Difference between revisions of "OpenInsulin"
| Line 41: | Line 41: | ||
===Current status=== | ===Current status=== | ||
| − | Clones grown and finally sequence confirmation has come through.<br> | + | Clones grown in DH5alphas and finally sequence confirmation has come through.<br> |
| + | |||
Complications from low copy number vector and GC-rich stretches overcome via midipreps and 'power read upgrades' for the sequencing.<br> | Complications from low copy number vector and GC-rich stretches overcome via midipreps and 'power read upgrades' for the sequencing.<br> | ||
Looks great...<br> | Looks great...<br> | ||
| Line 47: | Line 48: | ||
and mini vs midipreps<br> | and mini vs midipreps<br> | ||
| − | seq traces are clean for (lengths)<br> | + | seq [traces] are clean for (lengths)<br> |
| + | |||
| + | |||
| + | |||
| + | == Photos == | ||
| + | <gallery mode="traditional"> | ||
| + | |||
| + | *File:OpenInsulinMidiPrepsplusH3smaller.png| gel with H3 digest and midi vs miniprep | ||
| + | File:WinsulinGC.png | GC rich seqs | ||
| + | File:Ppro.png | ProInsulin construct | ||
| + | File:Pwin.png | Winsulin construct | ||
| + | |||
| + | |||
| + | </gallery> | ||
==Partners - You?== | ==Partners - You?== | ||
Revision as of 14:49, 7 February 2021
The Open Insulin project is part of an international collaboration.
At Hackuarium, the first results are just beginning:
We are happy to have sequence confirmation of two OpenInsulin plasmids from Alex Kelly
Winsulin and ProInsulin
to produce and test (+ controls)
The project has been under discussion for quite some time, and we hope more people will want to join in for this work.
test
part1
Our goals
- Pave the way for more OpenInsulin tests with International Colleagues
- Demystify and explain the best practices toward any open science project
- Produce Winsulin and ProInsulin for open access positive controls
Why participatory research?
the means to confront anti-science sentiments is to pull more people in
learning through hands on experiences is crucial...
Why Open Data?
By making all the data generated by the project open, we want to explore and promote an alternative to proprietary initiatives. Open data supports literacy and fosters innovation by both citizens and scholars. Open designs can still be produced for commercial ventures, and can make profits if there is a market for the product.
What are the specific challenges of the project and has anyone done this before?
Open Insulin history
Hackuarium's Open Insulin Entanglements
Alex Kelly to Geneva
Plasmids finally to Hackuarium, around August 2020, for Winsulin and ProInsulin, both constructs based on periplasmic expression vectors.
Current status
Clones grown in DH5alphas and finally sequence confirmation has come through.
Complications from low copy number vector and GC-rich stretches overcome via midipreps and 'power read upgrades' for the sequencing.
Looks great...
gel with HindIII diagnostic result.
and mini vs midipreps
seq [traces] are clean for (lengths)
Photos
- *File:OpenInsulinMidiPrepsplusH3smaller.png
gel with H3 digest and midi vs miniprep
GC rich seqs
ProInsulin construct
Winsulin construct
