AGiR! for genomic integrity/cometassaydev

This is the page where lab notes will be uploaded from runs of the cheek cell comet assay protocol...

Experiments

• Cheek Cell comets

Conditions

• tests of pbs vs saline
• tests for optimal numbers of cells
• tests of filtration vs gravity for eliminating big clumps of cells (1st attempt with lab 40micron filter run 8march2017, with Anna and Bastain)
• tests for staining dyes
• control conditions - negative control with no protease treatment, positive control with H2O2

Current 'best' protocol

More to come soon!!