AGiR! for genomic integrity

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Genomic integrity is a new 'big picture' concept to aid public health efforts and basically includes all the molecular genetic details of cells! So many things we commonly choose to do can impact genomic integrity, also potentially affecting future generations!

At the Hackuarium, AGiR! hopes to really get some citizen science action going - developing DIYbio methods to readily assay *your very own* cells for DNA damage, using classic (circa 1980s) techniques. If these can still be sold in 'kits,' maybe it will also be simple to make them available to all for open source efforts?
Here is the basic plan: using inner cheek cells for micronuclei and comet assays!
Watch this space for more info and visit
the AGiR! site


Introduction

Context

This is a part of a new Hackuarium project on genomic integrity This wiki page will help keep track of development of simple protocols for two methods around which to build experiments related to simple quantitation of DNA damage in human cells.

People

Contact and lead: Rachel Aronoff

Participants: maybe you?!

Objectives

Objective
Setup in short

Background & Inspiration

Here is the link to the Prezi Rachel presented (13apr16) at an Open hackuarium night! Prezi on DIYbio for Genomic integrity!

Some theory needed ?

more on comet cells and micronuclei to come soon!

Inspiration / References / Similar projects

Inspiration: good pages to come

  • [http://]
  • [Link 2]


Setup description

General principle:

Materials & budget

  • What, size | quantity | where to get it | price / unit


(If it’s an experiment) Parameters

To get baselines first

then To vary | Values

p1 |
p2 |
Constants (Key elements of set-up)
c1 |
c2
To measure/ observe
m1
m2

Protocol (Plan)

Startup

Phase 1

do what | frequency | duration

Lab Notebook & News (Real)

We put our measures here: [link to other doc?]

Run / prototype #2

What's new compared to #1:

Results / Feedback from use

Recommendation + next steps