Test by YP
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| What is the purpose of the GMO / protein you want to create? | I need a bioluminescence for a art project (buffer test: aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa) |
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| Describe why you require lab access to achieve your goal? | I will encode the Lux operon in e.coli |
| Which plasmid will you use? | pET-51b(+) |
| Plasmid cassette code | Test of real cassette length (note we could restrict this field te the atgc chars): TGGAGCCACCCGCAGTTCGAAAAGGGTGCAGATGACGACGACAAGGTACCGCATATGGATACCGTTCCGTGGTTTCCGCGTACCATTCAAGAACTGGATCGTTTTGCAAATCAGATTCTGAGCTATGGTGCCGAACTGGATGCAGATCATCCGGGTTTTAAAGATCCGGTTTATCGTGCACGTCGTAAACAGTTTGCAGATATCGCATATAACTATCGTCATGGTCAGCCGATTCCGCGTGTTGAATATATGGAAGAAGAGAAAAAAACCTGGGGCACCGTGTTTAAAACACTGAAAAGCCTGTATAAAACCCACGCCTGCTATGAATATAACCATATTTTTCCGCTGCTGGAAAAATACTGCGGCTTTCATGAAGATAACATCCCGCAGCTGGAAGATGTTAGCCAGTTTCTGCAGACCTGTACCGGTTTTCGTCTGCGTCCGGTTGCAGGTCTGCTGAGCAGCCGTGATTTTCTGGGTGGTCTGGCATTTCGTGTTTTTCATTGTACCCAGTATATTCGCCATGGTAGCAAACCGATGTATACACCGGAACCGGATATTTGTCATGAACTGCTGGGTCATGTTCCGCTGTTTAGCGATCGTAGCTTTGCACAGTTTAGCCAAGAAATTGGTCTGGCCAGCCTGGGTGCACCGGATGAATATATTGAAAAACTGGCAACCATCTACTGGTTCACCGTTGAATTTGGTCTGTGTAAACAGGGCGATAGCATTAAAGCCTATGGTGCTGGTCTGCTGTCTAGCTTTGGTGAACTGCAGTATTGTCTGAGCGAAAAACCGAAACTGCTGCCGCTGGAACTGGAAAAAACCGCAATTCAGAATTATACCGTGACCGAATTTCAGCCGCTGTATTACGTTGCAGAAAGTTTTAATGATGCCAAAGAAAAAGTGCGCAATTTCGCAGCAACCATTCCGCGTCCGTTTAGCGTTCGTTATGATCCGTATACCCAGCGTATTGAAGTTCTGGATAATACCCAGCAACTGAAAATTCTGGCAGATAGCATCAATAGCGAAATTGGTATTCTGTGTAGCGCACTGCAGAAAATCAAAGGAGCTCCGGGCTTCTCCTCAATTTCCGCTCATCACCACCATCATCACCATCACCACCACT |
| Peptide translation of above cassette | WSHPQFEKGADDDDKVPHMDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIKGAPGFSSISAHHHHHHHHHH |
| If you want to use several building blocks for you proteins, give details on the templates | temp 1: ypp1: contains the pET-51b(+) vector, ypp2: contains the ... |
| Give the primers you are going to use to realise your clones | P1_F : TGGTAGTGAAGCAAGCGCCAGCATTATTG, tm = 66°C |
Nice start: What could be needed: - big box for the cassette sequence - big box for translation - several entries for tamplate as [name] [description] - several entries for primers as [name] [sequence] [tm (melting temperature)]
<pmap id="test" restriction-enzymes="ecori" gb-location="http://www.ncbi.nlm.nih.gov/nuccore/13937413?report=genbank"> <pmap/>
test