Difference between revisions of "AGiR! for genomic integrity/cometassaydev"
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*tests of pbs vs saline | *tests of pbs vs saline | ||
*tests for optimal numbers of cells | *tests for optimal numbers of cells | ||
| + | *tests of filtration vs gravity for eliminating big clumps of cells (1st attempt with lab 40micron filter run 8march2017, with Anna and Bastain) | ||
*tests for staining dyes | *tests for staining dyes | ||
*control conditions - negative control with no protease treatment, positive control with H2O2 | *control conditions - negative control with no protease treatment, positive control with H2O2 | ||
Revision as of 11:32, 11 March 2017
This is the page where lab notes will be uploaded from runs of the cheek cell comet assay protocol...
Experiments
- Cheek Cell comets
Conditions
- tests of pbs vs saline
- tests for optimal numbers of cells
- tests of filtration vs gravity for eliminating big clumps of cells (1st attempt with lab 40micron filter run 8march2017, with Anna and Bastain)
- tests for staining dyes
- control conditions - negative control with no protease treatment, positive control with H2O2
Current 'best' protocol
More to come soon!!