31.08.2016<br><br />
Luc clarifies the role of Juliette and how we can help her help us <br><br />
Anne-Laure makes a list of people to invite in 2017 <br><br />
Every board member sends an email/call to 2-3 active projects and check where they are and report at the next board meeting, what is stopping you, what would you need to come more often <br><br />
* Gianpaolo --> Rachel: I need distilled water, the brewery is not using it and the pipe is not working.<br />
Luc asks Cyanoguard what he expects from the lab. <br><br />
Luc asks Luc about bioreactor documentation -> done, Luc still needs to insist to make sure it is now completed properly<br><br />
Sam asks Marion about Air Sampler on OH. <br><br />
Make sure we have a list of tasks to get people on board -> post-its with projects and tasks so that people can sign-up. <br><br />
Sam gets RFID stickers to attach to the main card. <br><br />
Sam makes primary twitter channel and see how many unfollow (1month). <br><br />
Luc sends Pi workshop tweets. Done <br><br />
Yann sends Facebook message with Hackuarium. <br><br />
<br />
==Point 1: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
'''Yann (5min)'''<br />
*Board: Redefine roles: Board members have a task and commit to it, they invest time and energy in a tangible way.<br />
*Association: Maybe I'm just the one losing track but I have the feeling things are not moving or are just going randomly in any direction<br />
::* Do we try to set ourselves a goal as an association and try to push most of the members in this direction ? If we want to make an impact we have to pool our ressources no ? We have some main projects going well since people are independent and work as a team but we also have a lot of free electrons we could catalyse in something bigger ? WDYT ?<br />
'''Gianpaolo (5min)'''<br />
What is Hackuarium today, what is the 1 year and 5 year impact that we want to accomplish?<br />
* The OpenHackuarium<br />
* Hackuarium as laboratory<br />
* Hackuarium as community<br />
* The board and president of Hackuarium<br />
* Hackuarium or UC?<br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Luc (5 min)<br><br />
Update from Yann (1min to 10min)<br><br />
:: Where are we going ?<br />
:: How do we avoid UC becoming just another coworking?<br />
::: Do we want to put energy into this when I (Yann) feel no return whatsoever and mostly a loss of time and energy ?<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
- BSO title obtained<br />
- Wall and renovation problems.<br />
:: When are those renovations due ?<br />
:: What do we do with the material ?<br />
:: Sam was talking about amovible walls<br />
:: Yann: PhD and followings<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
* Web app architecture (Marco). It automagically recreates the graphs as new beers are introduced. http://decodedbeer-twoba.rhcloud.com/polls/beerchart_json/<br />
** The database works well back-end.<br />
** Help needed on Front-end (design). (Vanessa?)<br />
** Help needed on algorithms to mangle data. (Jonathan keen to do some work now that the PhD has been defended)<br />
<br />
*Bench work<br />
** Work done:<br />
** 96 beer pellets (Gianpaolo)<br />
** 96 DNA extractions (Gabrielle)<br />
** 192 PCRs: 96 x 16s bacteria, 96 x ITS fungi (Nicolas), --> sent to genomic facility for sequencing.<br />
** Only 39 PCR (ITS) are OK for sequencing.<br />
** New team member Isabelle, keen to work on the bench from October 1st.<br />
<br />
*QC at Hackuarium<br />
** Probably need for a more clean area to work with DNA (Gustavo, see P1 discussion)<br />
** After a DNA extraction, do we have DNA? Is it pure or contaminated? (Open nanodrop: Sam, Jochem)<br />
** After a PCR, did the PCR worked? Has it the right band size?<br />
<br />
*Bento prototype returned to fabric. Final device expected in November.<br />
<br />
===Living Instruments===<br />
Update from Luc (5min)<br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
==Point 2: Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application will be submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] is October 15th.<br />
<br />
<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br />
<br />
==Point 3: Future partnerships==<br />
<br />
===EPFL - Engineering and Architect internships===<br />
<br />
<br />
===Mike Horn pole2pole===<br />
<br />
<br />
===Joint Outreach with SIB===<br />
<br />
Waiting for Marie-Claude Blatter to come back to us. Reminder email sent on 31.08.2016, answered on 01.09.2016 <br><br />
<br />
She discussed with Nicolas Guex et Marco Pagni (Vital It SIB). A brief summary is available [https://docs.google.com/document/d/1Lwz7kxW3JrjqMhE_uqvNy3y7QEowYo4WVx6jQJ8fzjc/edit?usp=sharing here].<br><br />
<br />
Ideas include RNA seq on samples to see if there are metabolically active species in clouds. A first sampling session would give an idea (on Oct. 1st with baloon launch?). SIB can only bring expertise in data analysis. The sequencing costs and handling would have to be done with another partner (UNIL, Keith Harshman / Fasteris).<br />
<br />
==Point 4: Finance==<br />
<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
Very proud to say we have several events already planned this autumn:<br />
<br />
<br />
Still need to plan the following events:<br />
<br />
===Molecular Biology day/evening===<br />
<br />
Luc (3 min)<br />
<br />
Was moved to mid-october so we can advertise for it at the [[Openhackuarium_vol._116 | Openhackuarium vol. 116]].<br />
<br />
*WHEN? '''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]]<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
===Machine learning for image recognition===<br />
<br />
*WHEN?<br />
*WHO? Yann P. + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? A friend of Yann P. wants to present and bring a concrete case where we can show how with a RasPi we can differentiate things. A useful tech if for ex we want to look at wastes sorting.<br />
<br />
==Point 5bis: Other Events==<br />
<br />
<br />
==Point 6: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
===presence tracker===<br />
Are we happy with that?<br />
<br />
===meetup===<br />
Are we happy with that?<br />
<br />
==Point 7: Hacktion==<br />
<br />
<br />
==Point 8: Replication==<br />
(Luc, 5 min)<br />
3 projects in 2017<br />
<br />
How far did we go? <br />
<br />
Open Nanodrop:<br />
* Octanis hacked the Ocean Optics Spectrometer (200-1100nm) already<br />
* UV light source (Yann?) and SMA905 UV fibre optic cables ([http://www.ebay.com/itm/SMA905-fiber-optic-connector-UV-ultraviolet-visible-laser-fiber-core-200um-/232004914961?hash=item3604920f11:g:xGMAAOSwkl5XfaK8 50usd ebay]) missing<br />
::* Yann: We can use the leds for sure. I still got several of them! I don't think we need optical fibers because we can use UV detectors. <br />
* Good for DNA QC in future P1 (-> 230nm, 260nm, 280nm)<br />
::* Yep of course it is necessary for quantification! We could have gone low-cost and rely on the gel intensity.<br />
<br />
[[File:Screen Shot 2016-08-18 at 15.55.26.png]]<br />
<br />
==Point 9: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20160919_Board_meeting&diff=791020160919 Board meeting2016-09-19T08:54:46Z<p>Randogp: /* Hackuarium in general */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Thursday, September 19th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* Sam<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0.1: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 0.2: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee using [http://www.smallinvoice.ch/CH/en/home/Create-and-manage-invoices-and-offers-for-free.html Smallinvoice.ch] -> Luc (up to end July, then for 3 months) -> Luc<br />
<br><br />
-> make a slide to insist at OH that people should talk about the place and advertise for it and become members (and how) -> Luc<br />
<br><br />
<br />
To board members: <br><br />
-> Email board member and ask for dedication (2 hours a week + list of tasks) -> Luc to present at this board meeting <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Implement [https://en.wikipedia.org/wiki/Performance_indicator key performance indicators] until the next General Assembly on Wednesday 14th December 2016 (Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) <br><br />
-> Send a summary with membership fees and conditions <br><br />
-> Profile for 2 interns, including tasks and skills <br><br />
-> List of projects that need help, list of tasks <br><br />
-> Plan GA OH #128 Wed. 14th December <br><br />
-> Next board meetings 19th of Sept. 24th October, 28th November -> Luc sent a reminder on 31st August to board members<br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue). <br><br />
<br />
Luc asks Marc about the projector -> email sent on 31.08.2016<br><br />
Luc clarifies the role of Juliette and how we can help her help us <br><br />
Anne-Laure makes a list of people to invite in 2017 <br><br />
Every board member sends an email/call to 2-3 active projects and check where they are and report at the next board meeting, what is stopping you, what would you need to come more often <br><br />
Luc asks Cyanoguard what he expects from the lab. <br><br />
Luc asks Luc about bioreactor documentation -> done, Luc still needs to insist to make sure it is now completed properly<br><br />
Sam asks Marion about Air Sampler on OH. <br><br />
Make sure we have a list of tasks to get people on board -> post-its with projects and tasks so that people can sign-up. <br><br />
Sam gets RFID stickers to attach to the main card. <br><br />
Sam makes primary twitter channel and see how many unfollow (1month). <br><br />
Luc sends Pi workshop tweets. Done <br><br />
Yann sends Facebook message with Hackuarium. <br><br />
<br />
==Point 1: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
'''Yann (5min)'''<br />
*Board: Redefine roles: Board members have a task and commit to it, they invest time and energy in a tangible way.<br />
*Association: Maybe I'm just the one losing track but I have the feeling things are not moving or are just going randomly in any direction<br />
::* Do we try to set ourselves a goal as an association and try to push most of the members in this direction ? If we want to make an impact we have to pool our ressources no ? We have some main projects going well since people are independent and work as a team but we also have a lot of free electrons we could catalyse in something bigger ? WDYT ?<br />
'''Gianpaolo (5min)'''<br />
What is Hackuarium today, what is the 1 year and 5 year impact that we want to accomplish?<br />
* The OpenHackuarium<br />
* Hackuarium as laboratory<br />
* Hackuarium as community<br />
* The board and president of Hackuarium<br />
* Hackuarium or UC?<br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Luc (5 min)<br><br />
Update from Yann (1min to 10min)<br><br />
:: Where are we going ?<br />
:: How do we avoid UC becoming just another coworking?<br />
::: Do we want to put energy into this when I (Yann) feel no return whatsoever and mostly a loss of time and energy ?<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
- BSO title obtained<br />
- Wall and renovation problems.<br />
:: When are those renovations due ?<br />
:: What do we do with the material ?<br />
:: Sam was talking about amovible walls<br />
:: Yann: PhD and followings<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
* Web app architecture (Marco). It automagically recreates the graphs as new beers are introduced. http://decodedbeer-twoba.rhcloud.com/polls/beerchart_json/<br />
** The database works well back-end.<br />
** Help needed on Front-end (design). (Vanessa?)<br />
** Help needed on algorithms to mangle data. (Jonathan keen to do some work now that the PhD has been defended)<br />
<br />
*Bench work<br />
** Work done:<br />
** 96 beer pellets (Gianpaolo)<br />
** 96 DNA extractions (Gabrielle)<br />
** 192 PCRs: 96 x 16s bacteria, 96 x ITS fungi (Nicolas), --> sent to genomic facility for sequencing.<br />
** Only 39 PCR (ITS) are OK for sequencing.<br />
** New team member Isabelle, keen to work on the bench from October 1st.<br />
<br />
*QC at Hackuarium<br />
** Probably need for a more clean area to work with DNA (Gustavo, see P1 discussion)<br />
** After a DNA extraction, do we have DNA? Is it pure or contaminated? (Open nanodrop: Sam, Jochem)<br />
** After a PCR, did the PCR worked? Has it the right band size?<br />
<br />
*Bento prototype returned to fabric. Final device expected in November.<br />
<br />
===Living Instruments===<br />
Update from Luc (5min)<br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
==Point 2: Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application will be submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] is October 15th.<br />
<br />
<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br />
<br />
==Point 3: Future partnerships==<br />
<br />
===EPFL - Engineering and Architect internships===<br />
<br />
<br />
===Mike Horn pole2pole===<br />
<br />
<br />
===Joint Outreach with SIB===<br />
<br />
Waiting for Marie-Claude Blatter to come back to us. Reminder email sent on 31.08.2016, answered on 01.09.2016 <br><br />
<br />
She discussed with Nicolas Guex et Marco Pagni (Vital It SIB). A brief summary is available [https://docs.google.com/document/d/1Lwz7kxW3JrjqMhE_uqvNy3y7QEowYo4WVx6jQJ8fzjc/edit?usp=sharing here].<br><br />
<br />
Ideas include RNA seq on samples to see if there are metabolically active species in clouds. A first sampling session would give an idea (on Oct. 1st with baloon launch?). SIB can only bring expertise in data analysis. The sequencing costs and handling would have to be done with another partner (UNIL, Keith Harshman / Fasteris).<br />
<br />
==Point 4: Finance==<br />
<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
Very proud to say we have several events already planned this autumn:<br />
<br />
<br />
Still need to plan the following events:<br />
<br />
===Molecular Biology day/evening===<br />
<br />
Luc (3 min)<br />
<br />
Was moved to mid-october so we can advertise for it at the [[Openhackuarium_vol._116 | Openhackuarium vol. 116]].<br />
<br />
*WHEN? '''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]]<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
===Machine learning for image recognition===<br />
<br />
*WHEN?<br />
*WHO? Yann P. + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? A friend of Yann P. wants to present and bring a concrete case where we can show how with a RasPi we can differentiate things. A useful tech if for ex we want to look at wastes sorting.<br />
<br />
==Point 5bis: Other Events==<br />
<br />
<br />
==Point 6: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
===presence tracker===<br />
Are we happy with that?<br />
<br />
===meetup===<br />
Are we happy with that?<br />
<br />
==Point 7: Hacktion==<br />
<br />
<br />
==Point 8: Replication==<br />
(Luc, 5 min)<br />
3 projects in 2017<br />
<br />
How far did we go? <br />
<br />
Open Nanodrop:<br />
* Octanis hacked the Ocean Optics Spectrometer (200-1100nm) already<br />
* UV light source (Yann?) and SMA905 UV fibre optic cables ([http://www.ebay.com/itm/SMA905-fiber-optic-connector-UV-ultraviolet-visible-laser-fiber-core-200um-/232004914961?hash=item3604920f11:g:xGMAAOSwkl5XfaK8 50usd ebay]) missing<br />
::* Yann: We can use the leds for sure. I still got several of them! I don't think we need optical fibers because we can use UV detectors. <br />
* Good for DNA QC in future P1 (-> 230nm, 260nm, 280nm)<br />
::* Yep of course it is necessary for quantification! We could have gone low-cost and rely on the gel intensity.<br />
<br />
[[File:Screen Shot 2016-08-18 at 15.55.26.png]]<br />
<br />
==Point 9: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20160919_Board_meeting&diff=790920160919 Board meeting2016-09-19T08:31:26Z<p>Randogp: /* BeerDeCoded */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Thursday, September 19th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* Sam<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0.1: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 0.2: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee using [http://www.smallinvoice.ch/CH/en/home/Create-and-manage-invoices-and-offers-for-free.html Smallinvoice.ch] -> Luc (up to end July, then for 3 months) -> Luc<br />
<br><br />
-> make a slide to insist at OH that people should talk about the place and advertise for it and become members (and how) -> Luc<br />
<br><br />
<br />
To board members: <br><br />
-> Email board member and ask for dedication (2 hours a week + list of tasks) -> Luc to present at this board meeting <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Implement [https://en.wikipedia.org/wiki/Performance_indicator key performance indicators] until the next General Assembly on Wednesday 14th December 2016 (Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) <br><br />
-> Send a summary with membership fees and conditions <br><br />
-> Profile for 2 interns, including tasks and skills <br><br />
-> List of projects that need help, list of tasks <br><br />
-> Plan GA OH #128 Wed. 14th December <br><br />
-> Next board meetings 19th of Sept. 24th October, 28th November -> Luc sent a reminder on 31st August to board members<br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue). <br><br />
<br />
Luc asks Marc about the projector -> email sent on 31.08.2016<br><br />
Luc clarifies the role of Juliette and how we can help her help us <br><br />
Anne-Laure makes a list of people to invite in 2017 <br><br />
Every board member sends an email/call to 2-3 active projects and check where they are and report at the next board meeting, what is stopping you, what would you need to come more often <br><br />
Luc asks Cyanoguard what he expects from the lab. <br><br />
Luc asks Luc about bioreactor documentation -> done, Luc still needs to insist to make sure it is now completed properly<br><br />
Sam asks Marion about Air Sampler on OH. <br><br />
Make sure we have a list of tasks to get people on board -> post-its with projects and tasks so that people can sign-up. <br><br />
Sam gets RFID stickers to attach to the main card. <br><br />
Sam makes primary twitter channel and see how many unfollow (1month). <br><br />
Luc sends Pi workshop tweets. Done <br><br />
Yann sends Facebook message with Hackuarium. <br><br />
<br />
==Point 1: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
'''Yann (5min)'''<br />
*Board: Redefine roles: Board members have a task and commit to it, they invest time and energy in a tangible way.<br />
*Association: Maybe I'm just the one losing track but I have the feeling things are not moving or are just going randomly in any direction<br />
::* Do we try to set ourselves a goal as an association and try to push most of the members in this direction ? If we want to make an impact we have to pool our ressources no ? We have some main projects going well since people are independent and work as a team but we also have a lot of free electrons we could catalyse in something bigger ? WDYT ?<br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Luc (5 min)<br><br />
Update from Yann (1min to 10min)<br><br />
:: Where are we going ?<br />
:: How do we avoid UC becoming just another coworking?<br />
::: Do we want to put energy into this when I (Yann) feel no return whatsoever and mostly a loss of time and energy ?<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
- BSO title obtained<br />
- Wall and renovation problems.<br />
:: When are those renovations due ?<br />
:: What do we do with the material ?<br />
:: Sam was talking about amovible walls<br />
:: Yann: PhD and followings<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
* Web app architecture (Marco). It automagically recreates the graphs as new beers are introduced. http://decodedbeer-twoba.rhcloud.com/polls/beerchart_json/<br />
** The database works well back-end.<br />
** Help needed on Front-end (design). (Vanessa?)<br />
** Help needed on algorithms to mangle data. (Jonathan keen to do some work now that the PhD has been defended)<br />
<br />
*Bench work<br />
** Work done:<br />
** 96 beer pellets (Gianpaolo)<br />
** 96 DNA extractions (Gabrielle)<br />
** 192 PCRs: 96 x 16s bacteria, 96 x ITS fungi (Nicolas), --> sent to genomic facility for sequencing.<br />
** Only 39 PCR (ITS) are OK for sequencing.<br />
** New team member Isabelle, keen to work on the bench from October 1st.<br />
<br />
*QC at Hackuarium<br />
** Probably need for a more clean area to work with DNA (Gustavo, see P1 discussion)<br />
** After a DNA extraction, do we have DNA? Is it pure or contaminated? (Open nanodrop: Sam, Jochem)<br />
** After a PCR, did the PCR worked? Has it the right band size?<br />
<br />
*Bento prototype returned to fabric. Final device expected in November.<br />
<br />
===Living Instruments===<br />
Update from Luc (5min)<br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
==Point 2: Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application will be submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] is October 15th.<br />
<br />
<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br />
<br />
==Point 3: Future partnerships==<br />
<br />
===EPFL - Engineering and Architect internships===<br />
<br />
<br />
===Mike Horn pole2pole===<br />
<br />
<br />
===Joint Outreach with SIB===<br />
<br />
Waiting for Marie-Claude Blatter to come back to us. Reminder email sent on 31.08.2016, answered on 01.09.2016 <br><br />
<br />
She discussed with Nicolas Guex et Marco Pagni (Vital It SIB). A brief summary is available [https://docs.google.com/document/d/1Lwz7kxW3JrjqMhE_uqvNy3y7QEowYo4WVx6jQJ8fzjc/edit?usp=sharing here].<br><br />
<br />
Ideas include RNA seq on samples to see if there are metabolically active species in clouds. A first sampling session would give an idea (on Oct. 1st with baloon launch?). SIB can only bring expertise in data analysis. The sequencing costs and handling would have to be done with another partner (UNIL, Keith Harshman / Fasteris).<br />
<br />
==Point 4: Finance==<br />
<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
Very proud to say we have several events already planned this autumn:<br />
<br />
<br />
Still need to plan the following events:<br />
<br />
===Molecular Biology day/evening===<br />
<br />
Luc (3 min)<br />
<br />
Was moved to mid-october so we can advertise for it at the [[Openhackuarium_vol._116 | Openhackuarium vol. 116]].<br />
<br />
*WHEN? '''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]]<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
===Machine learning for image recognition===<br />
<br />
*WHEN?<br />
*WHO? Yann P. + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? A friend of Yann P. wants to present and bring a concrete case where we can show how with a RasPi we can differentiate things. A useful tech if for ex we want to look at wastes sorting.<br />
<br />
==Point 5bis: Other Events==<br />
<br />
<br />
==Point 6: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
===presence tracker===<br />
Are we happy with that?<br />
<br />
===meetup===<br />
Are we happy with that?<br />
<br />
==Point 7: Hacktion==<br />
<br />
<br />
==Point 8: Replication==<br />
(Luc, 5 min)<br />
3 projects in 2017<br />
<br />
How far did we go? <br />
<br />
Open Nanodrop:<br />
* Octanis hacked the Ocean Optics Spectrometer (200-1100nm) already<br />
* UV light source (Yann?) and SMA905 UV fibre optic cables ([http://www.ebay.com/itm/SMA905-fiber-optic-connector-UV-ultraviolet-visible-laser-fiber-core-200um-/232004914961?hash=item3604920f11:g:xGMAAOSwkl5XfaK8 50usd ebay]) missing<br />
::* Yann: We can use the leds for sure. I still got several of them! I don't think we need optical fibers because we can use UV detectors. <br />
* Good for DNA QC in future P1 (-> 230nm, 260nm, 280nm)<br />
::* Yep of course it is necessary for quantification! We could have gone low-cost and rely on the gel intensity.<br />
<br />
[[File:Screen Shot 2016-08-18 at 15.55.26.png]]<br />
<br />
==Point 9: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=20160919_Board_meeting&diff=790820160919 Board meeting2016-09-19T07:58:40Z<p>Randogp: </p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Thursday, September 19th, 19.30-21.30pm<br />
<br><br />
Place: UniverCité<br />
<br />
=Composition=<br />
<br />
==Chair==<br />
*Luc as president<br />
<br />
==Present==<br />
* ...<br />
<br />
==Excused==<br />
* Sam<br />
* ...<br />
<br />
<br><br />
<br />
=Items=<br />
<br />
==Welcome address==<br />
(Luc, 2 min)<br />
<br />
==Point 0.1: public minutes==<br />
(Luc, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 0.2: Actionables==<br />
<br />
To members: <br><br />
-> send invoice for membership fee using [http://www.smallinvoice.ch/CH/en/home/Create-and-manage-invoices-and-offers-for-free.html Smallinvoice.ch] -> Luc (up to end July, then for 3 months) -> Luc<br />
<br><br />
-> make a slide to insist at OH that people should talk about the place and advertise for it and become members (and how) -> Luc<br />
<br><br />
<br />
To board members: <br><br />
-> Email board member and ask for dedication (2 hours a week + list of tasks) -> Luc to present at this board meeting <br><br />
-> Every board member should really take care of motivating members -> let's insist this time again<br><br />
-> Implement [https://en.wikipedia.org/wiki/Performance_indicator key performance indicators] until the next General Assembly on Wednesday 14th December 2016 (Gianpaolo and Sam are are charge of monitoring [[KPI | here]]) <br><br />
-> Send a summary with membership fees and conditions <br><br />
-> Profile for 2 interns, including tasks and skills <br><br />
-> List of projects that need help, list of tasks <br><br />
-> Plan GA OH #128 Wed. 14th December <br><br />
-> Next board meetings 19th of Sept. 24th October, 28th November -> Luc sent a reminder on 31st August to board members<br><br />
-> Next [[Openhackuarium_vol._128 | General Assembly]] on Wednesday December 14th (during Openhackuarium #128, followed by a fondue). <br><br />
<br />
Luc asks Marc about the projector -> email sent on 31.08.2016<br><br />
Luc clarifies the role of Juliette and how we can help her help us <br><br />
Anne-Laure makes a list of people to invite in 2017 <br><br />
Every board member sends an email/call to 2-3 active projects and check where they are and report at the next board meeting, what is stopping you, what would you need to come more often <br><br />
Luc asks Cyanoguard what he expects from the lab. <br><br />
Luc asks Luc about bioreactor documentation -> done, Luc still needs to insist to make sure it is now completed properly<br><br />
Sam asks Marion about Air Sampler on OH. <br><br />
Make sure we have a list of tasks to get people on board -> post-its with projects and tasks so that people can sign-up. <br><br />
Sam gets RFID stickers to attach to the main card. <br><br />
Sam makes primary twitter channel and see how many unfollow (1month). <br><br />
Luc sends Pi workshop tweets. Done <br><br />
Yann sends Facebook message with Hackuarium. <br><br />
<br />
==Point 1: Project Updates==<br />
<br />
It is important that every board member stays up to date with all main projects. Add link when relevant. <br><br />
<br />
===Hackuarium in general===<br />
'''Yann (5min)'''<br />
*Board: Redefine roles: Board members have a task and commit to it, they invest time and energy in a tangible way.<br />
*Association: Maybe I'm just the one losing track but I have the feeling things are not moving or are just going randomly in any direction<br />
::* Do we try to set ourselves a goal as an association and try to push most of the members in this direction ? If we want to make an impact we have to pool our ressources no ? We have some main projects going well since people are independent and work as a team but we also have a lot of free electrons we could catalyse in something bigger ? WDYT ?<br />
<br />
===UniverCité Community meeting===<br />
<br />
Update from Luc (5 min)<br><br />
Update from Yann (1min to 10min)<br><br />
:: Where are we going ?<br />
:: How do we avoid UC becoming just another coworking?<br />
::: Do we want to put energy into this when I (Yann) feel no return whatsoever and mostly a loss of time and energy ?<br />
<br />
===P1 implementation===<br />
Update from Yann P. (5min)<br />
- BSO title obtained<br />
- Wall and renovation problems.<br />
:: When are those renovations due ?<br />
:: What do we do with the material ?<br />
:: Sam was talking about amovible walls<br />
:: Yann: PhD and followings<br />
<br />
===BeerDeCoded===<br />
Update from Gianpaolo (5min) <br><br />
<br />
===Living Instruments===<br />
Update from Luc (5min)<br />
<br />
===Octanis===<br />
Update from Sam (5min)<br />
<br />
==Point 2: Funding Applications==<br />
<br />
===Loterie Romande===<br />
<br />
A new application will be submitted at the Loterie Romande for 20'000 CHF. [https://www.entraide.ch/fr/vaud/delais-et-calendrier Deadline] is October 15th.<br />
<br />
<br />
<br />
===Others?===<br />
<br />
Luc has a list [https://docs.google.com/document/d/1KbIgTAQuySfRygdDwDdtkLIylVrHP0VSM6dJL7LoAP4/edit?usp=sharing here], and will keep writing emails as soon as he has time.<br />
<br />
==Point 3: Future partnerships==<br />
<br />
===EPFL - Engineering and Architect internships===<br />
<br />
<br />
===Mike Horn pole2pole===<br />
<br />
<br />
===Joint Outreach with SIB===<br />
<br />
Waiting for Marie-Claude Blatter to come back to us. Reminder email sent on 31.08.2016, answered on 01.09.2016 <br><br />
<br />
She discussed with Nicolas Guex et Marco Pagni (Vital It SIB). A brief summary is available [https://docs.google.com/document/d/1Lwz7kxW3JrjqMhE_uqvNy3y7QEowYo4WVx6jQJ8fzjc/edit?usp=sharing here].<br><br />
<br />
Ideas include RNA seq on samples to see if there are metabolically active species in clouds. A first sampling session would give an idea (on Oct. 1st with baloon launch?). SIB can only bring expertise in data analysis. The sequencing costs and handling would have to be done with another partner (UNIL, Keith Harshman / Fasteris).<br />
<br />
==Point 4: Finance==<br />
<br />
<br />
==Point 5: Upcoming OpenHackuarium nights==<br />
<br />
It is important that we announce events and stick to the plan. We already discussed the issue at the last board meeting and little has been done.<br />
<br />
===Already planned===<br />
<br />
Very proud to say we have several events already planned this autumn:<br />
<br />
<br />
Still need to plan the following events:<br />
<br />
===Molecular Biology day/evening===<br />
<br />
Luc (3 min)<br />
<br />
Was moved to mid-october so we can advertise for it at the [[Openhackuarium_vol._116 | Openhackuarium vol. 116]].<br />
<br />
*WHEN? '''2016.10.19''' Wednesday 19:30-22:00: [[Openhackuarium_vol._120 | Openhackuarium vol. 120]]<br />
*WHO? Luc + Gianpaolo + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? Discussion around the state of the art as well as basic mol biol technologies.<br />
<br />
Topics to be included in the discussion:<br />
* DNA (5 min introduction)<br />
* Genes (5 min introduction)<br />
* GM (how it is applied in bacteria, years, cells, animal, plants)<br />
* CRISPR (5 min introduction to the technique, and why it's hype)<br />
* Biotech (5 min on why all this is relevant)<br />
<br />
===Machine learning for image recognition===<br />
<br />
*WHEN?<br />
*WHO? Yann P. + young researchers from neighbouring institutions. Let's make a list and contact them.<br />
*WHAT? A friend of Yann P. wants to present and bring a concrete case where we can show how with a RasPi we can differentiate things. A useful tech if for ex we want to look at wastes sorting.<br />
<br />
==Point 5bis: Other Events==<br />
<br />
<br />
==Point 6: Infrastructure & IT==<br />
(Sam, 5 min)<br />
<br />
===presence tracker===<br />
Are we happy with that?<br />
<br />
===meetup===<br />
Are we happy with that?<br />
<br />
==Point 7: Hacktion==<br />
<br />
<br />
==Point 8: Replication==<br />
(Luc, 5 min)<br />
3 projects in 2017<br />
<br />
How far did we go? <br />
<br />
Open Nanodrop:<br />
* Octanis hacked the Ocean Optics Spectrometer (200-1100nm) already<br />
* UV light source (Yann?) and SMA905 UV fibre optic cables ([http://www.ebay.com/itm/SMA905-fiber-optic-connector-UV-ultraviolet-visible-laser-fiber-core-200um-/232004914961?hash=item3604920f11:g:xGMAAOSwkl5XfaK8 50usd ebay]) missing<br />
::* Yann: We can use the leds for sure. I still got several of them! I don't think we need optical fibers because we can use UV detectors. <br />
* Good for DNA QC in future P1 (-> 230nm, 260nm, 280nm)<br />
::* Yep of course it is necessary for quantification! We could have gone low-cost and rely on the gel intensity.<br />
<br />
[[File:Screen Shot 2016-08-18 at 15.55.26.png]]<br />
<br />
==Point 9: Future Actionables==<br />
<br />
[[Category:Board Meetings]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7777Hacktion2016-08-23T12:39:14Z<p>Randogp: /* 11. August */</p>
<hr />
<div>Daily 40 min challenge. Format:<br />
<br />
*minutes 0'-20': brainstorm actionable items that can be done in 20 minutes by one person.<br />
*minutes 21'-40': execute one actionable item.<br />
<br />
==23. August==<br />
Present: Gianpaolo, Sam<br />
<br />
*DONE Created meetup page for OpenHackuarium 112 https://www.meetup.com/hackuarium/events/233556220/<br />
*DONE Create DNA extraction kit for La Petite Epicerie<br />
*Make Google Drive for Hackuarium<br />
<br />
==22. August==<br />
Present: Gianpaolo, Sam<br />
<br />
* Lab activity: Caffeine extraction (NileRed on youtube)<br />
* "open ndrop" in progress: light source and spectro is good, missing are fibres <br />
<br />
<br />
==19. August==<br />
Present: Sam<br />
<br />
* How can we invite EPFL members? vg<br />
<br />
==18. August==<br />
Present: Gianpaolo, Sam, Luc (exended during the Board Meeting http://wiki.hackuarium.ch/w/20160818_Board_meeting)<br />
<br />
Actionable Items:<br />
* Board: Gianpaolo to share social media strategy.<br />
* Board: Define the medium for a list of open hardware projects.<br />
* Board: Gianpaolo to communicate with Yann about P1 inauguration.<br />
* Board: Luc email to Germany for DNA, when done, Gianpaolo activates Rachel & Roger.<br />
* Board: list of DNA things for Mike Horn (Gianpaolo, Luc).<br />
* Board: put the "petite epicerie" catalog on the wiki.<br />
* Board: create checklist of actions to do to advertise UC/Hackuarium events.<br />
**FB<br />
**tweet<br />
**meetup<br />
**A4 on the MC wall<br />
**post on MC private FB (Gianpaolo)<br />
**post on Hackuarium Slack<br />
**post on Fixme Slack<br />
**reimbursement google form<br />
<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
*send to juliette specifications for each OH for the google calendar.<br />
* update hackuarium webpage with correct event link http://wiki.hackuarium.ch/w/Main_Page<br />
* define next OH 112 and 113<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*DONE make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7775Hacktion2016-08-23T12:07:39Z<p>Randogp: /* 23. August */</p>
<hr />
<div>Daily 40 min challenge. Format:<br />
<br />
*minutes 0'-20': brainstorm actionable items that can be done in 20 minutes by one person.<br />
*minutes 21'-40': execute one actionable item.<br />
<br />
==23. August==<br />
Present: Gianpaolo, Sam<br />
<br />
*DONE Created meetup page for OpenHackuarium 112 https://www.meetup.com/hackuarium/events/233556220/<br />
*DONE Create DNA extraction kit for La Petite Epicerie<br />
<br />
==22. August==<br />
Present: Gianpaolo, Sam<br />
<br />
* Lab activity: Caffeine extraction (NileRed on youtube)<br />
* "open ndrop" in progress: light source and spectro is good, missing are fibres <br />
<br />
<br />
==19. August==<br />
Present: Sam<br />
<br />
* How can we invite EPFL members? vg<br />
<br />
==18. August==<br />
Present: Gianpaolo, Sam, Luc (exended during the Board Meeting http://wiki.hackuarium.ch/w/20160818_Board_meeting)<br />
<br />
Actionable Items:<br />
* Board: Gianpaolo to share social media strategy.<br />
* Board: Define the medium for a list of open hardware projects.<br />
* Board: Gianpaolo to communicate with Yann about P1 inauguration.<br />
* Board: Luc email to Germany for DNA, when done, Gianpaolo activates Rachel & Roger.<br />
* Board: list of DNA things for Mike Horn (Gianpaolo, Luc).<br />
* Board: put the "petite epicerie" catalog on the wiki.<br />
* Board: create checklist of actions to do to advertise UC/Hackuarium events.<br />
**FB<br />
**tweet<br />
**meetup<br />
**A4 on the MC wall<br />
**post on MC private FB (Gianpaolo)<br />
**post on Hackuarium Slack<br />
**post on Fixme Slack<br />
**reimbursement google form<br />
<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
*send to juliette specifications for each OH for the google calendar.<br />
* update hackuarium webpage with correct event link http://wiki.hackuarium.ch/w/Main_Page<br />
* define next OH 112 and 113<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*DONE make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7773Hacktion2016-08-23T10:53:14Z<p>Randogp: </p>
<hr />
<div>Daily 40 min challenge. Format:<br />
<br />
*minutes 0'-20': brainstorm actionable items that can be done in 20 minutes by one person.<br />
*minutes 21'-40': execute one actionable item.<br />
<br />
==23. August==<br />
Present:<br />
<br />
*Created meetup page for OpenHackuarium 112 https://www.meetup.com/hackuarium/events/233556220/<br />
<br />
==22. August==<br />
Present: Gianpaolo, Sam<br />
<br />
* Lab activity: Caffeine extraction (NileRed on youtube)<br />
* "open ndrop" in progress: light source and spectro is good, missing are fibres <br />
<br />
<br />
==19. August==<br />
Present: Sam<br />
<br />
* How can we invite EPFL members? vg<br />
<br />
==18. August==<br />
Present: Gianpaolo, Sam, Luc (exended during the Board Meeting http://wiki.hackuarium.ch/w/20160818_Board_meeting)<br />
<br />
Actionable Items:<br />
* Board: Gianpaolo to share social media strategy.<br />
* Board: Define the medium for a list of open hardware projects.<br />
* Board: Gianpaolo to communicate with Yann about P1 inauguration.<br />
* Board: Luc email to Germany for DNA, when done, Gianpaolo activates Rachel & Roger.<br />
* Board: list of DNA things for Mike Horn (Gianpaolo, Luc).<br />
* Board: put the "petite epicerie" catalog on the wiki.<br />
* Board: create checklist of actions to do to advertise UC/Hackuarium events.<br />
**FB<br />
**tweet<br />
**meetup<br />
**A4 on the MC wall<br />
**post on MC private FB (Gianpaolo)<br />
**post on Hackuarium Slack<br />
**post on Fixme Slack<br />
**reimbursement google form<br />
<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
*send to juliette specifications for each OH for the google calendar.<br />
* update hackuarium webpage with correct event link http://wiki.hackuarium.ch/w/Main_Page<br />
* define next OH 112 and 113<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*DONE make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Openhackuarium_vol._112&diff=7761Openhackuarium vol. 1122016-08-22T07:20:57Z<p>Randogp: Created page with "* Main language this week: '''English/French'''<br> * Theme : '''biohacking updates from our members''' * Date : '''Wednesday, August 24th, 2016. Doors open at 7.00pm''', pres..."</p>
<hr />
<div>* Main language this week: '''English/French'''<br><br />
* Theme : '''biohacking updates from our members'''<br />
* Date : '''Wednesday, August 24th, 2016. Doors open at 7.00pm''', presentations at 7.30 pm<br />
<br><br />
==MC Ouverture==<br />
<br />
Every wednesday we invite one MassChallenge startup to present what they are doing upstairs and what technology/diybio challenges they are facing.<br><br />
Tonight we will have Matthiew Hall from Simplicity Bio.<br />
<br />
==Project updates from members==<br />
<br />
Members will update us on their diybio projects, what is going well, what is stopping them, how can we help/<br><br />
<br />
==Free slot for our Meetup visitors==<br />
<br />
<br />
==Links==<br />
<br />
<br />
==Practical Info (access, map, etc.)==<br />
cf. [[Open_Hackuarium|#OpenHackuarium]]<br />
<br />
[[Category:Events]]<br />
[[Category:OpenHackuarium]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7752Hacktion2016-08-18T20:10:01Z<p>Randogp: /* 18. August */</p>
<hr />
<div>Daily 40 min challenge. Format:<br />
<br />
*minutes 0'-20': brainstorm actionable items that can be done in 20 minutes by one person.<br />
*minutes 21'-40': execute one actionable item.<br />
<br />
<br />
==18. August==<br />
Present: Gianpaolo, Sam, Luc (exended during the Board Meeting http://wiki.hackuarium.ch/w/20160818_Board_meeting)<br />
<br />
Actionable Items:<br />
* Board: Gianpaolo to share social media strategy.<br />
* Board: Define the medium for a list of open hardware projects.<br />
* Board: Gianpaolo to communicate with Yann about P1 inauguration.<br />
* Board: Luc email to Germany for DNA, when done, Gianpaolo activates Rachel & Roger.<br />
* Board: list of DNA things for Mike Horn (Gianpaolo, Luc).<br />
* Board: put the "petite epicerie" catalog on the wiki.<br />
* Board: create checklist of actions to do to advertise UC/Hackuarium events.<br />
**FB<br />
**tweet<br />
**meetup<br />
**A4 on the MC wall<br />
**post on MC private FB (Gianpaolo)<br />
**post on Hackuarium Slack<br />
**post on Fixme Slack<br />
<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
*send to juliette specifications for each OH for the google calendar.<br />
* update hackuarium webpage with correct event link http://wiki.hackuarium.ch/w/Main_Page<br />
* define next OH 112 and 113<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*DONE make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7747Hacktion2016-08-18T19:58:14Z<p>Randogp: /* 18. August */</p>
<hr />
<div>Daily 40 min challenge. Format:<br />
<br />
*minutes 0'-20': brainstorm actionable items that can be done in 20 minutes by one person.<br />
*minutes 21'-40': execute one actionable item.<br />
<br />
<br />
==18. August==<br />
Present: Gianpaolo, Sam, Luc (exended during the Board Meeting http://wiki.hackuarium.ch/w/20160818_Board_meeting)<br />
<br />
Actionable Items:<br />
* Board: Define the medium for a list of open hardware projects.<br />
* Board: Gianpaolo to communicate with Yann about P1 inauguration.<br />
* Board: Luc email to Germany for DNA, when done, Gianpaolo activates Rachel & Roger.<br />
* Board: list of DNA things for Mike Horn (Gianpaolo, Luc).<br />
* Board: put the "petite epicerie" catalog on the wiki.<br />
* Board: create checklist of actions to do to advertise UC/Hackuarium events.<br />
**FB<br />
**tweet<br />
**meetup<br />
**A4 on the MC wall<br />
**post on MC private FB (Gianpaolo)<br />
**post on Hackuarium Slack<br />
**post on Fixme Slack<br />
<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
*send to juliette specifications for each OH for the google calendar.<br />
* update hackuarium webpage with correct event link http://wiki.hackuarium.ch/w/Main_Page<br />
* define next OH 112 and 113<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*DONE make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7735Hacktion2016-08-18T19:23:56Z<p>Randogp: /* 18. August */</p>
<hr />
<div>Daily 40 min challenge. Format:<br />
<br />
*minutes 0'-20': brainstorm actionable items that can be done in 20 minutes by one person.<br />
*minutes 21'-40': execute one actionable item.<br />
<br />
<br />
==18. August==<br />
Present: Gianpaolo, Sam, Luc (exended during the Board Meeting)<br />
<br />
Actionable Items:<br />
* Luc email to Germany for DNA, when done, Gianpaolo activates Rachel & Roger<br />
* list of DNA things for Mike Horn (Gianpaolo, Luc)<br />
* put the "petite epicerie" catalog on the wiki<br />
* create checklist of actions to do to advertise UC/Hackuarium events<br />
**FB<br />
**tweet<br />
**meetup<br />
**A4 on the MC wall<br />
**post on MC private FB (Gianpaolo)<br />
**post on Hackuarium Slack<br />
**post on Fixme Slack<br />
<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
*send to juliette specifications for each OH for the google calendar.<br />
* update hackuarium webpage with correct event link http://wiki.hackuarium.ch/w/Main_Page<br />
* define next OH 112 and 113<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*DONE make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7674Hacktion2016-08-18T12:12:54Z<p>Randogp: </p>
<hr />
<div>==18. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable Items:<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
*send to juliette specifications for each OH for the google calendar.<br />
* update hackuarium webpage with correct event link http://wiki.hackuarium.ch/w/Main_Page<br />
* define next OH 112 and 113<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*DONE make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Category:Bachelor-Master-PhD_Projects&diff=7673Category:Bachelor-Master-PhD Projects2016-08-18T12:12:21Z<p>Randogp: </p>
<hr />
<div>In collaboration with Swiss universities, we facilitate the experimental work for students of Bachelor and Master offering an accessible 24/7 laboratory and a multidisciplinary sounding board.<br />
<br />
==Graduated with us==<br />
*Vanessa Lorenzo from HEAD Geneva, Master in Interaction Design 2016<br />
*Nicola Masserey from EPFL, Bachelor in Electrical Engineering 2016 --> see page on [http://wiki.octanis.org/orb/stereoscopy Octanis' wiki]<br />
*Michal Masternak from University of Lausanne, Master in Anthropology 2015<br />
*Alexandre Gubert from EPFL, Master in Mechanical Engineering 2016 (internship)</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Category:Bachelor-Master-PhD_Projects&diff=7672Category:Bachelor-Master-PhD Projects2016-08-18T11:32:56Z<p>Randogp: /* Graduated with us */</p>
<hr />
<div>In collaboration with Swiss universities, we facilitate the experimental work for students of Bachelor and Master offering an accessible 24/7 laboratory and a multidisciplinary sounding board.<br />
<br />
==Graduated with us==<br />
*Vanessa Lorenzo from HEAD Geneva, Master in Interaction Design 2016<br />
*Nicola Masserey from EPFL, Bachelor in Electrical Engineering 2016 --> see page on [http://wiki.octanis.org/orb/stereoscopy Octanis' wiki]<br />
*Michal Masternak from University of Lausanne, Master in Anthropology 2015</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7671Hacktion2016-08-18T11:28:00Z<p>Randogp: /* 18. August */</p>
<hr />
<div>==18. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable Items:<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7670Hacktion2016-08-18T10:37:53Z<p>Randogp: </p>
<hr />
<div>==18. August==<br />
Present:<br />
<br />
* create a wiki page in http://wiki.hackuarium.ch/w/Category:Board_Meetings with the notes of this old board meeting https://hackuarium.hackpad.com/Notes-Board-Meeting-du-12.01.2015-kAe0OrhmSxO<br />
<br />
==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Hacktion&diff=7669Hacktion2016-08-18T10:35:02Z<p>Randogp: /* 16. August */</p>
<hr />
<div>==17. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE book MC conference room for our board meeting<br />
*DONE find new invitees for next openhackuariums<br />
*What Hackuarium project can we bring at MakerFaire Zurich?<br />
==16. August==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
*DONE make a wiki page to place the master defenses and bachelor projects etc. http://wiki.hackuarium.ch/w/Category:Bachelor-Master-PhD_Projects<br />
*post to newsletter: reaction to survey<br />
*document microgrants<br />
*make a page to document the [[KPI]]<br />
<br />
==15. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE stick stickers<br />
* study customers opinion<br />
* DONE sell the ticket for workshop<br />
* board meeting topic: how to become member?<br />
* targets for p1:<br />
** pre-seed startups -> MC<br />
** iGEM teams -> Swiss universities<br />
** biohacking academy -> Waag<br />
* how to operate <br />
* smallinvoice<br />
<br />
==12. August ==<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Get "buy now" links for: <br />
** diy competent bacteria kit 12 CHF [https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency NEB]<br />
** plasmid dna extraction kit 2 CHF [https://www.carlroth.com/ch/en/Life-Science/Molecular-Biology/DNA--RNA-Isolation/Extraction-Systems/Roti%3Csup%3E%C2%AE%3C-sup%3E-Prep-Plasmid-MINI/p/000000080000d57300050023_en Carl Roth]<br />
** [http://www.ebay.com/itm/L298P-Shield-R3-DC-Motor-Driver-Module-2A-H-Bridge-2-way-For-Arduino-UNO-2560-NW-/161276647674?hash=item258cd658fa:g:NkMAAOSwuzRXdemM fluid pumping kit] (other source [http://www.ebay.com/itm/Dosing-Pump-Peristaltic-Pump-With-42-Stepper-Motor-Tubing-Hose-Pump-Aquarium-Lab-/291823200083?hash=item43f204a753:g:Q1kAAOSwWfFXjyh2 here])<br />
** [http://www.ebay.com/itm/UNO-R3-development-board-microcontroller-2012-MEGA328P-ATMEGA16U2-Arduino-Compat/291842874529?_trksid=p2047675.c100005.m1851&_trkparms=aid%3D222007%26algo%3DSIC.MBE%26ao%3D1%26asc%3D38052%26meid%3Dc01965d43111460bb1426e593a48b5d9%26pid%3D100005%26rk%3D4%26rkt%3D6%26sd%3D331937132525 arduino starter kit] (other source [http://www.ebay.com/itm/1pcs-Breadboard-Power-Supply-Module-5V-3-3V-For-Arduino-No-Breadboard-/400985395242?hash=item5d5c97902a:g:gLQAAMXQeW5TbZWT here])<br />
** raspberry pi starter kit (rs-online.ch RS: 896-8660, RS: 917-6317, RS: 909-8126 = 60CHF/kit)<br />
<br />
==11. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* Define the job profile of the operations manager<br />
* DONE Add Løding to meetup Openhackuarium and tell Yann to put on FB<br />
* Find out how to get free fridge from dechetterie: contacts [http://www.20min.ch/ro/news/vaud/story/Redonner-vie-aux-objets-valables-des-dechetteries-13363417 here]<br />
* Update meetup with OH for dummies<br />
* Where to get new projects for hackuarium? Offer them P1,makerspace,fridge... -> la grange, hub, other hacker/makerspaces...<br />
* Impact Hub ZH<br />
* EPFL innovation park<br />
* Blue Lion ZH<br />
* Technopark ZH<br />
* Aarau (Aargau Services)<br />
* Startup weekend (3-6months space to incubate project)<br />
* Epfl<br />
* Unil <br />
* Imd<br />
* Heig.vd<br />
* Ecal<br />
* Ehl<br />
* Chuv<br />
* Sib<br />
* Isrec<br />
* Ludwig<br />
<br />
* DONE Photo Wall (google form + word template A5)<br />
* Prenom<br />
* Nom<br />
* Coords (facebook, twitter, www)<br />
* Keywords<br />
<br />
==8. August ==<br />
Present: Gianpaolo<br />
<br />
Actionable items<br />
* DONE Prepare an inbox area for parcels when arrive to be identified as "coming"<br />
* DONE Add category board meeting to the board meeting minutes. (Gianpaolo, done: http://wiki.hackuarium.ch/w/Category:Board_Meetings)<br />
<br />
== 5. August ==<br />
<br />
Present: Gianpaolo and Sam<br />
<br />
Actionable items<br />
* DONE Order rectangular stickers with left/right/top arrow for signage downstairs<br />
* DONE Put on meetup next openhackuarium nights (Sam)<br />
* Define minimum list for the fridge<br />
** http://www.micrologylabs.com/products.php?product_id=7<br />
** serigraphy stuff<br />
** ethanol<br />
** acetone<br />
** pipette tips<br />
** fridge with glass vitrine: for example [http://www.anibis.ch/fr/m%C3%A9nage-~-maison-appareils-de-cuisine-r%C3%A9frig%C3%A9rateurs--299/r%C3%A9frig%C3%A9rateurs-vitrine-d'occasion--15338043.aspx here]<br />
* DONE Gianpaolo to invite some startup from MC to OpenHackuarium 110 [https://trello.com/b/kYxHjJ1i/openhackuarium-invite-list list on Trello]<br />
<br />
<br />
Review:<br />
* Flyer: see flyer.pdf<br />
* meetup.com/hackuarium (review Gianpaolo, then announce)<br />
* KPI: grafana<br />
<br />
== 4. August ==<br />
<br />
Present: Gianpaolo, Sam<br />
<br />
Actionable items:<br />
* DONE Stickers (Gianpaolo orders today) (Ordered [https://www.moo.com/share/jkb6d8 here 100 stickers 30 CHF], expected delivery August 26th)<br />
* Explainer Flyer (Sam will start today)<br />
* DONE Wall: People & Projects (Gianpaolo wants to start today)<br />
* DONE OpenHackuarium on https://www.meetup.com/ (Sam will create a page today)<br />
* DONE Definition of key performance indicators (Sam will have look)<br />
<br />
Backlog:<br />
<br />
<br />
* Newsletter à la Hub: Events, stories & co (see #collection_bin)<br />
* Posting stats on Slack "we've passed 30k"<br />
* Start looking for Operations Assistant for Hackuarium (not remunerated). Inspiration: [https://sictic.recruiterbox.com/jobs/fk06d7g?utm_source=Impact+Hub+Z%C3%BCrich+News&utm_campaign=c3b2524300-IHZ_News_from_31_07_16&utm_medium=email&utm_term=0_e3f6145765-c3b2524300-371373529 SICTIC] and [http://www.indeed.ch/cmp/www.XOBAEDI.com-(Swiss-Start--Up)/jobs/Project-Start-Coordinator-ad743430df426d69?q=xobaedi XOBAEDI] job announcements.<br />
* Inviting all people that we can (recurring as well as new) for tours (like we did for Patchwork) etc.<br />
<br />
[[Category:Work In Progress]]</div>Randogphttps://wiki.hackuarium.ch/index.php?title=Category:Bachelor-Master-PhD_Projects&diff=7668Category:Bachelor-Master-PhD Projects2016-08-18T10:34:21Z<p>Randogp: Created page with "In collaboration with Swiss universities, we facilitate the experimental work for students of Bachelor and Master offering an accessible 24/7 laboratory and a multidisciplinar..."</p>
<hr />
<div>In collaboration with Swiss universities, we facilitate the experimental work for students of Bachelor and Master offering an accessible 24/7 laboratory and a multidisciplinary sounding board.<br />
<br />
==Graduated with us==<br />
*Vanessa Lorenzo from HEAD Geneva, Master in Interaction Design 2016<br />
* Michal Masternak from University of Lausanne, Master in Anthropology 2015</div>Randogp