https://wiki.hackuarium.ch/api.php?action=feedcontributions&user=Dan.fhg&feedformat=atomHackuarium - User contributions [en]2024-03-28T18:08:40ZUser contributionsMediaWiki 1.35.3https://wiki.hackuarium.ch/index.php?title=Instru_Electroporator&diff=9854Instru Electroporator2017-11-02T09:52:26Z<p>Dan.fhg: </p>
<hr />
<div>=BioRad instrumentation=<br />
We are currently using this set up (see picture on right)<br />
== Instruction Manuals ==<br />
[[File:IMG 0989.JPG |580px|thumb|right| BioRad instrumentation]]<br />
<br />
[[File:Gene_Pulser_Electroporator_Operating_Instructions.pdf]]<br />
<br />
<br />
[[File:Electroporation_and_Pulse_Controller_Instruction_Manual.pdf]]<br />
<br />
=others=<br />
<br />
There are several other electroporation units</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=File:IMG_0989.JPG&diff=9799File:IMG 0989.JPG2017-11-01T15:29:55Z<p>Dan.fhg: Image of 2017-10-31 electroporator set up</p>
<hr />
<div>Image of 2017-10-31 electroporator set up</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Instru_Electroporator&diff=9798Instru Electroporator2017-11-01T15:26:45Z<p>Dan.fhg: </p>
<hr />
<div><br />
[[File:Gene_Pulser_Electroporator_Operating_Instructions.pdf]]<br />
<br />
[[File:Electroporation_and_Pulse_Controller_Instruction_Manual.pdf]]<br />
<br />
[[File:Electroporation_and_Pulse_Controller_Instruction_Manual.pdf | Hi ]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Instru_Electroporator&diff=9797Instru Electroporator2017-11-01T15:13:13Z<p>Dan.fhg: </p>
<hr />
<div>[[File:Example.jpg]]<br />
[[File:Gene_Pulser_Electroporator_Operating_Instructions.pdf]]<br />
[[File:Electroporation_and_Pulse_Controller_Instruction_Manual.pdf]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Instru_Electroporator&diff=9796Instru Electroporator2017-11-01T15:10:16Z<p>Dan.fhg: Created page with "File:Electroporation_and_Pulse_Controller_Instruction_Manual.pdf File:Gene_Pulser_Electroporator_Operating_Instructions.pdf"</p>
<hr />
<div>[[File:Electroporation_and_Pulse_Controller_Instruction_Manual.pdf]]<br />
<br />
[[File:Gene_Pulser_Electroporator_Operating_Instructions.pdf]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=File:Gene_Pulser_Electroporator_Operating_Instructions.pdf&diff=9795File:Gene Pulser Electroporator Operating Instructions.pdf2017-11-01T15:08:45Z<p>Dan.fhg: </p>
<hr />
<div></div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=File:Electroporation_and_Pulse_Controller_Instruction_Manual.pdf&diff=9794File:Electroporation and Pulse Controller Instruction Manual.pdf2017-11-01T15:08:08Z<p>Dan.fhg: </p>
<hr />
<div></div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Instru_Main&diff=9793Instru Main2017-11-01T15:01:56Z<p>Dan.fhg: /* Instrument List */</p>
<hr />
<div>== General Considerations == <br />
The lab is like a big shared flat with several dozens of people using it therefore it is important to follow some rules for everybody to be safe and the material to be correctly treated. <br><br />
- Every instrument on the outer part of the lab is common, everybody can use them.<br><br />
- Objects or instruments on the workzones and project shelves are personal. Don't use them without the owner's agreement.<br><br />
- As soon as you are done working with an instrument clean it and put it back to its place. <br><br />
- If it is not operating it is turned off. <br><br />
- Be considerate to others: If you just need to stir a solution take the simplest magnetic stiring plate not the fancy heating plate with feedback mechanism.<br><br />
- Last but not least: All the material you will use was brought by some members who had to take time and energy for you to enjoy it. Respect this. <br><br />
<br />
== Instrument List ==<br />
<br />
In this page you will find an alphabetical list of all the functional instruments we have in the Hackuarium space, how to operate them and as well safety measure you should be aware of.<br />
:<li>[[Instru_Autoclave | Autoclave]]<br />
:<li>[[Instru_Baths | Baths ]]<br />
:<li>[[Instru_Centrifuge | Centrifuge]]<br />
:<li>[[Instru_DNAElec | DNA Electrophoresis]]<br />
:<li>[[Instru_Electroporator | Electroporator]]<br />
:<li>[[Instru_Light | Light Source for Fibre Optics]]<br />
:<li>[[Instru_Fridge | Fridge / Freezer]]<br />
:<li>[[Instru_Furnace | Furnace]]<br />
:<li>[[Instru_Gel_Imager| Gel Imager]]<br />
:<li>[[Instru_HeatGun | Heat Gun]]<br />
:<li>[[Instru_HeatBlock | Heating Block]]<br />
:<li>[[Instru_HeatPlate | Heating Plates]]<br />
:<li>[[Instru_Hood | Hood]]<br />
:<li>[[Instru_Incubator | Incubator]]<br />
:<li>[[Instru_Microscopes | Microscopes]]<br />
:<li>[[Instru_PCR | PCR]]<br />
:<li>[[Instru_Pipettes | Pipettes ]]<br />
:<li>[[Instru_PlateReader | Plate Reader]]<br />
:<li>[[Instru_Rotavap | Rotavap]]<br />
:<li>[[Instru_Scales | Scales]]<br />
:<li>[[Instru_Sonicator | Sonicator]]<br />
:<li>[[Instru_SterileHood | Sterile Hood]]<br />
:<li>[[Instru_UVSpectro | UV Spectrometer]]<br />
:<li>[[Instru_Vortex | Vortex]]<br />
<br />
[[Category:Work in Progress]]<br />
[[Category:Instruments]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Instru_Main&diff=9792Instru Main2017-11-01T15:01:29Z<p>Dan.fhg: </p>
<hr />
<div>== General Considerations == <br />
The lab is like a big shared flat with several dozens of people using it therefore it is important to follow some rules for everybody to be safe and the material to be correctly treated. <br><br />
- Every instrument on the outer part of the lab is common, everybody can use them.<br><br />
- Objects or instruments on the workzones and project shelves are personal. Don't use them without the owner's agreement.<br><br />
- As soon as you are done working with an instrument clean it and put it back to its place. <br><br />
- If it is not operating it is turned off. <br><br />
- Be considerate to others: If you just need to stir a solution take the simplest magnetic stiring plate not the fancy heating plate with feedback mechanism.<br><br />
- Last but not least: All the material you will use was brought by some members who had to take time and energy for you to enjoy it. Respect this. <br><br />
<br />
== Instrument List ==<br />
<br />
In this page you will find an alphabetical list of all the functional instruments we have in the Hackuarium space, how to operate them and as well safety measure you should be aware of.<br />
:<li>[[Instru_Autoclave | Autoclave]]<br />
:<li>[[Instru_Electroporator | Electroporator]]<br />
:<li>[[Instru_Baths | Baths ]]<br />
:<li>[[Instru_Centrifuge | Centrifuge]]<br />
:<li>[[Instru_DNAElec | DNA Electrophoresis]]<br />
:<li>[[Instru_Light | Light Source for Fibre Optics]]<br />
:<li>[[Instru_Fridge | Fridge / Freezer]]<br />
:<li>[[Instru_Furnace | Furnace]]<br />
:<li>[[Instru_Gel_Imager| Gel Imager]]<br />
:<li>[[Instru_HeatGun | Heat Gun]]<br />
:<li>[[Instru_HeatBlock | Heating Block]]<br />
:<li>[[Instru_HeatPlate | Heating Plates]]<br />
:<li>[[Instru_Hood | Hood]]<br />
:<li>[[Instru_Incubator | Incubator]]<br />
:<li>[[Instru_Microscopes | Microscopes]]<br />
:<li>[[Instru_PCR | PCR]]<br />
:<li>[[Instru_Pipettes | Pipettes ]]<br />
:<li>[[Instru_PlateReader | Plate Reader]]<br />
:<li>[[Instru_Rotavap | Rotavap]]<br />
:<li>[[Instru_Scales | Scales]]<br />
:<li>[[Instru_Sonicator | Sonicator]]<br />
:<li>[[Instru_SterileHood | Sterile Hood]]<br />
:<li>[[Instru_UVSpectro | UV Spectrometer]]<br />
:<li>[[Instru_Vortex | Vortex]]<br />
<br />
[[Category:Work in Progress]]<br />
[[Category:Instruments]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9768HTGAA2017-10-27T12:29:24Z<p>Dan.fhg: /* Week 3 next generation synthesis */</p>
<hr />
<div><br />
== HTGAA ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
= HTGAA course 2017=<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
==Important Links==<br />
<br />
===Hackuarium Specific Links===<br />
Hackuarium Official Course Page: http://bio.fabcloud.io/hackuarium_2017/<br />
<br />
Hackuarium Working Assignments Folder: https://drive.google.com/open?id=0B26FGn9YdUxQVmZuaTBNNWlGR0k<br />
<br />
<br />
===General Course Links===<br />
<br />
Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
==Weekly topics / Experiments==<br />
<br />
=== wk1: Principles and Practices ===<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/principles_practices_hardware.html<br />
<br />
<br />
<br />
=== wk2: Bio Design / Plasmid digest===<br />
<br />
Theory class: George Church spoke about the capacity to modify biological material/organisms.<br />
<br />
Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html<br />
<br />
The protocol designed for this experiment is: <br />
<br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing<br />
<br />
The experiment was carried out on wednesday, 11th of of October 2017 with the help of Rachel.<br />
<br />
The gel prepared was 20 mL of 1% agarose in 1x TAE buffer. It was prepared by dissolving 0.2g agarose in 20 mL of a 1x TAE buffer solution in the microwave. Upon full dissolution of the agarose, the sample was allowed to cool a bit, SYBR safe dye was added, and the liquid cast into an old horizontal mini gel box tray. A special 'hack' with magnets kept the comb from going too deep into the liquid, as shown.<br />
[[File:Gel_Hack_HTGAA.jpg |400px|]]<br />
<br />
The Gel contained:<br />
<br />
=== wk3: next generation synthesis ===<br />
<br />
Theory task: design a gene with a detailed workflow of how to build it.<br />
<br />
Lab task: Recode a GFP plasmid to another variant of fluorescent protein using a golden gate assembly.<br />
<br />
Ideally this task should be started ASAP as soon as the oligos arrive for the golden gate assembly<br />
<br />
== Headline text ==</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9767HTGAA2017-10-27T12:29:01Z<p>Dan.fhg: /* Week 3 next generation synthesis */</p>
<hr />
<div><br />
== HTGAA ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
= HTGAA course 2017=<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
==Important Links==<br />
<br />
===Hackuarium Specific Links===<br />
Hackuarium Official Course Page: http://bio.fabcloud.io/hackuarium_2017/<br />
<br />
Hackuarium Working Assignments Folder: https://drive.google.com/open?id=0B26FGn9YdUxQVmZuaTBNNWlGR0k<br />
<br />
<br />
===General Course Links===<br />
<br />
Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
==Weekly topics / Experiments==<br />
<br />
=== wk1: Principles and Practices ===<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/principles_practices_hardware.html<br />
<br />
<br />
<br />
=== wk2: Bio Design / Plasmid digest===<br />
<br />
Theory class: George Church spoke about the capacity to modify biological material/organisms.<br />
<br />
Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html<br />
<br />
The protocol designed for this experiment is: <br />
<br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing<br />
<br />
The experiment was carried out on wednesday, 11th of of October 2017 with the help of Rachel.<br />
<br />
The gel prepared was 20 mL of 1% agarose in 1x TAE buffer. It was prepared by dissolving 0.2g agarose in 20 mL of a 1x TAE buffer solution in the microwave. Upon full dissolution of the agarose, the sample was allowed to cool a bit, SYBR safe dye was added, and the liquid cast into an old horizontal mini gel box tray. A special 'hack' with magnets kept the comb from going too deep into the liquid, as shown.<br />
[[File:Gel_Hack_HTGAA.jpg |400px|]]<br />
<br />
The Gel contained:<br />
<br />
=== Week 3 next generation synthesis ===<br />
<br />
Theory task: design a gene with a detailed workflow of how to build it.<br />
<br />
Lab task: Recode a GFP plasmid to another variant of fluorescent protein using a golden gate assembly.<br />
<br />
Ideally this task should be started ASAP as soon as the oligos arrive for the golden gate assembly<br />
<br />
== Headline text ==</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9717HTGAA2017-10-13T19:01:09Z<p>Dan.fhg: </p>
<hr />
<div><br />
== HTGAA ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
= HTGAA course 2017=<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
==Important Links==<br />
<br />
===Hackuarium Specific Links===<br />
Hackuarium Official Course Page: http://bio.fabcloud.io/hackuarium_2017/<br />
<br />
Hackuarium Working Assignments Folder: https://drive.google.com/open?id=0B26FGn9YdUxQVmZuaTBNNWlGR0k<br />
<br />
<br />
===General Course Links===<br />
<br />
Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
==Weekly topics / Experiments==<br />
<br />
=== wk1: Principles and Practices ===<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/principles_practices_hardware.html<br />
<br />
<br />
<br />
=== wk2: Bio Design / Plasmid digest===<br />
<br />
Theory class: George Church spoke about the capacity to modify biological material/organisms.<br />
<br />
Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html<br />
<br />
The protocol designed for this experiment is: <br />
<br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing<br />
<br />
The experiment was carried out on wednesday, 11th of of October 2017 with the help of Rachel.<br />
<br />
[[File:Gel_Hack_HTGAA.jpg]]<br />
<br />
The gel prepared was 20 mL of 1% agarose -1x TAE buffered gel. It was prepared by dissolving 0.2g of agarose inside 20 mL of a 1x TAE buffer solution. Upon boiling of the gel, SYBR safe dye was added and the liquid cast into the makeshift box.<br />
The Gel contained:<br />
<br />
<br />
== Week 3 next generation synthesis ==<br />
<br />
Theory task: design a gene with a detailed workflow of how to build it.<br />
<br />
Lab task: Recode a GFP plasmid to another variant of fluorescent protein using a golden gate assembly.<br />
<br />
Ideally this task should be started ASAP as soon as the oligos arrive for the golden gate assembly<br />
<br />
<br />
<br />
== Headline text ==</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9716HTGAA2017-10-13T15:05:23Z<p>Dan.fhg: </p>
<hr />
<div><br />
== HTGAA ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
= HTGAA course 2017=<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
==Important Links==<br />
<br />
===Hackuarium Specific Links===<br />
Hackuarium Official Course Page: http://bio.fabcloud.io/hackuarium_2017/<br />
<br />
Hackuarium Working Assignments Folder: https://drive.google.com/open?id=0B26FGn9YdUxQVmZuaTBNNWlGR0k<br />
<br />
<br />
===General Course Links===<br />
<br />
Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
==Weekly topics / Experiments==<br />
<br />
=== wk1: Principles and Practices ===<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/principles_practices_hardware.html<br />
<br />
<br />
<br />
=== wk2: Bio Design / Plasmid digest===<br />
<br />
Theory class: George Church spoke about the capacity to modify biological material/organisms.<br />
<br />
Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html<br />
<br />
The protocol designed for this experiment is: <br />
<br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing<br />
<br />
The experiment was carried out on wednesday, 11th of of October 2017 with the help of Rachel.<br />
<br />
[[File:Gel_Hack_HTGAA.jpg]]<br />
<br />
The gel prepared was 20 mL of 1% agarose -1x TAE buffered gel. It was prepared by dissolving 0.2g of agarose inside 20 mL of a 1x TAE buffer solution. Upon boiling of the gel, SYBR safe dye was added and the liquid cast into the makeshift box.<br />
The Gel contained:</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=File:HTGAA_theory_task_2_gel.JPG&diff=9715File:HTGAA theory task 2 gel.JPG2017-10-13T15:04:49Z<p>Dan.fhg: The gel that was prepared for the HTGAA lab task. It was allowed to run at 120V for two hours.
In order from left to right:
1kb ladder,YPP3 plasmid</p>
<hr />
<div>The gel that was prepared for the HTGAA lab task. It was allowed to run at 120V for two hours.<br />
In order from left to right:<br />
1kb ladder,YPP3 plasmid</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=File:Gel_Hack_HTGAA.jpg&diff=9714File:Gel Hack HTGAA.jpg2017-10-13T14:54:13Z<p>Dan.fhg: Gel prepared for HTGAA lab task number two.
The comb was held in place using strong magnets acting as support and ensuring appropriate height for the grooves.</p>
<hr />
<div>Gel prepared for HTGAA lab task number two.<br />
The comb was held in place using strong magnets acting as support and ensuring appropriate height for the grooves.</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9695HTGAA2017-10-06T10:05:14Z<p>Dan.fhg: /* Important Links */</p>
<hr />
<div><br />
== HTGAA ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
= HTGAA course 2017=<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
==Important Links==<br />
<br />
===Hackuarium Specific Links===<br />
Hackuarium Official Course Page: http://bio.fabcloud.io/hackuarium_2017/<br />
<br />
Hackuarium Working Assignments Folder: https://drive.google.com/open?id=0B26FGn9YdUxQVmZuaTBNNWlGR0k<br />
<br />
<br />
===General Course Links===<br />
<br />
Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
==Weekly topics / Experiments==<br />
<br />
=== wk1: Principles and Practices ===<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/principles_practices_hardware.html<br />
<br />
<br />
<br />
=== wk2: Bio Design / Plasmid digest===<br />
<br />
Theory class: George Church spoke about the capacity to modify biological material/organisms.<br />
<br />
Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html<br />
<br />
The protocol designed for this experiment is: <br />
<br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9694HTGAA2017-10-06T10:02:35Z<p>Dan.fhg: /* Weekly topics / Experiments */</p>
<hr />
<div><br />
== HTGAA ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
= HTGAA course 2017=<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
==Important Links==<br />
Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.<br />
<br />
Hackuarium Course Page: http://bio.fabcloud.io/hackuarium_2017/<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
==Weekly topics / Experiments==<br />
<br />
=== wk1: Principles and Practices ===<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/principles_practices_hardware.html<br />
<br />
<br />
<br />
=== wk2: Bio Design / Plasmid digest===<br />
<br />
Theory class: George Church spoke about the capacity to modify biological material/organisms.<br />
<br />
Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html<br />
<br />
The protocol designed for this experiment is: <br />
<br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9693HTGAA2017-10-06T09:42:33Z<p>Dan.fhg: </p>
<hr />
<div><br />
== HTGAA ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
= HTGAA course 2017=<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
==Important Links==<br />
Course page: http://bio.fabcloud.io/2017/ This includes all the weekly lectures and homework discussions in the left-hand sidebar. At the bottom there are additional learning resources.<br />
<br />
Hackuarium Course Page: http://bio.fabcloud.io/hackuarium_2017/<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
==Weekly topics / Experiments==<br />
<br />
=== wk2: Bio Design / Plasmid digest===<br />
<br />
Theory class: George Church spoke about the capacity to modify biological material/organisms.<br />
Lab Task: Design and verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
Link to weekly page: http://bio.fabcloud.io/2017/bio_design.html<br />
<br />
The protocol designed for this experiment is: <br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9692HTGAA2017-10-06T09:28:59Z<p>Dan.fhg: </p>
<hr />
<div><br />
== HTGAA course ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
=Course Documents and Links=<br />
http://bio.fabcloud.io/2017/<br />
<br />
Students:<br />
https://docs.google.com/spreadsheets/d/1WRnoyLbWejQslRvaRNfQBxJkjgmKWHNfFT9BOZqdEGM/edit#gid=0<br />
<br />
Curriculum:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
Recommended Hardware:<br />
https://docs.google.com/spreadsheets/d/1iu57Ct9NiPPVxzK_PBd_JlX4dlB68pQOxFCOnThnZPs/edit#gid=0<br />
<br />
Inventory:<br />
https://docs.google.com/spreadsheets/d/1YRWQRTBSgn1aUY-WVLG3K8riCbV-ZATtPElDXcY88gQ/edit#gid=580981494<br />
<br />
=Weekly topics / Experiments=<br />
<br />
== wk2: Plasmid digest==<br />
<br />
DNA modification is fundamental to design in synthetic biology. For this week's assignment, you will learn to read DNA sequence files and see physical changes you will make to DNA molecules.<br />
<br />
Lab Task: Verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
The protocol designed for this experiment is: <br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9691HTGAA2017-10-06T08:58:53Z<p>Dan.fhg: /* HTGAA course */</p>
<hr />
<div><br />
== HTGAA course ==<br />
<br />
From the official [http://bio.academany.org/:Link webpage]: Bio Academy is a Synthetic Biology Program directed by George Church, professor of Genetics at Harvard medical school. The Bio Academy is a part of the growing Academy of (almost) Anything, or the Academany. <br />
<br />
It's a 15 week experimental course covering the major Synthetic Biology applications today.<br />
<br />
2017 is the first year that the course is being taken by students at Hackuarium. The course is being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
== HTGAA week 2 homework Plasmid digest==<br />
<br />
DNA modification is fundamental to design in synthetic biology. For this week's assignment, you will learn to read DNA sequence files and see physical changes you will make to DNA molecules.<br />
<br />
Lab Task: Verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
The protocol designed for this experiment is: <br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9690HTGAA2017-10-06T08:43:41Z<p>Dan.fhg: /* HTGAA course */</p>
<hr />
<div><br />
== HTGAA course ==<br />
Course being followed by [[User:Dan | Daniel Hernandez]] and Thomas Jordan.<br />
<br />
== HTGAA week 2 homework Plasmid digest==<br />
<br />
DNA modification is fundamental to design in synthetic biology. For this week's assignment, you will learn to read DNA sequence files and see physical changes you will make to DNA molecules.<br />
<br />
Lab Task: Verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
The protocol designed for this experiment is: <br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9658HTGAA2017-10-04T17:48:43Z<p>Dan.fhg: /* HTGAA week 2 homework Plasmid digest */</p>
<hr />
<div><br />
== HTGAA course ==<br />
Course being followed by Dan Hernandez and Thomas Jordan.<br />
<br />
== HTGAA week 2 homework Plasmid digest==<br />
<br />
DNA modification is fundamental to design in synthetic biology. For this week's assignment, you will learn to read DNA sequence files and see physical changes you will make to DNA molecules.<br />
<br />
Lab Task: Verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
The protocol designed for this experiment is: <br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w/edit?usp=sharing</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9657HTGAA2017-10-04T17:47:08Z<p>Dan.fhg: /* HTGAA course */</p>
<hr />
<div><br />
== HTGAA course ==<br />
Course being followed by Dan Hernandez and Thomas Jordan.<br />
<br />
== HTGAA week 2 homework Plasmid digest==<br />
<br />
DNA modification is fundamental to design in synthetic biology. For this week's assignment, you will learn to read DNA sequence files and see physical changes you will make to DNA molecules.<br />
<br />
Lab Task: Verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
The protocol designed for this experiment is: <br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=HTGAA&diff=9656HTGAA2017-10-04T17:45:51Z<p>Dan.fhg: HTGAA Homework week 2</p>
<hr />
<div><br />
== HTGAA course ==<br />
<br />
== HTGAA week 2 homework Plasmid digest==<br />
<br />
DNA modification is fundamental to design in synthetic biology. For this week's assignment, you will learn to read DNA sequence files and see physical changes you will make to DNA molecules.<br />
<br />
Lab Task: Verify restriction digest of a circular DNA plasmid by gel electrophoresis. Compare your experimental result with what you would predict from the plasmid map.<br />
<br />
The protocol designed for this experiment is: <br />
https://docs.google.com/document/d/1Rs1ElrSjuLlmIEhpxZ6856ivZJBkXE4BnWnDYquZZ0w</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Main_Page&diff=9641Main Page2017-10-04T16:17:13Z<p>Dan.fhg: /* Active Projects */</p>
<hr />
<div>Welcome to the [http://www.hackuarium.ch/ Hackuarium] community's wiki.<br />
<br />
At Hackuarium, we want to bring biology (and biologists) to the world, and the real world back to biology. Our laboratory is an excuse to meet and discuss, build and develop ideas in a neutral, open, noncompetitive and not-for-profit environment.<br />
<br />
<div><br />
''[[Hackuarium | Cliquez ici pour accéder à la version française de ce wiki.]]'' <br />
</div><br />
<br />
This page was launched during our [[1st Ever Hackuarium Wiki Sprint]] that took place on 7 December 2014. As you might notice quickly, it is a work-in-progress.<br />
<br />
Scroll down and find out what this is all about!<br />
<br />
[[File:PosterHackuarium.png |580px|thumb|right|Hackuarium Poster (Not-for-printing version!!! see Section 7.9 [[Main_Page#Visual_Identity | Visual Identity]] for full size printing version)]] <br />
<br />
__TOC__<br />
<br />
<br><br />
<br />
=What is DIYbio?=<br />
DIYbio (Do-It-Yourself biology) is a movement that wants to free the practice of biological research and innovation from the institutional and industrial laboratories. Pursued both by amateurs and professional biologists, DIYbio is developing low-cost and low-tech solutions to problems identified by the community.<br />
<br />
The movement is characterised by an extremely diverse set of practices and participants. Some call themselves 'biohackers', in reference to the hacker culture.<br />
<br />
[http://DIYbio.org DIYbio.org] and [http://DIYbio.eu DIYbio.eu] are the portals of the international and european communities, respectively.<br />
<br />
For more information, visit the [http://www.wikipedia.org Wikipedia] page dedicated to [https://en.wikipedia.org/wiki/DIYbio DIYbio].<br />
<br><br />
DIT Research is a more recent expansion of this concept, to encompass trans-disciplinary explorations and projects, and not only biology. <br><br />
<br />
=What is Hackuarium?=<br />
<br />
Hackuarium is a not-for-profit association aiming at democratizing science through public engagement.<br />
<br><br />
<br />
Our laboratory in Renens (Switzerland) is open to anyone sharing the values of the association and who is dedicated to follow the [http://diybio.org/codes/draft-diybio-code-of-ethics-from-european-congress/ DIYbio Europe ethical guidelines].<br />
<br />
Our projects are initiated and carried out by scientists as well as non-scientists from a variety of background. They are passionate about tinkering with biology in particular, and technology in general. Some are engineers, architects, designers, IT and computer scientists or retired professionals, but others have no scientific education. They are mostly citizens interested in open and participatory research and innovation, outside the constrains of traditional institutions.<br />
<br />
Hackuarium members want to investigate new ways of carrying out interdisciplinary research and innovation, by making their results accessible (low-cost), simple and easily reproducible (low-tech) and by promoting an open source philosophy.<br />
<br />
=Where are we?=<br />
<br />
Hackuarium is proudly using infrastructures provided by [http://www.univercite.ch UniverCité], an unconventional innovation hub that opened in May 2014 in the IRL building in Renens. Our equipment is mostly upcycled material from institutions and industries from western Switzerland. We have documented our working environment as it has evolved.<br><br />
<br />
Between December 2015 and January 2017, UniverCité regrouped our laboratory, a workshop and coworking space.<br><br />
<br />
The original space was under construction work from January until June 2017 and We temporarily relocated on the first floor of the same building.<br><br />
<br />
We are back on the second floor of what is now called "Ateliers de Renens", Chemin du Closed 5, 1020 Renens.<br><br />
<br />
=Who are we?=<br />
<br />
Renens is a hopping hub - and our members reflect the diversity that is in and around Lausanne/Renens.<br />
<br><br />
[[People | Check us out]], and find out what makes Hackuarium a good mix.<br />
<br><br />
<br />
==Committee==<br />
<br />
The current [[Board | committee]] is composed of the following people:<br />
<br />
* Co - President: [[User: Vlorenzo | Vanessa Lorenzo]] (also Art and Design coordinator)<br />
* Co - President: [[User: Rachel | Rachel Aronoff]] (also Citizen Science coordinator)<br />
* Secretary: [[User: luchenry | Luc Henry]]<br />
* Lab Manager: [[User: Gustavo Santamaria| Gustavo Santamaria]]<br />
* Online promotion:[[User: hekkcess | Yann Pierson]]<br />
* Treasurer: [[User:Ana | Ana Roldan]]<br />
* System Administrator: [[User:Sam | Sam Sulaimanov]]<br />
* Talent scout and evangelist for Academy: [[User:Alpittet | Anne-Laure Pittet]]<br />
* Talent scout and evangelist for Entrepreneurs: [[User:Randogp | Gianpaolo Rando]]<br />
* Talent scout and evangelist: [[User:Dan | Daniel Hernandez]]<br />
* Community happiness, Ateliers de Renens Link: [[User: Shalf | Yann Heurtaux]]<br />
<br />
The Agenda of the last board meeting can be found [[http://wiki.hackuarium.ch/w/20170911_Board_Meeting here]].<br><br />
The Agenda of the next board meeting can be found [http://wiki.hackuarium.ch/w/20171004_Board_Meeting here] <br><br />
<br />
==Members and Membership==<br />
<br />
'''Do you have a project in mind? Do you want to just support us?'''<br />
<br />
Hackuarium operates on a membership basis, even though all events are open to anyone, including non-members.<br />
<br />
The monthly membership fee is 20 CHF. It gives 24/7 access to the lab.<br />
<br />
Please ask a member of the board if you want to join, and follow the directions below.<br />
<br />
===Want to join?===<br />
<br />
*We have a [https://docs.google.com/forms/d/e/1FAIpQLSdoyS8HmsuZACYp-q79pLzxR9BPA60HbyL6ZR8x7h7x3eqbhQ/viewform new form on the website], to fast-track joining the community, if you are keen, perhaps after a few #OpenHackuarium experiences (every Wednesday evening is open to the public).<br />
*We will help you with some '''helpful info''' [http://wiki.hackuarium.ch/w/I_m_new here: I'm new page]<br />
*Write an email to '''hello@hackuarium.ch''' and give us a short description of:<br />
**yourself, your interests, your background<br />
**what you would like to learn or do at Hackuarium<br />
** Tell us everything !! ;)<br />
<br />
==Friends of Hackuarium==<br />
<br />
Spaces:<br />
::<li>[[UniverCité]]<br />
::<li>[http://lapaillasse.org La Paillasse, Paris]<br />
::<li>[https://lapaillassaone.wordpress.com Paillasse Saône, Lyon]<br />
::<li>[http://foundry.bio The BioFoundry, Sydney]<br />
::<li>[http://www.bioquisitive.org.au Bioquisitive, Melbourne]<br />
<br />
Projects:<br />
::<li>[[Exodes_Urbains | Exodes Urbains]]<br />
::<li>[http://echopen.org/index.php?title=Main_Page EchOpen]<br />
::<li>[http://octanis.org Octanis]<br />
::<li>[http://hybridoa.org Hybridoa]<br />
<br />
=Events=<br />
<br />
<br><br />
<big>'''[[Open_Hackuarium | OpenHackuarium]]: Every Wednesday, 19:00-22:00'''</big><br />
<br />
Our lab and space are open to the public. Please come and have a chat! And if you have any questions [mailto:hello@hackuarium.ch get in touch!]<br />
<br />
==Upcoming Hackuarium Events==<br />
<br><br />
<br />
:<li>'''2017.10.04''' Wednesday 18:30-20:30: [[20171004_Board_Meeting | Board Meeting]]<br />
:<li>'''2017.09.27''' Wednesday 19:30-22:30: [[20170927_FilmMaking | Making a Film for Crowdfunding]]<br />
<br />
<br><br />
<br />
or follow our [https://calendar.google.com/calendar/embed?src=mgvq9goephpmna0kb7rj9ipei8%40group.calendar.google.com&ctz=Europe/Zurich google calendar]<br><br />
<br />
==Other Upcoming Events at [http://www.univercite.ch UniverCité]==<br />
<br><br />
<br />
==Other Upcoming Events==<br />
<br><br />
<br />
==Past Hackuarium Events==<br />
<br><br />
You can find the complete list of our '''past events''' [[past events | here]].<br />
<br />
<br><br />
<br />
=Projects=<br />
<br />
Here, you will find the projects going on in the Hackuarium.<br />
<br />
== Active Projects ==<br />
<br />
* [[Pushing_the_P1 | The P1 project]]<br />
* [[BeerDeCoded]]<br />
* [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Living Instruments]]<br />
* [[Micro to Macro Water Pollution]] - with [http://www.hammerdirt.ch/ Hammerdirt] and [http://biodesign.cc/ BIO-DESIGN for the REAL WORLD]<br />
* [[Spectro-pointer]]<br />
* [[Darty_Monkeys]]<br />
* [[Octanis]] - with more information about their Rover / Balloon [http://octanis.org/rover here]<br />
* [[Grow%26Mix_Bioink]]<br />
* [[unmapping]]<br />
* [[AGiR! for genomic integrity]]<br />
* [[Moss Menageries with AGiR!]]<br />
* [[Open_Source_Bioreactor | Open Source Bioreactor]]<br />
* [[Bioreactor | Steady state bioreactor]]<br />
* [[Workshop_Bioprinter| Workshop Bioprinter]]<br />
* [[Colab Biomaterials | Colab Biomaterials]]<br />
* [[HTGAA | HTGAA]]<br />
<br />
== Archived Projects ==<br />
<br />
* [[Hacktion | Hacktion challenges]]<br />
* [[Open-Food-DNA |Open-Food-DNA]]<br />
* [[Bience | Bience (Beer and Science!)]]<br />
* [[Mycelium Structures]]<br />
* [[Live Type]]<br />
* [[Projects:LivingLab Design|LivingLab Design]]<br />
* [[Projects:bioluminescence|Bioluminescence]]<br />
* [[Projects:Quantitative Anthropology|Quantitative Anthropology]]<br />
* [[Projects:Impression 3D|Impression 3D]]<br />
* [[Projects:Dépollution des stands de tirs Suisses]]<br />
* [[Incubateur bon marché]]<br />
* [[Space Seed]]<br />
* [[Reception d'images satellites]]<br />
* [[Edible wall]]<br />
* [[ZéBU CH]]<br />
* [[Compost Bacteria]]<br />
* [[Protein purification systems]]<br />
<br />
<br />
<br><br><br />
<br />
= Practical Information =<br />
<br />
Following this [[ Media:Univercite map2-01.png | link]] you will find a general plan of Hackuarium.<br><br />
Following this [[ Media:Map 2-01.png | link]], you will find the plan of Hackuarium with the position of equipment.<br />
<br />
== [[Life_Main | Rules at Hackuarium]] ==<br />
[[Life_Main | This page ]] describe the code of conduct for Hackuarium members.<br />
<br />
<br />
==[[Create an Event! | Create an Event!]]== <br />
<br />
Wiki express for EVENT organisation: <br />
<br><br />
*Want to organise an event with particulars/companies/universities/your grandpapa and mama???? <br />
Talk to Christophe Rouiller christophe@univercite.ch to check if the space is free <br />
AND<br />
Talk ALSO to Yann Pierson our 'online promotion' specialist, who will make sure the announcements are made on all the social media outlets for Hackuarium!<br />
Talk to the community, to spread the word (not for permission) —> slack UC Events (best) and/or board channel (cool), or also board@hackuarium.ch<br />
<br><br />
Go for it!<br />
<br><br />
*Is it a WORKSHOP?? All the previous & make sure you get your logistics in order! Do you need a microgrant? Just ask!!<br />
*Is there a SIMILAR workshop ALREADY planned? If yes, negotiate dates and: Do you target the SAME people? If yes, negotiate. If not, no problem. It maybe interesting to see what others do. <br />
*Go for it! and spread the love for tech, science, art and design. <br />
<br><br />
<br />
== [[Protocols | Protocols]] ==<br />
All the protocols that we use at Hackuarium will be found [[ BioP_Main | here ]] (work in progress).<br />
They are detailed and structured so that the main focus is on applications. The idea is to give crucial information on how to apply the most common techniques of the Biology toolbox. The protocols are structured as follow:<br />
::<li> A scheme describing each <br />
::<li> A brief description of the protocol and its use<br />
::<li> A description of the different ways of carrying out the protocol. Ideally there should be a description of a commercial kit and a DIY kit.<br />
::<li> A description of the safety issues and best practice<br />
::<li> Information specific to Hackuarium and where to find the components, tools and reagents.<br />
<br />
== [[Instru_Main | Instruments available]] ==<br />
[[Instru_Main | This page ]] describes all the instruments available at Hackuarium (work in progress..), their location and how to use them.<br />
<br />
== Up-to-date list of instruments we still need ==<br />
This [https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing on-line document] contains all the instruments, tools and consumables we are looking for. Our standard donation contract (French) [[Contrat_de_donation]]. If your organisation happen to discard a piece of equipment we are (or may be) looking for, [mailto:hello@hackuarium.ch contact us]!<br />
<br />
== [[Where we shop | Where we shop]] ==<br />
===Wetware===<br />
Consumables can be purchased from [http://www.huberlab.ch Huber Lab].<br />
<br />
Swiss-based [http://www.smiples.ch Smiples] has a very extensive listing of second hand laboratory and technical items. These can be bought from their online shop or from [https://www.ricardo.ch/online-shop/smiples/?SellerNickName=Smiples Ricardo].<br />
<br />
===Hardware===<br />
Options for electronic parts<br />
* [http://www.ebay.com Ebay] (international)<br />
* [http://www.aliexpress.com AliExpress] (China)<br />
* [https://www.adafruit.com Adafruit] (UK)<br />
* [http://www.seeedstudio.com/depot/ Seeedstudio] (China)<br />
* [http://www.tmart.com/ Tmart] (Hong Kong)<br />
* [https://www.pi-shop.ch/ Rasberry pi shop] (Switzerland)<br />
* [http://www.distrelec.ch/ Distrelec] (Switzerland)<br />
<br />
==How to built a new project page on our Wiki==<br />
<br />
If you want to start or edit a project page on Hackuarium's wiki, please follow these [[Editing the wiki | instructions]].<br />
<br><br />
<br />
==Library and ReadingList ==<br />
<br />
=== The DIY biology / Biohacking movement ===<br />
<br />
<br><br />
<br />
<li>[http://delfanti.org/biohackers/ Biohackers. The politics of open science] by Alessandro Delfanti (2013) London: Pluto Press.<br />
::[http://delfanti.org/wp-content/uploads/2013/05/Science-2014-Golinelli-521.pdf A review] by [[User:luchenry | Luc Henry]]<br />
<li>[http://link.springer.com/article/10.1186/s40504-016-0039-1 DIY-Bio – economic, epistemological and ethical implications and ambivalences] by Jozef Keulartz and Henk van den Belt (2016) Life Sciences, Society and Policy<br />
<li>[http://www.biobasedworldnews.com/biohacking-everything-you-need-to-know-about-diy-biology Biohacking] everything you need to know about diy biology, (Accessed 05.2016)<br><br />
<li>[http://www.prnewswire.com/news-releases/genspace-nyc-receives-350000-in-support-from-the-simons-foundation-300237457.html Genspace gets funding], PRNewswire, (Accessed 05.2016)<br><br />
<li>[http://www.nature.com/news/governance-learn-from-diy-biologists-1.19507 Learn from DIY biologists], Todd Kuiken, Nature, (Accessed 05.2016)<br />
<br />
<br><br />
<br><br />
<br />
And a much more complete list by [https://www.kuleuven.be/wieiswie/en/person/00105850 Massimiliano Simons], KU Leuven:<br />
<br />
<br><br />
<br><br />
<br />
<li>[http://www.the-scientist.com/?articles.view/articleNo/34457/title/The-Rebirth-of-DIYbio/ The Rebirth of DIYbio] by Jef Akst, The Scientist, March 2013<br />
<li>[https://www.newscientist.com/article/mg21829236-300-citizen-scientist-out-of-the-lab-and-onto-the-streets/ Out of the lab and onto the streets] by Kat Austen, New Scientist, June 2013<br />
<li>[https://academic.oup.com/bioscience/article/65/1/112/379448/DIYbio-Alternative-Career-Path-for-Biologists DIYbio - Alternative Career Path for Biologists?] by Beth Baker, BioScience (2015) 65 (1): 112<br />
<li>[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1109.html From synthetic biology to biohacking: are we prepared?] by Gaymon Bennett, Nils Gilman, Anthony Stavrianakis & Paul Rabinow, Nature Biotechnology 27, 1109 - 1111 (2009)<br />
<li>[http://repository.jmls.edu/ripl/vol10/iss3/1/Patent Office as Biosecurity Gatekeeper: Fosering Responsible Science and Building Public Trust in DIY Science] by Brian J. Gorman, J. Marshall Rev. Intell. Prop. L. 423 (2011)<br />
<li>[http://wi.mobilities.ca/roberta-buiani-biolab-on-wheels-finding-a-space-for-a-diy-bio-lab-in-toronto/ Biolab-on-Wheels: finding a space for a DIY bio lab in Toronto] by Roberta Buiani, Journal of Mobile Media, February 2015<br />
<li>Charisius, Hanno & Friebe, Richard & Karberg, Sascha - Biohacking: Gentechnik aus der Garage<br />
<li>Curry, Helen Anne - From garden biotech to garage biotech: amateur experimental biology in historical perspective<br />
<li>Davies, Sarah R. & Karin Tybjerg & Louise Whiteley & Thomas Söderqvist - Co-curation as hacking: biohackers in Copenhagen's Medical Museion<br />
<li>Delfanti, Alessandro - Biohackers: The Politics of Open Science<br />
<li>Delfanti, Alessandro - Tweaking genes in your garage: biohacking between activism and entrepreneurship<br />
<li>Delfanti, Alessandro - Is Do-it-Yourself Biology Being Co-opted by Institutions?<br />
<li>Delfanti, Alessandro - Distributed biotechnology<br />
<li>Delfanti, Alessandro - Hacking genomes. The ethics of open and rebel biology<br />
<li>Delgado, Ana - DIYbio: Making things and making futures<br />
<li>Editorial - Empowering citizen scientists<br />
<li>Eggleson, Kathleen - Transatlantic Divergences in Citizen Science Ethics - Comparative Analysis of the DIYbio Code of Ethics Drafts of 2011<br />
<li>Gewin, Virginia - Independent streak<br />
<li>Golinelli, Stefano & Guido Ruivenkamp - Do-it-yourself biology: Action research within the life sciences<br />
<li>Grushkin, D., Kuiken, T., Millet, P - Seven Myths & Realities about Do-It-Yourself Biology<br />
<li>Grushkin, Daniel - Am I a biohazard?<br />
<li>Holloway, Dustin - Regulating Amateurs<br />
<li>Jefferson, Chaterine - Governing Amateur Biology: Extending Respnonsible Research and Innovation in Synthetic Biology to New Actors<br />
<li>Kean, Sam - A Lab of Their Own<br />
<li>Kelty, Christopher - Outlaw, hackers, victorian amateurs: diagnosing publich participation in the life sciences today<br />
<li>Kera, Denisa - Hackerspaces and DIYbio in Asia: connecting science and community with open data, kits and protocols<br />
<li>Kera, Denisa - Innovation regimes based on collaborative and global tinkering: Synthetic biology and nanotechnology in the hackerspaces<br />
<li>Kuiken, Todd - DIYbio: Low Risk, High Potential<br />
<li>Kuiken, Todd - Learn from Do-It-Yourself Biologists<br />
<li>Kuznetsov, Stacey - Expanding Our Visions of Citizen Science<br />
<li>Kuznetsov, Stacey & Alex Taylor & Tim Regan & Nicolas Villar & Eric Paulos - At the seams: DIYbio and opportunities for HCI<br />
<li>Kuznetsov, Stacey & Carrie Doonan & Nathan Wilson & Swarna Mohan & Scott E. Hudson & Eric Paulson - DIYbio Things: Open Source Biology Tools as Platforms fo rHybrid Knowledge Production and Scientific Participation<br />
<li>Landrain, Thomas & Meyer, Morgan & Perez, Ariel Martin & Sussan, Remi - Do-it-yourself biology: challenges and promises for an open science and technology movement<br />
<li>Lisa Z. Scheifele & Thomas Burkett - The First Three Years of a Community Lab: Lessons Learned and Ways Forward<br />
<li>McKenna, Phil - Rise of the garage genome hackers<br />
<li>Meyer, Morgan - Build your own lab<br />
<li>Meyer, Morgan - Domesticating and democratizing science: a geography of do-it-yourself biology<br />
<li>Meyer, Morgan - Hacking Life? The Politics and Poetics of DIY Biology<br />
<li>Meyer, Morgan - Bricoler, domestiquer et contourner la science<br />
<li>Nascimento, Susana & Angela Guimaraes Pereira & Alessia Ghezzi - From Citizen Science to Do It Yourself Science<br />
<li>NSABB - Strategies to Educate Amateur Biologists and Scientists in Non-life Science Disciplines About Dual use Research in the Life Science<br />
<li>Schmidt, Markus - Diffusion of synthetic biology<br />
<li>Scudellari, Megan - Biology Hacklabs<br />
<li>Seyfried, Günter & Pei, Lei & Schmidt, Markus - European do-it-yourself (DIY) biology: Beyond the hope, hype and horror<br />
<li>Sholette, Gregory - Disciplining the avant-garde: The United States versus the Critical Art Ensemble<br />
<li>Sipra Bihani & Michael Hartman & Florian Sobiegalla & Amanda rosenberg - Comparing network strutures of commercial and non-commercial biohacking online-communities<br />
<li>Söderberg, Johan & Delfanti, Alessandro - Hacking Hacked! The Life Cycles of Digital Innovation<br />
<li>Söderberg, Johan & Delfanti, Alessandro - Repurposing the hacker. Three temporalities of recuperation<br />
<li>Tocchetti, Sara - DIYbiologists as 'Makers' of Personal Biologies<br />
<li>Tocchetti, Sara - What kind of work we are doing now and what kind of work we want to do<br />
<li>Tocchetti, Sara & Sara Angeli Aguiton - Is an FBI Agent an DIY Biologist Like Any Oter? A Cultural Analysis of a Biosecurity Risk<br />
<li>Trojok, Rüdiger - Biohacking: Gentechnologie für Alle<br />
<li>van Boheemen, Pieter & Huib de Vriend - Do-it-yourself biology: Een verkenning van ontwikkelingen in Nederland<br />
<li>Wohlsen, Marcus - Biopunk: Solving Biotech's Biggest Problems in Kitchens and Garages<br />
<br />
=== Open Hardware ===<br />
<li>[http://www.nature.com/news/open-hardware-pioneers-push-for-low-cost-lab-kit-1.19518 Open-hardware, pioneers push for low-cost lab kit]Elizabeth Gibney, Nature, (Accessed 05.2016)<br><br />
<li>[http://www.appropedia.org/Open-source_Lab#Examples Appropedia] a source of DIY instruments builds, Dr. Joshua Pearce, (Accessed 05.2016)<br><br />
<li>[http://www.ohwr.org/projects/fr_pcb_mm The PCB milling machine], Open Hardware Repository, (Accessed 05.2016)<br><br />
:: [https://pt.wikiversity.org/wiki/Pesquisa:Ferramentas_livres:Work_group_for_development_of_the_hyperobject_workbench More]<br />
<li>http://www.creativeapplications.net/processing/sensortape-3d-aware-dense-sensor-network-on-a-tape/, Sensory network on a roll of tape, Filip Visnjic, (Accessed 05.2016)<br><br />
<li>[http://oceanoptics.com/product/redeye-oxygen-sensing-patches/ RedEye Oxygen Sensing Patches], Ocean Optics, (Accessed 05.2016) <br><br />
<br><br />
<br />
=== Art ===<br />
<li> [http://www.booooooom.com/2016/04/04/mattia-menchetti-gives-wasps-coloured-paper-to-create-rainbow-nests/#more-78906 Rainbow wasp nests], Mattia Menchetti, (Accessed 05.2016)<br><br />
<li> [http://thecreatorsproject.vice.com/blog/dirty-beats-hear-music-generated-from-bacteria Dirty beats] making music with bacteria, Interspecifics(Accessed 05.2016)<br><br />
::[http://www.interspecifics.cc/-/ More1], [http://www.ncbi.nlm.nih.gov/pubmed/21239171, More2], [http://www.ncbi.nlm.nih.gov/pubmed/12501293, More3]<br><br />
<br><br />
<br />
=== Ideas/Concepts/Science ===<br />
<li>[http://ideas.ted.com/a-newly-drawn-tree-of-life-reminds-us-to-question-what-we-know/ The new tree of life] TED (Accessed 05.2016)<br><br />
<li>[https://medium.com/now-of-work/the-workplace-of-the-future-work-of-today-16c58df1fa29#.zhbu0c57f Rethinking work], Yann Heurtaux, (Accessed 05.2016)<br><br />
<li>[https://www.kickstarter.com/projects/910418035/plastic-bottle-cutter That plastic bottle cutter], Pavel & Ian, (Accessed 05.2016)<br><br />
<br><br />
<br />
=== Open Source ===<br />
<li>[https://blogs.openaire.eu/?p=882 Results held hostage], Francesco Mondada, (Accessed 05.2016)<br><br />
<br><br />
<br />
==Visual Identity==<br />
* [[Logo]]<br />
* [http://wiki.hackuarium.ch/images/9/9d/HackuariumPoster.pdf Our poster in pdf ready to print]<br />
* Working on a footage for videos (comming soon)<br />
<br />
== Media and Press ==<br />
<br />
* 2016.09.27 - Hackuarium is mentioned in a paper by Celia Luterbacher on citizen science: [http://www.swissinfo.ch/eng/democratisation_citizen-science--not-a-scientist--not-a-problem/42437940 Citizen science: Not a scientist? Not a problem]<br />
<br />
*2016.09.18 - [[User:Luchenry | Luc Henry]], co-founder of Hackuarium, is interviewed in this podcast on citizen science: [http://www.swissinfo.ch/eng/podcast_science-for-non-scientists/42438132 Science for non-scientists]<br />
<br />
*<br />
<br />
== Wanted ==<br />
Do you want to contribute to making Hackuarium a diverse and lively community? Check out our wanted lists:<br />
::<li>Skills<br />
::<li>Ideas<br />
::<li>[https://docs.google.com/spreadsheets/d/1agHVlyMzcg_cdjTlg8bABzjHnOc7Gu0B8v2FGhcEabU/edit?usp=sharing Equipment]<br />
::<li>Consumables<br />
<br />
<br><br></div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=9568Pushing the P12017-09-18T14:00:26Z<p>Dan.fhg: /* Material */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
<br />
Original Timeline had the following steps: We probably have to start again with the HTGAA course starting in Fall 2017.<br />
<br />
mise en place équipe travail + évaluation des points critiques (cout, autorisations) 1 mois<br />
<br />
Mise en place de laboratoire + obtention du materiel 1 mois<br />
<br />
discussion avec les autoriteés et obtention des autorisations 2 semaines<br />
<br />
<br />
Original schedule: could not be held due to renovations in the building<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
Material to be used for HTGAA 2017 is here:<br />
https://docs.google.com/spreadsheets/d/1Xg_GtrFb8wYJVUPlrbUb6pP0dWGmkoAKU4xP6qhBKVE/edit#gid=0<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html<br />
to find the official Swiss classification.<br />
<br />
The second step is to borrow the microbe from someone that already used it before. All BSL1 manipulations are announced to the government that makes the database freely accessible here: http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx go and find a researcher. Talking with someone that has experience on that specific microbe will be useful also to get some tips and tricks on growing conditions, etc..<br />
<br />
If nobody worked with the microbe before. It is possible to purchase a culture from the Culture Collection of Switzerland (CH): https://www.ccos.ch/<br />
<br />
Additional repositories:<br />
*Algae and protozoa (UK): https://www.ccap.ac.uk/<br />
*Eukariotic cells and microbes (US):ATCC https://www.lgcstandards-atcc.org/?geo_country=ch Note: the ATCC BSL classification is American. It may differ from the Swiss one. Check the Swiss one before!<br />
*Czech Collection of Microorganisms (CCM) http://www.sci.muni.cz/ccm/index.html<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Backup of the first exploration document : http://wiki.hackuarium.ch/w/Talk:Pushing_the_P1#Old_Hackpad_Page (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
*6. Questions? There is a service in the DIYBio community for [http://ask.diybio.org/ Ask a Biosafety Professional]<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=9567Pushing the P12017-09-18T13:42:43Z<p>Dan.fhg: /* Timeline */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
<br />
Original Timeline had the following steps: We probably have to start again with the HTGAA course starting in Fall 2017.<br />
<br />
mise en place équipe travail + évaluation des points critiques (cout, autorisations) 1 mois<br />
<br />
Mise en place de laboratoire + obtention du materiel 1 mois<br />
<br />
discussion avec les autoriteés et obtention des autorisations 2 semaines<br />
<br />
<br />
Original schedule: could not be held due to renovations in the building<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html<br />
to find the official Swiss classification.<br />
<br />
The second step is to borrow the microbe from someone that already used it before. All BSL1 manipulations are announced to the government that makes the database freely accessible here: http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx go and find a researcher. Talking with someone that has experience on that specific microbe will be useful also to get some tips and tricks on growing conditions, etc..<br />
<br />
If nobody worked with the microbe before. It is possible to purchase a culture from the Culture Collection of Switzerland (CH): https://www.ccos.ch/<br />
<br />
Additional repositories:<br />
*Algae and protozoa (UK): https://www.ccap.ac.uk/<br />
*Eukariotic cells and microbes (US):ATCC https://www.lgcstandards-atcc.org/?geo_country=ch Note: the ATCC BSL classification is American. It may differ from the Swiss one. Check the Swiss one before!<br />
*Czech Collection of Microorganisms (CCM) http://www.sci.muni.cz/ccm/index.html<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Backup of the first exploration document : http://wiki.hackuarium.ch/w/Talk:Pushing_the_P1#Old_Hackpad_Page (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
*6. Questions? There is a service in the DIYBio community for [http://ask.diybio.org/ Ask a Biosafety Professional]<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Pushing_the_P1&diff=9566Pushing the P12017-09-18T13:41:59Z<p>Dan.fhg: /* Timeline */</p>
<hr />
<div>=Introduction=<br />
Having a laboratory and instruments is one thing, possessing the tools to do actual biology research is another thing. For close to two years now, the Hackuarium community has built a space, where people can work on projects related to biology among other fields. The ambition of the international DIY biology scene is to bring to the people the tools to perform and work on the whole range of biological applications, as qualitatively and cheaper than what the industrial or academic institutions do. To achieve such level, the Hackuarium lab has to be upgraded to a higher level of competency. One aspect is the P1 biosafety level where genetic manipulation opens the door to a vast range of research topic and engineering opportunities. This scientifically enriching, yet delicate step, is now in motion but in order to start practicing several items involving legal, technical and community topics have to be settled. Indeed, our laboratory practices and transparency as a citizen lab have to be absolutely irreproachable. The challenges we face are described in this document.<br><br><br />
We will prepare for the P1 work assuming it will start out in bacteria, and then depending on future demands, we imagine adding other organisms.<br><br><br />
[[File:Map 33.png|600px|thumb|center|The future lab configuration]]<br />
= Abreviations =<br />
Biological safety level 1 lab (P1), biosafety officer (BSO), standard operating procedure (SOP),<br />
<br />
=Timeline=<br />
<br />
Original Timeline had the following steps: We probably have to start again with the HTGAA course starting in Fall 2017.<br />
<br />
mise en place équipe travail + évaluation des points critiques (cout, autorisations) 1 mois<br />
<br />
Mise en place de laboratoire + obtention du materiel 1 mois<br />
<br />
discussion avec les autoriteés et obtention des autorisations 2 semaines<br />
<br />
<br />
Here is the basic timeline we will try to keep in order to make the project move on. It is obviously a "best case scenario" approximation.<br />
[[File:P1_timeline.PNG|1000px|thumb|center|Proposed timeline for the project]]<br><br><br />
The schedule could not be held due to renovations in the building<br />
<br />
=Critical Points=<br />
'''What we need to sort out before we start:'''<br />
<br />
'''Announce the first activities''' <br><br />
::YES, Luc has a form<br />
'''Will the enclosure be satisfactory?'''<br />
::YES, but it may be a good idea to inform the Canton <br />
'''Do we need a bio safety officer? Does this person need a specific training?'''<br />
::Legally yes! I think it is good if 2-3 people get this training, either at EPFL, or in Bern.<br />
'''Risk assessment'''<br />
::*The biological risk assessment will be made through the form each member as to fill when he creates a new genetically modified strain to ensure no pathological or harmful gene is inserted in the micro-organism. <br />
::*Chemically, any inflammable liquid will be stored in the solvent cabinet. The acids and bases will be separated in two "geographically" remote cabinets. The bottles will be stored close to ground level in a resistant container able to contain the full volume of the bottles it contains to avoid spillage. <br />
'''Waste elimination'''<br />
:: -> OK (see below)<br />
<br />
Federal Coordination Centre for Biotechnology (where you have to notify level 1 activities with GMOs):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=en<br><br />
<br />
BSO-Curriculum (courses for biosafety officers):<br><br />
http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=en<br><br />
<br />
Cantonal authority (Vaud, contact persons)<br><br />
-Isabelle Dessaux (isabelle.dessaux@vd.ch )<br><br />
-Olivier Gianina (olivier.gianina@vd.ch )<br><br />
<br />
'''Our (very helpful) contact is:'''<br><br />
<br />
Manuela Ocaña<br><br />
Collaboratrice scientifique, PhD<br><br />
<br />
Département fédéral de l'intérieur DFI<br><br />
Office fédéral de la santé publique OFSP<br><br />
Unité de direction Santé publique<br><br />
<br />
Schwarzenburgstrasse 157, 3003 Berne<br><br />
Tél. +41 58 462 63 66<br><br />
Fax +41 58 462 62 33<br><br />
manuela.ocana@bag.admin.ch<br><br />
www.bag.admin.ch<br><br />
<br />
'''Her initial email was as follow:'''<br><br />
<br />
Bonjour,<br><br />
<br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br><br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du [http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr Bureau de Biotechnologie de la Confédération] puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br><br />
<br />
N’hésitez à me contacter en tout temps si vous avez des questions ou besoin d’informations supplémentaires.<br />
<br />
<br><br />
<br />
=Pivot Points=<br />
Here we develop and tackle down the most critical points of this project. The idea is to estimate if our space can accommodate such an infrastructure and if our community has the shoulders to carry the necessary responsibilities. The points have to be assessed keeping in mind the first standardized lab procedure that will be globally used by the lab (see generalized procedure). <br />
<br />
==Lab room and material==<br />
Here we discuss the size features and needed characteristics of the room we are going to use for the lab. We also describe a material list we would need to equip the lab to the minimum. <br />
<br />
===Room features===<br />
<br />
===Material===<br />
<br />
All the details for the material we would need is on the following doc: <br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=1606970814<br />
<br />
==Waste eliminations==<br />
Here we describe who can and accepts to eliminate our wastes at what cost and try to estimate the frequency of elimination.<br />
We base the frequency on a per project base. How many liters of cultures are produced per production? How many biowaste bag can be filled in a week? etc...<br />
===Procedure===<br />
Based on: http://www.cusstr.ch/repository/138.pdf<br />
*'''Les souches non pathogènes et non modifiées génétiquement ne nécessitent pas d’inactivation.''' Les déchets liquides peuvent être éliminés dans l’évier pour autant qu’il n’y ait pas d’autres contaminants p.ex. chimiques ou radioactifs. Les déchets solides doivent être éliminés avec les déchets incinérables, avec les mêmes restrictions que les liquides.<br />
*'''Tout matériel de laboratoire contaminé par un OGM de classe 1 doit être inactivé''' avant élimination pour une '''filière de déchet normal'''. Un marquage clair des sacs à déchets biologiques doit être effectué afin de pouvoir identifier aisément les déchets autoclavés de ceux qui ne l’ont pas encore été. Par exemple, le sac auroclavé sera transféré dans un autre sac de couleur différente ne laissant plus apparaître le sigle biohazard et le contenu.<br />
*Les objets tranchants ou coupants doivent être éliminés comme déchets spéciaux.<br />
''' In english in a sentence '''<br />
Bio-waste bags have to be clearly distinguishable from other waste bags for example with the bio-hazard sign on them. Once inactivated (i.e. autoclaved) you have to package the waste in a way that it neither doesn't look nor doesn't smell disgusting or dangerous. Then you can discard it as household waste. If this is not possible, it needs to be discarded as special waste, how it is done at EPFL (white bag with red stripes).<br />
<br />
===Service providers===<br />
Since the bags of solid wastes can be eliminated through the classical way and the autoclaved liquids can be poured to the toilets the cost can be estimated negligible.<br><br />
No need for special waste removal<br><br />
<br />
==Calculated base costs==<br />
*Here we estimate the cost of starting the whole setup. <br />
*We take a standardized method which is documented lower (protocol) based only on biology with e.coli.<br />
*The costs are detailed on the doc below and is calculated on the academic standards.<br />
*The goal then is to find hacks and shortcuts to largely cut the costs and make it affordable.<br />
https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0<br />
<br />
==Softwares and Databases==<br />
Here we discuss the gene editing softwares to design plasmids, primers and gene building blocks. A total transparency has to be implemented in order for people to follow our activities. What kind of database will we use to store the ordered primers sequences? Same for the plasmids? <br />
<br />
===Softwares===<br />
*[http://www.geneious.com Geneious] powerful but the file format is not opensource or genebank<br />
*[http://www.snapgene.com/products/snapgene_viewer/ Snapgene] never tried, anyone ?<br />
<br />
===Database===<br />
<br />
==Providers==<br />
Here we discuss the possible service providers, their costs and if they are ready to deal with us. We canot create plasmids without primers or genes and therefore need providers. We cannot confirm the authenticity of our work without a verified plasmid sequence and therefore need sequencing services.<br />
<br />
===Microbes===<br />
We have only the right to work with organisms classified as BSL1 according to Swiss Law.<br />
<br />
The first step is to check if the microbe you want to work is BSL1, go to https://www.bafu.admin.ch/bafu/fr/home/themes/biotechnologie/publications-etudes/publications/classification-des-organismes.html<br />
to find the official Swiss classification.<br />
<br />
The second step is to borrow the microbe from someone that already used it before. All BSL1 manipulations are announced to the government that makes the database freely accessible here: http://www.ecogen.ch/ecogen/Forms/Register/RegisterSearch.aspx go and find a researcher. Talking with someone that has experience on that specific microbe will be useful also to get some tips and tricks on growing conditions, etc..<br />
<br />
If nobody worked with the microbe before. It is possible to purchase a culture from the Culture Collection of Switzerland (CH): https://www.ccos.ch/<br />
<br />
Additional repositories:<br />
*Algae and protozoa (UK): https://www.ccap.ac.uk/<br />
*Eukariotic cells and microbes (US):ATCC https://www.lgcstandards-atcc.org/?geo_country=ch Note: the ATCC BSL classification is American. It may differ from the Swiss one. Check the Swiss one before!<br />
*Czech Collection of Microorganisms (CCM) http://www.sci.muni.cz/ccm/index.html<br />
<br />
===Primers===<br />
* [http://www.microsynth.ch/ Microsynth] : ~ 10.-/30 bp primer<br />
<br />
===Sequencing===<br />
*[http://www.gatc-biotech.com/fr/lp/sequencage-nextgen.html?gclid=CKzRrtTbm80CFdiZGwodBycOaA GATC]<br />
<br />
===Genes===<br />
<br />
==Legal==<br />
Backup of the first exploration document : http://wiki.hackuarium.ch/w/Talk:Pushing_the_P1#Old_Hackpad_Page (Most of the necessary infos can be found here)<br><br />
As said previously our laboratory practices have to be irreproachable. We carry the "hacker" label and therefore, for the public opinion and the built of trust, cannot fuck around. We need to study in depth several aspects of the legislation governing genetic manipulation. <br />
===WHO===<br />
The very good lab safety manual right [http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/ here]<br><br />
Go through it if you have the time or are curious, safety in the lab is everybody's job.<br><br />
<br />
===Swiss legislation===<br />
On Feb 3 2016, we got some pointers from the Vice Dean of the School of Biology of UniL who passed us information from Audvion.ch (Ingénieur de sécurité MSST)<br />
<blockquote><br />
From:<br />
Objet: AVP réponse biosécurité Re: Laboratoires P1<br />
Date: 3 février 2016 13:58:56 UTC+1<br />
<br />
Pour toutes les questions de sécurité biologique (biosécurité) les règles de base sont données dans deux ordonnances doit :<br />
*OUC Ordonnance sur l'utilisation des organismes en milieu confiné https://www.admin.ch/opc/fr/classified-compilation/20100803/index.html<br />
*OPTM Ordonnance sur la protection des travailleurs contre les risques liés aux microorganismes https://www.admin.ch/opc/fr/classified-compilation/19994946/index.html<br />
<br />
Vous pouvez aussi consulter<br />
*Le site de la CUSSTR (Commission universitaire pour la santé et la sécurité au travail romande) http://cusstr.ch/fr/doc/technique/detail/?idcat=14<br />
*Un petit aide mémoire de la SUVA sur le sujet (missing link)<br />
*Vous référer au coordinateur de biosécurité BSO de votre département<br />
</blockquote><br />
<br />
On Feb 25 2016, we got some more pointers from the Office fédéral de la santé publique OFSP<br />
<blockquote><br />
L’office fédéral de la santé publique (OFSP) est, en collaboration avec l’office fédéral de l’environnement (OFEV), responsable pour les aspects de sécurité biologique en Suisse. L'ordonnance sur l'utilisation des organismes en milieu confiné (ordonnance sur l’utilisation confinée, OUC ; RS 814.912) règle l'utilisation d'organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné (par ex. laboratoires de recherche, de diagnostic ou d’enseignement et développement). Cette ordonnance a pour but de protéger l’être humain, les animaux et l'environnement des menaces et atteintes possibles résultant de l’utilisation en milieu confiné de tels organismes. Les instituts, les entreprises et les organisations qui utilisent des organismes génétiquement modifiés, pathogènes ou exotiques en milieu confiné sont ainsi tenus de notifier leur activité (classes 1 et 2) ou de demander une autorisation (classes 3 et 4).<br />
<br />
Nous avons pris connaissance, avec grand intérêt, des informations concernant les activités et prestations proposées par votre laboratoire « Hackuarium », qui pourraient par ailleurs tomber dans le champ d’application de l’OUC. En effet, et selon le type de matériel utilisé (par ex. organismes génétiquement modifiés ou pathogènes) ainsi que la nature et l’ampleur de vos activités, ces dernières pourraient être soumises au devoir de notifier selon l’OUC. Mais avant d’entreprendre des démarches qui pourraient s’avérer finalement inutiles, je vous propose de consulter d’abord les informations disponibles sur le site du Bureau de Biotechnologie de la Confédération (http://www.bafu.admin.ch/biotechnologie/01744/01745/index.html?lang=fr ) puis de reprendre contact avec nos services afin de clarifier au mieux votre situation.<br />
</blockquote><br />
<br />
===BioSafety Officier (BSO)===<br />
Courses : http://www.bafu.admin.ch/biotechnologie/01744/02964/index.html?lang=f<br />
Infos:<br />
BSO, niveau de sécurité 1 (BSL1_2016)<br><br />
Date: 9 septembre 2016 (1 jour)<br><br />
Lieu: Université de Berne<br><br />
Inscription d'ici au 30 juin 2016 à: registration@curriculum-biosafety.ch<br><br />
Coûts: 550 francs par personne<br><br />
Langue: anglais<br><br />
<br />
===Annonce confédération===<br />
Through the [http://www.bafu.admin.ch/biotechnologie/01744/03225/index.html?lang=fr BAFU portail]<br />
<br />
===Norms===<br />
<br><br><br />
<br />
=Generalized procedure=<br />
These procedure will be implemented in the lab as first benchmark in order to have a work basis. <br><br />
The "project request" procedure will be a way to ensure complete transparency and applicability. It will have to be filled before any project can start by any member willing to perform GMO activities. <br><br />
<br />
They are optimized for cost & material efficiency. <br />
==Maximal Culture Size==<br />
Technically the space does not allow us yet to use flasks over a volume of 500ml (incubator volume). A culture grows well at a fifth of the total volume (oxigenation requisit) which theoretically makes it 100ml culture. Safety wise, using a rather small enclosed space, spills have to stay managable. To avoid bacterial spread outside of the enclosure a maximal volume of populated medium of 200ml per flask is allowed. A maximal volume of 1L in total, populated or sterile medium, is allowed.<br />
<br />
==Spill management==<br />
Droping a vial containing liquid is common. In a lab if not prpoerly handled a spill can have more dramatic effects than droping your milk at home. Here we depict the SOP for the 4 types of spills that happen with their respective anti-spill kits; acidic, basic, contaminated and neutral spills.<br />
<br />
*1. Alert your collaborators<br />
*2. Unplug and turn-off all proximate electric equipment. <br />
*3. Follow the procedure describing your situaion:<br />
:: <br />
'''Acidic spill'''<br />
<br />
==Project Request==<br />
Develop a document/wiki page as template where people will have to explain the GMO they want to produce. <br><br />
As an idea it could be a form or a wiki page that '''anybody''' could see structured as the following<br />
*Goal:<br />
:: - What is the purpose of the GMO / protein you want to create OR: What question should it help you answer<br />
::: ex: - Sense endocrine disruptors / Produce methane / Produce dyes<br />
*How does it help reach your goal<br />
::: ex: - This enzyme is known to biochemically produce red dye (add source)<br />
::: ex: - The combination of protein A,B&C could produce a sensor...<br />
*Plasmid<br />
:: - Tell which plasmid you will use (for archive purpose)<br />
:: - Give the code of the cassette you will use<br />
:: - Give the peptide translation of such cassette<br />
*Template<br />
:: - If you want to use several building blocks for you proteins, give details on the templates<br />
*Primers<br />
:: - Give the primers you are going to use to realise your clones<br />
<br />
== Storage and Nomenclature ==<br />
A recurrent behavior, often observed in institutionalized spaces, where the followup on ones item naming and storage procedure is inexistant, is the massive loss of time to find a special item belonging to a past member. Here we describe a strict naming procedure for items that is, we think, unambiguous and still logical.<br />
=== Users ===<br />
Oppositely to the Hackuarium's "P0" lab where users can come and experiment in the greatest of liberties the P1, as previously stated, will be restricted to card holders having followed an initiatory course. Each user will be registered in a database and will chose a three letter OR digit code to identify items made by himself. Example:<br />
* John Doe : joh<br />
* Martin Luther King : mlk<br />
* George Junior : gj2 <br />
This three letter acronym is practical as it is short enough to be stuck on small tubes with a descriptor i.e: joh_p7 (John Doe Plasmid 7)<br />
=== Descriptors ===<br />
In the process of producing for example a protein from scratch (from synthetic DNA) only a few items have to be stored and conserved for further use. These items often in the forms of solutions rarely exceed volumes of 200ul. Therefore the whole process of protein engineering and plasmid creation can hold in 1.5ml tubes stored in 96well cardboard freezer boxes. We list what we extimate should be conserved for further uses by other collaborators and give them a single letter code.<br />
* project/task/job : j<br />
* DNA segments : d<br />
* primers/amorces : a<br />
* plasmids : p<br />
* proteins/constructs : c<br />
* bacteria/strain : b <br />
These six items are the valuable items to be stored. The project (j) item might seem counter intuitive but makes sense in the whole nomenclature system. Let's give some examples with the hypothetical John Doe project 41 (i.e joh_j41).<br />
*He might do a plasmid (joh_j41_p1) that sadly he failed so he produced four more for sequencing (joh_j41_p2, joh_j41_p3, joh_j41_p4, joh_j41_p5).<br />
*The plasmid was a construction of a vector (joh_j41_dV) and two inserts (joh_j41_dI1, joh_j41_dI2)<br />
*The latter were made with six primers (joh_j41_a1, joh_j41_a2, joh_j41_a3, joh_j41_a4, joh_j41_a5, joh_j41_a6)<br />
*He finally made it and expressed his protein (joh_j41_c1) (Usually you only make a protein from a plasmid so if you re-express, as a 3rd batch, it later on: joh_j41_c3)<br />
The nomenclature might appear cumbersome at some point but it should make more sense once the final ''project draft'' is presented. <br />
<br />
=== Storage ===<br />
When you're in the working process, the following behavior is observed: You open the freezer, look for the box containing the items of interest (generally you have a primer box, a plasmid box, etc.), open the box and in those 96 tubes you want to find them by looking at the top of them. The "morphology" of a 1.5ml tube gives you only two options: You can write on the top only limited information. Forget about markers they will fade away unless you add transparent tape on top (which is boring). Or you can add stickers on the side of the tube that can contain more details on the content. So the following storage procedure will be observed:<br><br />
*Boxes will only contain a single type of items such as primers, plasmids or proteins. <br><br />
*Each item of whatever sort will have a unique 4 letter/digit hex code preceded by the type of item. That's roughly 65500 possibilities. This code is preceded by the adequate descriptor. Example: joh_j41_p1 (the plasmid N°1 of John's project 41) was given '''by the system''' the code p019A this is 5 letters which perfectly fit on a 1.5ml tube top if printed on a sticker. Again this looks cumbersome know but will make more sense later since the system gives and stores all the info for you.<br />
<br />
=== Job page === <br />
We presented previously the request form. This form generates for you a wiki project page or whatever online lab-book that we still have to find. The final idea is that on the server the access to these code and data is easily accessible from a terminal in the P1 lab. <br />
Here is an hypothetical [[hypo_proj | '''project job page''']] and how it should look like in the end '''after the form has been filled'''.<br><br />
When somebody works and experimentally gets the product design then he can directly fill the page or the online lab-book that should transfer the data.<br />
A final page, with experimental data filled in should look [[hypo_proj_pract | '''like this''']] <br><br />
If a page is filled with such details, reproducibility and experimental follow-up is much easier. The important point is having a strong automation of the main points so as to avoid time loss of creating manually those files (took me more than 30 min making a complete one by hand, it should not take more than 10 and should be done while doing the experiment). <br><br />
[http://wiki.hackuarium.ch/w/Form:GMO_Project » Project request form with above points is now here].<br />
<br />
==Bacterial==<br />
All of these components have to be open source.<br><br />
'''Strain''': e.coli<br><br />
'''Plasmid''': <br><br />
'''Resistance''': Ampicilin<br><br />
<br />
===DNA Amplification / Cloning===<br />
*Good old openTaq<br />
*DNA fragment purification by gel extraction. (cheap, easy, no need of DPNI enzymes)<br />
*Ligation through modified Gibson (lysed bacterial supernatant + enzymatic complementation (Exonuclease mostly), To investigate further see the [[Media:SLiCE.PDF | SLiCE]] method)<br />
*Transformation in chemo competent cells by heat shock (way cheaper than electro-competent cells)<br />
*Usual LB-agar plating with ampicilin selection<br />
*How do we purify the DNA from the cultures ? (yeah of course we can use qiagen minipreps but it's expensive...) There is an easy spermidine DNA prep that works all the times and costs nothing.<br />
<br />
===Primers production===<br />
Can we inspire ourselves from the cheap DIY peptide synthetiser and turn it to a primer systhetiser ? <br />
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045704/#!po=52.8409<br />
<br />
===Cultures===<br />
*e.coli chemo competent cells will be stored in a -80°C fridge. <br />
*Transformation will be made by heat shock as described in ("doc name here"). <br />
*Classical LB-Amp plating with static overnight 37°C incubation. <br />
*Liquid cultures can be either made as:<br />
::*LB-Amp followed by IPTG induction.<br />
::(-) IPTG is expensive<br />
::(-) Monitoring of optical density requires time + UV-spectro <br />
::(-) Large volumes (1L medium in 5L flasks) for decent yields + large capacity incubator cooling to 16°C = more waste<br />
::(-) Have to centrifuge in 500ml flasks = more post-culture work.<br />
::(+) yields are often good, procedure is bulletproof<br><br />
<br />
::*Modified auto-inducible Terrific-Broth (TB-of-doom)<br />
::(-) more complex medium preparation (can still be made as large stock)<br />
::(+/-) yields are good but proteins might be hard to extract<br />
::(+) No UV monitoring ("by eye check"), after growth expression can be made at room temp.<br />
::(+) small 100ml cultures in 500ml flasks<br />
::(+) all post-culture fork can be made in 2x50 ml flasks (convenient)<br><br />
<br />
=== Protein purification===<br />
*Purification can be made by single His tag purification<br />
::*We have more than 200ml of Ni-NTA resin<br />
::*The resin can be recycled easily<br />
::*Quite simple procedure (requires 3 buffers)<br />
::(-) Single His-tag purif will not yield absolutely pure proteins<br />
<br />
<br />
*Concentration<br />
::problem remains to be solved<br />
<br />
*Storage will be done at -20°C in 40% glycerol<br />
<br />
==Yeast==<br />
Yeast specialists please provide a protocol applicable here, keep in mind cost & material optimization.<br />
<br />
=OP Greenhouse Effect=<br />
Building the P1 in a green house... I can already smell ideas fresh as spring flourishing in there...<br />
<br />
==Idea==<br />
This clever and original idea was brought to us by our master of "let's just do it it's gonna work anyways" Gustavo, congratulation to him!<br><br />
Therefore we can buy a greenhouse for a reasonable price and set it up as a P1. The size will be indeed smaller but will be enough to accomodate the transformation, growth, cell lysis and waste storage until elimination.<br><br />
Several greenhouses models are presented with a rationale on which one to pick. <br><br />
The end of the document is supplemented with tasks and points to investigate <br><br />
<br />
==Greenhouses==<br />
We indeed aim for the best size/price ratio.<br><br />
*Serre Delia, Hornbach, 11m2, 1399.- [https://www.hornbach.ch/shop/Serre-Delia-6-Allin-256x434-cm-aluminium/5624313/article.html link]<br />
:: Initial idea<br />
:: (-) Expensive<br />
:: (+) Higher at the wall/roof junction (1m40) than the others. Still 1m40 is not very high. <br><br />
*Serre Vida XL, 12.25m2, VidaXL, 399.- [https://fr.vidaxl.ch/p/40193/serre-1225-m-alu-polycarbonate link]<br />
:: (+)Free delivery<br />
:: (+)Cheap<br />
:: (-)Low at the wall/roof junction (95cm). BUT! For the price we can get 2 and add the two walls on top of the other giving 1.9m!. <br><br />
<br />
==Building Plan==<br />
OBSOLETE: will be built where the storage is drawn<br />
[[File:GH_lab.png|500px|thumb|center|Greenhouse on top]] <br><br />
We can build the greenhouse in the corner there for the following reasons:<br />
*1. We can fix it to two walls<br />
*2. Add benches in front for people working outside to see what is inside (+ safety)<br />
*3. The benches can also ensure things are not going to be leaning against the walls<br />
<br />
==Tasks==<br />
===General===<br />
*1. Members validate the idea or propose other ideas until Wed. (DONE)<br />
*2. Ordering and building all together on the 2nd of Oct. (MATERIAL RECEIVED)<br />
*3. Building zone cleaned (DONE)<br />
<br />
===Material & Orders===<br />
*1. Rearrange lab space (DONE)<br />
*2. Think about a list of vital chems and consumables that are needed and could be stored in the P1 for easier access.<br />
:: look at: https://docs.google.com/spreadsheets/d/1tBvxEk4UVtvzfeW_-yR9yNbrLmC1V9IDjAPit37TzAg/edit#gid=0 and select the vital items.<br />
*3. Get labcoats of various sizes, get hooks for those<br />
*4. Get a set of safety goggles<br />
*5. Find fridge + -20 freezer<br />
*6. Order desinfectant dispenser<br />
*7. Get 3x6 multiplugs<br />
*8. Card access system ( can use yann's raspi3)<br />
*9. Get sturdy, closable bin for solid biowaste<br />
*10. Bin for classic wastes<br />
*11. Sam, get a hell stock of printable stickers (we're gonna label the shit out of everything)<br />
*12. Organize one cupboard with:<br />
:: pcr purif kits<br />
:: miniprep kits<br />
:: gel purif kits<br />
:: 50, 12 & 1.5 ml plastic tubes<br />
:: 1, 200 & 1000ul tips<br />
:: his tag purification buffers<br />
*13. On the sterile hood, find a plexiglass that we can fix on and that will cover 2/3 of the front pannel (i.e. Will make a separation betwwen the user and the working environement<br />
*13. Order a pipetor on SMIPLES / ricardo for serological pipettes<br />
:: https://www.fr.ricardo.ch/acheter/bureau-et-commerces/autres-metiers/laboratoires/pipette-17/v/an820658344/<br />
*14. We have two benches with the glass tops: one is not held with screws, fix it, buy long enough screws (obi)<br />
*15. Clean a set of micropipettes<br />
<br />
===Safety===<br />
*1. Announce our first activity on the confederation website (Yann)<br />
*2. Design the safety standard operating procedures (SOP) such as:<br />
:: MSDS for common chemicals<br />
:: What to do if a spillage of contaminated liquid occurs (how to clean it).<br />
:: What to do if you get injured working with bacteria.<br />
:: How to eliminate wastes, liquid and solid.<br />
:: Standard lab behavior (blouse, glasses, gloves)<br />
:: Where to store: acids, bases, solvents -> Rules for using each of them (how bases and acids have to be separated separately)<br />
:: A very good example : http://ehs.berkeley.edu/standard-operating-procedures<br />
*3. Print the following decals<br />
:: Biohazard 2x<br />
:: Authorized personnel only<br />
:: "Wash your hands when done" <br />
:: "Max X users inside" (X to be defined)<br />
*4. Find an easy an accessible way to put blouses and glasses (gloves can be stored and boxes left above the bench)<br />
*5. Make instrument procedures (print and put in a folder)<br />
*6. Questions? There is a service in the DIYBio community for [http://ask.diybio.org/ Ask a Biosafety Professional]<br />
<br />
===Ideas & Concerns===<br />
*As the name says it's a greenhouse, how do we make sure it doesn't become super warm ? Can we make a cheap air cycling system ?<br />
<br />
==Lab Setup==<br />
An idea: Can be discussed<br />
[[File:GH P1.PNG|300px|thumb|center|the essential in 12m2]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Bioreactor&diff=9559Bioreactor2017-09-15T10:53:24Z<p>Dan.fhg: </p>
<hr />
<div>This is a project whose immediate goal to set up a running chemostat. Once set up, there may be attempts to apply selective pressures on the population for biotechnological reasons.<br />
<br />
For more information contact [[User:Dan | Daniel Hernandez]]<br />
<br />
[[Category:Work In Progress]]<br />
[[Category:Projects]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=Bioreactor&diff=9558Bioreactor2017-09-15T10:52:25Z<p>Dan.fhg: </p>
<hr />
<div>This is a project whose immediate goal to set up a running chemostat. Once set up, there may be attempts to apply selective pressures on the population for biotechnological reasons.<br />
<br />
For more information contact [Dan Hernandez]<br />
<br />
[[Category:Work In Progress]]<br />
[[Category:Projects]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=User:Dan&diff=9557User:Dan2017-09-15T10:50:16Z<p>Dan.fhg: </p>
<hr />
<div>Dan is interested in growing Hakcuarium and sustainable Industrial Biotechnology.<br />
<br />
He can be reached at +41.79.836.4123 or dan.fhg@gmail.com<br />
<br />
Thanks for Reading!</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=User:Dan&diff=9556User:Dan2017-09-15T10:49:49Z<p>Dan.fhg: </p>
<hr />
<div>Dan is interested in growing Hakcuarium, sustainable DIY indutrial Biotechnology.<br />
<br />
He can be reached at +41.79.836.4123 or dan.fhg@gmail.com<br />
<br />
Thanks for Reading!</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=User:Dan&diff=9555User:Dan2017-09-15T10:49:05Z<p>Dan.fhg: Created page with "Dan is interested in growing Hakcuarium, sustainable DIY indutrial Biotechnology."</p>
<hr />
<div>Dan is interested in growing Hakcuarium, sustainable DIY indutrial Biotechnology.</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170911_Board_Meeting&diff=949120170911 Board Meeting2017-09-11T09:42:38Z<p>Dan.fhg: /* HTGA */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, Sept 11, 17-19pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chairs==<br />
* Vanessa<br />
* Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Anne-Laure<br />
*Daniel<br />
*Vanessa<br />
*Yann Pierson<br />
*Gianpaolo<br />
* Sam (by Skype via Gianpaolo)<br />
<br />
==Excused==<br />
<br />
*Luc<br />
*Yann Heurtaux<br />
<br />
==Not Heard from (to date, Sept 7)==<br />
<br />
*Ana (last note with excuses to RA on June 9th).<br />
<br />
<br />
=Agenda Items=<br />
<br />
==@Hello Board==<br />
(Vanessa, 1 min)<br />
<br />
==Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections? <br />
<br><br />
==Financial Status of the Association ==<br />
<br />
No news from Ana?<br><br />
A proposal has been made that Luc should become the treasurer again, since Ana has not been in contact recently.<br><br />
Who makes a motion that Luc will take up the position of Treasurer in addition to his role as Secretary?<br><br />
''to vote''<br />
<br />
==3rd Anniversary of the Association and Crowdfunding==<br />
(Vanessa, 10 minutes)<br />
The Hackuarium Association is 3 years old, as of September 2017 (exact dates?)<br><br />
Plans for big launch of crowdfunding on Weds, 18 October and Big Party with activities for Saturday, 28 October.<br><br />
Any objections? <br><br />
Join in for next crowdfunding meeting on 13Sept to help more, esp need video scenarios etc.<br> <br />
'''Edit YP:'''<br><br />
- Great! Won't be there on the 28th though.<br />
<br />
==Roles of Committee Members==<br />
(Everyone, 30 minutes)<br />
What do we each do? and what do we still need?? <br><br />
Community building <br><br />
Event organisation - especially working group for #OH nights <br><br />
UC coordination <br><br />
'''Edit YP'''<br><br />
- Clarification of the contribution I'm willing to make: My role is not to organize events. My role is to pass on the info on FB, MU and the wiki that other users want to promote. In that way the written content for the events is written consistently.<br><br />
- I'm willing to help on the lab organization when I have time and if needed.<br><br />
<br />
==Proposal for Internal Activities==<br />
How does the idea of monthly meetings with other UC/co-working/startup groups sound to you?<br><br />
Other ideas? <br><br />
<br />
[Sam] we'd like to redo our raspberry pi and electronics workshops in spring 2018.<br />
<br />
==Updates on External Activities==<br />
(Daniel, 30 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 21 to Sunday September 25, 21 to 25 for out-of-towners<br />
<br />
Hackuarium Representation (I would like to take the posters). Vanessa will be calling in. Any other actions?<br><br />
Community Sustainability<br />
<br />
===HTGA===<br />
<br />
Dan will get a call at 15h. Will update agenda after then<br />
<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
* very expensive<br />
* Potential Dutch student coming<br />
<br />
===Conference Series at Octanis@EPFL===<br />
<br />
[Sam]: Jonathan, Luc, Rachel come to Octanis @ EPFL and present Beerdecoded (3rd October), LivingInstruments, "Talk".<br />
- CONFIRM: We are going to file the first talk with Jonathan to EPFL this week. <br />
- NEXT ACTION: We will need to fix the other dates tonight if possible.<br />
<br />
==Other Business==<br />
<br />
<br />
=== Part time proposition ===<br />
Has this been pursued, since brought up by YannP for the Board meeting on 12July (and never really fully discussed)? <br><br />
de la dernière réunion de comité: by Yann P, <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
* Current status?<br />
<br />
Another potential part time salary from InArTiS might be to have a UC community/event manager again, as Yann H did in the old days. <br><br />
did Luc ever get compensation for event planning for UC? <br> it is clear Juliette needs extra help in organising UC with co-working and pro space in crises...<br />
<br><br />
'''Edit YP'''<br><br />
The UC situation is IMO too cahotic. UC is not the UC I immagined in the beginning. All working personnel in 3 years of UC left or got kicked reflecting potentially the poor organisation of the initiative. On a personnal level other projects took place. I'm therefore not pursuing the idea.<br />
<br />
=== Lab work ===<br />
(Gustavo, 20min) <br><br />
Flooring, water, ventilation... <br><br />
Interphone <br><br />
* Who plans to come in more regularly?<br><br />
* Will anyone use the co-working desks?? <br><br />
'''Edit YP'''<br><br />
I did a check on what is needed for launching the P1:<br><br />
- A list of SOP: https://docs.google.com/document/d/1F2yR6q7cbpKqdFqT9oQYrJjmkNryhMZGLI_b30JVJCA/edit <br><br />
- - To be modified to the current space<br><br />
- De-clutter the area to make it more microbio friendly<br><br />
- - Move what is unrelated to the activities in the other part specially in the cupboards<br><br />
- Add a clearly labelled biowaste container<br><br />
- Add the eye washers and mark them visibly <br><br />
- Add labels such as "wash your hands", "wear goggles"...<br><br />
- Fix the drawers so they don't fall.<br><br />
- Try Gustavos -60°C pelletier technique<br><br />
- water and air suction. <br><br />
Technically for the activities we'll adapt to the necessities.<br><br />
- Tell me before mid november what might be needed and we'll get it (buffers, enzymes, plastics, dH2O and ddH2O tanks ...)<br><br />
<br><br />
<br />
===More Activities===<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
RA going (will miss projected crowdfunding campaign launch day on the 18th, but back for the party...) <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... Maybe someone (Sam? [ Sam: won't be able to join in the end, exam prep time :( ] others?) also would like to come?<br />
Funds are being requested for RA and one more...<br><br />
<br />
*other? (Vanessa, please share your plans, too, in advance!)<br />
<br />
<br />
<br />
<br />
* <br />
* <br />
<br />
[[Category:Work In Progress]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170911_Board_Meeting&diff=949020170911 Board Meeting2017-09-11T05:50:44Z<p>Dan.fhg: /* HTGA */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, Sept 11, 17-19pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chairs==<br />
* Vanessa<br />
* Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Anne-Laure<br />
*Daniel<br />
*Vanessa<br />
*Yann Pierson<br />
*Gianpaolo<br />
* Sam (by Skype via Gianpaolo)<br />
<br />
==Excused==<br />
<br />
*Luc<br />
*Yann Heurtaux<br />
<br />
==Not Heard from (to date, Sept 7)==<br />
<br />
*Ana (last note with excuses to RA on June 9th).<br />
<br />
<br />
=Agenda Items=<br />
<br />
==@Hello Board==<br />
(Vanessa, 1 min)<br />
<br />
==Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections? <br />
<br><br />
==Financial Status of the Association ==<br />
<br />
No news from Ana?<br><br />
A proposal has been made that Luc should become the treasurer again, since Ana has not been in contact recently.<br><br />
Who makes a motion that Luc will take up the position of Treasurer in addition to his role as Secretary?<br><br />
''to vote''<br />
<br />
==3rd Anniversary of the Association and Crowdfunding==<br />
(Vanessa, 10 minutes)<br />
The Hackuarium Association is 3 years old, as of September 2017 (exact dates?)<br><br />
Plans for big launch of crowdfunding on Weds, 18 October and Big Party with activities for Saturday, 28 October.<br><br />
Any objections? <br><br />
Join in for next crowdfunding meeting on 13Sept to help more, esp need video scenarios etc.<br> <br />
'''Edit YP:'''<br><br />
- Great! Won't be there on the 28th though.<br />
<br />
==Roles of Committee Members==<br />
(Everyone, 30 minutes)<br />
What do we each do? and what do we still need?? <br><br />
Community building <br><br />
Event organisation - especially working group for #OH nights <br><br />
UC coordination <br><br />
'''Edit YP'''<br><br />
- Clarification of the contribution I'm willing to make: My role is not to organize events. My role is to pass on the info on FB, MU and the wiki that other users want to promote. In that way the written content for the events is written consistently.<br><br />
- I'm willing to help on the lab organization when I have time and if needed.<br><br />
<br />
==Proposal for Internal Activities==<br />
How does the idea of monthly meetings with other UC/co-working/startup groups sound to you?<br><br />
Other ideas? <br><br />
<br />
[Sam] we'd like to redo our raspberry pi and electronics workshops in spring 2018.<br />
<br />
==Updates on External Activities==<br />
(Daniel, 30 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 21 to Sunday September 25, 21 to 25 for out-of-towners<br />
<br />
Hackuarium Representation (I would like to take the posters). Vanessa will be calling in. Any other actions?<br><br />
Community Sustainability<br />
<br />
===HTGA===<br />
<br />
Dan will get a call at 18h. have questions ready before then!<br />
<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
* very expensive<br />
* Potential Dutch student coming<br />
<br />
===Conference Series at Octanis@EPFL===<br />
<br />
[Sam]: Jonathan, Luc, Rachel come to Octanis @ EPFL and present Beerdecoded (3rd October), LivingInstruments, "Talk".<br />
- CONFIRM: We are going to file the first talk with Jonathan to EPFL this week. <br />
- NEXT ACTION: We will need to fix the other dates tonight if possible.<br />
<br />
==Other Business==<br />
<br />
<br />
=== Part time proposition ===<br />
Has this been pursued, since brought up by YannP for the Board meeting on 12July (and never really fully discussed)? <br><br />
de la dernière réunion de comité: by Yann P, <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
* Current status?<br />
<br />
Another potential part time salary from InArTiS might be to have a UC community/event manager again, as Yann H did in the old days. <br><br />
did Luc ever get compensation for event planning for UC? <br> it is clear Juliette needs extra help in organising UC with co-working and pro space in crises...<br />
<br><br />
'''Edit YP'''<br><br />
The UC situation is IMO too cahotic. UC is not the UC I immagined in the beginning. All working personnel in 3 years of UC left or got kicked reflecting potentially the poor organisation of the initiative. On a personnal level other projects took place. I'm therefore not pursuing the idea.<br />
<br />
=== Lab work ===<br />
(Gustavo, 20min) <br><br />
Flooring, water, ventilation... <br><br />
Interphone <br><br />
* Who plans to come in more regularly?<br><br />
* Will anyone use the co-working desks?? <br><br />
'''Edit YP'''<br><br />
I did a check on what is needed for launching the P1:<br><br />
- A list of SOP: https://docs.google.com/document/d/1F2yR6q7cbpKqdFqT9oQYrJjmkNryhMZGLI_b30JVJCA/edit <br><br />
- - To be modified to the current space<br><br />
- De-clutter the area to make it more microbio friendly<br><br />
- - Move what is unrelated to the activities in the other part specially in the cupboards<br><br />
- Add a clearly labelled biowaste container<br><br />
- Add the eye washers and mark them visibly <br><br />
- Add labels such as "wash your hands", "wear goggles"...<br><br />
- Fix the drawers so they don't fall.<br><br />
- Try Gustavos -60°C pelletier technique<br><br />
- water and air suction. <br><br />
Technically for the activities we'll adapt to the necessities.<br><br />
- Tell me before mid november what might be needed and we'll get it (buffers, enzymes, plastics, dH2O and ddH2O tanks ...)<br><br />
<br><br />
<br />
===More Activities===<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
RA going (will miss projected crowdfunding campaign launch day on the 18th, but back for the party...) <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... Maybe someone (Sam? [ Sam: won't be able to join in the end, exam prep time :( ] others?) also would like to come?<br />
Funds are being requested for RA and one more...<br><br />
<br />
*other? (Vanessa, please share your plans, too, in advance!)<br />
<br />
<br />
<br />
<br />
* <br />
* <br />
<br />
[[Category:Work In Progress]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170911_Board_Meeting&diff=948920170911 Board Meeting2017-09-11T05:39:03Z<p>Dan.fhg: /* Other Business */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, Sept 11, 17-19pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chairs==<br />
* Vanessa<br />
* Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Anne-Laure<br />
*Daniel<br />
*Vanessa<br />
*Yann Pierson<br />
*Gianpaolo<br />
* Sam (by Skype via Gianpaolo)<br />
<br />
==Excused==<br />
<br />
*Luc<br />
*Yann Heurtaux<br />
<br />
==Not Heard from (to date, Sept 7)==<br />
<br />
*Ana (last note with excuses to RA on June 9th).<br />
<br />
<br />
=Agenda Items=<br />
<br />
==@Hello Board==<br />
(Vanessa, 1 min)<br />
<br />
==Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections? <br />
<br><br />
==Financial Status of the Association ==<br />
<br />
No news from Ana?<br><br />
A proposal has been made that Luc should become the treasurer again, since Ana has not been in contact recently.<br><br />
Who makes a motion that Luc will take up the position of Treasurer in addition to his role as Secretary?<br><br />
''to vote''<br />
<br />
==3rd Anniversary of the Association and Crowdfunding==<br />
(Vanessa, 10 minutes)<br />
The Hackuarium Association is 3 years old, as of September 2017 (exact dates?)<br><br />
Plans for big launch of crowdfunding on Weds, 18 October and Big Party with activities for Saturday, 28 October.<br><br />
Any objections? <br><br />
Join in for next crowdfunding meeting on 13Sept to help more, esp need video scenarios etc.<br> <br />
'''Edit YP:'''<br><br />
- Great! Won't be there on the 28th though.<br />
<br />
==Roles of Committee Members==<br />
(Everyone, 30 minutes)<br />
What do we each do? and what do we still need?? <br><br />
Community building <br><br />
Event organisation - especially working group for #OH nights <br><br />
UC coordination <br><br />
'''Edit YP'''<br><br />
- Clarification of the contribution I'm willing to make: My role is not to organize events. My role is to pass on the info on FB, MU and the wiki that other users want to promote. In that way the written content for the events is written consistently.<br><br />
- I'm willing to help on the lab organization when I have time and if needed.<br><br />
<br />
==Proposal for Internal Activities==<br />
How does the idea of monthly meetings with other UC/co-working/startup groups sound to you?<br><br />
Other ideas? <br><br />
<br />
[Sam] we'd like to redo our raspberry pi and electronics workshops in spring 2018.<br />
<br />
==Updates on External Activities==<br />
(Daniel, 30 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 21 to Sunday September 25, 21 to 25 for out-of-towners<br />
<br />
Hackuarium Representation (I would like to take the posters). Vanessa will be calling in. Any other actions?<br><br />
Community Sustainability<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
* very expensive<br />
* Potential Dutch student coming<br />
===Conference Series at Octanis@EPFL===<br />
<br />
[Sam]: Jonathan, Luc, Rachel come to Octanis @ EPFL and present Beerdecoded (3rd October), LivingInstruments, "Talk".<br />
- CONFIRM: We are going to file the first talk with Jonathan to EPFL this week. <br />
- NEXT ACTION: We will need to fix the other dates tonight if possible.<br />
<br />
==Other Business==<br />
<br />
<br />
=== Part time proposition ===<br />
Has this been pursued, since brought up by YannP for the Board meeting on 12July (and never really fully discussed)? <br><br />
de la dernière réunion de comité: by Yann P, <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
* Current status?<br />
<br />
Another potential part time salary from InArTiS might be to have a UC community/event manager again, as Yann H did in the old days. <br><br />
did Luc ever get compensation for event planning for UC? <br> it is clear Juliette needs extra help in organising UC with co-working and pro space in crises...<br />
<br><br />
'''Edit YP'''<br><br />
The UC situation is IMO too cahotic. UC is not the UC I immagined in the beginning. All working personnel in 3 years of UC left or got kicked reflecting potentially the poor organisation of the initiative. On a personnal level other projects took place. I'm therefore not pursuing the idea.<br />
<br />
=== Lab work ===<br />
(Gustavo, 20min) <br><br />
Flooring, water, ventilation... <br><br />
Interphone <br><br />
* Who plans to come in more regularly?<br><br />
* Will anyone use the co-working desks?? <br><br />
'''Edit YP'''<br><br />
I did a check on what is needed for launching the P1:<br><br />
- A list of SOP: https://docs.google.com/document/d/1F2yR6q7cbpKqdFqT9oQYrJjmkNryhMZGLI_b30JVJCA/edit <br><br />
- - To be modified to the current space<br><br />
- De-clutter the area to make it more microbio friendly<br><br />
- - Move what is unrelated to the activities in the other part specially in the cupboards<br><br />
- Add a clearly labelled biowaste container<br><br />
- Add the eye washers and mark them visibly <br><br />
- Add labels such as "wash your hands", "wear goggles"...<br><br />
- Fix the drawers so they don't fall.<br><br />
- Try Gustavos -60°C pelletier technique<br><br />
- water and air suction. <br><br />
Technically for the activities we'll adapt to the necessities.<br><br />
- Tell me before mid november what might be needed and we'll get it (buffers, enzymes, plastics, dH2O and ddH2O tanks ...)<br><br />
<br><br />
<br />
===More Activities===<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
RA going (will miss projected crowdfunding campaign launch day on the 18th, but back for the party...) <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... Maybe someone (Sam? [ Sam: won't be able to join in the end, exam prep time :( ] others?) also would like to come?<br />
Funds are being requested for RA and one more...<br><br />
<br />
*other? (Vanessa, please share your plans, too, in advance!)<br />
<br />
<br />
<br />
<br />
* <br />
* <br />
<br />
[[Category:Work In Progress]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170911_Board_Meeting&diff=948820170911 Board Meeting2017-09-11T05:37:03Z<p>Dan.fhg: /* Updates on External Activities */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, Sept 11, 17-19pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chairs==<br />
* Vanessa<br />
* Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Anne-Laure<br />
*Daniel<br />
*Vanessa<br />
*Yann Pierson<br />
*Gianpaolo<br />
* Sam (by Skype via Gianpaolo)<br />
<br />
==Excused==<br />
<br />
*Luc<br />
*Yann Heurtaux<br />
<br />
==Not Heard from (to date, Sept 7)==<br />
<br />
*Ana (last note with excuses to RA on June 9th).<br />
<br />
<br />
=Agenda Items=<br />
<br />
==@Hello Board==<br />
(Vanessa, 1 min)<br />
<br />
==Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections? <br />
<br><br />
==Financial Status of the Association ==<br />
<br />
No news from Ana?<br><br />
A proposal has been made that Luc should become the treasurer again, since Ana has not been in contact recently.<br><br />
Who makes a motion that Luc will take up the position of Treasurer in addition to his role as Secretary?<br><br />
''to vote''<br />
<br />
==3rd Anniversary of the Association and Crowdfunding==<br />
(Vanessa, 10 minutes)<br />
The Hackuarium Association is 3 years old, as of September 2017 (exact dates?)<br><br />
Plans for big launch of crowdfunding on Weds, 18 October and Big Party with activities for Saturday, 28 October.<br><br />
Any objections? <br><br />
Join in for next crowdfunding meeting on 13Sept to help more, esp need video scenarios etc.<br> <br />
'''Edit YP:'''<br><br />
- Great! Won't be there on the 28th though.<br />
<br />
==Roles of Committee Members==<br />
(Everyone, 30 minutes)<br />
What do we each do? and what do we still need?? <br><br />
Community building <br><br />
Event organisation - especially working group for #OH nights <br><br />
UC coordination <br><br />
'''Edit YP'''<br><br />
- Clarification of the contribution I'm willing to make: My role is not to organize events. My role is to pass on the info on FB, MU and the wiki that other users want to promote. In that way the written content for the events is written consistently.<br><br />
- I'm willing to help on the lab organization when I have time and if needed.<br><br />
<br />
==Proposal for Internal Activities==<br />
How does the idea of monthly meetings with other UC/co-working/startup groups sound to you?<br><br />
Other ideas? <br><br />
<br />
[Sam] we'd like to redo our raspberry pi and electronics workshops in spring 2018.<br />
<br />
==Updates on External Activities==<br />
(Daniel, 30 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 21 to Sunday September 25, 21 to 25 for out-of-towners<br />
<br />
Hackuarium Representation (I would like to take the posters). Vanessa will be calling in. Any other actions?<br><br />
Community Sustainability<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
* very expensive<br />
* Potential Dutch student coming<br />
===Conference Series at Octanis@EPFL===<br />
<br />
[Sam]: Jonathan, Luc, Rachel come to Octanis @ EPFL and present Beerdecoded (3rd October), LivingInstruments, "Talk".<br />
- CONFIRM: We are going to file the first talk with Jonathan to EPFL this week. <br />
- NEXT ACTION: We will need to fix the other dates tonight if possible.<br />
<br />
==Other Business==<br />
<br />
<br />
=== Part time proposition ===<br />
Has this been pursued, since brought up by YannP for the Board meeting on 12July (and never really fully discussed)? <br><br />
de la dernière réunion de comité: by Yann P, <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
* Current status?<br />
<br />
Another potential part time salary from InArTiS might be to have a UC community/event manager again, as Yann H did in the old days. <br><br />
did Luc ever get compensation for event planning for UC? <br> it is clear Juliette needs extra help in organising UC with co-working and pro space in crises...<br />
<br><br />
'''Edit YP'''<br><br />
The UC situation is IMO too cahotic. UC is not the UC I immagined in the beginning. All working personnel in 3 years of UC left or got kicked reflecting potentially the poor organisation of the initiative. On a personnal level other projects took place. I'm therefore not pursuing the idea.<br />
<br />
=== Lab work ===<br />
(Gustavo, 20min) <br><br />
Flooring, water, ventilation... <br><br />
Interphone <br><br />
* Who plans to come in more regularly?<br><br />
* Will anyone use the co-working desks?? <br><br />
'''Edit YP'''<br><br />
I did a check on what is needed for launching the P1:<br><br />
- A list of SOP: https://docs.google.com/document/d/1F2yR6q7cbpKqdFqT9oQYrJjmkNryhMZGLI_b30JVJCA/edit <br><br />
- - To be modified to the current space<br><br />
- De-clutter the area to make it more microbio friendly<br><br />
- - Move what is unrelated to the activities in the other part specially in the cupboards<br><br />
- Add a clearly labelled biowaste container<br><br />
- Add the eye washers and mark them visibly <br><br />
- Add labels such as "wash your hands", "wear goggles"...<br><br />
- Fix the drawers so they don't fall.<br><br />
- Try Gustavos -60°C pelletier technique<br><br />
- And off course the water and air succion. Flooring is overkill who gives a damn... <br><br />
Technically for the activities we'll adapt to the necessities.<br><br />
- Tell me before mid november what might be needed and we'll get it (buffers, enzymes, plastics, dH2O and ddH2O tanks ...)<br><br />
<br><br />
<br />
===More Activities===<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
RA going (will miss projected crowdfunding campaign launch day on the 18th, but back for the party...) <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... Maybe someone (Sam? [ Sam: won't be able to join in the end, exam prep time :( ] others?) also would like to come?<br />
Funds are being requested for RA and one more...<br><br />
<br />
*other? (Vanessa, please share your plans, too, in advance!)<br />
<br />
<br />
<br />
<br />
* <br />
* <br />
<br />
[[Category:Work In Progress]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170726_Seance_Crowdfunding1&diff=940320170726 Seance Crowdfunding12017-07-26T14:30:41Z<p>Dan.fhg: /* Point 2: Crowdfunding campaign practical details */</p>
<hr />
<div>Working on the Crowdfunding Strategy for Hackuarium!! <br><br />
Looking forward for a successful campaign!<br><br />
<br />
'''Agenda of Crowdfunding 1 Meeting'''<br />
<br />
Date: Wednesday, July 26th, 18.30-19.30pm<br />
<br><br />
Place: 2nd floor meeting room 'bibliotech'<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Joël<br />
* Olivier<br />
* Yann Pierson<br />
* Roger<br />
*<br />
<br />
==Excused==<br />
*Luc<br />
*Anne-Laure<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
=Crowdfunding Planning=<br />
<br />
==@Hello Crowdfunding Working Group==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all crowdfunding working group members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections? <br />
<br />
<br />
==Point 1: Spin for Campaign?==<br />
<br />
Taking Hackuarium to the next level? <br />
Helping projects move forward?<br />
or?<br />
<br />
c'était un peu le fin de la discussion du jeudi 6 juillet. <br> Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
==Point 2: Crowdfunding campaign practical details==<br />
(Rachel, 15 min)<br><br />
<br />
===WeMakeIt Science Booster Campaign===<br />
<br />
'''Timeline''' <br><br />
Now - 15 August: Collect, write and upload story (COM), perks (RWD), short pitch (RWD), Budget (WG) on wiki. <br><br />
15 August - 30 August: Build campaign (WG) on wemakeit.com website. <br><br />
15 September - 15 October: Run campaign (WG+COM). <br><br />
<br />
Launch planned for the '''15 September''' 2017 <br><br />
<br />
Content coordination: Rachel <br><br />
Communication: Joël <br><br />
Rewards: Olivier <br><br />
<br />
'''Contribution Ideas so far obtained from: <br><br />
<br />
Anne-Laure/ Rachel: T-shirts Hackuarium<br />
<br><br />
<br />
Gustavo: SpectroPointer Workshop<br />
<br><br />
<br />
Yann P: Molecular Biology Workshop<br />
<br><br />
<br />
Rachel: Foldscope/cheek cells/moss life Workshop <br />
<br><br />
<br />
Luc P: Spectrophotometer Workshop<br />
<br><br />
<br />
Dan: Metabolic Modeling Crash Course<br />
<br><br />
<br />
Luc H: A bio-music concert by Living Instruments<br />
<br><br />
<br />
etc.<br />
<br />
<br><br />
<br />
===Teaser Video, Launch Video, Campaign 'spice' Video(s) ===<br />
Who can help?<br><br />
Scenarios?<br><br />
project specific<br><br />
space specific<br><br />
central theme important!<br><br />
<br />
Filming may be possible even in 2nd floor studio: to talk to Leila! <br><br />
<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
'''Teaser of the crowdfunding!<br><br />
'''le 30 août!<br><br />
A party! <br><br />
Une fête!<br />
<br> <br />
Invitons nos membres, amies Hackuarium, voisines et voisins du bâtiment Ateliers de Renens et public à<br />
fêter nos 3 ans et construire ensemble<br />
<br />
<br />
<br><br />
<br />
===Fête Hackuarium to announce the Launch of Crowdfunding Campaign===<br />
le 13 sept...<br />
<br />
<br><br />
<br />
'''Events to be announced well in advance!</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935820170712 Board Meeting2017-07-12T16:30:39Z<p>Dan.fhg: /* Daniel Updates */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10-15 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 21 to Sunday September 25, 21 to 25 for out-of-towners<br />
<br />
funding: likely partial, full funding to be confirmed<br />
<br />
Summit structure:<br />
*All Labs: Presentation format: 4 min presentation, 1 min what we want from the summit<br />
*Projects will be presented too. <br />
*Workshops are being done. <br />
*Freedom, People can do their thing.<br />
*Final day we will talk about doing global collaborations<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
<br />
Things to think about:<br />
*If you had George church for a day, what would you do.<br />
*Joey ITO is also around.<br />
*Is Hackuarium on DIYbio mailing list<br />
*Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
$$$<br />
*degree costs 5k <br />
*2.5k goes to us, we do whatever we want<br />
<br />
Passing<br />
*Class-once you pay certificate requires passing grade in every topic<br />
*Final project uses at least 3 topics presented in January<br />
*You can take multiple years to pass.<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
===more points===<br />
<br />
*Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
*Should we draft an agreement of conditions for the lab space between Hackuarium and UC/pro-space? <br />
<br />
*What do you guys think about proposing a role for community growth? (Daniel)<br />
<br />
[[Category:Board Meetings]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935720170712 Board Meeting2017-07-12T16:28:32Z<p>Dan.fhg: /* Bio Summit */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 21 to Sunday September 25, 21 to 25 for out-of-towners<br />
<br />
funding: likely partial, full funding to be confirmed<br />
<br />
Summit structure:<br />
*All Labs: Presentation format: 4 min presentation, 1 min what we want from the summit<br />
*Projects will be presented too. <br />
*Workshops are being done. <br />
*Freedom, People can do their thing.<br />
*Final day we will talk about doing global collaborations<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
<br />
Things to think about:<br />
*If you had George church for a day, what would you do.<br />
*Joey ITO is also around.<br />
*Is Hackuarium on DIYbio mailing list<br />
*Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
$$$<br />
*degree costs 5k <br />
*2.5k goes to us, we do whatever we want<br />
<br />
Passing<br />
*Class-once you pay certificate requires passing grade in every topic<br />
*Final project uses at least 3 topics presented in January<br />
*You can take multiple years to pass.<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
===more points===<br />
<br />
*Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
*Should we draft an agreement of conditions for the lab space between Hackuarium and UC/pro-space? <br />
<br />
*What do you guys think about proposing a role for community growth? (Daniel)<br />
<br />
[[Category:Board Meetings]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935620170712 Board Meeting2017-07-12T16:27:25Z<p>Dan.fhg: /* Bio Summit */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 22 to Sunday September 24<br />
<br />
funding: likely partial, full funding to be confirmed<br />
<br />
Summit structure:<br />
*All Labs: Presentation format: 4 min presentation, 1 min what we want from the summit<br />
*Projects will be presented too. <br />
*Workshops are being done. <br />
*Freedom, People can do their thing.<br />
*Final day we will talk about doing global collaborations<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
<br />
Things to think about:<br />
*If you had George church for a day, what would you do.<br />
*Joey ITO is also around.<br />
*Is Hackuarium on DIYbio mailing list<br />
*Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
$$$<br />
*degree costs 5k <br />
*2.5k goes to us, we do whatever we want<br />
<br />
Passing<br />
*Class-once you pay certificate requires passing grade in every topic<br />
*Final project uses at least 3 topics presented in January<br />
*You can take multiple years to pass.<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
===more points===<br />
<br />
*Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
*Should we draft an agreement of conditions for the lab space between Hackuarium and UC/pro-space? <br />
<br />
*What do you guys think about proposing a role for community growth? (Daniel)<br />
<br />
[[Category:Board Meetings]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935520170712 Board Meeting2017-07-12T16:25:26Z<p>Dan.fhg: /* Part time proposition */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 22 to Sunday September 24<br />
<br />
funding: potentially available<br />
<br />
Summit structure:<br />
*All Labs: Presentation format: 4 min presentation, 1 min what we want from the summit<br />
*Projects will be presented too. <br />
*Workshops are being done. <br />
*Freedom, People can do their thing.<br />
*Final day we will talk about doing global collaborations<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
<br />
Things to think about:<br />
*If you had George church for a day, what would you do.<br />
*Joey ITO is also around.<br />
*Is Hackuarium on DIYbio mailing list<br />
*Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
$$$<br />
*degree costs 5k <br />
*2.5k goes to us, we do whatever we want<br />
<br />
Passing<br />
*Class-once you pay certificate requires passing grade in every topic<br />
*Final project uses at least 3 topics presented in January<br />
*You can take multiple years to pass.<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
===more points===<br />
<br />
*Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
*Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
*Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
*Should we draft an agreement of conditions for the lab space between Hackuarium and UC/pro-space? <br />
<br />
*What do you guys think about proposing a role for community growth? (Daniel)<br />
<br />
[[Category:Board Meetings]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935420170712 Board Meeting2017-07-12T16:24:46Z<p>Dan.fhg: /* Point 7: Other Business */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 22 to Sunday September 24<br />
<br />
funding: potentially available<br />
<br />
Summit structure:<br />
*All Labs: Presentation format: 4 min presentation, 1 min what we want from the summit<br />
*Projects will be presented too. <br />
*Workshops are being done. <br />
*Freedom, People can do their thing.<br />
*Final day we will talk about doing global collaborations<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
<br />
Things to think about:<br />
*If you had George church for a day, what would you do.<br />
*Joey ITO is also around.<br />
*Is Hackuarium on DIYbio mailing list<br />
*Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
$$$<br />
*degree costs 5k <br />
*2.5k goes to us, we do whatever we want<br />
<br />
Passing<br />
*Class-once you pay certificate requires passing grade in every topic<br />
*Final project uses at least 3 topics presented in January<br />
*You can take multiple years to pass.<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
Should we draft an agreement of conditions for the lab space between Hackuarium and UC/pro-space? <br />
<br />
What do you guys think about proposing a role for community growth? (Daniel)<br />
<br />
[[Category:Board Meetings]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935320170712 Board Meeting2017-07-12T16:22:47Z<p>Dan.fhg: /* Daniel Updates */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 22 to Sunday September 24<br />
<br />
funding: potentially available<br />
<br />
Summit structure:<br />
*All Labs: Presentation format: 4 min presentation, 1 min what we want from the summit<br />
*Projects will be presented too. <br />
*Workshops are being done. <br />
*Freedom, People can do their thing.<br />
*Final day we will talk about doing global collaborations<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
<br />
Things to think about:<br />
*If you had George church for a day, what would you do.<br />
*Joey ITO is also around.<br />
*Is Hackuarium on DIYbio mailing list<br />
*Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
$$$<br />
*degree costs 5k <br />
*2.5k goes to us, we do whatever we want<br />
<br />
Passing<br />
*Class-once you pay certificate requires passing grade in every topic<br />
*Final project uses at least 3 topics presented in January<br />
*You can take multiple years to pass.<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
[[Category:Board Meetings]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935220170712 Board Meeting2017-07-12T15:19:01Z<p>Dan.fhg: /* Daniel Updates */</p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
<br />
dates: Friday September 22 to Sunday September 24<br />
<br />
funding: potentially available<br />
<br />
Summit structure:<br />
*All Labs: Presentation format: 4 min presentation, 1 min what we want from the summit<br />
*Projects will be presented too. <br />
*Workshops are being done. <br />
*Freedom, People can do their thing.<br />
*Final day we will talk about doing global collaborations<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
<br />
Things to think about:<br />
*If you had George church for a day, what would you do.<br />
*Joey ITO is also around.<br />
*Is Hackuarium on DIYbio mailing list<br />
*Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br><br />
Bio.academany.org<br><br />
Design homework and experimental <br><br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
$$$<br />
*degree costs 5k <br />
*2.5k goes to us, we do whatever we want<br />
<br />
Passing<br />
*Class-once you pay certificate requires passing grade in every topic<br />
*Final project uses at least 3 topics presented in January<br />
*You can take multiple years to pass.<br />
<br />
===Salary===<br />
What do you guys think about proposing a role for community growth?<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
[[Category:Board Meetings]]</div>Dan.fhghttps://wiki.hackuarium.ch/index.php?title=20170712_Board_Meeting&diff=935120170712 Board Meeting2017-07-12T15:00:45Z<p>Dan.fhg: </p>
<hr />
<div>'''Agenda of the Board Meeting'''<br />
<br />
Date: Wednesday, July 12th, 18.30-19.30pm<br />
<br><br />
Place: UniverCité, 2rd floor meeting room<br />
<br />
__TOC__<br />
<br><br />
=Composition=<br />
<br />
==Chair==<br />
*Rachel<br />
<br />
==Present==<br />
* Gustavo<br />
*Yann Pierson<br />
*Anne-Laure<br />
*Luc<br />
<br />
==Excused==<br />
<br />
*Gianpaolo<br />
*Vanessa<br />
*Yann Heurtaux<br />
*Daniel<br />
<br />
<br />
==Not Heard From (to date, 11.7)==<br />
<br />
*Sam<br />
*Ana<br />
<br />
=Items=<br />
<br />
==@Hello Board==<br />
(Rachel, 1 min)<br />
<br />
==Point 0: Public minutes==<br />
(Rachel, 1 min)<br />
<br />
Get ok from all board members to upload minutes on wiki (or else discuss alternative below)<br />
<br><br />
<br />
Objections?<br />
<br />
==Point 1: Financial Report==<br />
<br />
No news from Ana?<br />
<br />
suivi de membres ?<br />
mécanismes de paiement UC Cartes d'accès ?<br />
<br />
==Point 2: Moving into the New space==<br />
(Rachel, 10 minutes)<br />
<br />
===Déménagement===<br />
<br />
Comment cela s'est-il passé? Est-ce que quelqu'un a des remarques? <br><br />
Comment organiser l'utilisation de tous ?<br><br />
Derniers trucs de 1ière!!<br />
<br />
===Inauguration 27 June===<br />
<br />
Comment cela s'est-il passé?<br />
<br />
===Fête Hackuarium 3éme anniversaire & New Space===<br />
We need a party!<br />
Nous avons envie d'une fête social ouverte. <br />
Invitons nos membres, amies hackuarium, on essaye de atirer nos voisines et voisin du bâtiment Ateliers de Renens et public, <br />
fêter nos 3 ans de contruire un projet ensemble?<br />
Beginning of the crowdfunding?<br />
Tout?<br />
<br><br />
<br />
==Point 3: Crowdfunding campaign==<br />
(Rachel, 15 min)<br><br />
<br />
Très court résumé de la discussion du jeudi 6 juillet. Pour plus d'information, voir [[20170706_BrnstrmCF | ici]]<br />
<br />
Tout le monde est ok avec la narration? L'équipe de pilotage de la campagne va faire une proposition de budget et d'utilisation des fonds levés lors du prochain board meeting.<br />
<br />
===UC Interactions===<br />
<br />
Meeting between Benoit and Luc never happened. <br><br />
Vanessa did talk to him, and some charm was turned on ('whatever you want, let me know'), but concrete details are still lacking...<br><br />
In regards to crowdfunding, apparently, we only need to tell UC that we will run the campaign and that we hope for their continued support.<br><br />
<br />
--> Vanessa, proposes ??<br><br />
<br />
Luc points out: Benoit does not work by email. Call him, meet him, but no need to ask questions by email (to Christophe either).<br><br />
<br />
Labo: water, ventilation, ice machine, -80 freezer?? Yann Pierson (5min) <br><br />
<br />
Visitors: when people come (especially if they just pass through without introducing themselves) we should [https://docs.google.com/spreadsheets/d/15CnsBzEbvwi6gq3s6ytn24ca0xEi-WrsMuKDMqdPqFc/edit?usp=sharing continue to fill in the google drive document] to help document the added value to UC from our efforts.<br />
<br />
==Point 4: Minigrants==<br />
<br />
We need to clean the process to ask for and validate minigrants. It would be good to agree on a strict process and put it on the wiki.<br><br />
<br />
Initially, the request would be forwarded to the whole board, and 3 board members voting in favour would unlock a mini grant.<br><br />
<br />
Maybe 1 week notice to object is more appropriate now that we are more people in the board?<br><br />
<br />
==Point 5: Next board meetings==<br />
<br />
10 août?<br><br />
13 septembre? <br><br />
etc.<br><br />
<br />
==Point 6: La Nébuleuse==<br />
<br />
Accès du personnel de la Nébuleuse à Hackuarium et au laboratoire en général. <br><br />
<br />
Ils n'ont jamais vraiment payé quoi que ce soit.. Donc faut-il discuter et revoir notre politique vis-à-vis d'eux, ou alors les envoyer vers Benoit et qu'il s'arrange avec lui?? En fait, ils vont déménager en bas (sur l'autre côte du maker space), donc, on sait pas combien de temps une arrangement va être nécessaire.<br />
<br><br />
Yann P: De ce que je sais ils gardent les deux zones, donc ils prennent la zone vers le makerspace pour faire leur bières "mainstream" et le reste pour le "R&D". <br><br />
De plus ils ont reçu de larges financements (to be confirmed by a second source) donc l'argent ne manque pas. <br><br />
Il y a eu une proposition à l'époque ou YH bossait avec eux de financer une nouvelle autoclave. Je suis d'avis de changer leur politique d'utilisation. Soit ils contribuent de manière financière plus sérieusement soit ils nous aident à investir dans des instruments.<br />
<br />
==Daniel Updates==<br />
(Daniel, 10 minutes)<br />
=== Bio Summit ===<br />
web site: https://www.biosummit.org/<br />
dates: Friday September 22 to Sunday September 24<br />
funding: potentially available<br />
<br />
Presentation format: 4 min presentation, 1 min what we want from the summit<br />
<br />
Potential Topics for Hackuarium<br />
* Open innovation topic. How are we doing things differently than before<br />
* Sustainability economics<br />
* Daniel: I would like to talk about economic sustainability and give a workshop<br />
Projects will be presented too. Workshops are being done too. People can do their thing.<br />
<br />
Final day we will talk about doing global collaborations<br />
<br />
Things to think about:<br />
If you had George church for a day, what would you do.<br />
Joey ITO is also around.<br />
Is Hackuarium on DIYbio mailing list<br />
Hackuarium should re-post the conference social media<br />
<br />
===HTGA===<br />
Starts sept 13th 14 sessions -> every week <br />
Bio.academany.org<br />
Design homework and experimental <br />
<br />
They would be very happy to have us join: "We are the world innovator”<br />
We could be a hub<br />
<br />
We get packages fab academy <br />
* how to make anything<br />
* How to grow anything<br />
<br />
degree costs 5k <br />
2.5k goes to us, we do whatever we want<br />
<br />
Commit<br />
Class-once you pay certificate requires passing grade in every topic<br />
Final project uses at least 3 topics presented in January<br />
You can take multiple years to pass.<br />
<br />
===Salary===<br />
What do you guys think about proposing a role for community growth?<br />
<br />
==Point 7: Other Business==<br />
<br />
=== Part time proposition ===<br />
<br />
Yann P: <br />
Je veux négocier avec UC la création d'un poste de lab manager à temps partiel.<br><br />
Il manque dans l'infrastructure du materiel et des nécessités techniques:<br><br />
:<li> Mise en place dans le P1 et la zone Pro d'evacuation d'air.<br />
:<li> Structuration du P1 pour un espace de microbiologie en commun i.e. :<br><br />
::<li> 2 à 3 freezer + fridge (1x stock de chimiques commun, 2x stock de projets)<br />
::<li> une machine à glace<br />
::<li> un -80° (petit)<br />
::<li> un incubateur shaker<br />
<br />
Pour la zone pro je propose le cahier des charges suivant:<br><br />
:<li> Mise en contact avec les fournisseurs et services i.e. :<br><br />
::<li> Mise en place du service de séquençage<br />
::<li> Mise en place du service de synthèse de genes<br />
::<li> Achat de chimiques (Sigma, CarlRoth, AppliChem, Abcam)<br />
::<li> Achat de consommables et plastiques (VWR, Huberlab, TPP)<br />
:<li> Contact avec les startups i.e. :<br><br />
::<li> Besoins techniques et compétences<br />
::<li> Mise en place de leur zone de travail<br />
::<li> Formation aux règles de sécurité de la zone<br />
:<li> Aide à la mise en place de la partie chimique et analytique (à définir selon BD):<br><br />
::<li> Analyse de base, HPLC voir LC-MS, lecture de plaque (Abs, Fluo voir Biolum)<br />
::<li> Chimie légère et de fait mise en place de l'élimination des déchets chimiques.<br />
:<li> Amener du réalisme dans l'idée et lâcher les incubateurs de 2000L.<br />
<br />
Mise en place aussi dans un second temps de formation au travers de workshops<br />
:<li> Basic biology handling (pipetting, working small scale, buffer preparation, basic chemistry)<br />
:<li> Cloning (Restriction ligation, Gibson cloning, heat-shock and electrocompetent bacteria, vectors...)<br />
:<li> Protein expression (small scale protein expression, fluorescent protein incorporation)<br />
:<li> Basic sensor creation (FRET fluorescent reporters, gene reporters)<br />
<br />
Is anybody against ? <br />
<br />
<br><br />
<br />
Mozilla Science Mini-Grant - delayed announcement, by end of July? <br><br />
<br />
Edgeryders Open Village Festival 2017, 19-21 October, Brussels <br><br />
<br />
Belgrade Science Festival - December 2017 - interested in Foldscope, but also music and art hacks... <br><br />
<br />
[[Category:Board Meetings]]</div>Dan.fhg