BioP transfection

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Transfection.png

Introduction

  • The transfection is the process of inserting a plasmid inside a bacteria. Several types of bacteria can be used as host for the plasmid and will usually express the information contained in this plasmid afterwards.
  • Electro competent way

  • Here the bacteria is inserted with the plasmid inside a cuvette. The cuvette will be added to an electroporator and an electric current will be applied tearing holes in the bacteria forcing the plasmid inside.
  • The bacteria is transferred to an aliquot and the medium is added for it to recover in a heat-shaker.
  • The voltage is usually 1700V but can vary depending on the bacteria you use.
  • Chemo competent way

  •  !!! HAS TO BE CORRECTED ON DRAWING AND TEXT HAS TO BE ADDED !!!

  • Protocols


    Safety


    Hackuarium Specifics